Input and runtime details for job 1786318 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1786318 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:20:27 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:20:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:01:46 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:01:53 CET 2018
	Job Completed		@ Fri Feb 16 22:02:22 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1786318&toolName=CopraRNA ( 30 days expiry )

Description of the job

ArcZ

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
64	0.003238	0.1727	b2578	eamB	-8.70	0.240388	240 -- 262	70 -- 85	cysteine and O-acetylserine exporter	_____________239_____________________263\n_____________\|_______________________\|\n5'-GUC...ACACC___AUUAC____AU_________ACCGA...CGC-3'\n______________CUG_____CGCU__GACGCCAGG\n______________\|\|\|_____\|\|\|:__:\|\|:\|\|\|\|\|\n______________GAC_____GCGG__UUGUGGUCC\n3'-UUU...CUUAU_______________________CUUUA...GUG-5'\n_____________\|_______________________\|\n_____________86______________________69\n	256 -- 262	70 -- 76	-20.6717 kcal/mol	4.69447 kcal/mol	7.27689 kcal/mol	239 263@LB@ \| \|@LB@5'-GUC...ACACC AUUAC AU ACCGA...CGC-3'@LB@ CUG CGCU GACGCCAGG@LB@ \|\|\| \|\|\|: :\|\|:\|\|\|\|\|@LB@ GAC GCGG UUGUGGUCC@LB@3'-UUU...CUUAU CUUUA...GUG-5'@LB@ \| \|@LB@ 86 69@LB@@LB@@LB@Energy -8.70 kcal/mol@LB@Hybridization Energy -20.6717 kcal/mol@LB@Unfolding Energy - mRNA 4.69447 kcal/mol@LB@Unfolding Energy - sRNA 7.27689 kcal/mol@LB@@LB@Position - mRNA 240 -- 262@LB@Position - sRNA 70 -- 85@LB@Position Seed - mRNA 256 -- 262@LB@Position Seed - sRNA 70 -- 76@LB@
65	0.003263	0.1727	b2584	pka	-7.67	0.359085	49 -- 62	69 -- 82	protein lysine acetyltransferase	_____________48_____________63\n_____________\|______________\|\n5'-AGG...AUGGC__G___________CGGCG...GUA-3'\n______________CG_CGAUACCGGGG\n______________\|\|_\|:\|:\|\|\|:\|\|\|\n______________GC_GUUGUGGUCCC\n3'-UUU...AUGAC__G___________UUUAG...GUG-5'\n_____________\|______________\|\n_____________83_____________68\n	56 -- 62	69 -- 75	-21.7159 kcal/mol	7.18281 kcal/mol	6.85849 kcal/mol	48 63@LB@ \| \|@LB@5'-AGG...AUGGC G CGGCG...GUA-3'@LB@ CG CGAUACCGGGG@LB@ \|\| \|:\|:\|\|\|:\|\|\|@LB@ GC GUUGUGGUCCC@LB@3'-UUU...AUGAC G UUUAG...GUG-5'@LB@ \| \|@LB@ 83 68@LB@@LB@@LB@Energy -7.67 kcal/mol@LB@Hybridization Energy -21.7159 kcal/mol@LB@Unfolding Energy - mRNA 7.18281 kcal/mol@LB@Unfolding Energy - sRNA 6.85849 kcal/mol@LB@@LB@Position - mRNA 49 -- 62@LB@Position - sRNA 69 -- 82@LB@Position Seed - mRNA 56 -- 62@LB@Position Seed - sRNA 69 -- 75@LB@
192	0.01187	0.2159	b2600	tyrA	-9.34	0.184498	136 -- 155	70 -- 86	fused chorismate mutase T/prephenate dehydrogenase	_____________135__________________156\n_____________\|____________________\|\n5'-UCG...GCCUU_______G__AUU_______AUCUG...UGG-3'\n______________GCUGCGU_AA___CAUCAGG\n______________:\|\|\|\|\|:_\|\|___\|\|:\|\|\|\|\n______________UGACGCG_UU___GUGGUCC\n3'-UUU...GCUUA_______G____________CUUUA...GUG-5'\n_____________\|____________________\|\n_____________87___________________69\n	149 -- 155	70 -- 76	-22.0981 kcal/mol	5.48162 kcal/mol	7.27772 kcal/mol	135 156@LB@ \| \|@LB@5'-UCG...GCCUU G AUU AUCUG...UGG-3'@LB@ GCUGCGU AA CAUCAGG@LB@ :\|\|\|\|\|: \|\| \|\|:\|\|\|\|@LB@ UGACGCG UU GUGGUCC@LB@3'-UUU...GCUUA G CUUUA...GUG-5'@LB@ \| \|@LB@ 87 69@LB@@LB@@LB@Energy -9.34 kcal/mol@LB@Hybridization Energy -22.0981 kcal/mol@LB@Unfolding Energy - mRNA 5.48162 kcal/mol@LB@Unfolding Energy - sRNA 7.27772 kcal/mol@LB@@LB@Position - mRNA 136 -- 155@LB@Position - sRNA 70 -- 86@LB@Position Seed - mRNA 149 -- 155@LB@Position Seed - sRNA 70 -- 76@LB@
154	0.009082	0.2059	b2676	nrdF	-13.94	0.023769	159 -- 176	53 -- 69	ribonucleoside-diphosphate reductase 2 beta subunit ferritin-like protein	_____________158________________177\n_____________\|__________________\|\n5'-AAG...GCACU______GAA_________UCCUG...CAG-3'\n______________GAAAUU___GGCUGCGUC\n______________\|\|\|\|\|:___\|\|\|\|\|\|\|:\|\n______________CUUUAG___CCGACGCGG\n3'-UUU...GGUCC______AA__________CAUUU...GUG-5'\n_____________\|__________________\|\n_____________70_________________52\n	170 -- 176	53 -- 59	-23.1658 kcal/mol	2.38862 kcal/mol	6.83371 kcal/mol	158 177@LB@ \| \|@LB@5'-AAG...GCACU GAA UCCUG...CAG-3'@LB@ GAAAUU GGCUGCGUC@LB@ \|\|\|\|\|: \|\|\|\|\|\|\|:\|@LB@ CUUUAG CCGACGCGG@LB@3'-UUU...GGUCC AA CAUUU...GUG-5'@LB@ \| \|@LB@ 70 52@LB@@LB@@LB@Energy -13.94 kcal/mol@LB@Hybridization Energy -23.1658 kcal/mol@LB@Unfolding Energy - mRNA 2.38862 kcal/mol@LB@Unfolding Energy - sRNA 6.83371 kcal/mol@LB@@LB@Position - mRNA 159 -- 176@LB@Position - sRNA 53 -- 69@LB@Position Seed - mRNA 170 -- 176@LB@Position Seed - sRNA 53 -- 59@LB@
156	0.009123	0.2059	b2683	ygaH	-13.79	0.025463	164 -- 213	27 -- 86	putative L-valine exporter norvaline resistance protein	_____________163______________________________________________________________214\n_____________\|________________________________________________________________\|\n5'-AUU...GCCUC_________________CAU________AAGGA_____C_________________C_______UUCUG...CAA-3'\n______________ACUGCGUUAAU_CCAGG____UC_UGGC_____GCGCC____________GAUGAG_UAUGAGG\n______________\|\|\|\|\|\|::\|\|:_\|\|\|\|\|____\|\|_\|\|\|\|_____\|\|\|\|\|____________\|\|\|\|\|\|_\|\|::\|\|\|\n______________UGACGCGGUUG_GGUCC____AG_ACCG_____CGCGG____________CUACUC_AUGUUCC\n3'-UUU...GCUUA___________U_____CUUU__A____A_________CAUUUAAUAAUA______A_______CGUGU...GUG-5'\n_____________\|________________________________________________________________\|\n_____________87_______________________________________________________________26\n	207 -- 213	27 -- 33	-50.2075 kcal/mol	19.3484 kcal/mol	17.0667 kcal/mol	163 214@LB@ \| \|@LB@5'-AUU...GCCUC CAU AAGGA C C UUCUG...CAA-3'@LB@ ACUGCGUUAAU CCAGG UC UGGC GCGCC GAUGAG UAUGAGG@LB@ \|\|\|\|\|\|::\|\|: \|\|\|\|\| \|\| \|\|\|\| \|\|\|\|\| \|\|\|\|\|\| \|\|::\|\|\|@LB@ UGACGCGGUUG GGUCC AG ACCG CGCGG CUACUC AUGUUCC@LB@3'-UUU...GCUUA U CUUU A A CAUUUAAUAAUA A CGUGU...GUG-5'@LB@ \| \|@LB@ 87 26@LB@@LB@@LB@Energy -13.79 kcal/mol@LB@Hybridization Energy -50.2075 kcal/mol@LB@Unfolding Energy - mRNA 19.3484 kcal/mol@LB@Unfolding Energy - sRNA 17.0667 kcal/mol@LB@@LB@Position - mRNA 164 -- 213@LB@Position - sRNA 27 -- 86@LB@Position Seed - mRNA 207 -- 213@LB@Position Seed - sRNA 27 -- 33@LB@
94	0.005015	0.1855	b2703	srlE	-7.35	0.404948	254 -- 271	70 -- 86	glucitol/sorbitol-specific enzyme IIB component of PTS	_____________253_________________272\n_____________\|___________________\|\n5'-GAA...CUUGA____AAA____________AAAAA...GUA-3'\n______________GCUG___GCC_ACGCCGGG\n______________:\|\|\|___\|\|\|_\|\|:\|\|:\|\|\n______________UGAC___CGG_UGUGGUCC\n3'-UUU...GCUUA____G_____U________CUUUA...GUG-5'\n_____________\|___________________\|\n_____________87__________________69\n	265 -- 271	70 -- 76	-23.089 kcal/mol	8.46506 kcal/mol	7.27772 kcal/mol	253 272@LB@ \| \|@LB@5'-GAA...CUUGA AAA AAAAA...GUA-3'@LB@ GCUG GCC ACGCCGGG@LB@ :\|\|\| \|\|\| \|\|:\|\|:\|\|@LB@ UGAC CGG UGUGGUCC@LB@3'-UUU...GCUUA G U CUUUA...GUG-5'@LB@ \| \|@LB@ 87 69@LB@@LB@@LB@Energy -7.35 kcal/mol@LB@Hybridization Energy -23.089 kcal/mol@LB@Unfolding Energy - mRNA 8.46506 kcal/mol@LB@Unfolding Energy - sRNA 7.27772 kcal/mol@LB@@LB@Position - mRNA 254 -- 271@LB@Position - sRNA 70 -- 86@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 70 -- 76@LB@
52	0.002619	0.1727	b2720	hycF	-12.75	0.040951	8 -- 55	44 -- 94	formate hydrogenlyase complex iron-sulfur protein	_____________7______________________________________________________56\n_____________\|______________________________________________________\|\n____5'-CGACCCU____UAC_________GG_________________CCGA_______CU______CGGUA...CAG-3'\n______________GCGC___AUGCUGCGC__CAACACC______GUUU____UGCGCCG__GAUUAU\n______________\|\|\|\|___\|\|:\|\|\|\|\|\|__\|\|\|\|\|\|\|______:\|:\|____\|\|\|\|\|\|\|__:\|\|\|\|\|\n______________CGCG___UAUGACGCG__GUUGUGG______UAGA____ACGCGGC__UUAAUA\n3'-UUU...CCCCA____CU___________________UCCCUU____ACCG_______AU______AUACU...GUG-5'\n_____________\|______________________________________________________\|\n_____________95_____________________________________________________43\n	41 -- 47	52 -- 58	-46.3174 kcal/mol	16.2176 kcal/mol	17.3533 kcal/mol	7 56@LB@ \| \|@LB@ 5'-CGACCCU UAC GG CCGA CU CGGUA...CAG-3'@LB@ GCGC AUGCUGCGC CAACACC GUUU UGCGCCG GAUUAU@LB@ \|\|\|\| \|\|:\|\|\|\|\|\| \|\|\|\|\|\|\| :\|:\| \|\|\|\|\|\|\| :\|\|\|\|\|@LB@ CGCG UAUGACGCG GUUGUGG UAGA ACGCGGC UUAAUA@LB@3'-UUU...CCCCA CU UCCCUU ACCG AU AUACU...GUG-5'@LB@ \| \|@LB@ 95 43@LB@@LB@@LB@Energy -12.75 kcal/mol@LB@Hybridization Energy -46.3174 kcal/mol@LB@Unfolding Energy - mRNA 16.2176 kcal/mol@LB@Unfolding Energy - sRNA 17.3533 kcal/mol@LB@@LB@Position - mRNA 8 -- 55@LB@Position - sRNA 44 -- 94@LB@Position Seed - mRNA 41 -- 47@LB@Position Seed - sRNA 52 -- 58@LB@
103	0.005609	0.1855	b2912	fau	-11.92	0.059425	103 -- 124	64 -- 85	5-formyltetrahydrofolate cyclo-ligase family protein	_____________102____________________125\n_____________\|______________________\|\n5'-GAA...ACAGC_____G_______UC_______CCCUU...UGG-3'\n______________CUGCG_CGGCAUC__GGAGAUU\n______________\|\|\|\|\|_\|::\|\|:\|__\|\|\|:\|\|:\n______________GACGC_GUUGUGG__CCUUUAG\n3'-UUU...CUUAU_____G_______UC_______AACCG...GUG-5'\n_____________\|______________________\|\n_____________86_____________________63\n	118 -- 124	64 -- 70	-27.7471 kcal/mol	8.44142 kcal/mol	7.38387 kcal/mol	102 125@LB@ \| \|@LB@5'-GAA...ACAGC G UC CCCUU...UGG-3'@LB@ CUGCG CGGCAUC GGAGAUU@LB@ \|\|\|\|\| \|::\|\|:\| \|\|\|:\|\|:@LB@ GACGC GUUGUGG CCUUUAG@LB@3'-UUU...CUUAU G UC AACCG...GUG-5'@LB@ \| \|@LB@ 86 63@LB@@LB@@LB@Energy -11.92 kcal/mol@LB@Hybridization Energy -27.7471 kcal/mol@LB@Unfolding Energy - mRNA 8.44142 kcal/mol@LB@Unfolding Energy - sRNA 7.38387 kcal/mol@LB@@LB@Position - mRNA 103 -- 124@LB@Position - sRNA 64 -- 85@LB@Position Seed - mRNA 118 -- 124@LB@Position Seed - sRNA 64 -- 70@LB@
126	0.006849	0.1914	b2930	yggF	-13.28	0.032133	176 -- 191	68 -- 83	fructose 1 6 bisphosphatase isozyme	_____________175______________192\n_____________\|________________\|\n5'-AUC...AUUUG________________GGCCG...GCC-3'\n______________GCGUUGAUAUCGGGGA\n______________\|\|\|:::\|:\|:\|:\|\|\|\|\n______________CGCGGUUGUGGUCCCU\n3'-UUU...UAUGA________________UUAGA...GUG-5'\n_____________\|________________\|\n_____________84_______________67\n	185 -- 191	68 -- 74	-24.7766 kcal/mol	4.1491 kcal/mol	7.34657 kcal/mol	175 192@LB@ \| \|@LB@5'-AUC...AUUUG GGCCG...GCC-3'@LB@ GCGUUGAUAUCGGGGA@LB@ \|\|\|:::\|:\|:\|:\|\|\|\|@LB@ CGCGGUUGUGGUCCCU@LB@3'-UUU...UAUGA UUAGA...GUG-5'@LB@ \| \|@LB@ 84 67@LB@@LB@@LB@Energy -13.28 kcal/mol@LB@Hybridization Energy -24.7766 kcal/mol@LB@Unfolding Energy - mRNA 4.1491 kcal/mol@LB@Unfolding Energy - sRNA 7.34657 kcal/mol@LB@@LB@Position - mRNA 176 -- 191@LB@Position - sRNA 68 -- 83@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 68 -- 74@LB@
115	0.006085	0.1855	b3030	parE	-11.34	0.077071	10 -- 31	65 -- 86	DNA topoisomerase IV subunit B	_____________9______________________32\n_____________\|______________________\|\n5'-UUU...AUCUG_____UG_____GAC_______GAAGU...ACC-3'\n______________GCUGC__CAACA___GGGAGAU\n______________:\|\|\|\|__\|\|\|\|\|___\|\|\|\|:\|\|\n______________UGACG__GUUGU___CCCUUUA\n3'-UUU...GCUUA_____CG_____GGU_______GAACC...GUG-5'\n_____________\|______________________\|\n_____________87_____________________64\n	25 -- 31	65 -- 71	-24.4704 kcal/mol	5.74549 kcal/mol	7.38332 kcal/mol	9 32@LB@ \| \|@LB@5'-UUU...AUCUG UG GAC GAAGU...ACC-3'@LB@ GCUGC CAACA GGGAGAU@LB@ :\|\|\|\| \|\|\|\|\| \|\|\|\|:\|\|@LB@ UGACG GUUGU CCCUUUA@LB@3'-UUU...GCUUA CG GGU GAACC...GUG-5'@LB@ \| \|@LB@ 87 64@LB@@LB@@LB@Energy -11.34 kcal/mol@LB@Hybridization Energy -24.4704 kcal/mol@LB@Unfolding Energy - mRNA 5.74549 kcal/mol@LB@Unfolding Energy - sRNA 7.38332 kcal/mol@LB@@LB@Position - mRNA 10 -- 31@LB@Position - sRNA 65 -- 86@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 65 -- 71@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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