Input and runtime details for job 5882223 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5882223 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:29:59 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:39:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 03:02:39 CET 2018
	Post-Processing Finished		@ Sat Feb 17 03:02:47 CET 2018
	Job Completed		@ Sat Feb 17 03:03:15 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5882223&toolName=CopraRNA ( 30 days expiry )

Description of the job

Spot42

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
55	0.0008105	0.05184	b3262	yhdJ	-10.49	0.032282	53 -- 83	1 -- 34	DNA adenine methyltransferase SAM-dependent	_____________52_________________________________84\n_____________\|__________________________________\|\n5'-CCU...CUGCG____U____________A____GUG____G____GUAAA...GUG-3'\n______________CUGA__GAU_GGGCAUC__ACC___GUAC_CUGC\n______________\|\|\|\|__\|\|\|_\|::\|\|\|\|__\|\|\|___\|\|\|\|_\|\|:\|\n______________GACU__CUA_CUUGUAG__UGG___CAUG_GAUG\n3'-AUU...UUCCA____UU___U_______AA___AGA____G____-5'\n_____________\|_________________________________\|\n_____________35________________________________1\n	61 -- 67	18 -- 24	-29.8286 kcal/mol	10.2122 kcal/mol	9.14469 kcal/mol	52 84@LB@ \| \|@LB@5'-CCU...CUGCG U A GUG G GUAAA...GUG-3'@LB@ CUGA GAU GGGCAUC ACC GUAC CUGC@LB@ \|\|\|\| \|\|\| \|::\|\|\|\| \|\|\| \|\|\|\| \|\|:\|@LB@ GACU CUA CUUGUAG UGG CAUG GAUG@LB@3'-AUU...UUCCA UU U AA AGA G -5'@LB@ \| \|@LB@ 35 1@LB@@LB@@LB@Energy -10.49 kcal/mol@LB@Hybridization Energy -29.8286 kcal/mol@LB@Unfolding Energy - mRNA 10.2122 kcal/mol@LB@Unfolding Energy - sRNA 9.14469 kcal/mol@LB@@LB@Position - mRNA 53 -- 83@LB@Position - sRNA 1 -- 34@LB@Position Seed - mRNA 61 -- 67@LB@Position Seed - sRNA 18 -- 24@LB@
57	0.0008523	0.0526	b3281	aroE	-7.63	0.189523	225 -- 232	52 -- 59	dehydroshikimate reductase NAD(P)-binding	_____________224______233\n_____________\|________\|\n5'-UUU...UUGGU________AGCCC...AUG-3'\n______________AAUCCGAU\n______________\|\|\|\|\|\|\|\|\n______________UUAGGCUA\n3'-AUU...UCGGU________AUGCA...AUG-5'\n_____________\|________\|\n_____________60_______51\n	226 -- 232	52 -- 58	-9.52512 kcal/mol	1.61129 kcal/mol	0.296943 kcal/mol	224 233@LB@ \| \|@LB@5'-UUU...UUGGU AGCCC...AUG-3'@LB@ AAUCCGAU@LB@ \|\|\|\|\|\|\|\|@LB@ UUAGGCUA@LB@3'-AUU...UCGGU AUGCA...AUG-5'@LB@ \| \|@LB@ 60 51@LB@@LB@@LB@Energy -7.63 kcal/mol@LB@Hybridization Energy -9.52512 kcal/mol@LB@Unfolding Energy - mRNA 1.61129 kcal/mol@LB@Unfolding Energy - sRNA 0.296943 kcal/mol@LB@@LB@Position - mRNA 225 -- 232@LB@Position - sRNA 52 -- 59@LB@Position Seed - mRNA 226 -- 232@LB@Position Seed - sRNA 52 -- 58@LB@
40	0.0004513	0.03766	b3291	mscL	-6.99	0.263134	48 -- 58	55 -- 65	mechanosensitive channel protein high conductance	_____________47__________59\n_____________\|___________\|\n5'-UGU...UCUUC________C__UUUCU...UCU-3'\n______________CAGCCAAG_CC\n______________\|\|\|\|\|\|\|:_\|\|\n______________GUCGGUUU_GG\n3'-AUU...UAUAA________A__CUAAU...AUG-5'\n_____________\|___________\|\n_____________66__________54\n	49 -- 55	58 -- 64	-14.9389 kcal/mol	1.90813 kcal/mol	6.05961 kcal/mol	47 59@LB@ \| \|@LB@5'-UGU...UCUUC C UUUCU...UCU-3'@LB@ CAGCCAAG CC@LB@ \|\|\|\|\|\|\|: \|\|@LB@ GUCGGUUU GG@LB@3'-AUU...UAUAA A CUAAU...AUG-5'@LB@ \| \|@LB@ 66 54@LB@@LB@@LB@Energy -6.99 kcal/mol@LB@Hybridization Energy -14.9389 kcal/mol@LB@Unfolding Energy - mRNA 1.90813 kcal/mol@LB@Unfolding Energy - sRNA 6.05961 kcal/mol@LB@@LB@Position - mRNA 48 -- 58@LB@Position - sRNA 55 -- 65@LB@Position Seed - mRNA 49 -- 55@LB@Position Seed - sRNA 58 -- 64@LB@
81	0.001761	0.07647	b3311	rpsQ	-9.62	0.058411	217 -- 225	1 -- 9	30S ribosomal subunit protein S17	_____________216_______226\n_____________\|_________\|\n5'-GGU...AAUCC_________AAGGU...UCU-3'\n______________GUACUCUGC\n______________\|\|\|\|:\|\|:\|\n______________CAUGGGAUG\n3'-AUU...GGAGA_________-5'\n_____________\|________\|\n_____________10_______1\n	219 -- 225	1 -- 7	-13.7932 kcal/mol	2.07071 kcal/mol	2.12872 kcal/mol	216 226@LB@ \| \|@LB@5'-GGU...AAUCC AAGGU...UCU-3'@LB@ GUACUCUGC@LB@ \|\|\|\|:\|\|:\|@LB@ CAUGGGAUG@LB@3'-AUU...GGAGA -5'@LB@ \| \|@LB@ 10 1@LB@@LB@@LB@Energy -9.62 kcal/mol@LB@Hybridization Energy -13.7932 kcal/mol@LB@Unfolding Energy - mRNA 2.07071 kcal/mol@LB@Unfolding Energy - sRNA 2.12872 kcal/mol@LB@@LB@Position - mRNA 217 -- 225@LB@Position - sRNA 1 -- 9@LB@Position Seed - mRNA 219 -- 225@LB@Position Seed - sRNA 1 -- 7@LB@
195	0.008707	0.1558	b3318	rplW	-7.58	0.194751	202 -- 213	43 -- 55	50S ribosomal subunit protein L23	_____________201___________214\n_____________\|_____________\|\n5'-GCA...UGGCA_____________AACGU...AAG-3'\n______________UGAUU_CGUGAAG\n______________:\|\|\|\|_\|\|\|\|\|\|\|\n______________GCUAA_GCACUUC\n3'-AUU...UUUAG_____U_______AUUUU...AUG-5'\n_____________\|_____________\|\n_____________56____________42\n	207 -- 213	43 -- 49	-13.4464 kcal/mol	2.60751 kcal/mol	3.2693 kcal/mol	201 214@LB@ \| \|@LB@5'-GCA...UGGCA AACGU...AAG-3'@LB@ UGAUU CGUGAAG@LB@ :\|\|\|\| \|\|\|\|\|\|\|@LB@ GCUAA GCACUUC@LB@3'-AUU...UUUAG U AUUUU...AUG-5'@LB@ \| \|@LB@ 56 42@LB@@LB@@LB@Energy -7.58 kcal/mol@LB@Hybridization Energy -13.4464 kcal/mol@LB@Unfolding Energy - mRNA 2.60751 kcal/mol@LB@Unfolding Energy - sRNA 3.2693 kcal/mol@LB@@LB@Position - mRNA 202 -- 213@LB@Position - sRNA 43 -- 55@LB@Position Seed - mRNA 207 -- 213@LB@Position Seed - sRNA 43 -- 49@LB@
42	0.0004615	0.03766	b3341	rpsG	-11.13	0.020425	227 -- 249	53 -- 76	30S ribosomal subunit protein S7	_____________226______________________250\n_____________\|________________________\|\n5'-CGG...GGUCA__GU________U___________AGUUC...AUG-3'\n______________GC__AAA_AUUC_GCCGGAUCCGA\n______________\|\|__\|\|\|_\|\|\|\|_\|\|\|::\|\|\|\|\|\|\n______________CG__UUU_UAAG_CGGUUUAGGCU\n3'-AUU...CCCGC__AU___A____U___________AAUGC...AUG-5'\n_____________\|________________________\|\n_____________77_______________________52\n	243 -- 249	53 -- 59	-23.8099 kcal/mol	6.33566 kcal/mol	6.36489 kcal/mol	226 250@LB@ \| \|@LB@5'-CGG...GGUCA GU U AGUUC...AUG-3'@LB@ GC AAA AUUC GCCGGAUCCGA@LB@ \|\| \|\|\| \|\|\|\| \|\|\|::\|\|\|\|\|\|@LB@ CG UUU UAAG CGGUUUAGGCU@LB@3'-AUU...CCCGC AU A U AAUGC...AUG-5'@LB@ \| \|@LB@ 77 52@LB@@LB@@LB@Energy -11.13 kcal/mol@LB@Hybridization Energy -23.8099 kcal/mol@LB@Unfolding Energy - mRNA 6.33566 kcal/mol@LB@Unfolding Energy - sRNA 6.36489 kcal/mol@LB@@LB@Position - mRNA 227 -- 249@LB@Position - sRNA 53 -- 76@LB@Position Seed - mRNA 243 -- 249@LB@Position Seed - sRNA 53 -- 59@LB@
200	0.009019	0.1567	b3343	tusB	-6.18	0.382258	145 -- 152	1 -- 8	mnm(5)-s(2)U34-tRNA synthesis 2-thiolation protein	_____________144______153\n_____________\|________\|\n5'-GCU...GCAGA________GCCGC...GCG-3'\n______________UGCCUUAC\n______________\|:\|\|:\|\|\|\n______________AUGGGAUG\n3'-AUU...GAGAC________-5'\n_____________\|_______\|\n_____________9_______1\n	146 -- 152	1 -- 7	-9.97017 kcal/mol	1.6972 kcal/mol	2.11603 kcal/mol	144 153@LB@ \| \|@LB@5'-GCU...GCAGA GCCGC...GCG-3'@LB@ UGCCUUAC@LB@ \|:\|\|:\|\|\|@LB@ AUGGGAUG@LB@3'-AUU...GAGAC -5'@LB@ \| \|@LB@ 9 1@LB@@LB@@LB@Energy -6.18 kcal/mol@LB@Hybridization Energy -9.97017 kcal/mol@LB@Unfolding Energy - mRNA 1.6972 kcal/mol@LB@Unfolding Energy - sRNA 2.11603 kcal/mol@LB@@LB@Position - mRNA 145 -- 152@LB@Position - sRNA 1 -- 8@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 1 -- 7@LB@
130	0.004431	0.1199	b3355	prkB	-7.29	0.227256	214 -- 220	52 -- 58	putative phosphoribulokinase	_____________213_____221\n_____________\|_______\|\n5'-GGC...CAAAC_______CAUUG...AUC-3'\n______________AUCCGGU\n______________\|\|\|\|\|:\|\n______________UAGGCUA\n3'-AUU...CGGUU_______AUGCA...AUG-5'\n_____________\|_______\|\n_____________59______51\n	214 -- 220	52 -- 58	-8.26146 kcal/mol	0.695321 kcal/mol	0.296688 kcal/mol	213 221@LB@ \| \|@LB@5'-GGC...CAAAC CAUUG...AUC-3'@LB@ AUCCGGU@LB@ \|\|\|\|\|:\|@LB@ UAGGCUA@LB@3'-AUU...CGGUU AUGCA...AUG-5'@LB@ \| \|@LB@ 59 51@LB@@LB@@LB@Energy -7.29 kcal/mol@LB@Hybridization Energy -8.26146 kcal/mol@LB@Unfolding Energy - mRNA 0.695321 kcal/mol@LB@Unfolding Energy - sRNA 0.296688 kcal/mol@LB@@LB@Position - mRNA 214 -- 220@LB@Position - sRNA 52 -- 58@LB@Position Seed - mRNA 214 -- 220@LB@Position Seed - sRNA 52 -- 58@LB@
19	8.831e-05	0.01635	b3389	aroB	-10.54	0.031183	4 -- 17	42 -- 56	3-dehydroquinate synthase	_____________3_______________18\n_____________\|_______________\|\n________5'-ACA___CC__________UCUGG...CGG-3'\n______________CCG___GCGUGAAGU\n______________\|\|\|___:\|\|\|\|\|\|\|\|\n______________GGC___UGCACUUCA\n3'-AUU...GUUUA___UAA_________UUUUC...AUG-5'\n_____________\|_______________\|\n_____________57______________41\n	11 -- 17	42 -- 48	-15.9415 kcal/mol	2.13198 kcal/mol	3.27911 kcal/mol	3 18@LB@ \| \|@LB@ 5'-ACA CC UCUGG...CGG-3'@LB@ CCG GCGUGAAGU@LB@ \|\|\| :\|\|\|\|\|\|\|\|@LB@ GGC UGCACUUCA@LB@3'-AUU...GUUUA UAA UUUUC...AUG-5'@LB@ \| \|@LB@ 57 41@LB@@LB@@LB@Energy -10.54 kcal/mol@LB@Hybridization Energy -15.9415 kcal/mol@LB@Unfolding Energy - mRNA 2.13198 kcal/mol@LB@Unfolding Energy - sRNA 3.27911 kcal/mol@LB@@LB@Position - mRNA 4 -- 17@LB@Position - sRNA 42 -- 56@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 42 -- 48@LB@
188	0.008316	0.1556	b3406	greB	-8.11	0.146040	42 -- 55	52 -- 65	transcript cleavage factor	_____________41_____________56\n_____________\|______________\|\n5'-AUU...GUCAC______________AUGAA...UGA-3'\n______________CAGCUAAAUCUGGU\n______________\|\|\|\|:\|\|\|\|\|:\|:\|\n______________GUCGGUUUAGGCUA\n3'-AUU...UAUAA______________AUGCA...AUG-5'\n_____________\|______________\|\n_____________66_____________51\n	49 -- 55	52 -- 58	-18.5321 kcal/mol	4.27254 kcal/mol	6.17277 kcal/mol	41 56@LB@ \| \|@LB@5'-AUU...GUCAC AUGAA...UGA-3'@LB@ CAGCUAAAUCUGGU@LB@ \|\|\|\|:\|\|\|\|\|:\|:\|@LB@ GUCGGUUUAGGCUA@LB@3'-AUU...UAUAA AUGCA...AUG-5'@LB@ \| \|@LB@ 66 51@LB@@LB@@LB@Energy -8.11 kcal/mol@LB@Hybridization Energy -18.5321 kcal/mol@LB@Unfolding Energy - mRNA 4.27254 kcal/mol@LB@Unfolding Energy - sRNA 6.17277 kcal/mol@LB@@LB@Position - mRNA 42 -- 55@LB@Position - sRNA 52 -- 65@LB@Position Seed - mRNA 49 -- 55@LB@Position Seed - sRNA 52 -- 58@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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