Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
103	0.003241	0.1101	b0837	yliI	-8.40	0.123505	223 -- 230	4 -- 11	soluble aldose sugar dehydrogenase	_____________222______231\n_____________\|________\|\n5'-GUC...UUUCC________CUUAU...AAC-3'\n______________UUGUGCCC\n______________:\|\|\|:\|\|\|\n______________GACAUGGG\n3'-AUU...AUGGA________AUG-5'\n_____________\|________\|\n_____________12_______3\n	224 -- 230	4 -- 10	-12.324 kcal/mol	0.491988 kcal/mol	3.45361 kcal/mol	222 231@LB@ \| \|@LB@5'-GUC...UUUCC CUUAU...AAC-3'@LB@ UUGUGCCC@LB@ :\|\|\|:\|\|\|@LB@ GACAUGGG@LB@3'-AUU...AUGGA AUG-5'@LB@ \| \|@LB@ 12 3@LB@@LB@@LB@Energy -8.40 kcal/mol@LB@Hybridization Energy -12.324 kcal/mol@LB@Unfolding Energy - mRNA 0.491988 kcal/mol@LB@Unfolding Energy - sRNA 3.45361 kcal/mol@LB@@LB@Position - mRNA 223 -- 230@LB@Position - sRNA 4 -- 11@LB@Position Seed - mRNA 224 -- 230@LB@Position Seed - sRNA 4 -- 10@LB@
104	0.003257	0.1101	b0858	ybjO	-8.07	0.149394	228 -- 257	2 -- 30	DUF2593 family inner membrane protein	_____________227______________________________258\n_____________\|________________________________\|\n5'-AUG...UUUUU____A__________C_____A__CU_G____AAUGU...UUA-3'\n______________AAGA___AACAUCUU_AUCUC_UG__C_CCUG\n______________\|\|\|\|___\|\|\|\|\|\|\|\|_\|:\|\|\|_\|\|__\|_\|\|\|:\n______________UUCU___UUGUAGAA_UGGAG_AC__G_GGAU\n3'-AUU...AGACU____AUC_________________AU______G-5'\n_____________\|________________________________\|\n_____________31_______________________________1\n	234 -- 240	16 -- 22	-20.5501 kcal/mol	4.45089 kcal/mol	8.05308 kcal/mol	227 258@LB@ \| \|@LB@5'-AUG...UUUUU A C A CU G AAUGU...UUA-3'@LB@ AAGA AACAUCUU AUCUC UG C CCUG@LB@ \|\|\|\| \|\|\|\|\|\|\|\| \|:\|\|\| \|\| \| \|\|\|:@LB@ UUCU UUGUAGAA UGGAG AC G GGAU@LB@3'-AUU...AGACU AUC AU G-5'@LB@ \| \|@LB@ 31 1@LB@@LB@@LB@Energy -8.07 kcal/mol@LB@Hybridization Energy -20.5501 kcal/mol@LB@Unfolding Energy - mRNA 4.45089 kcal/mol@LB@Unfolding Energy - sRNA 8.05308 kcal/mol@LB@@LB@Position - mRNA 228 -- 257@LB@Position - sRNA 2 -- 30@LB@Position Seed - mRNA 234 -- 240@LB@Position Seed - sRNA 16 -- 22@LB@
154	0.006224	0.1406	b0882	clpA	-11.36	0.017252	127 -- 158	2 -- 32	ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease chaperone activity	_____________126______________________________159\n_____________\|________________________________\|\n5'-UUU...CGCGA______AU_______CAUUG__G_____G___GAAAA...CGC-3'\n______________GGGAGA__GAGCAUC_____CU_UGUAC_CUA\n______________\|::\|\|\|__\|\|:\|\|\|\|_____\|\|_\|\|\|\|\|_\|\|\|\n______________CUUUCU__CUUGUAG_____GA_ACAUG_GAU\n3'-AUU...CCAGA______AU_______AAUG___G_____G___G-5'\n_____________\|________________________________\|\n_____________33_______________________________1\n	135 -- 141	18 -- 24	-27.9684 kcal/mol	7.80814 kcal/mol	8.82442 kcal/mol	126 159@LB@ \| \|@LB@5'-UUU...CGCGA AU CAUUG G G GAAAA...CGC-3'@LB@ GGGAGA GAGCAUC CU UGUAC CUA@LB@ \|::\|\|\| \|\|:\|\|\|\| \|\| \|\|\|\|\| \|\|\|@LB@ CUUUCU CUUGUAG GA ACAUG GAU@LB@3'-AUU...CCAGA AU AAUG G G G-5'@LB@ \| \|@LB@ 33 1@LB@@LB@@LB@Energy -11.36 kcal/mol@LB@Hybridization Energy -27.9684 kcal/mol@LB@Unfolding Energy - mRNA 7.80814 kcal/mol@LB@Unfolding Energy - sRNA 8.82442 kcal/mol@LB@@LB@Position - mRNA 127 -- 158@LB@Position - sRNA 2 -- 32@LB@Position Seed - mRNA 135 -- 141@LB@Position Seed - sRNA 18 -- 24@LB@
25	0.0002052	0.02687	b0885	aat	-14.70	0.000956	173 -- 191	42 -- 59	leucyl/phenylalanyl-tRNA-protein transferase	_____________172_________________192\n_____________\|___________________\|\n5'-CGU...UGAAC_______C___________UUUGC...UUA-3'\n______________GAUCCGA_UUGCGUGGAGU\n______________:\|\|\|\|\|\|_\|\|:\|\|\|\|:\|\|\|\n______________UUAGGCU_AAUGCACUUCA\n3'-AUU...UCGGU___________________UUUUC...AUG-5'\n_____________\|___________________\|\n_____________60__________________41\n	185 -- 191	42 -- 48	-23.9715 kcal/mol	6.00825 kcal/mol	3.27976 kcal/mol	172 192@LB@ \| \|@LB@5'-CGU...UGAAC C UUUGC...UUA-3'@LB@ GAUCCGA UUGCGUGGAGU@LB@ :\|\|\|\|\|\| \|\|:\|\|\|\|:\|\|\|@LB@ UUAGGCU AAUGCACUUCA@LB@3'-AUU...UCGGU UUUUC...AUG-5'@LB@ \| \|@LB@ 60 41@LB@@LB@@LB@Energy -14.70 kcal/mol@LB@Hybridization Energy -23.9715 kcal/mol@LB@Unfolding Energy - mRNA 6.00825 kcal/mol@LB@Unfolding Energy - sRNA 3.27976 kcal/mol@LB@@LB@Position - mRNA 173 -- 191@LB@Position - sRNA 42 -- 59@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 42 -- 48@LB@
70	0.001199	0.05856	b0954	fabA	-10.37	0.035200	228 -- 254	4 -- 31	beta-hydroxydecanoyl thioester dehydrase	_____________227__________________________255\n_____________\|____________________________\|\n5'-GUG...AUACA_________C_U___________GU_G_GGUGA...ACA-3'\n______________AAAGA_AGA_C_UCUUGCCUCUG__C_C\n______________\|\|\|\|\|_\|\|\|_\|_\|\|\|\|:\|\|\|\|\|\|__\|_\|\n______________UUUCU_UCU_G_AGAAUGGAGAC__G_G\n3'-AUU...CAGAC_____A___U_U___________AU_G_AUG-5'\n_____________\|____________________________\|\n_____________32___________________________3\n	243 -- 249	9 -- 15	-26.2676 kcal/mol	7.86183 kcal/mol	8.05676 kcal/mol	227 255@LB@ \| \|@LB@5'-GUG...AUACA C U GU G GGUGA...ACA-3'@LB@ AAAGA AGA C UCUUGCCUCUG C C@LB@ \|\|\|\|\| \|\|\| \| \|\|\|\|:\|\|\|\|\|\| \| \|@LB@ UUUCU UCU G AGAAUGGAGAC G G@LB@3'-AUU...CAGAC A U U AU G AUG-5'@LB@ \| \|@LB@ 32 3@LB@@LB@@LB@Energy -10.37 kcal/mol@LB@Hybridization Energy -26.2676 kcal/mol@LB@Unfolding Energy - mRNA 7.86183 kcal/mol@LB@Unfolding Energy - sRNA 8.05676 kcal/mol@LB@@LB@Position - mRNA 228 -- 254@LB@Position - sRNA 4 -- 31@LB@Position Seed - mRNA 243 -- 249@LB@Position Seed - sRNA 9 -- 15@LB@
106	0.003365	0.1101	b0955	ycbZ	-10.52	0.031644	269 -- 288	48 -- 66	putative peptidase	_____________268__________________289\n_____________\|____________________\|\n5'-UUG...UUUGC_______C_________A__AAAAC...CUU-3'\n______________UCAGCCA_AUUUGAUUG_CG\n______________\|\|\|\|\|\|\|_\|\|::\|\|\|\|:_\|\|\n______________AGUCGGU_UAGGCUAAU_GC\n3'-AUU...UUAUA_______U____________ACUUC...AUG-5'\n_____________\|____________________\|\n_____________67___________________47\n	279 -- 285	50 -- 56	-21.5915 kcal/mol	3.60671 kcal/mol	7.48271 kcal/mol	268 289@LB@ \| \|@LB@5'-UUG...UUUGC C A AAAAC...CUU-3'@LB@ UCAGCCA AUUUGAUUG CG@LB@ \|\|\|\|\|\|\| \|\|::\|\|\|\|: \|\|@LB@ AGUCGGU UAGGCUAAU GC@LB@3'-AUU...UUAUA U ACUUC...AUG-5'@LB@ \| \|@LB@ 67 47@LB@@LB@@LB@Energy -10.52 kcal/mol@LB@Hybridization Energy -21.5915 kcal/mol@LB@Unfolding Energy - mRNA 3.60671 kcal/mol@LB@Unfolding Energy - sRNA 7.48271 kcal/mol@LB@@LB@Position - mRNA 269 -- 288@LB@Position - sRNA 48 -- 66@LB@Position Seed - mRNA 279 -- 285@LB@Position Seed - sRNA 50 -- 56@LB@
108	0.003398	0.1101	b0973	hyaB	-11.40	0.016694	267 -- 275	48 -- 56	hydrogenase 1 large subunit	_____________266_______276\n_____________\|_________\|\n5'-GGU...UCGAC_________CGCAU...GCG-3'\n______________CCGAUUACG\n______________\|\|\|\|\|\|\|\|\|\n______________GGCUAAUGC\n3'-AUU...GUUUA_________ACUUC...AUG-5'\n_____________\|_________\|\n_____________57________47\n	269 -- 275	48 -- 54	-14.3171 kcal/mol	1.33562 kcal/mol	1.55919 kcal/mol	266 276@LB@ \| \|@LB@5'-GGU...UCGAC CGCAU...GCG-3'@LB@ CCGAUUACG@LB@ \|\|\|\|\|\|\|\|\|@LB@ GGCUAAUGC@LB@3'-AUU...GUUUA ACUUC...AUG-5'@LB@ \| \|@LB@ 57 47@LB@@LB@@LB@Energy -11.40 kcal/mol@LB@Hybridization Energy -14.3171 kcal/mol@LB@Unfolding Energy - mRNA 1.33562 kcal/mol@LB@Unfolding Energy - sRNA 1.55919 kcal/mol@LB@@LB@Position - mRNA 267 -- 275@LB@Position - sRNA 48 -- 56@LB@Position Seed - mRNA 269 -- 275@LB@Position Seed - sRNA 48 -- 54@LB@
116	0.003844	0.1165	b1004	wrbA	-12.42	0.007537	123 -- 145	10 -- 35	NAD(P)H:quinone oxidoreductase	_____________122________________________146\n_____________\|__________________________\|\n5'-UUA...UGCAC__________________________AAAUA...AAG-3'\n______________UCUGAAA___AGGACGUCUUAUCUUU\n______________\|\|\|\|\|\|\|___\|\|:\|\|:\|\|\|\|\|:\|\|:\|\n______________AGACUUU___UCUUGUAGAAUGGAGA\n3'-AUU...UUUCC_______CUA________________CAUGG...AUG-5'\n_____________\|__________________________\|\n_____________36_________________________9\n	133 -- 139	16 -- 22	-25.4666 kcal/mol	4.32254 kcal/mol	8.70229 kcal/mol	122 146@LB@ \| \|@LB@5'-UUA...UGCAC AAAUA...AAG-3'@LB@ UCUGAAA AGGACGUCUUAUCUUU@LB@ \|\|\|\|\|\|\| \|\|:\|\|:\|\|\|\|\|:\|\|:\|@LB@ AGACUUU UCUUGUAGAAUGGAGA@LB@3'-AUU...UUUCC CUA CAUGG...AUG-5'@LB@ \| \|@LB@ 36 9@LB@@LB@@LB@Energy -12.42 kcal/mol@LB@Hybridization Energy -25.4666 kcal/mol@LB@Unfolding Energy - mRNA 4.32254 kcal/mol@LB@Unfolding Energy - sRNA 8.70229 kcal/mol@LB@@LB@Position - mRNA 123 -- 145@LB@Position - sRNA 10 -- 35@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 16 -- 22@LB@
96	0.002764	0.1002	b1034	ycdX	-5.77	0.451964	102 -- 115	32 -- 46	alkaline phosphatase	_____________101_____________116\n_____________\|_______________\|\n5'-GGC...AAAAA_______________CGGGC...AAC-3'\n______________GGGG_AGAGGGUCUG\n______________\|::\|_\|:\|:\|\|\|\|\|\|\n______________CUUC_UUUUCCAGAC\n3'-AUU...AUGCA____A__________UUUCU...AUG-5'\n_____________\|_______________\|\n_____________47______________31\n	109 -- 115	32 -- 38	-16.8058 kcal/mol	3.54072 kcal/mol	7.24668 kcal/mol	101 116@LB@ \| \|@LB@5'-GGC...AAAAA CGGGC...AAC-3'@LB@ GGGG AGAGGGUCUG@LB@ \|::\| \|:\|:\|\|\|\|\|\|@LB@ CUUC UUUUCCAGAC@LB@3'-AUU...AUGCA A UUUCU...AUG-5'@LB@ \| \|@LB@ 47 31@LB@@LB@@LB@Energy -5.77 kcal/mol@LB@Hybridization Energy -16.8058 kcal/mol@LB@Unfolding Energy - mRNA 3.54072 kcal/mol@LB@Unfolding Energy - sRNA 7.24668 kcal/mol@LB@@LB@Position - mRNA 102 -- 115@LB@Position - sRNA 32 -- 46@LB@Position Seed - mRNA 109 -- 115@LB@Position Seed - sRNA 32 -- 38@LB@
115	0.00375	0.1147	b1057	yceJ	-5.38	0.522888	37 -- 59	1 -- 23	putative cytochrome b561	_____________36_______________________60\n_____________\|________________________\|\n5'-UGA...CACCA_______C__AUG___A_______UAAAC...UGU-3'\n______________GACAUCU_UG___CUG_ACCC_AC\n______________:\|\|\|\|\|\|_\|:___\|\|\|_\|\|\|\|_\|\|\n______________UUGUAGA_AU___GAC_UGGG_UG\n3'-AUU...CUAUC__________GGA___A____A__-5'\n_____________\|_______________________\|\n_____________24______________________1\n	37 -- 43	17 -- 23	-17.2281 kcal/mol	3.85652 kcal/mol	8.01506 kcal/mol	36 60@LB@ \| \|@LB@5'-UGA...CACCA C AUG A UAAAC...UGU-3'@LB@ GACAUCU UG CUG ACCC AC@LB@ :\|\|\|\|\|\| \|: \|\|\| \|\|\|\| \|\|@LB@ UUGUAGA AU GAC UGGG UG@LB@3'-AUU...CUAUC GGA A A -5'@LB@ \| \|@LB@ 24 1@LB@@LB@@LB@Energy -5.38 kcal/mol@LB@Hybridization Energy -17.2281 kcal/mol@LB@Unfolding Energy - mRNA 3.85652 kcal/mol@LB@Unfolding Energy - sRNA 8.01506 kcal/mol@LB@@LB@Position - mRNA 37 -- 59@LB@Position - sRNA 1 -- 23@LB@Position Seed - mRNA 37 -- 43@LB@Position Seed - sRNA 17 -- 23@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching