| 166 |
0.007737 |
0.1751 |
bsu10770 |
wprA |
-9.60 |
0.364837 |
176 -- 232 |
111 -- 164 |
cell wall-associated protease |
|
_____________175_________________________________________________________233\n_____________|___________________________________________________________|\n5'-CUG...GGAGA_______UUUU______G_GAUA____GAAA_____AAAA_____GCUCG______G__GGCAG...GCG-3'\n______________UUCAUUA____GCAGGA_G____ACAU____CGCAG_____UUCA______GUUGU_GC\n______________|||||:|____:|||||_:____||||____|||||_____|:||______|||||_:|\n______________AAGUAGU____UGUCCU_U____UGUA____GCGUC_____AGGU______CAACA_UG\n3'-AUU...AUGAG_________________G_AAC_____ACAG_____GUUCA____AUAUUA_____G__AAAGA...CAA-5'\n_____________|___________________________________________________________|\n_____________165_________________________________________________________110\n |
176 -- 182 |
158 -- 164 |
-36.1081 kcal/mol |
15.1078 kcal/mol |
11.9485 kcal/mol |
175 233@LB@ | |@LB@5'-CUG...GGAGA UUUU G GAUA GAAA AAAA GCUCG G GGCAG...GCG-3'@LB@ UUCAUUA GCAGGA G ACAU CGCAG UUCA GUUGU GC@LB@ |||||:| :||||| : |||| ||||| |:|| ||||| :|@LB@ AAGUAGU UGUCCU U UGUA GCGUC AGGU CAACA UG@LB@3'-AUU...AUGAG G AAC ACAG GUUCA AUAUUA G AAAGA...CAA-5'@LB@ | |@LB@ 165 110@LB@@LB@@LB@Energy -9.60 kcal/mol@LB@Hybridization Energy -36.1081 kcal/mol@LB@Unfolding Energy - mRNA 15.1078 kcal/mol@LB@Unfolding Energy - sRNA 11.9485 kcal/mol@LB@@LB@Position - mRNA 176 -- 232@LB@Position - sRNA 111 -- 164@LB@Position Seed - mRNA 176 -- 182@LB@Position Seed - sRNA 158 -- 164@LB@ |
| 162 |
0.007531 |
0.1751 |
bsu14350 |
yknX |
-8.74 |
0.495402 |
179 -- 195 |
123 -- 140 |
efflux system component YknX |
|
_____________178________________196\n_____________|__________________|\n5'-UGA...UCAAU____________G_____AGCUC...CCG-3'\n______________UCUGUGGCGGGU__CAUA\n______________||:|::||::||__||||\n______________AGGCGUCGUUCA__GUAU\n3'-AUU...GUAAC____________AG____AUUAC...CAA-5'\n_____________|__________________|\n_____________141________________122\n |
184 -- 190 |
129 -- 135 |
-18.5785 kcal/mol |
5.82762 kcal/mol |
6.13212 kcal/mol |
178 196@LB@ | |@LB@5'-UGA...UCAAU G AGCUC...CCG-3'@LB@ UCUGUGGCGGGU CAUA@LB@ ||:|::||::|| ||||@LB@ AGGCGUCGUUCA GUAU@LB@3'-AUU...GUAAC AG AUUAC...CAA-5'@LB@ | |@LB@ 141 122@LB@@LB@@LB@Energy -8.74 kcal/mol@LB@Hybridization Energy -18.5785 kcal/mol@LB@Unfolding Energy - mRNA 5.82762 kcal/mol@LB@Unfolding Energy - sRNA 6.13212 kcal/mol@LB@@LB@Position - mRNA 179 -- 195@LB@Position - sRNA 123 -- 140@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 129 -- 135@LB@ |
| 155 |
0.00708 |
0.1751 |
bsu17280 |
ymaD |
-7.93 |
0.631070 |
192 -- 223 |
42 -- 69 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 16267290; Product type pe : enzyme' |
|
_____________191______________________________224\n_____________|________________________________|\n5'-AAA...AAGGA___A_______G_CAGA_____AU________AAAAG...UUC-3'\n______________GGC_AAACAAU_G____UCAUC__UUUUAUUU\n______________|||_|||||||_|____:||||__|:|||||:\n______________CCG_UUUGUUA_C____GGUAG__AGAAUAAG\n3'-AUU...UAGAA___________G_AUG________________GGGUA...CAA-5'\n_____________|________________________________|\n_____________70_______________________________41\n |
217 -- 223 |
42 -- 48 |
-20.5024 kcal/mol |
4.54925 kcal/mol |
8.89481 kcal/mol |
191 224@LB@ | |@LB@5'-AAA...AAGGA A G CAGA AU AAAAG...UUC-3'@LB@ GGC AAACAAU G UCAUC UUUUAUUU@LB@ ||| ||||||| | :|||| |:|||||:@LB@ CCG UUUGUUA C GGUAG AGAAUAAG@LB@3'-AUU...UAGAA G AUG GGGUA...CAA-5'@LB@ | |@LB@ 70 41@LB@@LB@@LB@Energy -7.93 kcal/mol@LB@Hybridization Energy -20.5024 kcal/mol@LB@Unfolding Energy - mRNA 4.54925 kcal/mol@LB@Unfolding Energy - sRNA 8.89481 kcal/mol@LB@@LB@Position - mRNA 192 -- 223@LB@Position - sRNA 42 -- 69@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 42 -- 48@LB@ |
| 168 |
0.007777 |
0.1751 |
bsu32270 |
yutH |
-11.70 |
0.153975 |
226 -- 259 |
121 -- 158 |
endospore coat-associated protein YutH |
|
_____________225____________________________________260\n_____________|______________________________________|\n5'-UGC...AGAAA____C______C____CAG_______U___________CCAGC...ACU-3'\n______________AAUA_GGCAUU_ACAU_____GCAGC___UUCCAUGUA\n______________||:|_|:||||_||||_____|||||___||||||:||\n______________UUGU_CUGUAA_UGUA_____CGUCG___AAGGUAUAU\n3'-AUU...AGUAG____C______C____ACAGG_____UUC_________UACAA...CAA-5'\n_____________|______________________________________|\n_____________159____________________________________120\n |
253 -- 259 |
121 -- 127 |
-28.4364 kcal/mol |
6.47482 kcal/mol |
10.499 kcal/mol |
225 260@LB@ | |@LB@5'-UGC...AGAAA C C CAG U CCAGC...ACU-3'@LB@ AAUA GGCAUU ACAU GCAGC UUCCAUGUA@LB@ ||:| |:|||| |||| ||||| ||||||:||@LB@ UUGU CUGUAA UGUA CGUCG AAGGUAUAU@LB@3'-AUU...AGUAG C C ACAGG UUC UACAA...CAA-5'@LB@ | |@LB@ 159 120@LB@@LB@@LB@Energy -11.70 kcal/mol@LB@Hybridization Energy -28.4364 kcal/mol@LB@Unfolding Energy - mRNA 6.47482 kcal/mol@LB@Unfolding Energy - sRNA 10.499 kcal/mol@LB@@LB@Position - mRNA 226 -- 259@LB@Position - sRNA 121 -- 158@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 194 |
0.008808 |
0.1751 |
bsu16370 |
fliR |
-9.70 |
0.351361 |
227 -- 250 |
157 -- 179 |
flagellar biosynthetic protein FliR |
|
_____________226____________________________________251\n_____________|______________________________________|\n5'-UAU...CGACA____AA____CAGG________U________UAU____AAAAU...UGC-3'\n______________ACUC___AUC____AACA_GAC_UUGGCGUU___UCCG\n______________||||___|||____||||_|||_|||:|:||___||||\n______________UGAG___UAG____UUGU_CUG_AACUGUAA___AGGC\n3'-AUU...AAAAA____AAG___________C___U________C______GUCGU...CAA-5'\n_____________|______________________________________|\n_____________180____________________________________156\n |
69 -- 75 |
142 -- 148 |
-23.642 kcal/mol |
8.17018 kcal/mol |
7.49537 kcal/mol |
226 251@LB@ | |@LB@5'-UAU...CGACA AA CAGG U UAU AAAAU...UGC-3'@LB@ ACUC AUC AACA GAC UUGGCGUU UCCG@LB@ |||| ||| |||| ||| |||:|:|| ||||@LB@ UGAG UAG UUGU CUG AACUGUAA AGGC@LB@3'-AUU...AAAAA AAG C U C GUCGU...CAA-5'@LB@ | |@LB@ 180 156@LB@@LB@@LB@Energy -9.70 kcal/mol@LB@Hybridization Energy -23.642 kcal/mol@LB@Unfolding Energy - mRNA 8.17018 kcal/mol@LB@Unfolding Energy - sRNA 7.49537 kcal/mol@LB@@LB@Position - mRNA 227 -- 250@LB@Position - sRNA 157 -- 179@LB@Position Seed - mRNA 69 -- 75@LB@Position Seed - sRNA 142 -- 148@LB@ |
| 157 |
0.007221 |
0.1751 |
bsu40660 |
yybF |
-12.55 |
0.105157 |
243 -- 270 |
125 -- 152 |
MFS transporter |
|
_____________242__________________________271\n_____________|____________________________|\n5'-UGU...AAAUU_____U__U__C_____U__________AUACA...AGC-3'\n______________ACGUU_GC_UU_UUCGC_GCGGGUUUCA\n______________||:||_:|_||_|:|||_||::|||:||\n______________UGUAA_UG_AA_AGGCG_CGUUCAAGGU\n3'-AUU...UGUCC_____C__U__C_____U__________AUAUU...CAA-5'\n_____________|____________________________|\n_____________153__________________________124\n |
264 -- 270 |
125 -- 131 |
-24.436 kcal/mol |
4.92439 kcal/mol |
8.98549 kcal/mol |
242 271@LB@ | |@LB@5'-UGU...AAAUU U U C U AUACA...AGC-3'@LB@ ACGUU GC UU UUCGC GCGGGUUUCA@LB@ ||:|| :| || |:||| ||::|||:||@LB@ UGUAA UG AA AGGCG CGUUCAAGGU@LB@3'-AUU...UGUCC C U C U AUAUU...CAA-5'@LB@ | |@LB@ 153 124@LB@@LB@@LB@Energy -12.55 kcal/mol@LB@Hybridization Energy -24.436 kcal/mol@LB@Unfolding Energy - mRNA 4.92439 kcal/mol@LB@Unfolding Energy - sRNA 8.98549 kcal/mol@LB@@LB@Position - mRNA 243 -- 270@LB@Position - sRNA 125 -- 152@LB@Position Seed - mRNA 264 -- 270@LB@Position Seed - sRNA 125 -- 131@LB@ |
| 185 |
0.008526 |
0.1751 |
bsu02370 |
ybgA |
-10.25 |
0.283898 |
252 -- 276 |
59 -- 82 |
GntR family transcriptional regulator |
|
_____________251_________________________277\n_____________|___________________________|\n5'-GAU...UUAAA___G____AAU___A____________AGCUG...UUU-3'\n______________UCG__GCA___AUC__GGCGAAUGAUC\n______________|||__|||___|||__|||:||::|||\n______________AGC__CGU___UAG__CCGUUUGUUAG\n3'-AUU...ACCGA___AA_________AA___________CAUGG...CAA-5'\n_____________|___________________________|\n_____________83__________________________58\n |
266 -- 272 |
63 -- 69 |
-19.3785 kcal/mol |
3.33361 kcal/mol |
6.42004 kcal/mol |
251 277@LB@ | |@LB@5'-GAU...UUAAA G AAU A AGCUG...UUU-3'@LB@ UCG GCA AUC GGCGAAUGAUC@LB@ ||| ||| ||| |||:||::|||@LB@ AGC CGU UAG CCGUUUGUUAG@LB@3'-AUU...ACCGA AA AA CAUGG...CAA-5'@LB@ | |@LB@ 83 58@LB@@LB@@LB@Energy -10.25 kcal/mol@LB@Hybridization Energy -19.3785 kcal/mol@LB@Unfolding Energy - mRNA 3.33361 kcal/mol@LB@Unfolding Energy - sRNA 6.42004 kcal/mol@LB@@LB@Position - mRNA 252 -- 276@LB@Position - sRNA 59 -- 82@LB@Position Seed - mRNA 266 -- 272@LB@Position Seed - sRNA 63 -- 69@LB@ |
| 199 |
0.00902 |
0.1751 |
bsu30990 |
thiT |
-13.50 |
0.067771 |
270 -- 291 |
126 -- 147 |
thiamine transporter ThiT |
|
_____________269_____________________292\n_____________|_______________________|\n5'-AUG...UUUUA______________G__U_____UUAGC...UGC-3'\n______________GACAUUGUCU_CAG_AA_GUUUC\n______________|||||||||:_|||_||_|||:|\n______________CUGUAACAGG_GUC_UU_CAAGG\n3'-AUU...UGUAA__________C___G________UAUAU...CAA-5'\n_____________|_______________________|\n_____________148_____________________125\n |
273 -- 279 |
138 -- 144 |
-23.3567 kcal/mol |
5.16312 kcal/mol |
6.80985 kcal/mol |
269 292@LB@ | |@LB@5'-AUG...UUUUA G U UUAGC...UGC-3'@LB@ GACAUUGUCU CAG AA GUUUC@LB@ |||||||||: ||| || |||:|@LB@ CUGUAACAGG GUC UU CAAGG@LB@3'-AUU...UGUAA C G UAUAU...CAA-5'@LB@ | |@LB@ 148 125@LB@@LB@@LB@Energy -13.50 kcal/mol@LB@Hybridization Energy -23.3567 kcal/mol@LB@Unfolding Energy - mRNA 5.16312 kcal/mol@LB@Unfolding Energy - sRNA 6.80985 kcal/mol@LB@@LB@Position - mRNA 270 -- 291@LB@Position - sRNA 126 -- 147@LB@Position Seed - mRNA 273 -- 279@LB@Position Seed - sRNA 138 -- 144@LB@ |
| 164 |
0.007653 |
0.1751 |
bsu02110 |
ybyB |
-7.82 |
0.651200 |
271 -- 286 |
38 -- 56 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________270_____287\n_____________|_______|\n5'-UUG...AAAAG_______AAAUC...AAU-3'\n______________ACCCGUC\n______________||||:||\n______________UGGGUAG\n3'-AUU...UAGCA_______AGAAU...CAA-5'\n_____________|_______|\n_____________57______37\n |
271 -- 277 |
50 -- 56 |
-11.5721 kcal/mol |
0.816393 kcal/mol |
3.06627 kcal/mol |
270 287@LB@ | |@LB@5'-UUG...AAAAG AAAUC...AAU-3'@LB@ ACCCGUC@LB@ ||||:||@LB@ UGGGUAG@LB@3'-AUU...UAGCA AGAAU...CAA-5'@LB@ | |@LB@ 57 37@LB@@LB@@LB@Energy -7.82 kcal/mol@LB@Hybridization Energy -11.5721 kcal/mol@LB@Unfolding Energy - mRNA 0.816393 kcal/mol@LB@Unfolding Energy - sRNA 3.06627 kcal/mol@LB@@LB@Position - mRNA 271 -- 286@LB@Position - sRNA 38 -- 56@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 50 -- 56@LB@ |
| 188 |
0.008618 |
0.1751 |
bsu31720 |
degQ |
-9.47 |
0.382925 |
276 -- 300 |
147 -- 170 |
degradation enzyme regulation protein DegQ |
|
_____________275______________301\n_____________|________________|\n5'-GAA...AUUCA____GAAA________AAAGC...UUG-3'\n______________UUAC____CAUUAACA\n______________||||____|||:||||\n______________AAUG____GUAGUUGU\n3'-AUU...AAAAA____AGAA________CCUGU...CAA-5'\n_____________|________________|\n_____________171______________146\n |
285 -- 291 |
155 -- 161 |
-9.46814 kcal/mol |
0.995164 kcal/mol |
1.41808 kcal/mol |
275 301@LB@ | |@LB@5'-GAA...AUUCA GAAA AAAGC...UUG-3'@LB@ UUAC CAUUAACA@LB@ |||| |||:||||@LB@ AAUG GUAGUUGU@LB@3'-AUU...AAAAA AGAA CCUGU...CAA-5'@LB@ | |@LB@ 171 146@LB@@LB@@LB@Energy -9.47 kcal/mol@LB@Hybridization Energy -9.46814 kcal/mol@LB@Unfolding Energy - mRNA 0.995164 kcal/mol@LB@Unfolding Energy - sRNA 1.41808 kcal/mol@LB@@LB@Position - mRNA 276 -- 300@LB@Position - sRNA 147 -- 170@LB@Position Seed - mRNA 285 -- 291@LB@Position Seed - sRNA 155 -- 161@LB@ |
