Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
20	0.0008552	0.1448	b4137	cutA	-9.23	0.162618	220 -- 236	1 -- 16	divalent-cation tolerance protein copper sensitivity	_____________219_______________237\n_____________\|_________________\|\n5'-CAA...AAGUU_____AC_______G__GUGGU...CGG-3'\n______________CGAAU__CGCGUCU_UC\n______________\|:\|\|\|__\|\|\|\|\|\|\|_\|\|\n______________GUUUA__GCGCAGA_AG\n3'-UUU...CUAUU_____C________A__-5'\n_____________\|________________\|\n_____________17_______________1\n	227 -- 233	4 -- 10	-14.9299 kcal/mol	4.46216 kcal/mol	1.23857 kcal/mol	219 237@LB@ \| \|@LB@5'-CAA...AAGUU AC G GUGGU...CGG-3'@LB@ CGAAU CGCGUCU UC@LB@ \|:\|\|\| \|\|\|\|\|\|\| \|\|@LB@ GUUUA GCGCAGA AG@LB@3'-UUU...CUAUU C A -5'@LB@ \| \|@LB@ 17 1@LB@@LB@@LB@Energy -9.23 kcal/mol@LB@Hybridization Energy -14.9299 kcal/mol@LB@Unfolding Energy - mRNA 4.46216 kcal/mol@LB@Unfolding Energy - sRNA 1.23857 kcal/mol@LB@@LB@Position - mRNA 220 -- 236@LB@Position - sRNA 1 -- 16@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 4 -- 10@LB@
113	0.01159	0.3104	b1279	yciS	-9.20	0.164515	90 -- 101	6 -- 18	DUF1049 family inner membrane protein function unknown	_____________89____________102\n_____________\|_____________\|\n5'-ACC...UCAUC_____________GGAUG...UGU-3'\n______________AAC_AAUGCGUGU\n______________\|\|\|_\|\|\|\|\|\|:\|\|\n______________UUG_UUACGCGCA\n3'-UUU...UACUA___U_________GAAAG-5'\n_____________\|_____________\|\n_____________19____________5\n	95 -- 101	6 -- 12	-13.4585 kcal/mol	3.04139 kcal/mol	1.21878 kcal/mol	89 102@LB@ \| \|@LB@5'-ACC...UCAUC GGAUG...UGU-3'@LB@ AAC AAUGCGUGU@LB@ \|\|\| \|\|\|\|\|\|:\|\|@LB@ UUG UUACGCGCA@LB@3'-UUU...UACUA U GAAAG-5'@LB@ \| \|@LB@ 19 5@LB@@LB@@LB@Energy -9.20 kcal/mol@LB@Hybridization Energy -13.4585 kcal/mol@LB@Unfolding Energy - mRNA 3.04139 kcal/mol@LB@Unfolding Energy - sRNA 1.21878 kcal/mol@LB@@LB@Position - mRNA 90 -- 101@LB@Position - sRNA 6 -- 18@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 6 -- 12@LB@
177	0.01751	0.3104	b2900	yqfB	-9.18	0.165418	52 -- 70	1 -- 17	UPF0267 family protein	_____________51__________________71\n_____________\|___________________\|\n5'-UUC...ACGUC________U__GCC_____CCGCC...UCA-3'\n______________ACAAAUGC_CG___CUUUU\n______________\|\|\|\|\|\|\|\|_\|\|___\|\|\|\|:\n______________UGUUUACG_GC___GAAAG\n3'-UUU...ACUAU________C__A_______-5'\n_____________\|__________________\|\n_____________18_________________1\n	53 -- 59	10 -- 16	-13.5578 kcal/mol	3.13415 kcal/mol	1.23994 kcal/mol	51 71@LB@ \| \|@LB@5'-UUC...ACGUC U GCC CCGCC...UCA-3'@LB@ ACAAAUGC CG CUUUU@LB@ \|\|\|\|\|\|\|\| \|\| \|\|\|\|:@LB@ UGUUUACG GC GAAAG@LB@3'-UUU...ACUAU C A -5'@LB@ \| \|@LB@ 18 1@LB@@LB@@LB@Energy -9.18 kcal/mol@LB@Hybridization Energy -13.5578 kcal/mol@LB@Unfolding Energy - mRNA 3.13415 kcal/mol@LB@Unfolding Energy - sRNA 1.23994 kcal/mol@LB@@LB@Position - mRNA 52 -- 70@LB@Position - sRNA 1 -- 17@LB@Position Seed - mRNA 53 -- 59@LB@Position Seed - sRNA 10 -- 16@LB@
53	0.004896	0.3024	b3790	wecD	-9.15	0.167607	125 -- 139	7 -- 21	TDP-fucosamine acetyltransferase	_____________124_____________140\n_____________\|_______________\|\n5'-CUG...GGCUC_________G_____AAGCG...UGA-3'\n______________GAUGACGAG_GUGUG\n______________\|\|\|:\|\|:\|:_\|:\|:\|\n______________CUAUUGUUU_CGCGC\n3'-UUU...UACUA_________A_____AGAAAG-5'\n_____________\|_______________\|\n_____________22______________6\n	126 -- 132	14 -- 20	-15.9636 kcal/mol	5.57309 kcal/mol	1.24179 kcal/mol	124 140@LB@ \| \|@LB@5'-CUG...GGCUC G AAGCG...UGA-3'@LB@ GAUGACGAG GUGUG@LB@ \|\|\|:\|\|:\|: \|:\|:\|@LB@ CUAUUGUUU CGCGC@LB@3'-UUU...UACUA A AGAAAG-5'@LB@ \| \|@LB@ 22 6@LB@@LB@@LB@Energy -9.15 kcal/mol@LB@Hybridization Energy -15.9636 kcal/mol@LB@Unfolding Energy - mRNA 5.57309 kcal/mol@LB@Unfolding Energy - sRNA 1.24179 kcal/mol@LB@@LB@Position - mRNA 125 -- 139@LB@Position - sRNA 7 -- 21@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 14 -- 20@LB@
61	0.005608	0.3084	b2174	lpxT	-9.09	0.171357	170 -- 179	1 -- 10	lipid A 1-diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1-phosphate phosphotransferase	_____________169________180\n_____________\|__________\|\n5'-GAA...UUGCC__________AGUAA...CGA-3'\n______________UGCGUUUUUC\n______________:\|\|\|\|:\|\|\|\|\n______________GCGCAGAAAG\n3'-UUU...UUUAC__________-5'\n_____________\|_________\|\n_____________11________1\n	173 -- 179	1 -- 7	-13.1644 kcal/mol	2.90849 kcal/mol	1.16635 kcal/mol	169 180@LB@ \| \|@LB@5'-GAA...UUGCC AGUAA...CGA-3'@LB@ UGCGUUUUUC@LB@ :\|\|\|\|:\|\|\|\|@LB@ GCGCAGAAAG@LB@3'-UUU...UUUAC -5'@LB@ \| \|@LB@ 11 1@LB@@LB@@LB@Energy -9.09 kcal/mol@LB@Hybridization Energy -13.1644 kcal/mol@LB@Unfolding Energy - mRNA 2.90849 kcal/mol@LB@Unfolding Energy - sRNA 1.16635 kcal/mol@LB@@LB@Position - mRNA 170 -- 179@LB@Position - sRNA 1 -- 10@LB@Position Seed - mRNA 173 -- 179@LB@Position Seed - sRNA 1 -- 7@LB@
42	0.002847	0.2296	b1638	pdxH	-9.07	0.172497	29 -- 35	5 -- 11	pyridoxine 5'-phosphate oxidase	_____________28______36\n_____________\|_______\|\n5'-UCU...CUGGC_______ACACU...CCC-3'\n______________GCGCGUU\n______________\|\|\|\|\|\|:\n______________CGCGCAG\n3'-UUU...GUUUA_______AAAG-5'\n_____________\|_______\|\n_____________12______4\n	29 -- 35	5 -- 11	-12.2635 kcal/mol	2.08033 kcal/mol	1.11145 kcal/mol	28 36@LB@ \| \|@LB@5'-UCU...CUGGC ACACU...CCC-3'@LB@ GCGCGUU@LB@ \|\|\|\|\|\|:@LB@ CGCGCAG@LB@3'-UUU...GUUUA AAAG-5'@LB@ \| \|@LB@ 12 4@LB@@LB@@LB@Energy -9.07 kcal/mol@LB@Hybridization Energy -12.2635 kcal/mol@LB@Unfolding Energy - mRNA 2.08033 kcal/mol@LB@Unfolding Energy - sRNA 1.11145 kcal/mol@LB@@LB@Position - mRNA 29 -- 35@LB@Position - sRNA 5 -- 11@LB@Position Seed - mRNA 29 -- 35@LB@Position Seed - sRNA 5 -- 11@LB@
174	0.01734	0.3104	b3470	tusA	-9.03	0.174990	268 -- 281	8 -- 22	mnm(5)-s(2)U34-tRNA 2-thiolation sulfurtransferase	_____________267_____________282\n_____________\|_______________\|\n5'-CAU...ACCGG_______________AAAAC...UGC-3'\n______________UGAUGAU_GGUGCGC\n______________\|\|\|\|:\|:_::\|\|\|\|\|\n______________ACUAUUG_UUACGCG\n3'-UUU...CUACU_______U_______CAGAAAG-5'\n_____________\|_______________\|\n_____________23______________7\n	275 -- 281	8 -- 14	-15.1295 kcal/mol	4.85785 kcal/mol	1.23838 kcal/mol	267 282@LB@ \| \|@LB@5'-CAU...ACCGG AAAAC...UGC-3'@LB@ UGAUGAU GGUGCGC@LB@ \|\|\|\|:\|: ::\|\|\|\|\|@LB@ ACUAUUG UUACGCG@LB@3'-UUU...CUACU U CAGAAAG-5'@LB@ \| \|@LB@ 23 7@LB@@LB@@LB@Energy -9.03 kcal/mol@LB@Hybridization Energy -15.1295 kcal/mol@LB@Unfolding Energy - mRNA 4.85785 kcal/mol@LB@Unfolding Energy - sRNA 1.23838 kcal/mol@LB@@LB@Position - mRNA 268 -- 281@LB@Position - sRNA 8 -- 22@LB@Position Seed - mRNA 275 -- 281@LB@Position Seed - sRNA 8 -- 14@LB@
154	0.01456	0.3104	b2048	cpsG	-9.02	0.175967	42 -- 71	4 -- 44	phosphomannomutase	_____________41________________________________________72\n_____________\|_________________________________________\|\n5'-GGG...GGCUC___________GA___________UG_______________CGCGG...GCG-3'\n______________UUAUCUC_GAA_____GGAUGAUG_________GUGCGUUU\n______________\|:\|\|\|\|\|_\|\|\|_____\|\|\|\|\|\|\|\|_________\|:\|\|\|\|:\|\n______________AGUAGAG_CUU_____CCUACUAC_________CGCGCAGA\n3'-UUU...UUUAA_______A___AAGUC________UAUUGUUUA________AAG-5'\n_____________\|_________________________________________\|\n_____________45________________________________________3\n	65 -- 71	4 -- 10	-28.9111 kcal/mol	10.4373 kcal/mol	9.45546 kcal/mol	41 72@LB@ \| \|@LB@5'-GGG...GGCUC GA UG CGCGG...GCG-3'@LB@ UUAUCUC GAA GGAUGAUG GUGCGUUU@LB@ \|:\|\|\|\|\| \|\|\| \|\|\|\|\|\|\|\| \|:\|\|\|\|:\|@LB@ AGUAGAG CUU CCUACUAC CGCGCAGA@LB@3'-UUU...UUUAA A AAGUC UAUUGUUUA AAG-5'@LB@ \| \|@LB@ 45 3@LB@@LB@@LB@Energy -9.02 kcal/mol@LB@Hybridization Energy -28.9111 kcal/mol@LB@Unfolding Energy - mRNA 10.4373 kcal/mol@LB@Unfolding Energy - sRNA 9.45546 kcal/mol@LB@@LB@Position - mRNA 42 -- 71@LB@Position - sRNA 4 -- 44@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 4 -- 10@LB@
54	0.004897	0.3024	b1949	fliQ	-8.93	0.181731	208 -- 239	7 -- 38	flagellar biosynthesis protein	_____________207_______________________________240\n_____________\|_________________________________\|\n5'-UUC...GACAC__________UC__________G__C__AA___AUGAA...UUA-3'\n______________CUGAA_UCGG__AUGAUGAUGG_GA_UG__GCG\n______________\|\|\|\|\|_\|\|:\|__\|\|\|\|\|\|\|\|::_:\|_\|\|__\|\|\|\n______________GACUU_AGUC__UACUACUAUU_UU_AC__CGC\n3'-UUU...GUAGA_____A____CC__________G__U__G____AGAAAG-5'\n_____________\|_________________________________\|\n_____________39________________________________6\n	222 -- 228	17 -- 23	-27.1148 kcal/mol	8.82457 kcal/mol	9.35859 kcal/mol	207 240@LB@ \| \|@LB@5'-UUC...GACAC UC G C AA AUGAA...UUA-3'@LB@ CUGAA UCGG AUGAUGAUGG GA UG GCG@LB@ \|\|\|\|\| \|\|:\| \|\|\|\|\|\|\|\|:: :\| \|\| \|\|\|@LB@ GACUU AGUC UACUACUAUU UU AC CGC@LB@3'-UUU...GUAGA A CC G U G AGAAAG-5'@LB@ \| \|@LB@ 39 6@LB@@LB@@LB@Energy -8.93 kcal/mol@LB@Hybridization Energy -27.1148 kcal/mol@LB@Unfolding Energy - mRNA 8.82457 kcal/mol@LB@Unfolding Energy - sRNA 9.35859 kcal/mol@LB@@LB@Position - mRNA 208 -- 239@LB@Position - sRNA 7 -- 38@LB@Position Seed - mRNA 222 -- 228@LB@Position Seed - sRNA 17 -- 23@LB@
88	0.008928	0.3104	b0405	queA	-8.89	0.184414	201 -- 209	5 -- 13	S-adenosylmethionine:tRNA ribosyltransferase-isomerase	_____________200_______210\n_____________\|_________\|\n5'-GGG...GUGUC_________ACCGA...GGC-3'\n______________AUGCGCGUU\n______________\|\|\|\|\|\|\|\|:\n______________UACGCGCAG\n3'-UUU...UUGUU_________AAAG-5'\n_____________\|_________\|\n_____________14________4\n	203 -- 209	5 -- 11	-14.1161 kcal/mol	4.07268 kcal/mol	1.15134 kcal/mol	200 210@LB@ \| \|@LB@5'-GGG...GUGUC ACCGA...GGC-3'@LB@ AUGCGCGUU@LB@ \|\|\|\|\|\|\|\|:@LB@ UACGCGCAG@LB@3'-UUU...UUGUU AAAG-5'@LB@ \| \|@LB@ 14 4@LB@@LB@@LB@Energy -8.89 kcal/mol@LB@Hybridization Energy -14.1161 kcal/mol@LB@Unfolding Energy - mRNA 4.07268 kcal/mol@LB@Unfolding Energy - sRNA 1.15134 kcal/mol@LB@@LB@Position - mRNA 201 -- 209@LB@Position - sRNA 5 -- 13@LB@Position Seed - mRNA 203 -- 209@LB@Position Seed - sRNA 5 -- 11@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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