Input and runtime details for job 3202356 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3202356 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:02 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:03:47 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:03:55 CET 2018
	Job Completed		@ Sat Feb 17 02:04:28 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3202356&toolName=CopraRNA ( 30 days expiry )

Description of the job

GcvB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
23	0.0005914	0.08814	stm3466	crp	-13.97	0.171291	272 -- 288	36 -- 50	transcriptional regulator Crp	_____________271_______________________________289\n_____________\|_________________________________\|\n5'-ACG...CGAAA______CUAAA________________C_____UAAUA...AGG-3'\n______________GCUAUG_____ACAGACA____AGAUG_UACAG\n______________:\|\|\|\|\|_____\|\|\|\|\|\|\|____\|:\|\|\|_\|\|\|\|\|\n______________UGAUAC_____UGUCUGU____UUUAC_AUGUC\n3'-UUU...AUUAG______C___________CCCA___________CUUCA...UCA-5'\n_____________\|_________________________________\|\n_____________51________________________________35\n	21 -- 27	155 -- 161	-20.3438 kcal/mol	4.64329 kcal/mol	5.58455 kcal/mol	271 289@LB@ \| \|@LB@5'-ACG...CGAAA CUAAA C UAAUA...AGG-3'@LB@ GCUAUG ACAGACA AGAUG UACAG@LB@ :\|\|\|\|\| \|\|\|\|\|\|\| \|:\|\|\| \|\|\|\|\|@LB@ UGAUAC UGUCUGU UUUAC AUGUC@LB@3'-UUU...AUUAG C CCCA CUUCA...UCA-5'@LB@ \| \|@LB@ 51 35@LB@@LB@@LB@Energy -13.97 kcal/mol@LB@Hybridization Energy -20.3438 kcal/mol@LB@Unfolding Energy - mRNA 4.64329 kcal/mol@LB@Unfolding Energy - sRNA 5.58455 kcal/mol@LB@@LB@Position - mRNA 272 -- 288@LB@Position - sRNA 36 -- 50@LB@Position Seed - mRNA 21 -- 27@LB@Position Seed - sRNA 155 -- 161@LB@
182	0.01014	0.1966	stm3011	galR	-12.96	0.243649	22 -- 54	60 -- 90	transcriptional regulator GalR	_____________21_________________________________________55\n_____________\|__________________________________________\|\n_________5'-AC____C____U____UGCCA______AAUC__________UU_GCAAA...GGC-3'\n______________CUGA_UCGC_GGCC_____GCAGAC____GCAACAGCGC__C\n______________\|\|\|\|_\|\|\|\|_\|:\|\|_____\|\|\|:\|\|____:\|\|\|\|\|:\|:\|__\|\n______________GACU_AGCG_CUGG_____CGUUUG____UGUUGUUGUG__G\n3'-UUU...CACCA____U____U____UUAA_____________________UU_UAGUG...UCA-5'\n_____________\|__________________________________________\|\n_____________91_________________________________________59\n	35 -- 41	75 -- 81	-39.0068 kcal/mol	12.9734 kcal/mol	13.5432 kcal/mol	21 55@LB@ \| \|@LB@ 5'-AC C U UGCCA AAUC UU GCAAA...GGC-3'@LB@ CUGA UCGC GGCC GCAGAC GCAACAGCGC C@LB@ \|\|\|\| \|\|\|\| \|:\|\| \|\|\|:\|\| :\|\|\|\|\|:\|:\| \|@LB@ GACU AGCG CUGG CGUUUG UGUUGUUGUG G@LB@3'-UUU...CACCA U U UUAA UU UAGUG...UCA-5'@LB@ \| \|@LB@ 91 59@LB@@LB@@LB@Energy -12.96 kcal/mol@LB@Hybridization Energy -39.0068 kcal/mol@LB@Unfolding Energy - mRNA 12.9734 kcal/mol@LB@Unfolding Energy - sRNA 13.5432 kcal/mol@LB@@LB@Position - mRNA 22 -- 54@LB@Position - sRNA 60 -- 90@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 75 -- 81@LB@
1	0	0	stm2982	gcvA	-98.53	0.000000	1 -- 73	1 -- 73	transcriptional regulator GcvA	______________1________________________________________________________________________74\n______________\|________________________________________________________________________\|\n___________5'-_________________________________________________________________________GCCGC...CGC-3'\n______________ACAUCACAACCGUAAGCCAAAAGCCCAUCAGAACACGCAUUCCGAUAAAACUUUUCGUUCCGGCUCAGGAAGU\n______________\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\n______________UGUAGUGUUGGCAUUCGGUUUUCGGGUAGUCUUGUGCGUAAGGCUAUUUUGAAAAGCAAGGCCGAGUCCUUCA\n3'-UUU...UGUGU_________________________________________________________________________-5'\n_____________\|________________________________________________________________________\|\n_____________74_______________________________________________________________________1\n	67 -- 73	1 -- 7	-142.883 kcal/mol	17.2949 kcal/mol	26.5914 kcal/mol	1 74@LB@ \| \|@LB@ 5'- GCCGC...CGC-3'@LB@ ACAUCACAACCGUAAGCCAAAAGCCCAUCAGAACACGCAUUCCGAUAAAACUUUUCGUUCCGGCUCAGGAAGU@LB@ \|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|\|@LB@ UGUAGUGUUGGCAUUCGGUUUUCGGGUAGUCUUGUGCGUAAGGCUAUUUUGAAAAGCAAGGCCGAGUCCUUCA@LB@3'-UUU...UGUGU -5'@LB@ \| \|@LB@ 74 1@LB@@LB@@LB@Energy -98.53 kcal/mol@LB@Hybridization Energy -142.883 kcal/mol@LB@Unfolding Energy - mRNA 17.2949 kcal/mol@LB@Unfolding Energy - sRNA 26.5914 kcal/mol@LB@@LB@Position - mRNA 1 -- 73@LB@Position - sRNA 1 -- 73@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 1 -- 7@LB@
159	0.008305	0.1845	stm3625	yhjV	-13.06	0.235438	203 -- 240	55 -- 86	transporter	_____________202________________________241\n_____________\|__________________________\|\n5'-UUA...UAUUU_____________CUCUA________CGACA...UCU-3'\n______________UGGGCAG__GGGG______UGCAGGA\n______________\|\|\|:\|\|\|__:\|\|\|______\|:\|\|\|\|\|\n______________ACCUGUC__UCCC______AUGUCCU\n3'-UUU...GUGAU_______UG____AUUUAC_______UCACU...UCA-5'\n_____________\|__________________________\|\n_____________87_________________________54\n	202 -- 208	139 -- 145	-24.2103 kcal/mol	7.6176 kcal/mol	3.71206 kcal/mol	202 241@LB@ \| \|@LB@5'-UUA...UAUUU CUCUA CGACA...UCU-3'@LB@ UGGGCAG GGGG UGCAGGA@LB@ \|\|\|:\|\|\| :\|\|\| \|:\|\|\|\|\|@LB@ ACCUGUC UCCC AUGUCCU@LB@3'-UUU...GUGAU UG AUUUAC UCACU...UCA-5'@LB@ \| \|@LB@ 87 54@LB@@LB@@LB@Energy -13.06 kcal/mol@LB@Hybridization Energy -24.2103 kcal/mol@LB@Unfolding Energy - mRNA 7.6176 kcal/mol@LB@Unfolding Energy - sRNA 3.71206 kcal/mol@LB@@LB@Position - mRNA 203 -- 240@LB@Position - sRNA 55 -- 86@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 139 -- 145@LB@
171	0.009384	0.1945	stm1741	N/A	-12.47	0.287408	104 -- 168	71 -- 131	transporter	_____________103_________________________________________________________________________169\n_____________\|___________________________________________________________________________\|\n5'-GAG...CGACC____CU__________U______CA______UUC___CCUUAAA_______C___A___U_____G_________AUUCG...UGU-3'\n______________GGUG__GGAAGGG_AG_UUUCGC__UGCCGU___CUG_______GUAGACC_GUU_UAA_CGCAA_GGC_UGACA\n______________:\|\|\|__::\|\|\|\|\|_\|\|_\|:\|\|\|\|__\|:\|\|\|\|___\|\|\|_______\|:\|\|\|\|\|_:\|\|_:\|\|_\|:\|\|\|_::\|_::\|\|\|\n______________UCAC__UUUUCCC_UC_AGAGCG__AUGGCA___GAC_______CGUCUGG_UAA_GUU_GUGUU_UUG_GUUGU\n3'-UUU...GUCCU____U________A_________________CCA___UUAG__________U___C___U_____G___U_____AGUGU...UCA-5'\n_____________\|___________________________________________________________________________\|\n_____________132_________________________________________________________________________70\n	139 -- 145	95 -- 101	-55.0636 kcal/mol	20.4542 kcal/mol	20.5091 kcal/mol	103 169@LB@ \| \|@LB@5'-GAG...CGACC CU U CA UUC CCUUAAA C A U G AUUCG...UGU-3'@LB@ GGUG GGAAGGG AG UUUCGC UGCCGU CUG GUAGACC GUU UAA CGCAA GGC UGACA@LB@ :\|\|\| ::\|\|\|\|\| \|\| \|:\|\|\|\| \|:\|\|\|\| \|\|\| \|:\|\|\|\|\| :\|\| :\|\| \|:\|\|\| ::\| ::\|\|\|@LB@ UCAC UUUUCCC UC AGAGCG AUGGCA GAC CGUCUGG UAA GUU GUGUU UUG GUUGU@LB@3'-UUU...GUCCU U A CCA UUAG U C U G U AGUGU...UCA-5'@LB@ \| \|@LB@ 132 70@LB@@LB@@LB@Energy -12.47 kcal/mol@LB@Hybridization Energy -55.0636 kcal/mol@LB@Unfolding Energy - mRNA 20.4542 kcal/mol@LB@Unfolding Energy - sRNA 20.5091 kcal/mol@LB@@LB@Position - mRNA 104 -- 168@LB@Position - sRNA 71 -- 131@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 95 -- 101@LB@
43	0.001574	0.1267	stm4453	treC	-17.28	0.050040	221 -- 269	37 -- 81	trehalose- 6-P hydrolase	_____________220____________________270\n_____________\|______________________\|\n5'-CGU...CGCAC_______AA_____________UAUUC...GCG-3'\n______________UUGCAGG__ACAACAAU__GAC\n______________\|\|\|\|\|::__\|\|\|\|\|\|\|:__:\|\|\n______________AACGUUU__UGUUGUUG__UUG\n3'-UUU...UGGUU_______G_________UG___UAGUG...UCA-5'\n_____________\|______________________\|\n_____________82_____________________36\n	196 -- 202	77 -- 83	-15.8671 kcal/mol	3.0955 kcal/mol	3.80821 kcal/mol	220 270@LB@ \| \|@LB@5'-CGU...CGCAC AA UAUUC...GCG-3'@LB@ UUGCAGG ACAACAAU GAC@LB@ \|\|\|\|\|:: \|\|\|\|\|\|\|: :\|\|@LB@ AACGUUU UGUUGUUG UUG@LB@3'-UUU...UGGUU G UG UAGUG...UCA-5'@LB@ \| \|@LB@ 82 36@LB@@LB@@LB@Energy -17.28 kcal/mol@LB@Hybridization Energy -15.8671 kcal/mol@LB@Unfolding Energy - mRNA 3.0955 kcal/mol@LB@Unfolding Energy - sRNA 3.80821 kcal/mol@LB@@LB@Position - mRNA 221 -- 269@LB@Position - sRNA 37 -- 81@LB@Position Seed - mRNA 196 -- 202@LB@Position Seed - sRNA 77 -- 83@LB@
90	0.004161	0.1596	stm1124	putA	-17.89	0.039481	159 -- 201	125 -- 168	trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase	_____________158______________________________________________202\n_____________\|________________________________________________\|\n5'-UAA...CGCUA____U___UUA________A_C____A_____________________UGGGA...UAA-3'\n______________ACUA_UGG___GCAGAUAG_G_GAAU__ACAGG_AGU___AGAGGGUA\n______________\|\|\|\|_\|\|\|___:\|\|\|\|:\|\|_\|_:\|\|\|__\|\|\|\|\|_\|\|\|___\|:\|\|\|\|\|\|\n______________UGAU_ACC___UGUCUGUC_C_UUUA__UGUCC_UCA___UUUCCCAU\n3'-UUU...AUUAG___________________C_A____CA_____U___CUU________CAGAG...UCA-5'\n_____________\|________________________________________________\|\n_____________169______________________________________________124\n	195 -- 201	125 -- 131	-33.9858 kcal/mol	7.26317 kcal/mol	8.4265 kcal/mol	158 202@LB@ \| \|@LB@5'-UAA...CGCUA U UUA A C A UGGGA...UAA-3'@LB@ ACUA UGG GCAGAUAG G GAAU ACAGG AGU AGAGGGUA@LB@ \|\|\|\| \|\|\| :\|\|\|\|:\|\| \| :\|\|\| \|\|\|\|\| \|\|\| \|:\|\|\|\|\|\|@LB@ UGAU ACC UGUCUGUC C UUUA UGUCC UCA UUUCCCAU@LB@3'-UUU...AUUAG C A CA U CUU CAGAG...UCA-5'@LB@ \| \|@LB@ 169 124@LB@@LB@@LB@Energy -17.89 kcal/mol@LB@Hybridization Energy -33.9858 kcal/mol@LB@Unfolding Energy - mRNA 7.26317 kcal/mol@LB@Unfolding Energy - sRNA 8.4265 kcal/mol@LB@@LB@Position - mRNA 159 -- 201@LB@Position - sRNA 125 -- 168@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 125 -- 131@LB@
96	0.004303	0.1596	stm4243	yjbN	-11.74	0.363864	272 -- 280	155 -- 163	tRNA dihydrouridine(20/20a) synthase DusA	_____________271______________________________281\n_____________\|________________________________\|\n5'-UUA...UUAAU___AC_________C____A_____CCU____GGCAG...GUU-3'\n______________GGG____UGUACGG_GAAG_UGAAA___GGGG\n______________\|\|\|____\|\|\|\|\|:\|_\|\|\|\|_\|\|\|\|\|___:\|\|\|\n______________CCC____ACAUGUC_CUUC_ACUUU___UCCC\n3'-UUU...UCUGU___AUUU__________________U______AUCAG...UCA-5'\n_____________\|________________________________\|\n_____________164______________________________154\n	58 -- 64	141 -- 147	-26.0653 kcal/mol	8.12251 kcal/mol	7.07058 kcal/mol	271 281@LB@ \| \|@LB@5'-UUA...UUAAU AC C A CCU GGCAG...GUU-3'@LB@ GGG UGUACGG GAAG UGAAA GGGG@LB@ \|\|\| \|\|\|\|\|:\| \|\|\|\| \|\|\|\|\| :\|\|\|@LB@ CCC ACAUGUC CUUC ACUUU UCCC@LB@3'-UUU...UCUGU AUUU U AUCAG...UCA-5'@LB@ \| \|@LB@ 164 154@LB@@LB@@LB@Energy -11.74 kcal/mol@LB@Hybridization Energy -26.0653 kcal/mol@LB@Unfolding Energy - mRNA 8.12251 kcal/mol@LB@Unfolding Energy - sRNA 7.07058 kcal/mol@LB@@LB@Position - mRNA 272 -- 280@LB@Position - sRNA 155 -- 163@LB@Position Seed - mRNA 58 -- 64@LB@Position Seed - sRNA 141 -- 147@LB@
14	0.0002233	0.05681	stm3843	trmE	-16.53	0.066478	207 -- 249	37 -- 85	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	_____________206______________250\n_____________\|________________\|\n5'-UGA...CAUGA________________UAUCG...CGC-3'\n______________GC___CAUAACGACAC\n______________\|\|___\|\|:\|\|\|:\|\|\|\|\n______________CG___GUGUUGUUGUG\n3'-UUU...GUUAA__UUU___________UUGUA...UCA-5'\n_____________\|________________\|\n_____________86_______________36\n	211 -- 217	75 -- 81	-15.3625 kcal/mol	2.22645 kcal/mol	3.72363 kcal/mol	206 250@LB@ \| \|@LB@5'-UGA...CAUGA UAUCG...CGC-3'@LB@ GC CAUAACGACAC@LB@ \|\| \|\|:\|\|\|:\|\|\|\|@LB@ CG GUGUUGUUGUG@LB@3'-UUU...GUUAA UUU UUGUA...UCA-5'@LB@ \| \|@LB@ 86 36@LB@@LB@@LB@Energy -16.53 kcal/mol@LB@Hybridization Energy -15.3625 kcal/mol@LB@Unfolding Energy - mRNA 2.22645 kcal/mol@LB@Unfolding Energy - sRNA 3.72363 kcal/mol@LB@@LB@Position - mRNA 207 -- 249@LB@Position - sRNA 37 -- 85@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 75 -- 81@LB@
117	0.005749	0.1738	stm2562	yfhA	-19.31	0.022783	16 -- 66	47 -- 100	two-component system response regulator GlrR	_____________15____________67\n_____________\|_____________\|\n5'-CUG...AGGCC_____________UCGAU...UCG-3'\n______________UUGCAAACGCAGC\n______________\|\|\|\|\|\|\|\|:\|\|:\|\n______________AACGUUUGUGUUG\n3'-UUU...UGGUU_____________UUGUG...UCA-5'\n_____________\|_____________\|\n_____________101___________46\n	28 -- 34	80 -- 86	-18.759 kcal/mol	3.6583 kcal/mol	3.5889 kcal/mol	15 67@LB@ \| \|@LB@5'-CUG...AGGCC UCGAU...UCG-3'@LB@ UUGCAAACGCAGC@LB@ \|\|\|\|\|\|\|\|:\|\|:\|@LB@ AACGUUUGUGUUG@LB@3'-UUU...UGGUU UUGUG...UCA-5'@LB@ \| \|@LB@ 101 46@LB@@LB@@LB@Energy -19.31 kcal/mol@LB@Hybridization Energy -18.759 kcal/mol@LB@Unfolding Energy - mRNA 3.6583 kcal/mol@LB@Unfolding Energy - sRNA 3.5889 kcal/mol@LB@@LB@Position - mRNA 16 -- 66@LB@Position - sRNA 47 -- 100@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 80 -- 86@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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