Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
44	0.000471	0.03766	b3624	waaZ	-7.10	0.250129	289 -- 295	1 -- 7	lipopolysaccharide KdoIII transferase; lipopolysaccharide core biosynthesis protein	_____________288_____296\n_____________\|_______\|\n5'-AGA...UUCGG_______AUCGC-3'\n______________GCCCUAC\n______________:\|\|\|\|\|\|\n______________UGGGAUG\n3'-AUU...AGACA_______-5'\n_____________\|______\|\n_____________8______1\n	289 -- 295	1 -- 7	-11.6335 kcal/mol	2.52016 kcal/mol	2.03949 kcal/mol	288 296@LB@ \| \|@LB@5'-AGA...UUCGG AUCGC-3'@LB@ GCCCUAC@LB@ :\|\|\|\|\|\|@LB@ UGGGAUG@LB@3'-AUU...AGACA -5'@LB@ \| \|@LB@ 8 1@LB@@LB@@LB@Energy -7.10 kcal/mol@LB@Hybridization Energy -11.6335 kcal/mol@LB@Unfolding Energy - mRNA 2.52016 kcal/mol@LB@Unfolding Energy - sRNA 2.03949 kcal/mol@LB@@LB@Position - mRNA 289 -- 295@LB@Position - sRNA 1 -- 7@LB@Position Seed - mRNA 289 -- 295@LB@Position Seed - sRNA 1 -- 7@LB@
138	0.004994	0.127	b3633	waaA	-9.37	0.068643	230 -- 259	48 -- 75	3-deoxy-D-manno-octulosonic-acid transferase (KDO transferase)	_____________229____________________________260\n_____________\|______________________________\|\n5'-UCG...CUUCU___CCU__________CU_______A____GCUCU...AAC-3'\n______________CUA___UAUUCAGCCG__GAUCUGG_UACG\n______________\|\|\|___\|\|\|\|\|\|\|\|\|:__:\|\|\|:\|:_\|\|\|\|\n______________GAU___AUAAGUCGGU__UUAGGCU_AUGC\n3'-AUU...CCGCC___UUU___________________A____ACUUC...AUG-5'\n_____________\|______________________________\|\n_____________76_____________________________47\n	248 -- 254	53 -- 59	-25.3567 kcal/mol	8.33931 kcal/mol	7.67232 kcal/mol	229 260@LB@ \| \|@LB@5'-UCG...CUUCU CCU CU A GCUCU...AAC-3'@LB@ CUA UAUUCAGCCG GAUCUGG UACG@LB@ \|\|\| \|\|\|\|\|\|\|\|\|: :\|\|\|:\|: \|\|\|\|@LB@ GAU AUAAGUCGGU UUAGGCU AUGC@LB@3'-AUU...CCGCC UUU A ACUUC...AUG-5'@LB@ \| \|@LB@ 76 47@LB@@LB@@LB@Energy -9.37 kcal/mol@LB@Hybridization Energy -25.3567 kcal/mol@LB@Unfolding Energy - mRNA 8.33931 kcal/mol@LB@Unfolding Energy - sRNA 7.67232 kcal/mol@LB@@LB@Position - mRNA 230 -- 259@LB@Position - sRNA 48 -- 75@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 53 -- 59@LB@
135	0.004796	0.125	b3634	coaD	-9.06	0.083023	69 -- 85	49 -- 66	pantetheine-phosphate adenylyltransferase	_____________68_________________86\n_____________\|__________________\|\n5'-GGA...UUUAC________CG________CGUAG...UUC-3'\n______________UCA_CCGA__CCGAUUAC\n______________\|\|\|_\|\|:\|__\|\|\|\|\|\|\|\|\n______________AGU_GGUU__GGCUAAUG\n3'-AUU...UUAUA___C____UA________CACUU...AUG-5'\n_____________\|__________________\|\n_____________67_________________48\n	79 -- 85	49 -- 55	-17.7938 kcal/mol	2.54845 kcal/mol	6.20469 kcal/mol	68 86@LB@ \| \|@LB@5'-GGA...UUUAC CG CGUAG...UUC-3'@LB@ UCA CCGA CCGAUUAC@LB@ \|\|\| \|\|:\| \|\|\|\|\|\|\|\|@LB@ AGU GGUU GGCUAAUG@LB@3'-AUU...UUAUA C UA CACUU...AUG-5'@LB@ \| \|@LB@ 67 48@LB@@LB@@LB@Energy -9.06 kcal/mol@LB@Hybridization Energy -17.7938 kcal/mol@LB@Unfolding Energy - mRNA 2.54845 kcal/mol@LB@Unfolding Energy - sRNA 6.20469 kcal/mol@LB@@LB@Position - mRNA 69 -- 85@LB@Position - sRNA 49 -- 66@LB@Position Seed - mRNA 79 -- 85@LB@Position Seed - sRNA 49 -- 55@LB@
173	0.007216	0.1466	b3650	spoT	-5.39	0.520602	11 -- 17	54 -- 60	bifunctional (p)ppGpp synthetase II/ guanosine-3' 5'-bis pyrophosphate 3'-pyrophosphohydrolase	_____________10______18\n_____________\|_______\|\n5'-UAG...GGAAA_______CUGGU...CUC-3'\n______________GGAUCCG\n______________::\|\|\|\|\|\n______________UUUAGGC\n3'-AUU...GUCGG_______UAAUG...AUG-5'\n_____________\|_______\|\n_____________61______53\n	11 -- 17	54 -- 60	-6.87427 kcal/mol	1.21245 kcal/mol	0.286889 kcal/mol	10 18@LB@ \| \|@LB@5'-UAG...GGAAA CUGGU...CUC-3'@LB@ GGAUCCG@LB@ ::\|\|\|\|\|@LB@ UUUAGGC@LB@3'-AUU...GUCGG UAAUG...AUG-5'@LB@ \| \|@LB@ 61 53@LB@@LB@@LB@Energy -5.39 kcal/mol@LB@Hybridization Energy -6.87427 kcal/mol@LB@Unfolding Energy - mRNA 1.21245 kcal/mol@LB@Unfolding Energy - sRNA 0.286889 kcal/mol@LB@@LB@Position - mRNA 11 -- 17@LB@Position - sRNA 54 -- 60@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 54 -- 60@LB@
56	0.0008344	0.05242	b3651	trmH	-7.19	0.238334	68 -- 84	17 -- 32	tRNA mG18-2'-O-methyltransferase SAM-dependent	_____________67________________85\n_____________\|_________________\|\n5'-CGC...GAAGA_________UC______ACAGC...UUU-3'\n______________GAAAGAUGG__GCGUCU\n______________\|\|\|\|\|\|\|:\|__:\|:\|\|\|\n______________CUUUCUAUC__UGUAGA\n3'-AUU...CCAGA_________U_______AUGGA...AUG-5'\n_____________\|_________________\|\n_____________33________________16\n	70 -- 76	24 -- 30	-18.3937 kcal/mol	6.9506 kcal/mol	4.26839 kcal/mol	67 85@LB@ \| \|@LB@5'-CGC...GAAGA UC ACAGC...UUU-3'@LB@ GAAAGAUGG GCGUCU@LB@ \|\|\|\|\|\|\|:\| :\|:\|\|\|@LB@ CUUUCUAUC UGUAGA@LB@3'-AUU...CCAGA U AUGGA...AUG-5'@LB@ \| \|@LB@ 33 16@LB@@LB@@LB@Energy -7.19 kcal/mol@LB@Hybridization Energy -18.3937 kcal/mol@LB@Unfolding Energy - mRNA 6.9506 kcal/mol@LB@Unfolding Energy - sRNA 4.26839 kcal/mol@LB@@LB@Position - mRNA 68 -- 84@LB@Position - sRNA 17 -- 32@LB@Position Seed - mRNA 70 -- 76@LB@Position Seed - sRNA 24 -- 30@LB@
39	0.0004396	0.03766	b3669	uhpA	-8.50	0.116685	77 -- 96	47 -- 66	response regulator in two-component regulatory system wtih UhpB	_____________76___________________97\n_____________\|____________________\|\n5'-UCA...CGUAA______C_______CG____UUAAC...UUG-3'\n______________UCAGUC_GAUCCGA__AUGU\n______________\|\|\|\|:\|_:\|\|\|\|\|\|__\|:\|\|\n______________AGUCGG_UUAGGCU__UGCA\n3'-AUU...UUAUA______U_______AA____CUUCA...AUG-5'\n_____________\|____________________\|\n_____________67___________________46\n	84 -- 90	53 -- 59	-21.6061 kcal/mol	5.63687 kcal/mol	7.4916 kcal/mol	76 97@LB@ \| \|@LB@5'-UCA...CGUAA C CG UUAAC...UUG-3'@LB@ UCAGUC GAUCCGA AUGU@LB@ \|\|\|\|:\| :\|\|\|\|\|\| \|:\|\|@LB@ AGUCGG UUAGGCU UGCA@LB@3'-AUU...UUAUA U AA CUUCA...AUG-5'@LB@ \| \|@LB@ 67 46@LB@@LB@@LB@Energy -8.50 kcal/mol@LB@Hybridization Energy -21.6061 kcal/mol@LB@Unfolding Energy - mRNA 5.63687 kcal/mol@LB@Unfolding Energy - sRNA 7.4916 kcal/mol@LB@@LB@Position - mRNA 77 -- 96@LB@Position - sRNA 47 -- 66@LB@Position Seed - mRNA 84 -- 90@LB@Position Seed - sRNA 53 -- 59@LB@
17	5.719e-05	0.01183	b3675	yidG	-12.05	0.010096	82 -- 130	1 -- 56	inner membrane protein	_____________81_____________________________________________________________131\n_____________\|______________________________________________________________\|\n5'-CGC...UUGCG_________CAAU_A_____CGAUGC__________GG________________________ACCAA...CGU-3'\n______________CUGGUUACG____G_AAAAG______GUCUGAAAGA___________ACCU____GCCCUAC\n______________\|:\|:\|\|\|\|\|____\|_\|\|\|\|\|______\|\|\|\|\|\|\|\|\|\|___________\|\|\|\|____:\|\|\|\|\|\|\n______________GGCUAAUGC____C_UUUUC______CAGACUUUCU___________UGGA____UGGGAUG\n3'-AUU...GUUUA_________ACUU_A_____________________AUCUUGUAGAA____GACA_______-5'\n_____________\|_____________________________________________________________\|\n_____________57____________________________________________________________1\n	124 -- 130	1 -- 7	-38.348 kcal/mol	13.6157 kcal/mol	12.7022 kcal/mol	81 131@LB@ \| \|@LB@5'-CGC...UUGCG CAAU A CGAUGC GG ACCAA...CGU-3'@LB@ CUGGUUACG G AAAAG GUCUGAAAGA ACCU GCCCUAC@LB@ \|:\|:\|\|\|\|\| \| \|\|\|\|\| \|\|\|\|\|\|\|\|\|\| \|\|\|\| :\|\|\|\|\|\|@LB@ GGCUAAUGC C UUUUC CAGACUUUCU UGGA UGGGAUG@LB@3'-AUU...GUUUA ACUU A AUCUUGUAGAA GACA -5'@LB@ \| \|@LB@ 57 1@LB@@LB@@LB@Energy -12.05 kcal/mol@LB@Hybridization Energy -38.348 kcal/mol@LB@Unfolding Energy - mRNA 13.6157 kcal/mol@LB@Unfolding Energy - sRNA 12.7022 kcal/mol@LB@@LB@Position - mRNA 82 -- 130@LB@Position - sRNA 1 -- 56@LB@Position Seed - mRNA 124 -- 130@LB@Position Seed - sRNA 1 -- 7@LB@
41	0.000458	0.03766	b3679	yidK	-16.91	0.000078	119 -- 159	1 -- 60	putative transporter	_____________118__________________________________________________________160\n_____________\|____________________________________________________________\|\n5'-UUU...UUUAA________C_____________________CCCGAC________________________AUUCA...AAG-3'\n______________GAAUCUGA_UGC______________CUGA________GCAUUUUACCUCU___CCCUAU\n______________:\|\|\|\|:\|\|_\|:\|______________\|\|\|\|________:\|\|\|:\|\|\|\|\|\|\|\|___\|\|\|\|\|:\n______________UUUAGGCU_AUG______________GACU________UGUAGAAUGGAGA___GGGAUG\n3'-AUU...GUCGG________A___CACUUCAUUUUCCA____UUCUAUCU_____________CAU______-5'\n_____________\|___________________________________________________________\|\n_____________61__________________________________________________________1\n	147 -- 153	10 -- 16	-34.139 kcal/mol	4.55251 kcal/mol	12.7042 kcal/mol	118 160@LB@ \| \|@LB@5'-UUU...UUUAA C CCCGAC AUUCA...AAG-3'@LB@ GAAUCUGA UGC CUGA GCAUUUUACCUCU CCCUAU@LB@ :\|\|\|\|:\|\| \|:\| \|\|\|\| :\|\|\|:\|\|\|\|\|\|\|\| \|\|\|\|\|:@LB@ UUUAGGCU AUG GACU UGUAGAAUGGAGA GGGAUG@LB@3'-AUU...GUCGG A CACUUCAUUUUCCA UUCUAUCU CAU -5'@LB@ \| \|@LB@ 61 1@LB@@LB@@LB@Energy -16.91 kcal/mol@LB@Hybridization Energy -34.139 kcal/mol@LB@Unfolding Energy - mRNA 4.55251 kcal/mol@LB@Unfolding Energy - sRNA 12.7042 kcal/mol@LB@@LB@Position - mRNA 119 -- 159@LB@Position - sRNA 1 -- 60@LB@Position Seed - mRNA 147 -- 153@LB@Position Seed - sRNA 10 -- 16@LB@
128	0.004347	0.1195	b3690	cbrA	-9.56	0.060736	197 -- 211	17 -- 31	colicin M resistance protein; FAD-binding protein putative oxidoreductase	_____________196_____________212\n_____________\|_______________\|\n5'-CAG...AGUGA_______________CGACG...AAA-3'\n______________AAAGAUGGAACAUUU\n______________\|\|\|\|\|\|:\|\|\|\|\|\|:\|\n______________UUUCUAUCUUGUAGA\n3'-AUU...CAGAC_______________AUGGA...AUG-5'\n_____________\|_______________\|\n_____________32______________16\n	204 -- 210	18 -- 24	-17.0326 kcal/mol	3.22818 kcal/mol	4.26586 kcal/mol	196 212@LB@ \| \|@LB@5'-CAG...AGUGA CGACG...AAA-3'@LB@ AAAGAUGGAACAUUU@LB@ \|\|\|\|\|\|:\|\|\|\|\|\|:\|@LB@ UUUCUAUCUUGUAGA@LB@3'-AUU...CAGAC AUGGA...AUG-5'@LB@ \| \|@LB@ 32 16@LB@@LB@@LB@Energy -9.56 kcal/mol@LB@Hybridization Energy -17.0326 kcal/mol@LB@Unfolding Energy - mRNA 3.22818 kcal/mol@LB@Unfolding Energy - sRNA 4.26586 kcal/mol@LB@@LB@Position - mRNA 197 -- 211@LB@Position - sRNA 17 -- 31@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 18 -- 24@LB@
72	0.001216	0.05856	b3699	gyrB	-10.53	0.031588	1 -- 17	9 -- 25	DNA gyrase subunit B	______________1________________18\n______________\|________________\|\n___________5'-___UG____________AGCUU...GCA-3'\n______________AGA__AUUUUGCCUCUG\n______________\|\|\|__\|\|:\|\|:\|\|\|\|\|\|\n______________UCU__UAGAAUGGAGAC\n3'-AUU...UUCUA___UG____________AUGGGAUG-5'\n_____________\|_________________\|\n_____________26________________8\n	11 -- 17	9 -- 15	-19.3204 kcal/mol	1.07855 kcal/mol	7.71376 kcal/mol	1 18@LB@ \| \|@LB@ 5'- UG AGCUU...GCA-3'@LB@ AGA AUUUUGCCUCUG@LB@ \|\|\| \|\|:\|\|:\|\|\|\|\|\|@LB@ UCU UAGAAUGGAGAC@LB@3'-AUU...UUCUA UG AUGGGAUG-5'@LB@ \| \|@LB@ 26 8@LB@@LB@@LB@Energy -10.53 kcal/mol@LB@Hybridization Energy -19.3204 kcal/mol@LB@Unfolding Energy - mRNA 1.07855 kcal/mol@LB@Unfolding Energy - sRNA 7.71376 kcal/mol@LB@@LB@Position - mRNA 1 -- 17@LB@Position - sRNA 9 -- 25@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 9 -- 15@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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