Input and runtime details for job 4596356 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 4596356 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:10:48 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:13:48 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:14:50 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:16:49 CET 2020
	Job Completed		@ Mon Jan 27 14:17:02 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=4596356&toolName=IntaRNA ( 30 days expiry )

Description of the job

FnrS - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b0802	-20	-13	-19	-13	2.26	FnrS	1	8	1	7	2.36	-10.42	-15.04	-1.06168	UCACCUGC&GCAGGUGA	((((((((&))))))))	CGUAAAAAAACGUGAUACUCAUCACAUCGACGAAACAACGUCACUUAUACAAAAAUCACCUGCGAGAGAUUAAUUAUGAAAACUAUCAAUACUGUUGUUGCUGCUAUGGCUCUUUCAACUCUGUCAUUUGGCGUGUUCGCGGCGGAACCG	GCAGGUGAAUGCAACGUCAAGCGAUGGGCGUUGCGCUCCAUAUUGUCUUACUUCCUUUUUUGAAUUACUGCAUAGCACAAUUGAUUCGUACGACGCCGACUUUGAUGAGUCGGCUUUUUUUU	0.0196176	0.9422174	ybiJ	945433	DUF1471 family putative periplasmic protein
b4020	15	22	16	22	1.63	FnrS	1	8	1	7	2.36	-10.42	-14.41	-1.06168	UCACCUGC&GCAGGUGA	((((((((&))))))))	CCAGUCAGCGUGUUUCCGGUCCUUUGGUCAUUCAAUUGCUUCAGGAACAUAUAACGAUAAGGAGAACCUGAACUCGUGUUAACGCUGCUUCACCUGCUUUCUGCCGUCGCCCUGCUGGUCUGGGGGACUCAUAUUGUUCGAACCGGCGUA	GCAGGUGAAUGCAACGUCAAGCGAUGGGCGUUGCGCUCCAUAUUGUCUUACUUCCUUUUUUGAAUUACUGCAUAGCACAAUUGAUUCGUACGACGCCGACUUUGAUGAGUCGGCUUUUUUUU	0.0196176	0.9422174	yjbB	948521	putative Na+/Pi-cotransporter
b4602	-44	-10	-16	-10	7.78	FnrS	53	92	53	59	4.83	-10.3	-22.91	-1.04945	CGUAAUAUCACUUUUUGUGUGGUAAUAAAAAAGGA&UCCUUUUUUGAAUUACUGCAUAGCACAAUUGAUUCGUACG	((((..((((.....((((((((((((((((((((&)))))))))..))))))))))).......))))...))))	GGAGAUUUUUUCAUCACAGUGUGUAAAAAUGCGUAAUAUCACUUUUUGUGUGGUAAUAAAAAAGGAGAAAACUUGAUGAGCACCGACCUUAAAUUUUCACUGGUAACAACGAUUAUCGUCCUCGGUUUGAUCGUAGCCGUGGGUUUGACU	GCAGGUGAAUGCAACGUCAAGCGAUGGGCGUUGCGCUCCAUAUUGUCUUACUUCCUUUUUUGAAUUACUGCAUAGCACAAUUGAUUCGUACGACGCCGACUUUGAUGAGUCGGCUUUUUUUU	0.0212222	0.9422174	ynhF	5061505	stress response membrane
b0889	-28	-4	-11	-5	2.54	FnrS	41	61	42	48	2.37	-10.3	-15.21	-1.04945	AGGGAAGGAAUACAGAGAGACAAUA&UAUUGUCUUACUUCCUUUUUU	(((((((((....((..((((((((&))))))))..)))))))))))	GACGGGUAGCGACUCUGAACAGUGAUGUUUCAGGGUCAGACAGGAGUAGGGAAGGAAUACAGAGAGACAAUAAUAAUGGUAGAUAGCAAGAAGCGCCCUGGCAAAGAUCUCGACCGUAUCGAUCGUAACAUUCUUAAUGAGUUGCAAAAG	GCAGGUGAAUGCAACGUCAAGCGAUGGGCGUUGCGCUCCAUAUUGUCUUACUUCCUUUUUUGAAUUACUGCAUAGCACAAUUGAUUCGUACGACGCCGACUUUGAUGAGUCGGCUUUUUUUU	0.0212222	0.9422174	lrp	949051	leucine-responsive global transcriptional regulator
b0006	15	23	17	23	2.78	FnrS	1	9	1	7	2.36	-10.3	-15.44	-1.04945	UUCACCUGC&GCAGGUGAA	(((((((((&)))))))))	GAGUAAUCACAACUAUCGAUCAACUCAUUCUCAUUUUUUGCUAAAGUCGGCAUAAAUUUCCUGCAAGGACUGGAUAUGCUGAUUCUUAUUUCACCUGCGAAAACGCUUGAUUACCAAAGCCCGUUGACCACCACGCGCUAUACGCUGCCG	GCAGGUGAAUGCAACGUCAAGCGAUGGGCGUUGCGCUCCAUAUUGUCUUACUUCCUUUUUUGAAUUACUGCAUAGCACAAUUGAUUCGUACGACGCCGACUUUGAUGAGUCGGCUUUUUUUU	0.0212222	0.9422174	yaaA	944749	peroxide resistance protein lowers intracellular iron
b3195	-12	7	1	7	1.09	FnrS	35	54	35	41	3.99	-10.22	-15.3	-1.0413	GAAGGGUGAAUUAUGGAGC&GCUCCAUAUUGUCUUACUUC	((((...((..((((((((&))))))))...))...))))	UUCUGUAAAUAUGUUGGGUUCAAGGUUAAAUUGAGCGCCAUGCUUAGAAAAUCAACGCAAGACGAAGGGUGAAUUAUGGAGCAGUCUGUGGCGAAUUUAGUCGAUAUGCGCGAUGUCAGUUUUACGCGUGGCAAUCGCUGCAUCUUCGAU	GCAGGUGAAUGCAACGUCAAGCGAUGGGCGUUGCGCUCCAUAUUGUCUUACUUCCUUUUUUGAAUUACUGCAUAGCACAAUUGAUUCGUACGACGCCGACUUUGAUGAGUCGGCUUUUUUUU	0.0223567	0.9422174	mlaF	947729	ABC transporter maintaining OM lipid asymmetry ATP-binding protein
b2592	55	62	56	62	0.19	FnrS	1	8	1	7	2.36	-10.21	-12.76	-1.04028	UCACUUGC&GCAGGUGA	((((((((&))))))))	UCAAUAACCUUGAAUAAUUGAGGGAUGACCUCAUUUAAUCUCCAGUAGCAACUUUGAUCCGUUAUGGGAGGAGUUAUGCGUCUGGAUCGUCUUACUAAUAAAUUCCAGCUUGCUCUUGCCGAUGCCCAAUCACUUGCACUCGGGCACGAC	GCAGGUGAAUGCAACGUCAAGCGAUGGGCGUUGCGCUCCAUAUUGUCUUACUUCCUUUUUUGAAUUACUGCAUAGCACAAUUGAUUCGUACGACGCCGACUUUGAUGAGUCGGCUUUUUUUU	0.0225023	0.9422174	clpB	947077	protein disaggregation chaperone
b1817	-75	-47	-70	-64	4.53	FnrS	57	88	77	83	4.63	-10.21	-19.37	-1.04028	CGAUUCGAUUGUGGACGACGAUUCAAAAA&UUUUUGAAUUACUGCAUAGCACAAUUGAUUCG	(((.(((((((((.......(((((((((&)))))))))..........))))))))).)))	CGAUUCGAUUGUGGACGACGAUUCAAAAAUACAUCUGGCACGUUGAGGUGUUAACGAUAAUAAAGGAGGUAGCAAGUGACCAUUGCUAUUGUUAUAGGCACACAUGGUUGGGCUGCAGAGCAGUUGCUUAAAACGGCAGAAAUGCUGUUA	GCAGGUGAAUGCAACGUCAAGCGAUGGGCGUUGCGCUCCAUAUUGUCUUACUUCCUUUUUUGAAUUACUGCAUAGCACAAUUGAUUCGUACGACGCCGACUUUGAUGAGUCGGCUUUUUUUU	0.0225023	0.9422174	manX	946334	fused mannose-specific PTS enzymes: IIA component/IIB component
b2101	-13	4	-7	-1	2.52	FnrS	38	56	43	49	3.52	-10.2	-16.24	-1.03926	AGGAAAAAGACAAAUGG&CCAUAUUGUCUUACUUCCU	(((((.(((((((((((&)))).)))))))..)))))	GAUGGGAUUAAAGAAACGCCUUAAAUAAGAAGCAGUGAUAUACUCCCACCAGACUCCCCGGCAGGAAAAAGACAAAUGGAACAAGCGCAUACCCAGCUGAUUGCCCAACUGAACGAGCGUAUUUUAGCGGCGGAUAACACGCCGCUUUAU	GCAGGUGAAUGCAACGUCAAGCGAUGGGCGUUGCGCUCCAUAUUGUCUUACUUCCUUUUUUGAAUUACUGCAUAGCACAAUUGAUUCGUACGACGCCGACUUUGAUGAGUCGGCUUUUUUUU	0.0226488	0.9422174	yegW	946639	putative DNA-binding transcriptional regulator
b3892	-16	-1	-9	-3	3.18	FnrS	31	49	33	39	9.51	-10.14	-22.83	-1.03315	AAGACGAGGAGCGCAA&UUGCGCUCCAUAUUGUCUU	((((((((((((((((&)))))))))...)))))))	CUACGUGGGUGUCGGUCCGAACCGUGCGGAUGAGGAAGAGAAUAAUCUGCACGAAGAGAAAGACGAGGAGCGCAAAUGAAACGACGUGACACCAUCGUGCGCUACACCGCGCCGGAACGUAUCAACCACUGGAUCACCGCCUUCUGCUUC	GCAGGUGAAUGCAACGUCAAGCGAUGGGCGUUGCGCUCCAUAUUGUCUUACUUCCUUUUUUGAAUUACUGCAUAGCACAAUUGAUUCGUACGACGCCGACUUUGAUGAGUCGGCUUUUUUUU	0.0235457	0.9422174	fdoI	948383	formate dehydrogenase-O cytochrome b556 subunit

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching