Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
175	0.007291	0.1466	b1498	ydeN	-8.19	0.139530	95 -- 124	45 -- 67	putative Ser-type periplasmic non-aryl sulfatase	b3678 b3801	_____________94_____________________________125\n_____________\|______________________________\|\n5'-AUU...CUUUU______________CUAUCUCU________CUAAC...UAA-3'\n______________UUUAGCUAAAUCUG________UUAUGUGA\n______________\|\|:\|\|\|:\|\|\|\|\|:\|________\|\|\|:\|\|\|\|\n______________AAGUCGGUUUAGGC________AAUGCACU\n3'-AUU...UUUAU______________U_______________UCAUU...AUG-5'\n_____________\|______________________________\|\n_____________68_____________________________44\n	118 -- 124	45 -- 51	-22.2428 kcal/mol	5.90754 kcal/mol	8.16749 kcal/mol	94 125@LB@ \| \|@LB@5'-AUU...CUUUU CUAUCUCU CUAAC...UAA-3'@LB@ UUUAGCUAAAUCUG UUAUGUGA@LB@ \|\|:\|\|\|:\|\|\|\|\|:\| \|\|\|:\|\|\|\|@LB@ AAGUCGGUUUAGGC AAUGCACU@LB@3'-AUU...UUUAU U UCAUU...AUG-5'@LB@ \| \|@LB@ 68 44@LB@@LB@@LB@Energy -8.19 kcal/mol@LB@Hybridization Energy -22.2428 kcal/mol@LB@Unfolding Energy - mRNA 5.90754 kcal/mol@LB@Unfolding Energy - sRNA 8.16749 kcal/mol@LB@@LB@Position - mRNA 95 -- 124@LB@Position - sRNA 45 -- 67@LB@Position Seed - mRNA 118 -- 124@LB@Position Seed - sRNA 45 -- 51@LB@
65	0.001093	0.05856	b1519	tam	-11.83	0.011992	187 -- 231	1 -- 50	trans-aconitate methyltransferase		_____________186____________________________________________________232\n_____________\|______________________________________________________\|\n5'-UUU...CGCUG_____________AAAU______C_________C__________U___A_____ACUUU...AAU-3'\n______________ACG_GGAGUAAAA____GUCUGA____UGGAAC______CCUCU_UAU_UCUAC\n______________\|\|\|_\|:\|\|\|\|\|\|\|____\|\|\|\|\|\|____\|:\|\|\|\|______\|\|\|\|\|_\|\|:_:\|\|\|\|\n______________UGC_CUUCAUUUU____CAGACU____AUCUUG______GGAGA_AUG_GGAUG\n3'-AUU...GCUAA___A_________C_________UUCU______UAGAAU_____C_________-5'\n_____________\|_____________________________________________________\|\n_____________51____________________________________________________1\n	191 -- 197	39 -- 45	-32.7669 kcal/mol	8.27841 kcal/mol	12.6772 kcal/mol	186 232@LB@ \| \|@LB@5'-UUU...CGCUG AAAU C C U A ACUUU...AAU-3'@LB@ ACG GGAGUAAAA GUCUGA UGGAAC CCUCU UAU UCUAC@LB@ \|\|\| \|:\|\|\|\|\|\|\| \|\|\|\|\|\| \|:\|\|\|\| \|\|\|\|\| \|\|: :\|\|\|\|@LB@ UGC CUUCAUUUU CAGACU AUCUUG GGAGA AUG GGAUG@LB@3'-AUU...GCUAA A C UUCU UAGAAU C -5'@LB@ \| \|@LB@ 51 1@LB@@LB@@LB@Energy -11.83 kcal/mol@LB@Hybridization Energy -32.7669 kcal/mol@LB@Unfolding Energy - mRNA 8.27841 kcal/mol@LB@Unfolding Energy - sRNA 12.6772 kcal/mol@LB@@LB@Position - mRNA 187 -- 231@LB@Position - sRNA 1 -- 50@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 39 -- 45@LB@
189	0.008373	0.1558	b1559	quuQ	-11.90	0.011421	197 -- 219	1 -- 24	Qin prophage; putative antitermination protein Q		_____________196______________________220\n_____________\|________________________\|\n5'-UCU...AGAAC______GA______GAA_______CAAAA...AUG-3'\n______________GAGCAU___ACCUU___GCCUUAC\n______________\|\|:\|\|\|___\|\|\|\|:___:\|\|:\|\|\|\n______________CUUGUA___UGGAG___UGGGAUG\n3'-AUU...UCUAU______GAA_____ACA_______-5'\n_____________\|_______________________\|\n_____________25______________________1\n	213 -- 219	1 -- 7	-21.9861 kcal/mol	2.0909 kcal/mol	8.02158 kcal/mol	196 220@LB@ \| \|@LB@5'-UCU...AGAAC GA GAA CAAAA...AUG-3'@LB@ GAGCAU ACCUU GCCUUAC@LB@ \|\|:\|\|\| \|\|\|\|: :\|\|:\|\|\|@LB@ CUUGUA UGGAG UGGGAUG@LB@3'-AUU...UCUAU GAA ACA -5'@LB@ \| \|@LB@ 25 1@LB@@LB@@LB@Energy -11.90 kcal/mol@LB@Hybridization Energy -21.9861 kcal/mol@LB@Unfolding Energy - mRNA 2.0909 kcal/mol@LB@Unfolding Energy - sRNA 8.02158 kcal/mol@LB@@LB@Position - mRNA 197 -- 219@LB@Position - sRNA 1 -- 24@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 1 -- 7@LB@
126	0.00422	0.1178	b1621	malX	-14.96	0.000732	43 -- 87	1 -- 56	maltose and glucose-specific PTS enzyme IIB component and IIC component		_____________42__________________________________________________________88\n_____________\|___________________________________________________________\|\n5'-AUG...ACUCC____________C___________________A______________UAUU________ACCCG...GCA-3'\n______________CUGAUUAUGUGA_______________AGAUA_AACGUUUUACCUUU____UAUCUUAU\n______________\|:\|\|\|\|\|:\|\|\|\|_______________\|\|\|\|\|_\|\|\|:\|:\|\|\|\|\|\|:\|____\|\|:\|:\|\|:\n______________GGCUAAUGCACU_______________UCUAU_UUGUAGAAUGGAGA____AUGGGAUG\n3'-AUU...GUUUA____________UCAUUUUCCAGACUU_____C______________C___________-5'\n_____________\|__________________________________________________________\|\n_____________57_________________________________________________________1\n	81 -- 87	1 -- 7	-37.7188 kcal/mol	10.08 kcal/mol	12.7022 kcal/mol	42 88@LB@ \| \|@LB@5'-AUG...ACUCC C A UAUU ACCCG...GCA-3'@LB@ CUGAUUAUGUGA AGAUA AACGUUUUACCUUU UAUCUUAU@LB@ \|:\|\|\|\|\|:\|\|\|\| \|\|\|\|\| \|\|\|:\|:\|\|\|\|\|\|:\| \|\|:\|:\|\|:@LB@ GGCUAAUGCACU UCUAU UUGUAGAAUGGAGA AUGGGAUG@LB@3'-AUU...GUUUA UCAUUUUCCAGACUU C C -5'@LB@ \| \|@LB@ 57 1@LB@@LB@@LB@Energy -14.96 kcal/mol@LB@Hybridization Energy -37.7188 kcal/mol@LB@Unfolding Energy - mRNA 10.08 kcal/mol@LB@Unfolding Energy - sRNA 12.7022 kcal/mol@LB@@LB@Position - mRNA 43 -- 87@LB@Position - sRNA 1 -- 56@LB@Position Seed - mRNA 81 -- 87@LB@Position Seed - sRNA 1 -- 7@LB@
146	0.005604	0.1342	b1637	tyrS	-10.18	0.040102	30 -- 71	2 -- 50	tyrosyl-tRNA synthetase		_____________29________________________________________________72\n_____________\|_________________________________________________\|\n5'-CAU...UACCA_______AAU___U_CG________U__U____________________AAAAG...CGA-3'\n______________GCGUGAA____GA_G__UGGAAGAU_GA_CGUCUUGC______ACCCUG\n______________:\|\|\|\|\|\|____:\|_\|__\|\|:\|\|\|\|\|_\|\|_\|:\|\|\|\|:\|______\|\|\|\|\|:\n______________UGCACUU____UU_C__ACUUUCUA_CU_GUAGAAUG______UGGGAU\n3'-AUU...GCUAA_______CAUU__C_AG________U__U________GAGACA______G-5'\n_____________\|_________________________________________________\|\n_____________51________________________________________________1\n	30 -- 36	44 -- 50	-35.883 kcal/mol	13.0482 kcal/mol	12.6769 kcal/mol	29 72@LB@ \| \|@LB@5'-CAU...UACCA AAU U CG U U AAAAG...CGA-3'@LB@ GCGUGAA GA G UGGAAGAU GA CGUCUUGC ACCCUG@LB@ :\|\|\|\|\|\| :\| \| \|\|:\|\|\|\|\| \|\| \|:\|\|\|\|:\| \|\|\|\|\|:@LB@ UGCACUU UU C ACUUUCUA CU GUAGAAUG UGGGAU@LB@3'-AUU...GCUAA CAUU C AG U U GAGACA G-5'@LB@ \| \|@LB@ 51 1@LB@@LB@@LB@Energy -10.18 kcal/mol@LB@Hybridization Energy -35.883 kcal/mol@LB@Unfolding Energy - mRNA 13.0482 kcal/mol@LB@Unfolding Energy - sRNA 12.6769 kcal/mol@LB@@LB@Position - mRNA 30 -- 71@LB@Position - sRNA 2 -- 50@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 44 -- 50@LB@
165	0.006672	0.1423	b1651	gloA	-7.94	0.160716	101 -- 108	49 -- 56	glyoxalase I Ni-dependent		_____________100______109\n_____________\|________\|\n5'-UGG...CUAUA________CCGAC...AAA-3'\n______________CCGAUUAC\n______________\|\|\|\|\|\|\|\|\n______________GGCUAAUG\n3'-AUU...GUUUA________CACUU...AUG-5'\n_____________\|________\|\n_____________57_______48\n	102 -- 108	49 -- 55	-10.6899 kcal/mol	2.44421 kcal/mol	0.317884 kcal/mol	100 109@LB@ \| \|@LB@5'-UGG...CUAUA CCGAC...AAA-3'@LB@ CCGAUUAC@LB@ \|\|\|\|\|\|\|\|@LB@ GGCUAAUG@LB@3'-AUU...GUUUA CACUU...AUG-5'@LB@ \| \|@LB@ 57 48@LB@@LB@@LB@Energy -7.94 kcal/mol@LB@Hybridization Energy -10.6899 kcal/mol@LB@Unfolding Energy - mRNA 2.44421 kcal/mol@LB@Unfolding Energy - sRNA 0.317884 kcal/mol@LB@@LB@Position - mRNA 101 -- 108@LB@Position - sRNA 49 -- 56@LB@Position Seed - mRNA 102 -- 108@LB@Position Seed - sRNA 49 -- 55@LB@
144	0.005273	0.1288	b1652	rnt	-8.26	0.134019	203 -- 213	48 -- 58	RNase T; exoribonuclease T; structured DNA 3' exonuclease; RNA processing; DNA repair		_____________202_________214\n_____________\|___________\|\n5'-AAG...CAGAU_______A___CUCAA...CCG-3'\n______________GUCCGAU_ACG\n______________:\|\|\|\|\|\|_\|\|\|\n______________UAGGCUA_UGC\n3'-AUU...CGGUU_______A___ACUUC...AUG-5'\n_____________\|___________\|\n_____________59__________47\n	203 -- 209	52 -- 58	-12.2442 kcal/mol	2.4043 kcal/mol	1.55987 kcal/mol	202 214@LB@ \| \|@LB@5'-AAG...CAGAU A CUCAA...CCG-3'@LB@ GUCCGAU ACG@LB@ :\|\|\|\|\|\| \|\|\|@LB@ UAGGCUA UGC@LB@3'-AUU...CGGUU A ACUUC...AUG-5'@LB@ \| \|@LB@ 59 47@LB@@LB@@LB@Energy -8.26 kcal/mol@LB@Hybridization Energy -12.2442 kcal/mol@LB@Unfolding Energy - mRNA 2.4043 kcal/mol@LB@Unfolding Energy - sRNA 1.55987 kcal/mol@LB@@LB@Position - mRNA 203 -- 213@LB@Position - sRNA 48 -- 58@LB@Position Seed - mRNA 203 -- 209@LB@Position Seed - sRNA 52 -- 58@LB@
134	0.004727	0.1241	b1655	mepH	-9.70	0.055155	231 -- 243	1 -- 13	murein DD-endopeptidase space-maker hydrolase		_____________230___________244\n_____________\|_____________\|\n5'-CGU...UCACG_____________UUUUU...AGA-3'\n______________CUCUGUGCUUUAC\n______________\|\|\|\|\|\|:\|::\|\|\|\n______________GAGACAUGGGAUG\n3'-AUU...GAAUG_____________-5'\n_____________\|____________\|\n_____________14___________1\n	237 -- 243	1 -- 7	-18.9465 kcal/mol	4.13106 kcal/mol	5.13393 kcal/mol	230 244@LB@ \| \|@LB@5'-CGU...UCACG UUUUU...AGA-3'@LB@ CUCUGUGCUUUAC@LB@ \|\|\|\|\|\|:\|::\|\|\|@LB@ GAGACAUGGGAUG@LB@3'-AUU...GAAUG -5'@LB@ \| \|@LB@ 14 1@LB@@LB@@LB@Energy -9.70 kcal/mol@LB@Hybridization Energy -18.9465 kcal/mol@LB@Unfolding Energy - mRNA 4.13106 kcal/mol@LB@Unfolding Energy - sRNA 5.13393 kcal/mol@LB@@LB@Position - mRNA 231 -- 243@LB@Position - sRNA 1 -- 13@LB@Position Seed - mRNA 237 -- 243@LB@Position Seed - sRNA 1 -- 7@LB@
66	0.001146	0.05856	b1657	ydhP	-3.95	0.782289	131 -- 139	49 -- 57	putative MFS transporter inner membrane protein		_____________130_______140\n_____________\|_________\|\n5'-UCG...UUUUU_G_______UGUCA...GCG-3'\n______________U_UGAUUGC\n______________\|_:\|\|\|\|:\|\n______________A_GCUAAUG\n3'-AUU...GGUUU_G_______CACUU...AUG-5'\n_____________\|_________\|\n_____________58________48\n	133 -- 139	49 -- 55	-6.30818 kcal/mol	2.04371 kcal/mol	0.318327 kcal/mol	130 140@LB@ \| \|@LB@5'-UCG...UUUUU G UGUCA...GCG-3'@LB@ U UGAUUGC@LB@ \| :\|\|\|\|:\|@LB@ A GCUAAUG@LB@3'-AUU...GGUUU G CACUU...AUG-5'@LB@ \| \|@LB@ 58 48@LB@@LB@@LB@Energy -3.95 kcal/mol@LB@Hybridization Energy -6.30818 kcal/mol@LB@Unfolding Energy - mRNA 2.04371 kcal/mol@LB@Unfolding Energy - sRNA 0.318327 kcal/mol@LB@@LB@Position - mRNA 131 -- 139@LB@Position - sRNA 49 -- 57@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 49 -- 55@LB@
193	0.008653	0.1558	b1677	lpp	-9.16	0.078007	273 -- 298	53 -- 76	murein lipoprotein		_____________272________________________299\n_____________\|__________________________\|\n5'-UCA...GCAAC________CGA______GUCU_____CG-3'\n______________GCUAAAAU___UCAGCU____UCUGA\n______________\|\|\|\|\|\|\|\|___\|\|\|\|\|:____\|\|:\|\|\n______________CGAUUUUA___AGUCGG____AGGCU\n3'-AUU...CCCGC________UA_______UUU______AAUGC...AUG-5'\n_____________\|__________________________\|\n_____________77_________________________52\n	273 -- 279	70 -- 76	-19.09 kcal/mol	3.58418 kcal/mol	6.36489 kcal/mol	272 299@LB@ \| \|@LB@5'-UCA...GCAAC CGA GUCU CG-3'@LB@ GCUAAAAU UCAGCU UCUGA@LB@ \|\|\|\|\|\|\|\| \|\|\|\|\|: \|\|:\|\|@LB@ CGAUUUUA AGUCGG AGGCU@LB@3'-AUU...CCCGC UA UUU AAUGC...AUG-5'@LB@ \| \|@LB@ 77 52@LB@@LB@@LB@Energy -9.16 kcal/mol@LB@Hybridization Energy -19.09 kcal/mol@LB@Unfolding Energy - mRNA 3.58418 kcal/mol@LB@Unfolding Energy - sRNA 6.36489 kcal/mol@LB@@LB@Position - mRNA 273 -- 298@LB@Position - sRNA 53 -- 76@LB@Position Seed - mRNA 273 -- 279@LB@Position Seed - sRNA 70 -- 76@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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