Input and runtime details for job 6571257 (precomputed example)

Sequence Parameters

	Query ncRNA (short) in FASTA		[.fa]
	Index first query position		1
	Target RNA (long) in FASTA		[.fa]
	Target NCBI RefSeq ID		NC_000913
	All replicons		yes
	Extract sequences around		start codon
	nt upstream (1-300)		75
	nt downstream (1-300)		75
	Index first target position		-75

Output Parameters

	Number of interactions per RNA pair		1
	Suboptimal interaction overlap		can overlap in query
	Max. interaction length		not provided
	Max. absolute energy of an interaction		0
	Max. delta energy above mfe of an interaction		100
	No lonely base pairs		yes
	No GU at helix ends		yes

Seed Parameters

	Min. number of basepairs in seed		7
	Max. Number of mismatches in seed		0
	Maximal energy		0
	Minimal unpaired probability (per RNA)		0
	Seed position (query)		not provided
	Seed position (target)		not provided
	Ignore seeds with GU base pairs		no
	Ignore seeds with GU ends		yes

Folding Parameters

	Temperature for energy computation		37.0
	Access. query: folding window size		150
	Access. query: max. basepair distance		100
	Access. target: folding window size		150
	Access. target: max. basepair distance		100
	Energy parameter set (Vienna package)		Turner model, 2004

Job ID 6571257 (server version trunk)

	Job Submitted & Queued		@ Mon Jan 27 14:18:03 CET 2020
	IntaRNA Started		@ Mon Jan 27 14:20:22 CET 2020
	IntaRNA Finished & Post-Processing		@ Mon Jan 27 14:21:19 CET 2020
	Post-Processing Finished		@ Mon Jan 27 14:22:25 CET 2020
	Job Completed		@ Mon Jan 27 14:22:35 CET 2020

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6571257&toolName=IntaRNA ( 30 days expiry )

Description of the job

Spot42 - E. coli

Output [zip]

Downloadable files

	IntaRNA result table:		[csv]
	Target region plot		[png] [pdf]
	Query region plot		[png] [pdf]
	Functional enrichment file		[txt]
	Functional annotation chart		[html] [pdf] [png]

Target region plot

Query region plot

Function

Identified Interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Show rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Sort by selecting a column name.

Target	Start(T)	End(T)	Start(seed-T)	End(seed-T)	ED(T)	Query	Start(Q)	End(Q)	Start(seed-Q)	End(seed-Q)	ED(Q)	Energy	E(hybrid)	E_norm	RRI subseqs	RRI details	Seq(T)	Seq(Q)	p-value	fdr	gene	geneID	annotation
b4361	-33	-23	-33	-27	0.83	Spot42	55	65	59	65	6.03	-9.75	-16.61	-1.00495	CAGCCAAAUUC&GGAUUUGGCUG	(((((((((((&)))))))))))	CGGACUGCCGAAACGAGAUGUGAAUACGGUCAGCGAACCUGACAGCCAAAUUCCACCAGGAUUCAGAGGGUAACGAUGAAAAACGUUGGCGACCUGAUGCAACGCCUGCAAAAAAUGAUGCCUGCCCAUAUCAAACCCGCAUUCAAAACG	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0174491	0.9238006	dnaC	948864	DNA biosynthesis protein
b1751	-14	-2	-13	-7	3.61	Spot42	21	33	26	32	4.03	-9.65	-17.29	-0.994642	UGAAGGAUAGAAC&GUUCUAUCUUUCA	(((((((((((((&)))))))))))))	CCCGACACAAACAUGUGGAUCUGUCUGCUUCGCGCCGCAGCCCACUUACUCACCCAAAAAAUGAAGGAUAGAACGAUGUUGCAACAUUAUUCAGUGUCAUGGAAAAAAGGACUGGCUGCACUCUGUUUACUGGCUGUUGCUGGGCUUAGC	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0184641	0.9238006	ydjY	946248	putative ferrodoxin-like lipoprotein
b1921	60	75	61	67	0.19	Spot42	49	65	58	64	6.17	-9.65	-16.01	-0.994642	CAGCCAGACCCAUUGC&GUAAUCGGAUUUGGCUG	((((((((((..((((&))))..)).))))))))	CGGUUACGCACUAAACUGGGUAAGUUAUAACGUCAGUAAAUGCCGCACUUUAACUUUGACUACCAGGAGUUCUUAAUGAUGGUGCAGCACCUGAAAAGACGGCCAUUAAGCCGCUAUCUUAAAGACUUUAAACACAGCCAGACCCAUUGC	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0184641	0.9238006	fliZ	946833	RpoS antagonist putative regulator of FliA activity
b3709	-53	-42	-48	-42	3.28	Spot42	1	13	1	7	5.11	-9.62	-18.01	-0.99155	CUCUUACCCUAC&GUAGGGUACAGAG	((((((((((((&)))))))).))))	GGCUAUAAGGAUGUUAGCCACUCUCUUACCCUACAUCCUCAAUAACAAAAAUAGCCUUCCUCUAAAGGUGGCAUCAUGACUGAUCAAGCUGAAAAAAAGCACUCUGCAUUUUGGGGUGUUAUGGUUAUAGCAGGUACAGUAAUUGGUGGA	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0187797	0.9238006	tnaB	948220	tryptophan transporter of low affinity
b3238	-21	-5	-21	-15	2.8	Spot42	39	54	48	54	3.87	-9.61	-16.28	-0.990519	GAUUAUGUCGAGGUAAA&UUUACUUCACGUAAUC	((((((((.((((((((&))))))))))))))))	UUAGUGUAUACUUGAUUUUGUGAUAUGGGUCACGAAACAAAGGCCCAGCUAAAAGAUUAUGUCGAGGUAAAAAUCAUGAAAAUCAAAACCACUGUUGCUGCAUUAAGCGUACUUUCUGUUCUCUCUUUCGGUGCAUUCGCUGCCGACUCC	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0188861	0.9238006	yhcN	947835	cadmium and peroxide resistance protein stress-induced
b0511	-28	10	-28	-22	12.54	Spot42	18	58	52	58	12.19	-9.6	-34.33	-0.989488	AUUCGAUACGUGAAAUUAAGAGGGAUUUAUGGAACAUC&GAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAU	((((((((((((((..(((((((((...((((((((((&))))))))))...))...))))))).))))))).)))))))	AAUAUUUUUAUUUUAUUUGUUCCUCAUAGCUAGAUUAAAACAACGUUAUUCGAUACGUGAAAUUAAGAGGGAUUUAUGGAACAUCAGAGAAAACUAUUCCAGCAACGCGGCUAUAGCGAAGAUCUAUUGCCGAAAACGCAAAGCCAGCGG	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0189931	0.9238006	ybbW	945138	putative allantoin transporter
b3311	17	25	19	25	2.09	Spot42	1	9	1	7	2.1	-9.59	-13.78	-0.988458	GUACUCUGC&GUAGGGUAC	(((((((((&)))))))))	GUCUCACCUGUUGAAGCAAGUGCGUCGCGAUGUCGCACGCGUUAAGACUUUACUGAACGAGAAGGCGGGUGCGUAAUGACCGAUAAAAUCCGUACUCUGCAAGGUCGCGUUGUUAGCGACAAAAUGGAGAAAUCCAUUGUUGUUGCUAUC	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0191007	0.9238006	rpsQ	947808	30S ribosomal subunit protein S17
b3921	-74	-67	-73	-67	0.43	Spot42	50	57	50	56	0.29	-9.55	-10.27	-0.984335	UCCGAUUA&UAAUCGGA	((((((((&))))))))	CUCCGAUUAAAACGAUAUCUGUCGCCCUUGUCAAAAGGGUAGAAUCCUGGAAAAGACAACCAUCAAGGAACUUACAUGACCAUACAGCAAUGGUUAUUCUCAUUUAAAGGGCGUAUUGGACGCCGUGAUUUCUGGAUUUGGAUAGGCCUG	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0195371	0.9238006	yiiR	948417	DUF805 family putative inner membrane protein
b2715	-28	-21	-27	-21	0.47	Spot42	1	8	1	7	2.09	-9.51	-12.07	-0.980212	UACCCUAC&GUAGGGUA	((((((((&))))))))	UUCAGCAUCGGCAUUUUUCCGUCACGUCGACUGAUAACAACUACAUCUACCCUACUGAUAACAGGAUAAAAUCCGAUGGCCAAAAAUUAUGCGGCGCUGGCACGCUCGGUGAUAGCGGCACUGGGCGGCGUUGAUAACAUCUCGGCGGUC	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0199833	0.9238006	ascF	947154	cellobiose/arbutin/salicin-specific PTS enzymes IIB and IC components
b2150	-48	-22	-28	-22	7.03	Spot42	1	24	1	7	7.99	-9.49	-24.51	-0.978151	GAGCAUUUAUCUCAAGCACUACCCUGC&GUAGGGUACAGAGGUAAGAUGUUC	(((((((((((((......((((((((&))))))))..))))).))))))))	ACACUUCAUUAACUCUACUGCCCCGCCGAGCAUUUAUCUCAAGCACUACCCUGCAUAAGAAAAACCGGAGAUACCAUGAAUAAGAAGGUGUUAACCCUGUCUGCUGUGAUGGCCAGCAUGUUAUUCGGUGCCGCUGCACACGCUGCUGAU	GUAGGGUACAGAGGUAAGAUGUUCUAUCUUUCAGACCUUUUACUUCACGUAAUCGGAUUUGGCUGAAUAUUUUAGCCGCCCCAGUCAGUAAUGACUGGGGCGUUUUUUA	0.0202101	0.9238006	mglB	949041	methyl-galactoside transporter subunit

Rows: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 all

Details of Selected Interaction Download Interaction Details

Target

Query

Energy

Hybridization Energy

Unfolding Energy - Target

Unfolding Energy - Query

Position - Target

Position - Query

Position Seed - Target

Position Seed - Query

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When using IntaRNA please cite :

Martin Mann, Patrick R. Wright, and Rolf Backofen
IntaRNA 2.0: enhanced and customizable prediction of RNA–RNA interactions
Nucleic Acids Research, 2017, 45 (W1), W435–W439.
Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Anke Busch, Andreas S. Richter, and Rolf Backofen
IntaRNA: efficient prediction of bacterial sRNA targets incorporating target site accessibility and seed regions
Bioinformatics, 2008, 24 (24), 2849-56.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with IntaRNA version 3.1.3 linking Vienna RNA package 2.4.14

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