| 7 |
0.0001858 |
0.08823 |
bsu34210 |
yvfG |
-17.07 |
0.012126 |
85 -- 142 |
32 -- 86 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________84____________________________________________________________143\n_____________|_____________________________________________________________|\n5'-GAG...GUUGA______AAUAC___________C____GUU__A__________GCUCAC____________CAUUG...ACU-3'\n______________GGCUUU_____UUGUA__CUUG_UGAA___UC_UAUCCAUCUU______UCCCC_UUUCUU\n______________|||||:_____|||:|__||||_::||___||_||:||||||:______|||||_||||||\n______________CCGAAG_____AACGU__GAAC_GUUU___AG_AUGGGUAGAG______AGGGG_AAAGAA\n3'-AUU...ACUUA______C_________UA____C____GUU__C__________AAUA_______U______ACUUU...CAA-5'\n_____________|_____________________________________________________________|\n_____________87____________________________________________________________31\n |
119 -- 125 |
48 -- 54 |
-40.7387 kcal/mol |
11.2598 kcal/mol |
14.8836 kcal/mol |
84 143@LB@ | |@LB@5'-GAG...GUUGA AAUAC C GUU A GCUCAC CAUUG...ACU-3'@LB@ GGCUUU UUGUA CUUG UGAA UC UAUCCAUCUU UCCCC UUUCUU@LB@ |||||: |||:| |||| ::|| || ||:||||||: ||||| ||||||@LB@ CCGAAG AACGU GAAC GUUU AG AUGGGUAGAG AGGGG AAAGAA@LB@3'-AUU...ACUUA C UA C GUU C AAUA U ACUUU...CAA-5'@LB@ | |@LB@ 87 31@LB@@LB@@LB@Energy -17.07 kcal/mol@LB@Hybridization Energy -40.7387 kcal/mol@LB@Unfolding Energy - mRNA 11.2598 kcal/mol@LB@Unfolding Energy - sRNA 14.8836 kcal/mol@LB@@LB@Position - mRNA 85 -- 142@LB@Position - sRNA 32 -- 86@LB@Position Seed - mRNA 119 -- 125@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 27 |
0.0007779 |
0.1059 |
bsu31480 |
yuxJ |
-17.06 |
0.012234 |
48 -- 110 |
4 -- 55 |
MFS transporter |
|
_____________47______________________________________________________________111\n_____________|_______________________________________________________________|\n5'-UGC...AAUUC___U____GACAU___________CUAA____AA____UUUUCCCAGC_____C_________AAAUU...UUG-3'\n______________CCC_UCUC_____UUCUUCAUUUU____AAAU__UGAA__________CGCUU_GUUUCUUUU\n______________|||_||||_____|||::|||||:____||||__||||__________|||||_:||||||||\n______________GGG_AGAG_____AAGGGGUAAAG____UUUA__ACUU__________GCGAA_UAAAGAAAA\n3'-AUU...AGCAU___U____AAU_____________AAAC____A_____UU_____________U_________CAA-5'\n_____________|_______________________________________________________________|\n_____________56______________________________________________________________3\n |
104 -- 110 |
4 -- 10 |
-33.1771 kcal/mol |
7.88293 kcal/mol |
11.7347 kcal/mol |
47 111@LB@ | |@LB@5'-UGC...AAUUC U GACAU CUAA AA UUUUCCCAGC C AAAUU...UUG-3'@LB@ CCC UCUC UUCUUCAUUUU AAAU UGAA CGCUU GUUUCUUUU@LB@ ||| |||| |||::|||||: |||| |||| ||||| :||||||||@LB@ GGG AGAG AAGGGGUAAAG UUUA ACUU GCGAA UAAAGAAAA@LB@3'-AUU...AGCAU U AAU AAAC A UU U CAA-5'@LB@ | |@LB@ 56 3@LB@@LB@@LB@Energy -17.06 kcal/mol@LB@Hybridization Energy -33.1771 kcal/mol@LB@Unfolding Energy - mRNA 7.88293 kcal/mol@LB@Unfolding Energy - sRNA 11.7347 kcal/mol@LB@@LB@Position - mRNA 48 -- 110@LB@Position - sRNA 4 -- 55@LB@Position Seed - mRNA 104 -- 110@LB@Position Seed - sRNA 4 -- 10@LB@ |
| 158 |
0.007267 |
0.1751 |
bsu33000 |
htrB |
-17.04 |
0.012352 |
105 -- 237 |
50 -- 180 |
serine protease Do-like HtrB |
|
_____________104_____________________________238\n_____________|_______________________________|\n5'-UUU...GUGCU_______UCUCC______U______A_____ACUUG...CUG-3'\n______________UUGCUUU_____UCAUUA_UGGGAC_AUUGA\n______________||:||:|_____||||:|_::||||_|||||\n______________AAUGAGA_____AGUAGU_GUCCUG_UAACU\n3'-AUU...AAAAA__________________U____________GUAAC...CAA-5'\n_____________|_______________________________|\n_____________181_____________________________49\n |
110 -- 116 |
164 -- 170 |
-21.2082 kcal/mol |
7.59468 kcal/mol |
7.14638 kcal/mol |
104 238@LB@ | |@LB@5'-UUU...GUGCU UCUCC U A ACUUG...CUG-3'@LB@ UUGCUUU UCAUUA UGGGAC AUUGA@LB@ ||:||:| ||||:| ::|||| |||||@LB@ AAUGAGA AGUAGU GUCCUG UAACU@LB@3'-AUU...AAAAA U GUAAC...CAA-5'@LB@ | |@LB@ 181 49@LB@@LB@@LB@Energy -17.04 kcal/mol@LB@Hybridization Energy -21.2082 kcal/mol@LB@Unfolding Energy - mRNA 7.59468 kcal/mol@LB@Unfolding Energy - sRNA 7.14638 kcal/mol@LB@@LB@Position - mRNA 105 -- 237@LB@Position - sRNA 50 -- 180@LB@Position Seed - mRNA 110 -- 116@LB@Position Seed - sRNA 164 -- 170@LB@ |
| 9 |
0.0002071 |
0.08823 |
bsu08425 |
mprF |
-16.98 |
0.012672 |
3 -- 50 |
34 -- 81 |
phosphatidylglycerol lysyltransferase |
|
_____________2_______________________________________________51\n_____________|_______________________________________________|\n_________5'-AC__________GU_______U__AU__________AUCAC________CAUUC...AUC-3'\n______________UGUUGU_AUC___GUAAAU_AU____UCCAUUUC_____UUCCUCAU\n______________:||||:_|||___|:|||:_||____:||||:||_____||||:|||\n______________GCAACG_UAG___CGUUUG_UA____GGGUAGAG_____AAGGGGUA\n3'-AUU...CCGAA______U___AAC______U__GCAU________AAU__________AAGAA...CAA-5'\n_____________|_______________________________________________|\n_____________82______________________________________________33\n |
39 -- 45 |
37 -- 43 |
-29.0114 kcal/mol |
1.80779 kcal/mol |
13.626 kcal/mol |
2 51@LB@ | |@LB@ 5'-AC GU U AU AUCAC CAUUC...AUC-3'@LB@ UGUUGU AUC GUAAAU AU UCCAUUUC UUCCUCAU@LB@ :||||: ||| |:|||: || :||||:|| ||||:|||@LB@ GCAACG UAG CGUUUG UA GGGUAGAG AAGGGGUA@LB@3'-AUU...CCGAA U AAC U GCAU AAU AAGAA...CAA-5'@LB@ | |@LB@ 82 33@LB@@LB@@LB@Energy -16.98 kcal/mol@LB@Hybridization Energy -29.0114 kcal/mol@LB@Unfolding Energy - mRNA 1.80779 kcal/mol@LB@Unfolding Energy - sRNA 13.626 kcal/mol@LB@@LB@Position - mRNA 3 -- 50@LB@Position - sRNA 34 -- 81@LB@Position Seed - mRNA 39 -- 45@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 182 |
0.008397 |
0.1751 |
bsu38410 |
sacX |
-16.96 |
0.012854 |
69 -- 151 |
101 -- 169 |
negative regulator of SacY activity |
|
_____________68_____________________________________________________________________________________152\n_____________|______________________________________________________________________________________|\n5'-ACA...UCUCA_____CG_________C_______U________AUUUU____C___AUUUCUCACUUUU_____UU____A______AC_______UUGAU...UAA-3'\n______________UGCUC__UCGUCGACG_GGGCAUU____UUGUC_____CAGC_GGU_____________UUCAU____UG_UUGUCA__UUUUCAU\n______________|:|||__||:||:||:_||:||||____|||||_____||||_:||_____________|:|||____||_||||||__||:||||\n______________AUGAG__AGUAGUUGU_CCUGUAA____AACAG_____GUCG_UCA_____________AGGUA____AC_AACAGU__AAGAGUA\n3'-AUU...AAAAA_____A__________________CUGU_____GC_______U_____________________UAUU_________GA_______GAAGU...CAA-5'\n_____________|______________________________________________________________________________________|\n_____________170____________________________________________________________________________________100\n |
143 -- 149 |
103 -- 109 |
-47.5037 kcal/mol |
18.4019 kcal/mol |
14.5039 kcal/mol |
68 152@LB@ | |@LB@5'-ACA...UCUCA CG C U AUUUU C AUUUCUCACUUUU UU A AC UUGAU...UAA-3'@LB@ UGCUC UCGUCGACG GGGCAUU UUGUC CAGC GGU UUCAU UG UUGUCA UUUUCAU@LB@ |:||| ||:||:||: ||:|||| ||||| |||| :|| |:||| || |||||| ||:||||@LB@ AUGAG AGUAGUUGU CCUGUAA AACAG GUCG UCA AGGUA AC AACAGU AAGAGUA@LB@3'-AUU...AAAAA A CUGU GC U UAUU GA GAAGU...CAA-5'@LB@ | |@LB@ 170 100@LB@@LB@@LB@Energy -16.96 kcal/mol@LB@Hybridization Energy -47.5037 kcal/mol@LB@Unfolding Energy - mRNA 18.4019 kcal/mol@LB@Unfolding Energy - sRNA 14.5039 kcal/mol@LB@@LB@Position - mRNA 69 -- 151@LB@Position - sRNA 101 -- 169@LB@Position Seed - mRNA 143 -- 149@LB@Position Seed - sRNA 103 -- 109@LB@ |
| 24 |
0.0006259 |
0.1011 |
bsu03380 |
yckB |
-16.82 |
0.013754 |
20 -- 129 |
16 -- 148 |
ABC transporter substrate-binding protein |
|
_____________19_________________________________________________________________________________________________________________________________________________130\n_____________|__________________________________________________________________________________________________________________________________________________|\n5'-UGC...UCCUU__U____C_______CA___U___A________UUUUUUCA_______C_____CC_____U__AC_______________________________CCC________G_____UGUAUA_A__A___GAACUU____________GAUCA...CCA-3'\n______________GG_ACGU_UGGCAUU__UCC_UGG_CAA_UUCC___________UCAC__UCUU__CUUCA_AG_________GCUUU_____________UAAACA_____ACCCAU_CUUUU______C_CA_UUC_______UUUGAAAACGC\n______________||_||:|_||:||||__|||_::|_|||_||||___________||||__|||:__|||||_||_________||||:_____________:|||||_____||||||_||:||______|_||_|||_______|||||||||||\n______________CC_UGUA_ACUGUAA__AGG_GUC_GUU_AAGG___________AGUG__AGAG__GAAGU_UC_________CGAAG_____________GUUUGU_____UGGGUA_GAGAA______G_GU_AAG_______AAACUUUUGCG\n3'-AUU...GUUGU_______________C____C_______C____UAUAUUACAAC____AA____UA_____U__AUUACUUAC_____CAACGUUAGAACC______UAGCA____________UAAGG_____A___AAACUUU___________AAUUA...CAA-5'\n_____________|__________________________________________________________________________________________________________________________________________________|\n_____________149________________________________________________________________________________________________________________________________________________15\n |
123 -- 129 |
16 -- 22 |
-61.2162 kcal/mol |
13.3554 kcal/mol |
33.3199 kcal/mol |
19 130@LB@ | |@LB@5'-UGC...UCCUU U C CA U A UUUUUUCA C CC U AC CCC G UGUAUA A A GAACUU GAUCA...CCA-3'@LB@ GG ACGU UGGCAUU UCC UGG CAA UUCC UCAC UCUU CUUCA AG GCUUU UAAACA ACCCAU CUUUU C CA UUC UUUGAAAACGC@LB@ || ||:| ||:|||| ||| ::| ||| |||| |||| |||: ||||| || ||||: :||||| |||||| ||:|| | || ||| |||||||||||@LB@ CC UGUA ACUGUAA AGG GUC GUU AAGG AGUG AGAG GAAGU UC CGAAG GUUUGU UGGGUA GAGAA G GU AAG AAACUUUUGCG@LB@3'-AUU...GUUGU C C C UAUAUUACAAC AA UA U AUUACUUAC CAACGUUAGAACC UAGCA UAAGG A AAACUUU AAUUA...CAA-5'@LB@ | |@LB@ 149 15@LB@@LB@@LB@Energy -16.82 kcal/mol@LB@Hybridization Energy -61.2162 kcal/mol@LB@Unfolding Energy - mRNA 13.3554 kcal/mol@LB@Unfolding Energy - sRNA 33.3199 kcal/mol@LB@@LB@Position - mRNA 20 -- 129@LB@Position - sRNA 16 -- 148@LB@Position Seed - mRNA 123 -- 129@LB@Position Seed - sRNA 16 -- 22@LB@ |
| 165 |
0.007704 |
0.1751 |
bsu18170 |
yngA |
-16.77 |
0.014091 |
132 -- 192 |
122 -- 181 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________131_____________________________________________193\n_____________|_______________________________________________|\n5'-ACA...CAUAU_________UGUU____CA___C_______UAU______________CAACA...CGC-3'\n______________UUUAUUAAU____UAUU__ACA_UGUCCGC___AGUAA__UCCAUAU\n______________||:||:||:____:|||__|||_|||||||___||:||__|||||||\n______________AAGUAGUUG____GUAA__UGU_ACAGGCG___UCGUU__AGGUAUA\n3'-AUU...AUGAG_________UCCU____C____A_______________CA_______UUACA...CAA-5'\n_____________|_______________________________________________|\n_____________182_____________________________________________121\n |
186 -- 192 |
122 -- 128 |
-30.4083 kcal/mol |
4.20108 kcal/mol |
10.3807 kcal/mol |
131 193@LB@ | |@LB@5'-ACA...CAUAU UGUU CA C UAU CAACA...CGC-3'@LB@ UUUAUUAAU UAUU ACA UGUCCGC AGUAA UCCAUAU@LB@ ||:||:||: :||| ||| ||||||| ||:|| |||||||@LB@ AAGUAGUUG GUAA UGU ACAGGCG UCGUU AGGUAUA@LB@3'-AUU...AUGAG UCCU C A CA UUACA...CAA-5'@LB@ | |@LB@ 182 121@LB@@LB@@LB@Energy -16.77 kcal/mol@LB@Hybridization Energy -30.4083 kcal/mol@LB@Unfolding Energy - mRNA 4.20108 kcal/mol@LB@Unfolding Energy - sRNA 10.3807 kcal/mol@LB@@LB@Position - mRNA 132 -- 192@LB@Position - sRNA 122 -- 181@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 122 -- 128@LB@ |
| 95 |
0.003784 |
0.1541 |
bsu12910 |
proG |
-16.76 |
0.014152 |
1 -- 50 |
33 -- 83 |
pyrroline-5-carboxylate reductase 3 |
|
______________1______________________________________________________51\n______________|______________________________________________________|\n___________5'-________U_________UC_____A_U________A_______A_U________CUAUU...UUG-3'\n______________UUCGUU_C_GUUUU__CA__ACGAU_G_UAUCCAUC___UGUUC_C_CCGUUUCU\n______________||||||_|_:|:||__||__||:||_|_||:|||||___|:|||_|_||:|||||\n______________AAGCAA_G_UAGAA__GU__UGUUA_C_AUGGGUAG___AUAAG_G_GGUAAAGA\n3'-AUU...UACCG______C_U_____CC__U______G__________AGA________________AACUU...CAA-5'\n_____________|_______________________________________________________|\n_____________84______________________________________________________32\n |
44 -- 50 |
33 -- 39 |
-32.4348 kcal/mol |
4.82786 kcal/mol |
13.9589 kcal/mol |
1 51@LB@ | |@LB@ 5'- U UC A U A A U CUAUU...UUG-3'@LB@ UUCGUU C GUUUU CA ACGAU G UAUCCAUC UGUUC C CCGUUUCU@LB@ |||||| | :|:|| || ||:|| | ||:||||| |:||| | ||:|||||@LB@ AAGCAA G UAGAA GU UGUUA C AUGGGUAG AUAAG G GGUAAAGA@LB@3'-AUU...UACCG C U CC U G AGA AACUU...CAA-5'@LB@ | |@LB@ 84 32@LB@@LB@@LB@Energy -16.76 kcal/mol@LB@Hybridization Energy -32.4348 kcal/mol@LB@Unfolding Energy - mRNA 4.82786 kcal/mol@LB@Unfolding Energy - sRNA 13.9589 kcal/mol@LB@@LB@Position - mRNA 1 -- 50@LB@Position - sRNA 33 -- 83@LB@Position Seed - mRNA 44 -- 50@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 172 |
0.008071 |
0.1751 |
bsu03860 |
ycnD |
-16.76 |
0.014170 |
153 -- 167 |
135 -- 149 |
FMN reductase |
|
_____________152_____________168\n_____________|_______________|\n5'-CGC...UCCUC_______________CAGAG...CGG-3'\n______________UUGAUGUUGUCCGCA\n______________||||::|||||||||\n______________AACUGUAACAGGCGU\n3'-AUU...CCUGU_______________CGUUC...CAA-5'\n_____________|_______________|\n_____________150_____________134\n |
161 -- 167 |
135 -- 141 |
-23.0656 kcal/mol |
2.48067 kcal/mol |
5.52377 kcal/mol |
152 168@LB@ | |@LB@5'-CGC...UCCUC CAGAG...CGG-3'@LB@ UUGAUGUUGUCCGCA@LB@ ||||::|||||||||@LB@ AACUGUAACAGGCGU@LB@3'-AUU...CCUGU CGUUC...CAA-5'@LB@ | |@LB@ 150 134@LB@@LB@@LB@Energy -16.76 kcal/mol@LB@Hybridization Energy -23.0656 kcal/mol@LB@Unfolding Energy - mRNA 2.48067 kcal/mol@LB@Unfolding Energy - sRNA 5.52377 kcal/mol@LB@@LB@Position - mRNA 153 -- 167@LB@Position - sRNA 135 -- 149@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 135 -- 141@LB@ |
| 78 |
0.002646 |
0.1315 |
bsu37410 |
albE |
-16.69 |
0.014616 |
33 -- 81 |
32 -- 77 |
antilisterial bacteriocin subtilosin biosynthesis protein AlbE |
|
_____________32_________________________________________________82\n_____________|__________________________________________________|\n5'-CUU...AUGGA_______CU_____AU____GU_______G__UUGUC______UA_____AGCCA...AAA-3'\n______________GCGGUUU___CAAA__AUCG__CUUAUCU_UU_____UUCCCC__UUCUU\n______________||::|:|___||||__||||__|::||||_||_____||||||__|||||\n______________CGUUAGA___GUUU__UAGC__GGGUAGA_AA_____AAGGGG__AAGAA\n3'-AUU...AGCAA_______ACC____GU____AU_______G__U__________UA_____ACUUU...CAA-5'\n_____________|__________________________________________________|\n_____________78_________________________________________________31\n |
54 -- 60 |
49 -- 55 |
-34.8992 kcal/mol |
7.30498 kcal/mol |
13.8943 kcal/mol |
32 82@LB@ | |@LB@5'-CUU...AUGGA CU AU GU G UUGUC UA AGCCA...AAA-3'@LB@ GCGGUUU CAAA AUCG CUUAUCU UU UUCCCC UUCUU@LB@ ||::|:| |||| |||| |::|||| || |||||| |||||@LB@ CGUUAGA GUUU UAGC GGGUAGA AA AAGGGG AAGAA@LB@3'-AUU...AGCAA ACC GU AU G U UA ACUUU...CAA-5'@LB@ | |@LB@ 78 31@LB@@LB@@LB@Energy -16.69 kcal/mol@LB@Hybridization Energy -34.8992 kcal/mol@LB@Unfolding Energy - mRNA 7.30498 kcal/mol@LB@Unfolding Energy - sRNA 13.8943 kcal/mol@LB@@LB@Position - mRNA 33 -- 81@LB@Position - sRNA 32 -- 77@LB@Position Seed - mRNA 54 -- 60@LB@Position Seed - sRNA 49 -- 55@LB@ |
