Input and runtime details for job 6571257 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 6571257 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:18:03 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:20:22 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:21:19 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:22:25 CET 2020
	Job Completed		@ Mon Jan 27 14:22:35 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6571257&toolName=IntaRNA ( 30 days expiry )

Description of the job

Spot42 - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b3139	13	19	13	19	0.4	Spot42	1	7	1	7	2.01	-9.42	-11.83	-0.970936	ACCCUAC&GUAGGGU	(((((((&)))))))	CGUCCCUGGCGAUCAAAAAGAACAAAUCCCUGACUAAAUCUAAAAUCGCCUUAAUAUUGGUUUGAGGUAAUAAAAAUGCAUGAAAUAACCCUACUUCAGGGAUUAUCCCUGGCGGCGUUAGUUUUUGUUCUGGGGAUUGAUUUUUGGCUG	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0210242	0.9238006	agaC	947652	N-acetylgalactosamine-specific enzyme IIC component of PTS
b2319	3	19	13	19	6.68	Spot42	20	36	20	26	4.78	-9.12	-20.58	-0.940014	GUCUGAAGGCUGGAACA&UGUUCUAUCUUUCAGAC	(((((((((.(((((((&))))))).)))))))))	GGCACGUUAAUCUCUUCUUCAUGCUCUCUGCUGUAACAUUGGCAGGGAGCUUUGCUAUUUCUGGAGUAAACCACCAUGUCUGAAGGCUGGAACAUUGCCGUCCUGGGCGCAACUGGCGCUGUGGGCGAAGCCCUGCUUGAAACGCUGGCU	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0248939	0.9238006	usg	946797	putative semialdehyde dehydrogenase
b2582	6	19	13	19	2.49	Spot42	4	16	4	10	6.57	-9.03	-18.09	-0.930738	UACCGUUUGUACCC&GGGUACAGAGGUA	((((.(((((((((&)))))))))))))	CCGAUAAUAUGUAACAUAUUAGAAACAUACCGGCGUCGUUGCCGAUAAGUCUCCUUACUCAUCCCGAGGUUAGUUAUGAAUACCGUUUGUACCCAUUGUCAGGCCAUCAAUCGCAUUCCCGACGAUCGGAUCGAAGAUGCGGCAAAAUGC	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0261856	0.9238006	trxC	947062	thioredoxin 2
b1862	13	20	14	20	1.73	Spot42	1	8	1	7	2.09	-9.04	-12.86	-0.931768	UACCCUGC&GUAGGGUA	((((((((&))))))))	UACAUUGCGCCUCCUGAAAAAAUAAAUUUUUUAUGCUAUUACGUAUAUUUGUAUCUAUUUCAAUGGAAUGACAACGUGAAUAUUAAUUACCCUGCUGAAUAUGAAAUUGGAGAUAUCGUCUUUACAUGUAUAGGUGCUGCCUUAUUUGGU	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0260389	0.9238006	yebB	946377	DUF830 family protein
b0774	9	28	16	22	4.03	Spot42	4	24	12	18	7.99	-9.09	-21.11	-0.936922	GGACGAUCUUGCCUUUGACC&GGGUACAGAGGUAAGAUGUUC	((((.((((((((((((.((&))...))))))))))))))))	AAAACGUGUUUUUUGUUGUUAAUUCGGUGUAGACUUGUAAACCUAAAUCUUUUCAAUUUGGUUUACAAGUCGAUUAUGACAACGGACGAUCUUGCCUUUGACCAACGCCAUAUCUGGCACCCAUACACAUCCAUGACCUCCCCUCUGCCG	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0253174	0.9238006	bioA	945376	78-diaminopelargonic acid synthase PLP-dependent
b3664	17	32	17	23	7.49	Spot42	40	56	50	56	3.32	-10.58	-21.39	-1.0905	CCGAUUACGUGAGUAA&UUACUUCACGUAAUCGG	((((((((((((((((&)))).))))))))))))	AUAAUUAUCACUGAACCGUUGUUGUCAUUUGUUUUACAAAAGCAAGGGAUUAAAUCUCCUCCAAUGGACAAAAAAAUGAAUAAUGACAAUACCGAUUACGUGAGUAAUGAAUCAGGGACGCUUUCGCGAUUAUUUAAACUACCUCAGCAU	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0108957	0.9238006	adeQ	948174	adenine permease high affinity adenine:H+ symporter
b1586	-1	24	18	24	4.12	Spot42	5	28	5	11	8.02	-9.23	-21.37	-0.951352	GAUGAAACUCUCAACCUGCUGUGCC&GGUACAGAGGUAAGAUGUUCUAUC	(((..((((((..((((.(((((((&))))))))))).))).)))..)))	UCAUAGUAGCAAAUACAGUACUUUACACGUUAAAUGCUAUGCUUAAAGAAGUUAUCUUCGCGUAAGGAGCUUAUGAUGAAACUCUCAACCUGCUGUGCCGCGCUUCUGCUCGCCCUCGCCUCACCCGCGGUACUCGCCGCGCCGGGAUCC	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0234	0.9238006	ynfD	946133	DUF1161 family periplasmic protein
b3311	17	25	19	25	2.09	Spot42	1	9	1	7	2.1	-9.59	-13.78	-0.988458	GUACUCUGC&GUAGGGUAC	(((((((((&)))))))))	GUCUCACCUGUUGAAGCAAGUGCGUCGCGAUGUCGCACGCGUUAAGACUUUACUGAACGAGAAGGCGGGUGCGUAAUGACCGAUAAAAUCCGUACUCUGCAAGGUCGCGUUGUUAGCGACAAAAUGGAGAAAUCCAUUGUUGUUGCUAUC	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0191007	0.9238006	rpsQ	947808	30S ribosomal subunit protein S17
b1212	15	28	20	26	4.52	Spot42	43	56	45	51	3.25	-13.07	-20.84	-1.34715	CUGGUUACGUGAAG&CUUCACGUAAUCGG	((((((((((((((&))))))))))))))	GCUGGAUAUGCUGAUUGAACCGAUUAUCCAGGAACAUCAGGCCGACCAACUGGCGGCGUUGUCCGAGCAGGAAUAAUGGAAUAUCAACACUGGUUACGUGAAGCAAUAAGCCAACUUCAGGCGAGCGAAAGCCCGCGGCGUGAUGCUGAA	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0026174	0.9238006	prmC	945779	RF-1 and RF-2 N5-glutamine methyltransferase
b3051	19	28	22	28	2.51	Spot42	3	12	3	9	3.46	-10.36	-16.33	-1.06782	UCUGUGCCCU&AGGGUACAGA	((((((((((&))))))))))	UGCAACGCGAUAUUUGGCGGAAAAUAAUCCCUGGCCCCAAAUAUUGUAAUUUAUUAAUCAAAAGGAAAUUUAAUAAUGGAUGAUAUUGUUAAUUCUGUGCCCUCCUGGAUGUUUACCGCAAUUAUUGCCGUAUGCAUUCUGUUUAUUAUU	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0123476	0.9238006	yqiK	947537	PHB family membrane protein function unknown

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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