| 121 |
0.005491 |
0.1741 |
bsu40190 |
fbp |
-16.50 |
0.016068 |
67 -- 83 |
53 -- 69 |
fructose-1 6-bisphosphatase |
|
_____________66________________84\n_____________|_________________|\n5'-AAA...AAUGA___________U_____UAAAC...GCA-3'\n______________GGCAAACAAUC_UACCC\n______________|||||||||||_|||||\n______________CCGUUUGUUAG_AUGGG\n3'-AUU...UAGAA___________C_____UAGAG...CAA-5'\n_____________|_________________|\n_____________70________________52\n |
71 -- 77 |
59 -- 65 |
-25.6398 kcal/mol |
2.55798 kcal/mol |
7.79863 kcal/mol |
66 84@LB@ | |@LB@5'-AAA...AAUGA U UAAAC...GCA-3'@LB@ GGCAAACAAUC UACCC@LB@ ||||||||||| |||||@LB@ CCGUUUGUUAG AUGGG@LB@3'-AUU...UAGAA C UAGAG...CAA-5'@LB@ | |@LB@ 70 52@LB@@LB@@LB@Energy -16.50 kcal/mol@LB@Hybridization Energy -25.6398 kcal/mol@LB@Unfolding Energy - mRNA 2.55798 kcal/mol@LB@Unfolding Energy - sRNA 7.79863 kcal/mol@LB@@LB@Position - mRNA 67 -- 83@LB@Position - sRNA 53 -- 69@LB@Position Seed - mRNA 71 -- 77@LB@Position Seed - sRNA 59 -- 65@LB@ |
| 122 |
0.005613 |
0.1741 |
bsu19770 |
cgeC |
-14.56 |
0.041162 |
73 -- 119 |
6 -- 59 |
spore maturation protein CgeC |
|
_____________72_______________________________________________________120\n_____________|________________________________________________________|\n5'-CAC...AACGC_______G____AUUU___A_______AAG__________________________AAUGG...AAG-3'\n______________CGUACCU_AUCU____UUC_UCUAUUU___________GAAAAUGCU_GAUUUUUU\n______________||||||:_||||____|||_:|:||||___________|||||:|||_:||||:||\n______________GCAUGGG_UAGA____AAG_GGGUAAA___________CUUUUGCGA_UUAAAGAA\n3'-AUU...UGUUA____________GAAU___________GAAACUUUAAA_________A________AACAA-5'\n_____________|________________________________________________________|\n_____________60_______________________________________________________5\n |
105 -- 111 |
15 -- 21 |
-32.5895 kcal/mol |
8.55003 kcal/mol |
11.8347 kcal/mol |
72 120@LB@ | |@LB@5'-CAC...AACGC G AUUU A AAG AAUGG...AAG-3'@LB@ CGUACCU AUCU UUC UCUAUUU GAAAAUGCU GAUUUUUU@LB@ ||||||: |||| ||| :|:|||| |||||:||| :||||:||@LB@ GCAUGGG UAGA AAG GGGUAAA CUUUUGCGA UUAAAGAA@LB@3'-AUU...UGUUA GAAU GAAACUUUAAA A AACAA-5'@LB@ | |@LB@ 60 5@LB@@LB@@LB@Energy -14.56 kcal/mol@LB@Hybridization Energy -32.5895 kcal/mol@LB@Unfolding Energy - mRNA 8.55003 kcal/mol@LB@Unfolding Energy - sRNA 11.8347 kcal/mol@LB@@LB@Position - mRNA 73 -- 119@LB@Position - sRNA 6 -- 59@LB@Position Seed - mRNA 105 -- 111@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 123 |
0.005649 |
0.1741 |
bsu37530 |
ywhC |
-10.56 |
0.250674 |
18 -- 29 |
105 -- 116 |
zinc metalloprotease YwhC |
|
_____________17___________30\n_____________|____________|\n5'-GUU...GCUUC____________GACAA...UCG-3'\n______________UGUUACUUUCUC\n______________|||:||||||||\n______________ACAGUGAAAGAG\n3'-AUU...UUACA____________UAGAA...CAA-5'\n_____________|____________|\n_____________117__________104\n |
23 -- 29 |
105 -- 111 |
-16.5612 kcal/mol |
1.98074 kcal/mol |
5.11978 kcal/mol |
17 30@LB@ | |@LB@5'-GUU...GCUUC GACAA...UCG-3'@LB@ UGUUACUUUCUC@LB@ |||:||||||||@LB@ ACAGUGAAAGAG@LB@3'-AUU...UUACA UAGAA...CAA-5'@LB@ | |@LB@ 117 104@LB@@LB@@LB@Energy -10.56 kcal/mol@LB@Hybridization Energy -16.5612 kcal/mol@LB@Unfolding Energy - mRNA 1.98074 kcal/mol@LB@Unfolding Energy - sRNA 5.11978 kcal/mol@LB@@LB@Position - mRNA 18 -- 29@LB@Position - sRNA 105 -- 116@LB@Position Seed - mRNA 23 -- 29@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 124 |
0.005654 |
0.1741 |
bsu24680 |
comGF |
-17.71 |
0.008856 |
105 -- 249 |
40 -- 187 |
ComG operon protein 6 |
|
_____________104_____________________________________250\n_____________|_______________________________________|\n5'-UGU...UUUUG______A_______A_________GUU__A_A_______AAUGG...UAG-3'\n______________UC_UCG_CAGCAGG_ACA_UGACG___UC_C_CAGCAGG\n______________||_||:_||:||||_|||_||||:___||_|_|||||:|\n______________AG_AGU_GUUGUCC_UGU_ACUGU___AG_G_GUCGUUC\n3'-AUU...AAAUG__A___A___________A_____AAC____C_______AAGGU...CAA-5'\n_____________|_______________________________________|\n_____________188_____________________________________39\n |
276 -- 282 |
130 -- 136 |
-32.8645 kcal/mol |
8.22307 kcal/mol |
10.1234 kcal/mol |
104 250@LB@ | |@LB@5'-UGU...UUUUG A A GUU A A AAUGG...UAG-3'@LB@ UC UCG CAGCAGG ACA UGACG UC C CAGCAGG@LB@ || ||: ||:|||| ||| ||||: || | |||||:|@LB@ AG AGU GUUGUCC UGU ACUGU AG G GUCGUUC@LB@3'-AUU...AAAUG A A A AAC C AAGGU...CAA-5'@LB@ | |@LB@ 188 39@LB@@LB@@LB@Energy -17.71 kcal/mol@LB@Hybridization Energy -32.8645 kcal/mol@LB@Unfolding Energy - mRNA 8.22307 kcal/mol@LB@Unfolding Energy - sRNA 10.1234 kcal/mol@LB@@LB@Position - mRNA 105 -- 249@LB@Position - sRNA 40 -- 187@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 130 -- 136@LB@ |
| 125 |
0.005817 |
0.1741 |
bsu26980 |
yraE |
-11.00 |
0.208718 |
35 -- 90 |
112 -- 185 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________34________________________________________________________91\n_____________|_________________________________________________________|\n5'-UAC...UUUGA___AG______________AA_______A____GGA_____________________AGCCA...CUG-3'\n______________ACU___UAUCGACAGG_CA_____AUUG_UCCG_______________GAUGUUGUC\n______________|||___:|||:|||||_||_____||||_||||_______________:||||||||\n______________UGA___GUAGUUGUCC_GU_____UAAC_AGGC_______________UUACAACAG\n3'-AUU...AAAAA___GAA__________U__AACUG_________GUCGUUCAAGGUAUA_________UGAAA...CAA-5'\n_____________|_________________________________________________________|\n_____________186_______________________________________________________111\n |
83 -- 89 |
113 -- 119 |
-30.5681 kcal/mol |
8.54797 kcal/mol |
10.8348 kcal/mol |
34 91@LB@ | |@LB@5'-UAC...UUUGA AG AA A GGA AGCCA...CUG-3'@LB@ ACU UAUCGACAGG CA AUUG UCCG GAUGUUGUC@LB@ ||| :|||:||||| || |||| |||| :||||||||@LB@ UGA GUAGUUGUCC GU UAAC AGGC UUACAACAG@LB@3'-AUU...AAAAA GAA U AACUG GUCGUUCAAGGUAUA UGAAA...CAA-5'@LB@ | |@LB@ 186 111@LB@@LB@@LB@Energy -11.00 kcal/mol@LB@Hybridization Energy -30.5681 kcal/mol@LB@Unfolding Energy - mRNA 8.54797 kcal/mol@LB@Unfolding Energy - sRNA 10.8348 kcal/mol@LB@@LB@Position - mRNA 35 -- 90@LB@Position - sRNA 112 -- 185@LB@Position Seed - mRNA 83 -- 89@LB@Position Seed - sRNA 113 -- 119@LB@ |
| 126 |
0.005872 |
0.1741 |
bsu36510 |
amtB |
-10.53 |
0.253946 |
51 -- 89 |
33 -- 57 |
ammonium transporter NrgA |
|
_____________50_____________________________________________________________________________________90\n_____________|______________________________________________________________________________________|\n______5'-CUCAU______A_____UUUUAUAGAUACAA______AUAUC____AAAA____AU____C_______UUCCAUCCCUCGAUAA_______CAAAA...GCA-3'\n______________AUGAAU_GUUUC______________AGUCUU_____GCAA____UGCC__UCUC___UUCCU________________CAUUUCU\n______________||||||_|:|||______________|:||||_____:|||____|:||__||||___||||:________________|||||||\n______________UACUUA_CGAAG______________UUAGAA_____UGUU____AUGG__AGAG___AAGGG________________GUAAAGA\n3'-AUU...UUCAU______C_____CAACG_______________CCGUU____AGC_____GU____AAU____________________________AACUU...CAA-5'\n_____________|______________________________________________________________________________________|\n_____________58_____________________________________________________________________________________32\n |
83 -- 89 |
33 -- 39 |
-30.7855 kcal/mol |
7.29289 kcal/mol |
15.7787 kcal/mol |
50 90@LB@ | |@LB@ 5'-CUCAU A UUUUAUAGAUACAA AUAUC AAAA AU C UUCCAUCCCUCGAUAA CAAAA...GCA-3'@LB@ AUGAAU GUUUC AGUCUU GCAA UGCC UCUC UUCCU CAUUUCU@LB@ |||||| |:||| |:|||| :||| |:|| |||| ||||: |||||||@LB@ UACUUA CGAAG UUAGAA UGUU AUGG AGAG AAGGG GUAAAGA@LB@3'-AUU...UUCAU C CAACG CCGUU AGC GU AAU AACUU...CAA-5'@LB@ | |@LB@ 58 32@LB@@LB@@LB@Energy -10.53 kcal/mol@LB@Hybridization Energy -30.7855 kcal/mol@LB@Unfolding Energy - mRNA 7.29289 kcal/mol@LB@Unfolding Energy - sRNA 15.7787 kcal/mol@LB@@LB@Position - mRNA 51 -- 89@LB@Position - sRNA 33 -- 57@LB@Position Seed - mRNA 83 -- 89@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 127 |
0.005895 |
0.1741 |
bsu15640 |
yloA |
-14.75 |
0.037563 |
39 -- 86 |
36 -- 83 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 11238985 17586671; Product type pf: factor' |
|
_____________38__________________________________________________87\n_____________|___________________________________________________|\n5'-UUA...ACGUA__G_____C____A_______CUGU_____________AUGAAA_______CCGCU...AUA-3'\n______________UU_GUUGC__UCU__GUAAAU____UUGU_CCCAUUUC______CUUCAUU\n______________||_|||||__|||__|:|||:____|:||_|||||:||______|::||||\n______________AA_CAACG__AGA__CGUUUG____AGCA_GGGUAGAG______GGGGUAA\n3'-AUU...UACCG__G_____UU___AC______UU______U________AAUAA________AGAAA...CAA-5'\n_____________|___________________________________________________|\n_____________84__________________________________________________35\n |
80 -- 86 |
36 -- 42 |
-30.597 kcal/mol |
5.41607 kcal/mol |
13.7079 kcal/mol |
38 87@LB@ | |@LB@5'-UUA...ACGUA G C A CUGU AUGAAA CCGCU...AUA-3'@LB@ UU GUUGC UCU GUAAAU UUGU CCCAUUUC CUUCAUU@LB@ || ||||| ||| |:|||: |:|| |||||:|| |::||||@LB@ AA CAACG AGA CGUUUG AGCA GGGUAGAG GGGGUAA@LB@3'-AUU...UACCG G UU AC UU U AAUAA AGAAA...CAA-5'@LB@ | |@LB@ 84 35@LB@@LB@@LB@Energy -14.75 kcal/mol@LB@Hybridization Energy -30.597 kcal/mol@LB@Unfolding Energy - mRNA 5.41607 kcal/mol@LB@Unfolding Energy - sRNA 13.7079 kcal/mol@LB@@LB@Position - mRNA 39 -- 86@LB@Position - sRNA 36 -- 83@LB@Position Seed - mRNA 80 -- 86@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 128 |
0.005903 |
0.1741 |
bsu24110 |
yqzF |
-12.53 |
0.106013 |
93 -- 138 |
39 -- 81 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________92_______________________________________________139\n_____________|________________________________________________|\n5'-UAA...UAUUC_________AAUUU___C___CCUA___UA___CGUUUUCU_______ACAAA...UCA-3'\n______________CGUUGCAAU_____GCA_AAC____UGU__CCA________UCUCUUG\n______________|||||||||_____|||_|||____:||__|||________||||||:\n______________GCAACGUUA_____CGU_UUG____GCA__GGU________AGAGAAU\n3'-AUU...CCGAA_________GAAC________UUA____UG__________________AAGGG...CAA-5'\n_____________|________________________________________________|\n_____________82_______________________________________________38\n |
134 -- 140 |
45 -- 51 |
-26.5343 kcal/mol |
8.17713 kcal/mol |
8.56905 kcal/mol |
92 139@LB@ | |@LB@5'-UAA...UAUUC AAUUU C CCUA UA CGUUUUCU ACAAA...UCA-3'@LB@ CGUUGCAAU GCA AAC UGU CCA UCUCUUG@LB@ ||||||||| ||| ||| :|| ||| ||||||:@LB@ GCAACGUUA CGU UUG GCA GGU AGAGAAU@LB@3'-AUU...CCGAA GAAC UUA UG AAGGG...CAA-5'@LB@ | |@LB@ 82 38@LB@@LB@@LB@Energy -12.53 kcal/mol@LB@Hybridization Energy -26.5343 kcal/mol@LB@Unfolding Energy - mRNA 8.17713 kcal/mol@LB@Unfolding Energy - sRNA 8.56905 kcal/mol@LB@@LB@Position - mRNA 93 -- 138@LB@Position - sRNA 39 -- 81@LB@Position Seed - mRNA 134 -- 140@LB@Position Seed - sRNA 45 -- 51@LB@ |
| 129 |
0.005948 |
0.1741 |
bsu13580 |
ykrV |
-12.37 |
0.114104 |
93 -- 132 |
31 -- 61 |
transaminase MtnE |
|
_____________92________________________________________133\n_____________|_________________________________________|\n5'-CAG...AAGAU________AUUU________UAACA_____AAUU_______ACUUU...ACG-3'\n______________AUCGU_CC____CAUCUUUU_____CCCCA____UUUCUUU\n______________|||||_||____|||||:||_____|||||____|||||||\n______________UAGCA_GG____GUAGAGAA_____GGGGU____AAAGAAA\n3'-AUU...UUUGU_____U______________UAA__________________CUUUA...CAA-5'\n_____________|_________________________________________|\n_____________62________________________________________30\n |
126 -- 132 |
31 -- 37 |
-24.5638 kcal/mol |
3.82436 kcal/mol |
10.4555 kcal/mol |
92 133@LB@ | |@LB@5'-CAG...AAGAU AUUU UAACA AAUU ACUUU...ACG-3'@LB@ AUCGU CC CAUCUUUU CCCCA UUUCUUU@LB@ ||||| || |||||:|| ||||| |||||||@LB@ UAGCA GG GUAGAGAA GGGGU AAAGAAA@LB@3'-AUU...UUUGU U UAA CUUUA...CAA-5'@LB@ | |@LB@ 62 30@LB@@LB@@LB@Energy -12.37 kcal/mol@LB@Hybridization Energy -24.5638 kcal/mol@LB@Unfolding Energy - mRNA 3.82436 kcal/mol@LB@Unfolding Energy - sRNA 10.4555 kcal/mol@LB@@LB@Position - mRNA 93 -- 132@LB@Position - sRNA 31 -- 61@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 31 -- 37@LB@ |
| 130 |
0.005995 |
0.1741 |
bsu19660 |
yozD |
-10.24 |
0.285413 |
18 -- 51 |
105 -- 135 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________17_________________________________52\n_____________|__________________________________|\n5'-CAC...CUUUG____UUUU_____U______AUCAAAU_______CGUCC...UGU-3'\n______________AGCG____UUCCA_UAUGAU_______CUUUCUC\n______________|||:____|||||_|||:||_______|||||||\n______________UCGU____AAGGU_AUAUUA_______GAAAGAG\n3'-AUU...AGGCG____UC______________CAACAGU_______UAGAA...CAA-5'\n_____________|__________________________________|\n_____________136________________________________104\n |
45 -- 51 |
105 -- 111 |
-19.2876 kcal/mol |
2.67508 kcal/mol |
6.61557 kcal/mol |
17 52@LB@ | |@LB@5'-CAC...CUUUG UUUU U AUCAAAU CGUCC...UGU-3'@LB@ AGCG UUCCA UAUGAU CUUUCUC@LB@ |||: ||||| |||:|| |||||||@LB@ UCGU AAGGU AUAUUA GAAAGAG@LB@3'-AUU...AGGCG UC CAACAGU UAGAA...CAA-5'@LB@ | |@LB@ 136 104@LB@@LB@@LB@Energy -10.24 kcal/mol@LB@Hybridization Energy -19.2876 kcal/mol@LB@Unfolding Energy - mRNA 2.67508 kcal/mol@LB@Unfolding Energy - sRNA 6.61557 kcal/mol@LB@@LB@Position - mRNA 18 -- 51@LB@Position - sRNA 105 -- 135@LB@Position Seed - mRNA 45 -- 51@LB@Position Seed - sRNA 105 -- 111@LB@ |
