Input and runtime details for job 6571257 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 6571257 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:18:03 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:20:22 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:21:19 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:22:25 CET 2020
	Job Completed		@ Mon Jan 27 14:22:35 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6571257&toolName=IntaRNA ( 30 days expiry )

Description of the job

Spot42 - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b0718	20	33	25	31	3.34	Spot42	1	14	3	9	5.33	-10.35	-19.02	-1.06679	CUUUUGUAUCCUGC&GUAGGGUACAGAGG	((((((((((((((&))))))))))))))	GCUGGAUUAUAAAUAAGUAUUAUUUUGGCGGCAUCAUCGCCGCCUUUCAUAAAAUAUUUAUCAGGUAUGGACACCGUGAAUAUUUAUCGACUCUCUUUUGUAUCCUGCCUGGUCAUGGCGAUGCCUUGCGCAAUGGCGGUCGAAUUCAAU	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0124179	0.9238006	ybgQ	946537	putative outer membrane protein
b4659	25	32	25	31	0.8	Spot42	49	56	50	56	0.3	-8.71	-9.81	-0.897755	CCGGUUAC&GUAAUCGG	((((((((&))))))))	GAAUGAGCGAUUUUUGAAAGUAUAUUUAUUCAGAACGCGCAUCAUGAGUUUUUAACUCAAUGCGAGGCUAUUACCAUGAAAGUAUCAGUUCCAGGCAUGCCGGUUACACUUUUAAAUAUGAGCAAGAACGAUAUUUAUAAGAUGGUGAGC	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0313324	0.9238006	yabP	944776	pseudogene pentapeptide repeats-containing
b1343	21	34	28	34	1.43	Spot42	11	24	11	17	7	-8.89	-17.32	-0.916308	GAAUGUUUUGCCUC&GAGGUAAGAUGUUC	((((((((((((((&))))))))))))))	AUUGUCGCAUCGUGAGCGUAGAUCUGCGAGGAUACGCGCCUGCUAACUUUGCGUCGAUGACCACGAGAAUAGAUUGUGACCGCUUUUUCUACCCUGAAUGUUUUGCCUCCCGCCCAACUCACGAACCUUAAUGAGUUGGGUUAUUUAACC	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0283265	0.9238006	dbpA	947153	ATP-dependent RNA helicase specific for 23S rRNA
b1385	21	37	31	37	2.01	Spot42	55	71	55	61	6.06	-11.57	-19.64	-1.19254	AGUAUUAAGCCAGGUCC&GGAUUUGGCUGAAUAUU	((((((.((((((((((&)))))))))).))))))	GUCACAGCGAAAAAAGUGACUUUUCUUGUCGCUGCGUACACUGAAAUCACACUGGGUAAAUAAUAAGGAAAAGUGAUGACAGAGCCGCAUGUAGCAGUAUUAAGCCAGGUCCAACAGUUUCUCGAUCGUCAACACGGUCUUUAUAUUGAU	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.006193	0.9238006	feaB	945933	phenylacetaldehyde dehydrogenase
b3465	27	38	31	37	4.39	Spot42	54	65	55	61	6.13	-8.75	-19.27	-0.901877	CGGCCAAAUCCG&CGGAUUUGGCUG	((((((((((((&))))))))))))	AAAUAGUCUAUCACUUAACUUAAUUCACAUCACCGCCUGCAAGUAUGUGUUAUCUGGCGGAUUGAGCAAUUUAUCAUGAAAAAACCGAAUCAUUCCGGCAGCGGCCAAAUCCGCAUUAUUGGCGGGCAGUGGCGAGGCCGUAAACUCCCG	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0306386	0.9238006	rsmD	947977	16S rRNA m(2)G966 methyltransferase SAM-dependent
b2962	26	68	33	39	15.68	Spot42	4	57	44	50	12.67	-10.78	-39.13	-1.11111	UCCUGCAACGUGAAGCAGAAGGUCAGGAUUUUCAGCUGUACCC&GGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGA	(((....((((((((.(((((((((((((......((((((((&))))))))..............)))))...)))))))).))))))))....)))	CCCGUGGAGCGUUUGUUACAGCAGUUACGCACUGGCGCGCCGGUUUAGCGCGUGAGUCGAUAAAGAGGAUGAUUUAUGAGCAGAACGAUUUUUUGUACUUUCCUGCAACGUGAAGCAGAAGGUCAGGAUUUUCAGCUGUACCCCGGCGAG	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.009723	0.9238006	yggX	947461	oxidative damage protective factor for iron-sulfur proteins
b0559	38	49	38	44	2.17	Spot42	46	58	52	58	1.84	-9.04	-13.05	-0.931768	AUCUGAUUCGUG&CACGUAAUCGGAU	((((((((((((&)))).))))))))	CUCAAACCUUCAUACAGAAAGAUUUGAAGUAAUAUUUUAACCGCUAGAUGAAGAGCAAGCGCAUGGAGCGACAAAAUGAAUAAAGAACAAUCUGCUGAUGAUCCCUCCGUGGAUCUGAUUCGUGUAAAAAAUAUGCUUAAUAGCACCAUU	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0260389	0.9238006	ybcW	945175	DLP12 prophage uncharacterized protein
b0954	28	49	40	46	5.11	Spot42	9	31	12	18	7.95	-9.87	-22.93	-1.01732	AAAGAAGACCUUCUUGCCUCUG&CAGAGGUAAGAUGUUCUAUCUUU	((((((((...(((((((((((&)))))))))))...))).)))))	GAACUGAUCGGACUUGUUCAGCGUACACGUGUUAGCUAUCCUGCGUGCUUCAAUAAAAUAAGGCUUACAGAGAACAUGGUAGAUAAACGCGAAUCCUAUACAAAAGAAGACCUUCUUGCCUCUGGUCGCGGUGAACUGUUUGGCGCUAAA	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0163036	0.9238006	fabA	945568	beta-hydroxydecanoyl thioester dehydrase
b0705	41	48	41	47	1.46	Spot42	49	56	50	56	0.3	-9.4	-11.16	-0.968874	CCGAUUAC&GUAAUCGG	((((((((&))))))))	UAUUCCUUAUUACCUAAAGCUGUUUUUCAUAGCUUAUACAUGAUCAAAUACUCCUUACAUAAAUAAGGUGAACAAAUGGAACUUAAAAAAUUGAUGGAACAUAUUUCUAUUAUUCCCGAUUACAGAUAAGCCUGGAAAGUAGAGCAUAAA	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0212627	0.9238006	ybfL	947075	pseudogene DDE domain transposase familyputative factor Not classified putative receptor protein
b3092	6	69	55	61	11.46	Spot42	1	57	5	11	12.67	-10.14	-34.27	-1.04515	UCCGUUUAUGACUGAAGAUUUCCUGUUAGAUACCGAAUUUGCCCGCCGUCUGUAUCACGACUAC&GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGA	((((.(((((..(((((.....(((..(((((....(((((((.....((((((((....((((&))))))))))))))))).))....)))))..)))........)))))))))).))))	CUGGAAAUUGGUGUGAUAACUUUGUCAGCAUCGCACCAUAAGCAAGCUAGCUCACUCGUUGAGAGGAAGACGAAAAUGACUCCGUUUAUGACUGAAGAUUUCCUGUUAGAUACCGAAUUUGCCCGCCGUCUGUAUCACGACUACGCAAAA	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0139903	0.9238006	uxaC	947599	uronate isomerase

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching