| 125 |
0.005817 |
0.1741 |
bsu26980 |
yraE |
-11.00 |
0.208718 |
35 -- 90 |
112 -- 185 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________34________________________________________________________91\n_____________|_________________________________________________________|\n5'-UAC...UUUGA___AG______________AA_______A____GGA_____________________AGCCA...CUG-3'\n______________ACU___UAUCGACAGG_CA_____AUUG_UCCG_______________GAUGUUGUC\n______________|||___:|||:|||||_||_____||||_||||_______________:||||||||\n______________UGA___GUAGUUGUCC_GU_____UAAC_AGGC_______________UUACAACAG\n3'-AUU...AAAAA___GAA__________U__AACUG_________GUCGUUCAAGGUAUA_________UGAAA...CAA-5'\n_____________|_________________________________________________________|\n_____________186_______________________________________________________111\n |
83 -- 89 |
113 -- 119 |
-30.5681 kcal/mol |
8.54797 kcal/mol |
10.8348 kcal/mol |
34 91@LB@ | |@LB@5'-UAC...UUUGA AG AA A GGA AGCCA...CUG-3'@LB@ ACU UAUCGACAGG CA AUUG UCCG GAUGUUGUC@LB@ ||| :|||:||||| || |||| |||| :||||||||@LB@ UGA GUAGUUGUCC GU UAAC AGGC UUACAACAG@LB@3'-AUU...AAAAA GAA U AACUG GUCGUUCAAGGUAUA UGAAA...CAA-5'@LB@ | |@LB@ 186 111@LB@@LB@@LB@Energy -11.00 kcal/mol@LB@Hybridization Energy -30.5681 kcal/mol@LB@Unfolding Energy - mRNA 8.54797 kcal/mol@LB@Unfolding Energy - sRNA 10.8348 kcal/mol@LB@@LB@Position - mRNA 35 -- 90@LB@Position - sRNA 112 -- 185@LB@Position Seed - mRNA 83 -- 89@LB@Position Seed - sRNA 113 -- 119@LB@ |
| 120 |
0.005453 |
0.1741 |
bsu38750 |
cydB |
-16.17 |
0.018868 |
36 -- 121 |
37 -- 136 |
cytochrome d ubiquinol oxidase subunit 2 |
|
_____________35____________________________________________________________________________________________________________122\n_____________|_____________________________________________________________________________________________________________|\n5'-AAU...CGUAA___________________________C__AA_____GCA_____U______UAC___AU__________________________________U________A_____GAGAU...CCC-3'\n______________CAGCGGGUUCC________UUGUUAUU_UC__UCAUC___UUCGG_GUGAUG___AUG____________UCUUGGU__GCA__C_UGCUUGUC_UCUUGUUU_UCCGU\n______________||||::|||||________||||:|:|_||__|||||___|||::_||:|||___|||____________||||||:__:||__|_|:|:::||_||||:||:_:||:|\n______________GUCGUUCAAGG________AACAGUGA_AG__AGUAG___AAGUU_CAUUAC___UAC____________AGAACCG__UGU__G_AUGGGUAG_AGAAUAAG_GGGUA\n3'-AUU...CAGGC___________UAUAUUAC________A_________________U______U_____CGAAGCAACGUU_______UU___UA_C_______________________AAGAA...CAA-5'\n_____________|_____________________________________________________________________________________________________________|\n_____________137___________________________________________________________________________________________________________36\n |
109 -- 115 |
42 -- 48 |
-60.5971 kcal/mol |
23.6183 kcal/mol |
24.7809 kcal/mol |
35 122@LB@ | |@LB@5'-AAU...CGUAA C AA GCA U UAC AU U A GAGAU...CCC-3'@LB@ CAGCGGGUUCC UUGUUAUU UC UCAUC UUCGG GUGAUG AUG UCUUGGU GCA C UGCUUGUC UCUUGUUU UCCGU@LB@ ||||::||||| ||||:|:| || ||||| |||:: ||:||| ||| ||||||: :|| | |:|:::|| ||||:||: :||:|@LB@ GUCGUUCAAGG AACAGUGA AG AGUAG AAGUU CAUUAC UAC AGAACCG UGU G AUGGGUAG AGAAUAAG GGGUA@LB@3'-AUU...CAGGC UAUAUUAC A U U CGAAGCAACGUU UU UA C AAGAA...CAA-5'@LB@ | |@LB@ 137 36@LB@@LB@@LB@Energy -16.17 kcal/mol@LB@Hybridization Energy -60.5971 kcal/mol@LB@Unfolding Energy - mRNA 23.6183 kcal/mol@LB@Unfolding Energy - sRNA 24.7809 kcal/mol@LB@@LB@Position - mRNA 36 -- 121@LB@Position - sRNA 37 -- 136@LB@Position Seed - mRNA 109 -- 115@LB@Position Seed - sRNA 42 -- 48@LB@ |
| 127 |
0.005895 |
0.1741 |
bsu15640 |
yloA |
-14.75 |
0.037563 |
39 -- 86 |
36 -- 83 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 11238985 17586671; Product type pf: factor' |
|
_____________38__________________________________________________87\n_____________|___________________________________________________|\n5'-UUA...ACGUA__G_____C____A_______CUGU_____________AUGAAA_______CCGCU...AUA-3'\n______________UU_GUUGC__UCU__GUAAAU____UUGU_CCCAUUUC______CUUCAUU\n______________||_|||||__|||__|:|||:____|:||_|||||:||______|::||||\n______________AA_CAACG__AGA__CGUUUG____AGCA_GGGUAGAG______GGGGUAA\n3'-AUU...UACCG__G_____UU___AC______UU______U________AAUAA________AGAAA...CAA-5'\n_____________|___________________________________________________|\n_____________84__________________________________________________35\n |
80 -- 86 |
36 -- 42 |
-30.597 kcal/mol |
5.41607 kcal/mol |
13.7079 kcal/mol |
38 87@LB@ | |@LB@5'-UUA...ACGUA G C A CUGU AUGAAA CCGCU...AUA-3'@LB@ UU GUUGC UCU GUAAAU UUGU CCCAUUUC CUUCAUU@LB@ || ||||| ||| |:|||: |:|| |||||:|| |::||||@LB@ AA CAACG AGA CGUUUG AGCA GGGUAGAG GGGGUAA@LB@3'-AUU...UACCG G UU AC UU U AAUAA AGAAA...CAA-5'@LB@ | |@LB@ 84 35@LB@@LB@@LB@Energy -14.75 kcal/mol@LB@Hybridization Energy -30.597 kcal/mol@LB@Unfolding Energy - mRNA 5.41607 kcal/mol@LB@Unfolding Energy - sRNA 13.7079 kcal/mol@LB@@LB@Position - mRNA 39 -- 86@LB@Position - sRNA 36 -- 83@LB@Position Seed - mRNA 80 -- 86@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 133 |
0.006226 |
0.1741 |
bsu14430 |
ykpA |
-10.78 |
0.228396 |
48 -- 105 |
75 -- 129 |
ABC transporter ATP-binding protein |
|
_____________47____________106\n_____________|_____________|\n5'-GUA...AAGUU_____________AAUAU...UUG-3'\n______________AUUUGAAGAUGUU\n______________|||||||:|:|:|\n______________UAAACUUUUGCGA\n3'-AUU...AACUU_____________AUUAA...CAA-5'\n_____________|_____________|\n_____________130___________74\n |
254 -- 260 |
15 -- 21 |
-11.9489 kcal/mol |
0.844332 kcal/mol |
3.96844 kcal/mol |
47 106@LB@ | |@LB@5'-GUA...AAGUU AAUAU...UUG-3'@LB@ AUUUGAAGAUGUU@LB@ |||||||:|:|:|@LB@ UAAACUUUUGCGA@LB@3'-AUU...AACUU AUUAA...CAA-5'@LB@ | |@LB@ 130 74@LB@@LB@@LB@Energy -10.78 kcal/mol@LB@Hybridization Energy -11.9489 kcal/mol@LB@Unfolding Energy - mRNA 0.844332 kcal/mol@LB@Unfolding Energy - sRNA 3.96844 kcal/mol@LB@@LB@Position - mRNA 48 -- 105@LB@Position - sRNA 75 -- 129@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 126 |
0.005872 |
0.1741 |
bsu36510 |
amtB |
-10.53 |
0.253946 |
51 -- 89 |
33 -- 57 |
ammonium transporter NrgA |
|
_____________50_____________________________________________________________________________________90\n_____________|______________________________________________________________________________________|\n______5'-CUCAU______A_____UUUUAUAGAUACAA______AUAUC____AAAA____AU____C_______UUCCAUCCCUCGAUAA_______CAAAA...GCA-3'\n______________AUGAAU_GUUUC______________AGUCUU_____GCAA____UGCC__UCUC___UUCCU________________CAUUUCU\n______________||||||_|:|||______________|:||||_____:|||____|:||__||||___||||:________________|||||||\n______________UACUUA_CGAAG______________UUAGAA_____UGUU____AUGG__AGAG___AAGGG________________GUAAAGA\n3'-AUU...UUCAU______C_____CAACG_______________CCGUU____AGC_____GU____AAU____________________________AACUU...CAA-5'\n_____________|______________________________________________________________________________________|\n_____________58_____________________________________________________________________________________32\n |
83 -- 89 |
33 -- 39 |
-30.7855 kcal/mol |
7.29289 kcal/mol |
15.7787 kcal/mol |
50 90@LB@ | |@LB@ 5'-CUCAU A UUUUAUAGAUACAA AUAUC AAAA AU C UUCCAUCCCUCGAUAA CAAAA...GCA-3'@LB@ AUGAAU GUUUC AGUCUU GCAA UGCC UCUC UUCCU CAUUUCU@LB@ |||||| |:||| |:|||| :||| |:|| |||| ||||: |||||||@LB@ UACUUA CGAAG UUAGAA UGUU AUGG AGAG AAGGG GUAAAGA@LB@3'-AUU...UUCAU C CAACG CCGUU AGC GU AAU AACUU...CAA-5'@LB@ | |@LB@ 58 32@LB@@LB@@LB@Energy -10.53 kcal/mol@LB@Hybridization Energy -30.7855 kcal/mol@LB@Unfolding Energy - mRNA 7.29289 kcal/mol@LB@Unfolding Energy - sRNA 15.7787 kcal/mol@LB@@LB@Position - mRNA 51 -- 89@LB@Position - sRNA 33 -- 57@LB@Position Seed - mRNA 83 -- 89@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 117 |
0.005342 |
0.1741 |
bsu33050 |
gerAA |
-9.34 |
0.402150 |
52 -- 63 |
38 -- 49 |
spore germination protein A1 |
|
_____________51___________64\n_____________|____________|\n5'-UAA...UUUCU____________GCUAG...UGC-3'\n______________UCUUGUUUUCCA\n______________||||:||::|||\n______________AGAAUAAGGGGU\n3'-AUU...GGUAG____________AAAGA...CAA-5'\n_____________|____________|\n_____________50___________37\n |
57 -- 63 |
38 -- 44 |
-12.8318 kcal/mol |
1.31908 kcal/mol |
4.13482 kcal/mol |
51 64@LB@ | |@LB@5'-UAA...UUUCU GCUAG...UGC-3'@LB@ UCUUGUUUUCCA@LB@ ||||:||::|||@LB@ AGAAUAAGGGGU@LB@3'-AUU...GGUAG AAAGA...CAA-5'@LB@ | |@LB@ 50 37@LB@@LB@@LB@Energy -9.34 kcal/mol@LB@Hybridization Energy -12.8318 kcal/mol@LB@Unfolding Energy - mRNA 1.31908 kcal/mol@LB@Unfolding Energy - sRNA 4.13482 kcal/mol@LB@@LB@Position - mRNA 52 -- 63@LB@Position - sRNA 38 -- 49@LB@Position Seed - mRNA 57 -- 63@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 140 |
0.006451 |
0.1741 |
bsu29090 |
polA |
-11.51 |
0.167440 |
58 -- 84 |
30 -- 56 |
DNA polymerase I |
|
_____________57________________85\n_____________|_________________|\n5'-AUG...CGGGA________G________GUCCU...AUA-3'\n______________ACCUAUUU__UGUUCCC\n______________|||:||:|__|:|||||\n______________UGGGUAGA__AUAAGGG\n3'-AUU...UAGCA________GA_______GUAAA...CAA-5'\n_____________|_________________|\n_____________57________________29\n |
67 -- 73 |
40 -- 46 |
-17.5976 kcal/mol |
2.41953 kcal/mol |
7.22212 kcal/mol |
57 85@LB@ | |@LB@5'-AUG...CGGGA G GUCCU...AUA-3'@LB@ ACCUAUUU UGUUCCC@LB@ |||:||:| |:|||||@LB@ UGGGUAGA AUAAGGG@LB@3'-AUU...UAGCA GA GUAAA...CAA-5'@LB@ | |@LB@ 57 29@LB@@LB@@LB@Energy -11.51 kcal/mol@LB@Hybridization Energy -17.5976 kcal/mol@LB@Unfolding Energy - mRNA 2.41953 kcal/mol@LB@Unfolding Energy - sRNA 7.22212 kcal/mol@LB@@LB@Position - mRNA 58 -- 84@LB@Position - sRNA 30 -- 56@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 144 |
0.006712 |
0.1741 |
bsu25040 |
yqgB |
-19.28 |
0.004052 |
60 -- 160 |
55 -- 168 |
Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; PubMedId: 15294815; Product type ph: phenotype |
|
_____________59_____________________________________________________161\n_____________|______________________________________________________|\n5'-UAA...AAAAC__C_______AACA__CAAU______A_______AG___CCAUCC_________ACUCU...UUU-3'\n______________CG_UGCAAUC____GC____ACAA_C_UAUCCAU__CUU______UCCCAUUUU\n______________||_|||||||____||____||||_|_||:||||__|||______:|||||||:\n______________GC_ACGUUAG____CG____UGUU_G_AUGGGUA__GAA______GGGGUAAAG\n3'-AUU...CCGAA__A_______AAC___UU______A_C_______GA___UAA____________AAACU...CAA-5'\n_____________|______________________________________________________|\n_____________169____________________________________________________54\n |
105 -- 111 |
34 -- 40 |
-33.5165 kcal/mol |
5.00067 kcal/mol |
13.93 kcal/mol |
59 161@LB@ | |@LB@5'-UAA...AAAAC C AACA CAAU A AG CCAUCC ACUCU...UUU-3'@LB@ CG UGCAAUC GC ACAA C UAUCCAU CUU UCCCAUUUU@LB@ || ||||||| || |||| | ||:|||| ||| :|||||||:@LB@ GC ACGUUAG CG UGUU G AUGGGUA GAA GGGGUAAAG@LB@3'-AUU...CCGAA A AAC UU A C GA UAA AAACU...CAA-5'@LB@ | |@LB@ 169 54@LB@@LB@@LB@Energy -19.28 kcal/mol@LB@Hybridization Energy -33.5165 kcal/mol@LB@Unfolding Energy - mRNA 5.00067 kcal/mol@LB@Unfolding Energy - sRNA 13.93 kcal/mol@LB@@LB@Position - mRNA 60 -- 160@LB@Position - sRNA 55 -- 168@LB@Position Seed - mRNA 105 -- 111@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 121 |
0.005491 |
0.1741 |
bsu40190 |
fbp |
-16.50 |
0.016068 |
67 -- 83 |
53 -- 69 |
fructose-1 6-bisphosphatase |
|
_____________66________________84\n_____________|_________________|\n5'-AAA...AAUGA___________U_____UAAAC...GCA-3'\n______________GGCAAACAAUC_UACCC\n______________|||||||||||_|||||\n______________CCGUUUGUUAG_AUGGG\n3'-AUU...UAGAA___________C_____UAGAG...CAA-5'\n_____________|_________________|\n_____________70________________52\n |
71 -- 77 |
59 -- 65 |
-25.6398 kcal/mol |
2.55798 kcal/mol |
7.79863 kcal/mol |
66 84@LB@ | |@LB@5'-AAA...AAUGA U UAAAC...GCA-3'@LB@ GGCAAACAAUC UACCC@LB@ ||||||||||| |||||@LB@ CCGUUUGUUAG AUGGG@LB@3'-AUU...UAGAA C UAGAG...CAA-5'@LB@ | |@LB@ 70 52@LB@@LB@@LB@Energy -16.50 kcal/mol@LB@Hybridization Energy -25.6398 kcal/mol@LB@Unfolding Energy - mRNA 2.55798 kcal/mol@LB@Unfolding Energy - sRNA 7.79863 kcal/mol@LB@@LB@Position - mRNA 67 -- 83@LB@Position - sRNA 53 -- 69@LB@Position Seed - mRNA 71 -- 77@LB@Position Seed - sRNA 59 -- 65@LB@ |
| 131 |
0.006003 |
0.1741 |
bsu37510 |
pbpG |
-10.90 |
0.216982 |
70 -- 106 |
84 -- 108 |
penicillin-binding protein 2D |
|
_____________69____________________________________107\n_____________|_____________________________________|\n5'-UGU...UGAAA___________CCUAAGGAAA_____UAGA_______GGGGA...UGU-3'\n______________UCUCAUUUUCA__________AAGUA____GGAUGGC\n______________||||||:||||__________|||||____|:|||||\n______________AGAGUAGAAGU__________UUCAU____CUUACCG\n3'-AUU...GUGAA__________________________UA_________AAGCA...CAA-5'\n_____________|_____________________________________|\n_____________109___________________________________83\n |
100 -- 106 |
84 -- 90 |
-24.2284 kcal/mol |
7.40636 kcal/mol |
9.09036 kcal/mol |
69 107@LB@ | |@LB@5'-UGU...UGAAA CCUAAGGAAA UAGA GGGGA...UGU-3'@LB@ UCUCAUUUUCA AAGUA GGAUGGC@LB@ ||||||:|||| ||||| |:|||||@LB@ AGAGUAGAAGU UUCAU CUUACCG@LB@3'-AUU...GUGAA UA AAGCA...CAA-5'@LB@ | |@LB@ 109 83@LB@@LB@@LB@Energy -10.90 kcal/mol@LB@Hybridization Energy -24.2284 kcal/mol@LB@Unfolding Energy - mRNA 7.40636 kcal/mol@LB@Unfolding Energy - sRNA 9.09036 kcal/mol@LB@@LB@Position - mRNA 70 -- 106@LB@Position - sRNA 84 -- 108@LB@Position Seed - mRNA 100 -- 106@LB@Position Seed - sRNA 84 -- 90@LB@ |
