Input and runtime details for job 3202356 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3202356 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:02 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:03:47 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:03:55 CET 2018
	Job Completed		@ Sat Feb 17 02:04:28 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3202356&toolName=CopraRNA ( 30 days expiry )

Description of the job

GcvB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
106	0.005253	0.1728	stm1172	flgM	-15.84	0.086212	196 -- 254	36 -- 82	anti-sigma-28 factor FlgM	_____________195________________255\n_____________\|__________________\|\n5'-GGC...GACGC__G_______________CCGGU...CGU-3'\n______________GC_AAAC_CAGCGACACG\n______________\|\|_\|\|\|\|_\|\|:\|:\|\|\|\|:\n______________CG_UUUG_GUUGUUGUGU\n3'-UUU...GUUAA_______U__________UGUAG...UCA-5'\n_____________\|__________________\|\n_____________83_________________35\n	263 -- 269	74 -- 80	-18.0556 kcal/mol	5.48392 kcal/mol	3.73436 kcal/mol	195 255@LB@ \| \|@LB@5'-GGC...GACGC G CCGGU...CGU-3'@LB@ GC AAAC CAGCGACACG@LB@ \|\| \|\|\|\| \|\|:\|:\|\|\|\|:@LB@ CG UUUG GUUGUUGUGU@LB@3'-UUU...GUUAA U UGUAG...UCA-5'@LB@ \| \|@LB@ 83 35@LB@@LB@@LB@Energy -15.84 kcal/mol@LB@Hybridization Energy -18.0556 kcal/mol@LB@Unfolding Energy - mRNA 5.48392 kcal/mol@LB@Unfolding Energy - sRNA 3.73436 kcal/mol@LB@@LB@Position - mRNA 196 -- 254@LB@Position - sRNA 36 -- 82@LB@Position Seed - mRNA 263 -- 269@LB@Position Seed - sRNA 74 -- 80@LB@
137	0.006825	0.1774	stm1196	acpP	-14.14	0.161333	197 -- 223	36 -- 60	acyl carrier protein	_____________196___________________________________________224\n_____________\|_____________________________________________\|\n5'-ACU...AGAAC_______GAAA_____C____A____CU__GCGUUAA________AAGUA...UGG-3'\n______________GCGUUAA____AUUAU_GGCG_ACAG__GG_______GCAGGAAG\n______________:\|:\|\|\|\|____\|:\|\|\|_\|:\|:_\|\|\|\|__\|\|_______:\|\|\|\|\|\|\|\n______________UGUAAUU____UGAUA_CUGU_UGUC__CC_______UGUCCUUC\n3'-UUU...CACGA_______AG_______C____C________AUUUACA________ACUUU...UCA-5'\n_____________\|_____________________________________________\|\n_____________61____________________________________________35\n	258 -- 264	137 -- 143	-28.6095 kcal/mol	10.3633 kcal/mol	7.13805 kcal/mol	196 224@LB@ \| \|@LB@5'-ACU...AGAAC GAAA C A CU GCGUUAA AAGUA...UGG-3'@LB@ GCGUUAA AUUAU GGCG ACAG GG GCAGGAAG@LB@ :\|:\|\|\|\| \|:\|\|\| \|:\|: \|\|\|\| \|\| :\|\|\|\|\|\|\|@LB@ UGUAAUU UGAUA CUGU UGUC CC UGUCCUUC@LB@3'-UUU...CACGA AG C C AUUUACA ACUUU...UCA-5'@LB@ \| \|@LB@ 61 35@LB@@LB@@LB@Energy -14.14 kcal/mol@LB@Hybridization Energy -28.6095 kcal/mol@LB@Unfolding Energy - mRNA 10.3633 kcal/mol@LB@Unfolding Energy - sRNA 7.13805 kcal/mol@LB@@LB@Position - mRNA 197 -- 223@LB@Position - sRNA 36 -- 60@LB@Position Seed - mRNA 258 -- 264@LB@Position Seed - sRNA 137 -- 143@LB@
29	0.0007392	0.08814	stm3096	yqgE	-16.45	0.068552	198 -- 251	37 -- 84	DUF179 domain-containing protein	_____________197___________252\n_____________\|_____________\|\n5'-UGG...UAUAU_____________CAGGA...GCG-3'\n______________UUGCGAGCACAAC\n______________\|\|\|\|:\|:\|\|\|\|\|\|\n______________AACGUUUGUGUUG\n3'-UUU...UGGUU_____________UUGUG...UCA-5'\n_____________\|_____________\|\n_____________85____________36\n	284 -- 290	80 -- 86	-18.5161 kcal/mol	2.82597 kcal/mol	3.5889 kcal/mol	197 252@LB@ \| \|@LB@5'-UGG...UAUAU CAGGA...GCG-3'@LB@ UUGCGAGCACAAC@LB@ \|\|\|\|:\|:\|\|\|\|\|\|@LB@ AACGUUUGUGUUG@LB@3'-UUU...UGGUU UUGUG...UCA-5'@LB@ \| \|@LB@ 85 36@LB@@LB@@LB@Energy -16.45 kcal/mol@LB@Hybridization Energy -18.5161 kcal/mol@LB@Unfolding Energy - mRNA 2.82597 kcal/mol@LB@Unfolding Energy - sRNA 3.5889 kcal/mol@LB@@LB@Position - mRNA 198 -- 251@LB@Position - sRNA 37 -- 84@LB@Position Seed - mRNA 284 -- 290@LB@Position Seed - sRNA 80 -- 86@LB@
157	0.008234	0.1845	stm0693	fur	-16.04	0.080078	199 -- 253	31 -- 90	ferric iron uptake transcriptional regulator	_____________198________________________254\n_____________\|__________________________\|\n5'-GCA...AUUCC___U___UG__________C______AAGAA...ACG-3'\n______________GCA_GAC__ACAACAAUAC_GCAUUA\n______________\|\|\|_:\|\|__\|\|\|\|\|\|\|:\|\|_:\|\|\|:\|\n______________CGU_UUG__UGUUGUUGUG_UGUAGU\n3'-UUU...GUUAA___________________U______GUUGG...UCA-5'\n_____________\|__________________________\|\n_____________91_________________________30\n	211 -- 217	75 -- 81	-20.9388 kcal/mol	2.89423 kcal/mol	5.29697 kcal/mol	198 254@LB@ \| \|@LB@5'-GCA...AUUCC U UG C AAGAA...ACG-3'@LB@ GCA GAC ACAACAAUAC GCAUUA@LB@ \|\|\| :\|\| \|\|\|\|\|\|\|:\|\| :\|\|\|:\|@LB@ CGU UUG UGUUGUUGUG UGUAGU@LB@3'-UUU...GUUAA U GUUGG...UCA-5'@LB@ \| \|@LB@ 91 30@LB@@LB@@LB@Energy -16.04 kcal/mol@LB@Hybridization Energy -20.9388 kcal/mol@LB@Unfolding Energy - mRNA 2.89423 kcal/mol@LB@Unfolding Energy - sRNA 5.29697 kcal/mol@LB@@LB@Position - mRNA 199 -- 253@LB@Position - sRNA 31 -- 90@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 75 -- 81@LB@
67	0.003193	0.1596	stm0122	ftsI	-11.87	0.349270	199 -- 241	52 -- 90	peptidoglycan glycosyltransferase FtsI	_____________198_______________________242\n_____________\|_________________________\|\n5'-GUU...CGCGA____UUG_____UG___________CUGAU...UGG-3'\n______________UGCA___GACAU__AAUGGCGCAAC\n______________\|\|\|\|___:\|\|\|:__\|\|:::\|:\|\|\|\|\n______________ACGU___UUGUG__UUGUUGUGUUG\n3'-UUU...GGUUA_________________________UAGUG...UCA-5'\n_____________\|_________________________\|\n_____________91________________________51\n	62 -- 68	72 -- 78	-17.673 kcal/mol	4.85802 kcal/mol	3.80548 kcal/mol	198 242@LB@ \| \|@LB@5'-GUU...CGCGA UUG UG CUGAU...UGG-3'@LB@ UGCA GACAU AAUGGCGCAAC@LB@ \|\|\|\| :\|\|\|: \|\|:::\|:\|\|\|\|@LB@ ACGU UUGUG UUGUUGUGUUG@LB@3'-UUU...GGUUA UAGUG...UCA-5'@LB@ \| \|@LB@ 91 51@LB@@LB@@LB@Energy -11.87 kcal/mol@LB@Hybridization Energy -17.673 kcal/mol@LB@Unfolding Energy - mRNA 4.85802 kcal/mol@LB@Unfolding Energy - sRNA 3.80548 kcal/mol@LB@@LB@Position - mRNA 199 -- 241@LB@Position - sRNA 52 -- 90@LB@Position Seed - mRNA 62 -- 68@LB@Position Seed - sRNA 72 -- 78@LB@
21	0.000482	0.08173	stm1057	pepN	-18.92	0.026522	201 -- 244	36 -- 81	aminopeptidase N	_____________200______________245\n_____________\|________________\|\n5'-CUU...UUUAU___________C____GCCAA...AAA-3'\n______________GACACAACAGC_ACAA\n______________:\|\|\|\|\|\|\|\|:\|_\|\|\|\|\n______________UUGUGUUGUUG_UGUU\n3'-UUU...AACGU________________GUAGU...UCA-5'\n_____________\|________________\|\n_____________82_______________35\n	207 -- 213	77 -- 83	-17.6161 kcal/mol	1.99149 kcal/mol	3.56182 kcal/mol	200 245@LB@ \| \|@LB@5'-CUU...UUUAU C GCCAA...AAA-3'@LB@ GACACAACAGC ACAA@LB@ :\|\|\|\|\|\|\|\|:\| \|\|\|\|@LB@ UUGUGUUGUUG UGUU@LB@3'-UUU...AACGU GUAGU...UCA-5'@LB@ \| \|@LB@ 82 35@LB@@LB@@LB@Energy -18.92 kcal/mol@LB@Hybridization Energy -17.6161 kcal/mol@LB@Unfolding Energy - mRNA 1.99149 kcal/mol@LB@Unfolding Energy - sRNA 3.56182 kcal/mol@LB@@LB@Position - mRNA 201 -- 244@LB@Position - sRNA 36 -- 81@LB@Position Seed - mRNA 207 -- 213@LB@Position Seed - sRNA 77 -- 83@LB@
8	9.263e-05	0.04123	stm0369	prpC	-29.42	0.000431	202 -- 269	31 -- 91	2-methylcitrate synthase	_____________201______________________________________________________________270\n_____________\|________________________________________________________________\|\n5'-AAC...CAAUA__A_____________C_U___AA_____A__CAU____UU______AAAAA_____U______UUUCC...GUA-3'\n______________UG_CAGACACGACGAU_C_GCA__ACAAC_CG___GUCA__AAGCCU_____AUCGG__CGCGC\n______________\|\|_\|\|:\|\|\|\|:\|\|:\|:_\|_:\|\|__\|\|\|\|\|_\|\|___\|:\|\|__\|\|\|\|\|:_____\|\|\|:\|__\|:\|\|\|\n______________AC_GUUUGUGUUGUUG_G_UGU__UGUUG_GC___CGGU__UUCGGG_____UAGUC__GUGCG\n3'-UUU...GGUUA________________U_U___AG________AUU____U_________________UU_____UAAGG...UCA-5'\n_____________\|________________________________________________________________\|\n_____________92_______________________________________________________________30\n	211 -- 217	77 -- 83	-51.7305 kcal/mol	8.34164 kcal/mol	22.503 kcal/mol	201 270@LB@ \| \|@LB@5'-AAC...CAAUA A C U AA A CAU UU AAAAA U UUUCC...GUA-3'@LB@ UG CAGACACGACGAU C GCA ACAAC CG GUCA AAGCCU AUCGG CGCGC@LB@ \|\| \|\|:\|\|\|\|:\|\|:\|: \| :\|\| \|\|\|\|\| \|\| \|:\|\| \|\|\|\|\|: \|\|\|:\| \|:\|\|\|@LB@ AC GUUUGUGUUGUUG G UGU UGUUG GC CGGU UUCGGG UAGUC GUGCG@LB@3'-UUU...GGUUA U U AG AUU U UU UAAGG...UCA-5'@LB@ \| \|@LB@ 92 30@LB@@LB@@LB@Energy -29.42 kcal/mol@LB@Hybridization Energy -51.7305 kcal/mol@LB@Unfolding Energy - mRNA 8.34164 kcal/mol@LB@Unfolding Energy - sRNA 22.503 kcal/mol@LB@@LB@Position - mRNA 202 -- 269@LB@Position - sRNA 31 -- 91@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 77 -- 83@LB@
168	0.009144	0.1938	stm3609	yhjE	-18.08	0.036777	202 -- 261	35 -- 91	MFS transporter	_____________201__________________________________262\n_____________\|____________________________________\|\n5'-UAA...UAGUA_______A__C_____________U_____A_____AGGAA...UUG-3'\n______________UGCAAGC_AC_GC__CACAACA_C_CGACC_UGAGC\n______________\|\|\|\|\|:\|_\|\|_:\|__\|\|\|\|\|\|\|_\|_\|:\|\|\|_\|:\|\|\|\n______________ACGUUUG_UG_UG__GUGUUGU_G_GUUGG_AUUCG\n3'-UUU...GGUUA__________U__UU_______A_U_____C_____GUUUU...UCA-5'\n_____________\|____________________________________\|\n_____________92___________________________________34\n	215 -- 221	71 -- 77	-32.2191 kcal/mol	4.27941 kcal/mol	11.5198 kcal/mol	201 262@LB@ \| \|@LB@5'-UAA...UAGUA A C U A AGGAA...UUG-3'@LB@ UGCAAGC AC GC CACAACA C CGACC UGAGC@LB@ \|\|\|\|\|:\| \|\| :\| \|\|\|\|\|\|\| \| \|:\|\|\| \|:\|\|\|@LB@ ACGUUUG UG UG GUGUUGU G GUUGG AUUCG@LB@3'-UUU...GGUUA U UU A U C GUUUU...UCA-5'@LB@ \| \|@LB@ 92 34@LB@@LB@@LB@Energy -18.08 kcal/mol@LB@Hybridization Energy -32.2191 kcal/mol@LB@Unfolding Energy - mRNA 4.27941 kcal/mol@LB@Unfolding Energy - sRNA 11.5198 kcal/mol@LB@@LB@Position - mRNA 202 -- 261@LB@Position - sRNA 35 -- 91@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 71 -- 77@LB@
79	0.003723	0.1596	stm1723	trpE	-16.56	0.065817	202 -- 256	41 -- 91	anthranilate synthase subunit I	_____________201__________________257\n_____________\|____________________\|\n5'-UGA...AAAUG__________UU________CGGUU...CGG-3'\n______________GCAGCGACAU__GCAUUACA\n______________:\|\|:\|:\|\|\|:__:\|\|\|:\|\|\|\n______________UGUUGUUGUG__UGUAGUGU\n3'-UUU...GUUUG__________U_________UGGCA...UCA-5'\n_____________\|____________________\|\n_____________92___________________40\n	78 -- 84	66 -- 72	-20.8013 kcal/mol	5.41524 kcal/mol	5.89278 kcal/mol	201 257@LB@ \| \|@LB@5'-UGA...AAAUG UU CGGUU...CGG-3'@LB@ GCAGCGACAU GCAUUACA@LB@ :\|\|:\|:\|\|\|: :\|\|\|:\|\|\|@LB@ UGUUGUUGUG UGUAGUGU@LB@3'-UUU...GUUUG U UGGCA...UCA-5'@LB@ \| \|@LB@ 92 40@LB@@LB@@LB@Energy -16.56 kcal/mol@LB@Hybridization Energy -20.8013 kcal/mol@LB@Unfolding Energy - mRNA 5.41524 kcal/mol@LB@Unfolding Energy - sRNA 5.89278 kcal/mol@LB@@LB@Position - mRNA 202 -- 256@LB@Position - sRNA 41 -- 91@LB@Position Seed - mRNA 78 -- 84@LB@Position Seed - sRNA 66 -- 72@LB@
99	0.004942	0.1728	stm0604	ybdM	-18.77	0.028138	203 -- 249	36 -- 81	similar to E. coli orf hypothetical protein (AAC73702.1); Blastp hit to AAC73702.1 (209 aa) 77% identity in aa 5 - 209	_____________202___________________250\n_____________\|_____________________\|\n5'-AAA...CUUAU______C______________AAAUA...AUA-3'\n______________GCAACA_CGAC_UCACAACCG\n______________:\|\|\|\|\|_\|:\|\|_\|\|\|\|\|\|\|\|\|\n______________UGUUGU_GUUG_AGUGUUGGC\n3'-UUU...UGUGU___________U_________AUUCG...UCA-5'\n_____________\|_____________________\|\n_____________82____________________35\n	216 -- 222	62 -- 68	-25.0091 kcal/mol	1.37283 kcal/mol	7.72388 kcal/mol	202 250@LB@ \| \|@LB@5'-AAA...CUUAU C AAAUA...AUA-3'@LB@ GCAACA CGAC UCACAACCG@LB@ :\|\|\|\|\| \|:\|\| \|\|\|\|\|\|\|\|\|@LB@ UGUUGU GUUG AGUGUUGGC@LB@3'-UUU...UGUGU U AUUCG...UCA-5'@LB@ \| \|@LB@ 82 35@LB@@LB@@LB@Energy -18.77 kcal/mol@LB@Hybridization Energy -25.0091 kcal/mol@LB@Unfolding Energy - mRNA 1.37283 kcal/mol@LB@Unfolding Energy - sRNA 7.72388 kcal/mol@LB@@LB@Position - mRNA 203 -- 249@LB@Position - sRNA 36 -- 81@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 62 -- 68@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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