Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
12	3.867e-05	0.01134	b0728	sucC	-9.87	0.049323	145 -- 186	43 -- 73	succinyl-CoA synthetase beta subunit		_____________144_________________________________________187\n_____________\|___________________________________________\|\n5'-CGC...GAUAC_____________UACGGUUUAAA____AA_____________GAUGG...AAG-3'\n______________GAAAUAUUCGGUC___________AGAU__CGAUUAC_UGAAG\n______________:\|\|\|\|\|\|\|\|:\|:\|___________\|:\|\|__\|\|\|\|\|\|\|_\|\|\|\|\|\n______________UUUUAUAAGUCGG___________UUUA__GCUAAUG_ACUUC\n3'-AUU...GCCGA____________________________G________C_____AUUUU...AUG-5'\n_____________\|___________________________________________\|\n_____________74__________________________________________42\n	175 -- 181	49 -- 55	-26.5157 kcal/mol	7.48621 kcal/mol	9.17828 kcal/mol	144 187@LB@ \| \|@LB@5'-CGC...GAUAC UACGGUUUAAA AA GAUGG...AAG-3'@LB@ GAAAUAUUCGGUC AGAU CGAUUAC UGAAG@LB@ :\|\|\|\|\|\|\|\|:\|:\| \|:\|\| \|\|\|\|\|\|\| \|\|\|\|\|@LB@ UUUUAUAAGUCGG UUUA GCUAAUG ACUUC@LB@3'-AUU...GCCGA G C AUUUU...AUG-5'@LB@ \| \|@LB@ 74 42@LB@@LB@@LB@Energy -9.87 kcal/mol@LB@Hybridization Energy -26.5157 kcal/mol@LB@Unfolding Energy - mRNA 7.48621 kcal/mol@LB@Unfolding Energy - sRNA 9.17828 kcal/mol@LB@@LB@Position - mRNA 145 -- 186@LB@Position - sRNA 43 -- 73@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 49 -- 55@LB@
150	0.00611	0.1406	b0735	ybgE	-10.35	0.035619	38 -- 71	5 -- 31	putative inner membrane protein in cydABX-ybgE operon		_____________37_________________________________72\n_____________\|__________________________________\|\n5'-CUG...CCAAA_______UU______AAC___CACUC________AAGUA...UUU-3'\n______________GAAGAUA__GAACGU___ACC_____UCUGUACU\n______________:\|\|\|\|\|\|__\|\|\|\|:\|___\|\|\|_____\|\|\|\|\|\|\|:\n______________UUUCUAU__CUUGUA___UGG_____AGACAUGG\n3'-AUU...CAGAC_______________GAA________________GAUG-5'\n_____________\|__________________________________\|\n_____________32_________________________________4\n	65 -- 71	5 -- 11	-23.0678 kcal/mol	4.68737 kcal/mol	8.05162 kcal/mol	37 72@LB@ \| \|@LB@5'-CUG...CCAAA UU AAC CACUC AAGUA...UUU-3'@LB@ GAAGAUA GAACGU ACC UCUGUACU@LB@ :\|\|\|\|\|\| \|\|\|\|:\| \|\|\| \|\|\|\|\|\|\|:@LB@ UUUCUAU CUUGUA UGG AGACAUGG@LB@3'-AUU...CAGAC GAA GAUG-5'@LB@ \| \|@LB@ 32 4@LB@@LB@@LB@Energy -10.35 kcal/mol@LB@Hybridization Energy -23.0678 kcal/mol@LB@Unfolding Energy - mRNA 4.68737 kcal/mol@LB@Unfolding Energy - sRNA 8.05162 kcal/mol@LB@@LB@Position - mRNA 38 -- 71@LB@Position - sRNA 5 -- 31@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 5 -- 11@LB@
1	0	0	b0757	galK	-15.06	0.000658	183 -- 231	8 -- 60	galactokinase		_____________182_____________________________________________________232\n_____________\|_______________________________________________________\|\n5'-CAU...UUCGC_______GA______U___AAAU_____________AAA____CA__________UUGCC...GUG-3'\n______________GAAUCCG_____GUG_AAG____GAG_UCUGAAAGA___AACA____AUCUCUGU\n______________:\|\|\|\|\|\|_____\|\|\|_\|\|\|____:\|\|_\|\|\|\|\|\|\|\|\|___\|\|\|\|____\|:\|\|\|\|\|\|\n______________UUUAGGC_____CAC_UUC____UUC_AGACUUUCU___UUGU____UGGAGACA\n3'-AUU...GUCGG_______UAAUG_______AUU____C_________AUC____AGAA________UGGGAUG-5'\n_____________\|_______________________________________________________\|\n_____________61______________________________________________________7\n	225 -- 231	8 -- 14	-35.0906 kcal/mol	7.3474 kcal/mol	12.6967 kcal/mol	182 232@LB@ \| \|@LB@5'-CAU...UUCGC GA U AAAU AAA CA UUGCC...GUG-3'@LB@ GAAUCCG GUG AAG GAG UCUGAAAGA AACA AUCUCUGU@LB@ :\|\|\|\|\|\| \|\|\| \|\|\| :\|\| \|\|\|\|\|\|\|\|\| \|\|\|\| \|:\|\|\|\|\|\|@LB@ UUUAGGC CAC UUC UUC AGACUUUCU UUGU UGGAGACA@LB@3'-AUU...GUCGG UAAUG AUU C AUC AGAA UGGGAUG-5'@LB@ \| \|@LB@ 61 7@LB@@LB@@LB@Energy -15.06 kcal/mol@LB@Hybridization Energy -35.0906 kcal/mol@LB@Unfolding Energy - mRNA 7.3474 kcal/mol@LB@Unfolding Energy - sRNA 12.6967 kcal/mol@LB@@LB@Position - mRNA 183 -- 231@LB@Position - sRNA 8 -- 60@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 8 -- 14@LB@
98	0.002849	0.1023	b0772	ybhC	-6.17	0.383539	27 -- 33	49 -- 55	acyl-CoA thioesterase lipoprotein		_____________26______34\n_____________\|_______\|\n5'-UCA...CGCCU_______UGCGA...AUC-3'\n______________CGAUUAC\n______________\|\|\|\|\|\|\|\n______________GCUAAUG\n3'-AUU...UUUAG_______CACUU...AUG-5'\n_____________\|_______\|\n_____________56______48\n	27 -- 33	49 -- 55	-7.86935 kcal/mol	1.39337 kcal/mol	0.311712 kcal/mol	26 34@LB@ \| \|@LB@5'-UCA...CGCCU UGCGA...AUC-3'@LB@ CGAUUAC@LB@ \|\|\|\|\|\|\|@LB@ GCUAAUG@LB@3'-AUU...UUUAG CACUU...AUG-5'@LB@ \| \|@LB@ 56 48@LB@@LB@@LB@Energy -6.17 kcal/mol@LB@Hybridization Energy -7.86935 kcal/mol@LB@Unfolding Energy - mRNA 1.39337 kcal/mol@LB@Unfolding Energy - sRNA 0.311712 kcal/mol@LB@@LB@Position - mRNA 27 -- 33@LB@Position - sRNA 49 -- 55@LB@Position Seed - mRNA 27 -- 33@LB@Position Seed - sRNA 49 -- 55@LB@
23	0.0001777	0.02687	b0774	bioA	-12.72	0.005870	209 -- 228	5 -- 24	7 8-diaminopelargonic acid synthase PLP-dependent		_____________208___________________229\n_____________\|_____________________\|\n5'-ACG...ACAAC____G________________AACGC...AAG-3'\n______________GGAC_AUCUUGCCUUUG_ACC\n______________\|:\|\|_\|\|\|\|\|:\|\|\|:\|\|_\|\|\|\n______________CUUG_UAGAAUGGAGAC_UGG\n3'-AUU...UCUAU_________________A___GAUG-5'\n_____________\|_____________________\|\n_____________25____________________4\n	219 -- 225	9 -- 15	-24.4474 kcal/mol	3.74608 kcal/mol	8.01095 kcal/mol	208 229@LB@ \| \|@LB@5'-ACG...ACAAC G AACGC...AAG-3'@LB@ GGAC AUCUUGCCUUUG ACC@LB@ \|:\|\| \|\|\|\|\|:\|\|\|:\|\| \|\|\|@LB@ CUUG UAGAAUGGAGAC UGG@LB@3'-AUU...UCUAU A GAUG-5'@LB@ \| \|@LB@ 25 4@LB@@LB@@LB@Energy -12.72 kcal/mol@LB@Hybridization Energy -24.4474 kcal/mol@LB@Unfolding Energy - mRNA 3.74608 kcal/mol@LB@Unfolding Energy - sRNA 8.01095 kcal/mol@LB@@LB@Position - mRNA 209 -- 228@LB@Position - sRNA 5 -- 24@LB@Position Seed - mRNA 219 -- 225@LB@Position Seed - sRNA 9 -- 15@LB@
125	0.004186	0.1178	b0781	moaA	-8.90	0.091954	79 -- 99	4 -- 23	molybdopterin biosynthesis protein A		_____________78____________________100\n_____________\|_____________________\|\n5'-ACG...CACUA____C___A_______C____GGGUC...CGG-3'\n______________AACA_UCU_GCCUCUG_ACCU\n______________\|\|\|\|_\|\|\|_:\|\|\|\|\|\|_\|\|\|:\n______________UUGU_AGA_UGGAGAC_UGGG\n3'-AUU...CUAUC________A_______A____AUG-5'\n_____________\|_____________________\|\n_____________24____________________3\n	88 -- 94	9 -- 15	-21.7522 kcal/mol	4.86496 kcal/mol	8.01134 kcal/mol	78 100@LB@ \| \|@LB@5'-ACG...CACUA C A C GGGUC...CGG-3'@LB@ AACA UCU GCCUCUG ACCU@LB@ \|\|\|\| \|\|\| :\|\|\|\|\|\| \|\|\|:@LB@ UUGU AGA UGGAGAC UGGG@LB@3'-AUU...CUAUC A A AUG-5'@LB@ \| \|@LB@ 24 3@LB@@LB@@LB@Energy -8.90 kcal/mol@LB@Hybridization Energy -21.7522 kcal/mol@LB@Unfolding Energy - mRNA 4.86496 kcal/mol@LB@Unfolding Energy - sRNA 8.01134 kcal/mol@LB@@LB@Position - mRNA 79 -- 99@LB@Position - sRNA 4 -- 23@LB@Position Seed - mRNA 88 -- 94@LB@Position Seed - sRNA 9 -- 15@LB@
2	0	0	b0796	ybiH	-7.52	0.201543	119 -- 140	54 -- 76	DUF1956 domain-containing tetR family putative transcriptional regulator	b1013	_____________118_____________________141\n_____________\|_______________________\|\n5'-CGG...GCGUU________UC_______UA____GACAA...CCA-3'\n______________GCUAAAAU__UUAGCCA___CUG\n______________\|\|\|\|\|\|\|\|__\|:\|\|\|\|\|___\|:\|\n______________CGAUUUUA__AGUCGGU___GGC\n3'-AUU...CCCGC________UA_______UUA___UAAUG...AUG-5'\n_____________\|_______________________\|\n_____________77______________________53\n	129 -- 135	60 -- 66	-19.7488 kcal/mol	5.88928 kcal/mol	6.3634 kcal/mol	118 141@LB@ \| \|@LB@5'-CGG...GCGUU UC UA GACAA...CCA-3'@LB@ GCUAAAAU UUAGCCA CUG@LB@ \|\|\|\|\|\|\|\| \|:\|\|\|\|\| \|:\|@LB@ CGAUUUUA AGUCGGU GGC@LB@3'-AUU...CCCGC UA UUA UAAUG...AUG-5'@LB@ \| \|@LB@ 77 53@LB@@LB@@LB@Energy -7.52 kcal/mol@LB@Hybridization Energy -19.7488 kcal/mol@LB@Unfolding Energy - mRNA 5.88928 kcal/mol@LB@Unfolding Energy - sRNA 6.3634 kcal/mol@LB@@LB@Position - mRNA 119 -- 140@LB@Position - sRNA 54 -- 76@LB@Position Seed - mRNA 129 -- 135@LB@Position Seed - sRNA 60 -- 66@LB@
48	0.0005438	0.03933	b0811	glnH	-6.95	0.268265	70 -- 77	1 -- 8	glutamine transporter subunit		_____________69_______78\n_____________\|________\|\n5'-AUC...GGUAU________AAAUC...CCG-3'\n______________UGCCCUAU\n______________\|:\|\|\|\|\|:\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	71 -- 77	1 -- 7	-10.5963 kcal/mol	1.549 kcal/mol	2.11603 kcal/mol	69 78@LB@ \| \|@LB@5'-AUC...GGUAU AAAUC...CCG-3'@LB@ UGCCCUAU@LB@ \|:\|\|\|\|\|:@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -6.95 kcal/mol@LB@Hybridization Energy -10.5963 kcal/mol@LB@Unfolding Energy - mRNA 1.549 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 70 -- 77@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 71 -- 77@LB@Position Seed - sRNA 1 -- 7@LB@
191	0.008587	0.1558	b0823	ybiW	-9.49	0.063221	47 -- 54	1 -- 8	putative pyruvate formate lyase	b3951	_____________46_______55\n_____________\|________\|\n5'-ACC...UUUUC________CACAC...CGC-3'\n______________UGCCCUAC\n______________\|:\|\|\|\|\|\|\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	48 -- 54	1 -- 7	-12.4662 kcal/mol	0.878635 kcal/mol	2.11603 kcal/mol	46 55@LB@ \| \|@LB@5'-ACC...UUUUC CACAC...CGC-3'@LB@ UGCCCUAC@LB@ \|:\|\|\|\|\|\|@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -9.49 kcal/mol@LB@Hybridization Energy -12.4662 kcal/mol@LB@Unfolding Energy - mRNA 0.878635 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 47 -- 54@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 48 -- 54@LB@Position Seed - sRNA 1 -- 7@LB@
187	0.008276	0.1556	b0827	moeA	-6.51	0.330510	82 -- 91	23 -- 32	molybdopterin molybdenumtransferase; molybdopterin biosynthesis protein		_____________81_________92\n_____________\|__________\|\n5'-AUU...AUAUA__________UAUGG...UAC-3'\n______________GAAAGAUGGA\n______________\|\|\|\|\|\|\|:\|\|\n______________CUUUCUAUCU\n3'-AUU...CCAGA__________UGUAG...AUG-5'\n_____________\|__________\|\n_____________33_________22\n	85 -- 91	23 -- 29	-12.1772 kcal/mol	2.37895 kcal/mol	3.23951 kcal/mol	81 92@LB@ \| \|@LB@5'-AUU...AUAUA UAUGG...UAC-3'@LB@ GAAAGAUGGA@LB@ \|\|\|\|\|\|\|:\|\|@LB@ CUUUCUAUCU@LB@3'-AUU...CCAGA UGUAG...AUG-5'@LB@ \| \|@LB@ 33 22@LB@@LB@@LB@Energy -6.51 kcal/mol@LB@Hybridization Energy -12.1772 kcal/mol@LB@Unfolding Energy - mRNA 2.37895 kcal/mol@LB@Unfolding Energy - sRNA 3.23951 kcal/mol@LB@@LB@Position - mRNA 82 -- 91@LB@Position - sRNA 23 -- 32@LB@Position Seed - mRNA 85 -- 91@LB@Position Seed - sRNA 23 -- 29@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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