Input and runtime details for job 3202356 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3202356 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:02 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:03:47 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:03:55 CET 2018
	Job Completed		@ Sat Feb 17 02:04:28 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3202356&toolName=CopraRNA ( 30 days expiry )

Description of the job

GcvB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
81	0.003764	0.1596	stm0121	ftsL	-18.45	0.031843	91 -- 225	48 -- 186	cell division protein FtsL	_____________90__________________226\n_____________\|___________________\|\n5'-CGU...UGCCG__________A________GAAAA...UGC-3'\n______________GGUGAAAAAG_GGUAG_CU\n______________:\|\|\|\|\|\|\|\|\|_\|\|\|\|\|_\|\|\n______________UCACUUUUUC_CCAUC_GA\n3'-UUU...GUCCU________________A__GCGAU...UCA-5'\n_____________\|___________________\|\n_____________187_________________47\n	133 -- 139	129 -- 135	-20.6582 kcal/mol	4.19087 kcal/mol	6.37024 kcal/mol	90 226@LB@ \| \|@LB@5'-CGU...UGCCG A GAAAA...UGC-3'@LB@ GGUGAAAAAG GGUAG CU@LB@ :\|\|\|\|\|\|\|\|\| \|\|\|\|\| \|\|@LB@ UCACUUUUUC CCAUC GA@LB@3'-UUU...GUCCU A GCGAU...UCA-5'@LB@ \| \|@LB@ 187 47@LB@@LB@@LB@Energy -18.45 kcal/mol@LB@Hybridization Energy -20.6582 kcal/mol@LB@Unfolding Energy - mRNA 4.19087 kcal/mol@LB@Unfolding Energy - sRNA 6.37024 kcal/mol@LB@@LB@Position - mRNA 91 -- 225@LB@Position - sRNA 48 -- 186@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 129 -- 135@LB@
82	0.003783	0.1596	stm0710	ybgH	-19.90	0.018103	103 -- 151	33 -- 90	MFS transporter	_____________102__________________152\n_____________\|____________________\|\n5'-GCG...UUUAA_G__________________GGCUU...UUC-3'\n______________G_AAACACGGCGAUACGGCA\n______________\|_\|\|\|\|\|\|::\|:\|:\|\|::\|\|\n______________C_UUUGUGUUGUUGUGUUGU\n3'-UUU...GUUAA_G__________________AGUGU...UCA-5'\n_____________\|____________________\|\n_____________91___________________32\n	116 -- 122	71 -- 77	-24.8461 kcal/mol	5.94354 kcal/mol	3.80671 kcal/mol	102 152@LB@ \| \|@LB@5'-GCG...UUUAA G GGCUU...UUC-3'@LB@ G AAACACGGCGAUACGGCA@LB@ \| \|\|\|\|\|\|::\|:\|:\|\|::\|\|@LB@ C UUUGUGUUGUUGUGUUGU@LB@3'-UUU...GUUAA G AGUGU...UCA-5'@LB@ \| \|@LB@ 91 32@LB@@LB@@LB@Energy -19.90 kcal/mol@LB@Hybridization Energy -24.8461 kcal/mol@LB@Unfolding Energy - mRNA 5.94354 kcal/mol@LB@Unfolding Energy - sRNA 3.80671 kcal/mol@LB@@LB@Position - mRNA 103 -- 151@LB@Position - sRNA 33 -- 90@LB@Position Seed - mRNA 116 -- 122@LB@Position Seed - sRNA 71 -- 77@LB@
83	0.003855	0.1596	stm1680	ycjI	-14.22	0.156552	151 -- 200	34 -- 86	murein peptide amidase A	_____________150_________201\n_____________\|___________\|\n5'-GAA...CUGAA___________UUCGC...CGG-3'\n______________CAUUAUGGACG\n______________\|\|:\|\|\|\|\|\|\|:\n______________GUGAUACCUGU\n3'-UUU...AAUUA___________CUGUC...UCA-5'\n_____________\|___________\|\n_____________87__________33\n	259 -- 265	159 -- 165	-14.9153 kcal/mol	1.6295 kcal/mol	3.40995 kcal/mol	150 201@LB@ \| \|@LB@5'-GAA...CUGAA UUCGC...CGG-3'@LB@ CAUUAUGGACG@LB@ \|\|:\|\|\|\|\|\|\|:@LB@ GUGAUACCUGU@LB@3'-UUU...AAUUA CUGUC...UCA-5'@LB@ \| \|@LB@ 87 33@LB@@LB@@LB@Energy -14.22 kcal/mol@LB@Hybridization Energy -14.9153 kcal/mol@LB@Unfolding Energy - mRNA 1.6295 kcal/mol@LB@Unfolding Energy - sRNA 3.40995 kcal/mol@LB@@LB@Position - mRNA 151 -- 200@LB@Position - sRNA 34 -- 86@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 159 -- 165@LB@
84	0.003893	0.1596	stm2015	erfK	-27.15	0.001054	109 -- 172	35 -- 90	L D-transpeptidase	_____________108____________________________________________________173\n_____________\|______________________________________________________\|\n5'-GAA...GGAAC____________UAACCCGGGC________________AU_____AA_______AUACU...UGG-3'\n______________GCAAACGCAACA__________GCACAACA_C_CAGCC__GUGAG__AAAAGCU\n______________\|\|\|\|\|\|:\|\|\|\|\|__________:\|\|\|\|\|\|\|_\|_\|\|:\|\|__\|\|:\|\|__\|\|\|\|\|\|:\n______________CGUUUGUGUUGU__________UGUGUUGU_G_GUUGG__CAUUC__UUUUCGG\n3'-UUU...GUUAA______________________________A_U____________GG_______GUAGU...UCA-5'\n_____________\|______________________________________________________\|\n_____________91_____________________________________________________34\n	154 -- 160	49 -- 55	-46.562 kcal/mol	9.44521 kcal/mol	15.2217 kcal/mol	108 173@LB@ \| \|@LB@5'-GAA...GGAAC UAACCCGGGC AU AA AUACU...UGG-3'@LB@ GCAAACGCAACA GCACAACA C CAGCC GUGAG AAAAGCU@LB@ \|\|\|\|\|\|:\|\|\|\|\| :\|\|\|\|\|\|\| \| \|\|:\|\| \|\|:\|\| \|\|\|\|\|\|:@LB@ CGUUUGUGUUGU UGUGUUGU G GUUGG CAUUC UUUUCGG@LB@3'-UUU...GUUAA A U GG GUAGU...UCA-5'@LB@ \| \|@LB@ 91 34@LB@@LB@@LB@Energy -27.15 kcal/mol@LB@Hybridization Energy -46.562 kcal/mol@LB@Unfolding Energy - mRNA 9.44521 kcal/mol@LB@Unfolding Energy - sRNA 15.2217 kcal/mol@LB@@LB@Position - mRNA 109 -- 172@LB@Position - sRNA 35 -- 90@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 49 -- 55@LB@
85	0.003998	0.1596	stm1098	hpaC	-22.15	0.007534	132 -- 187	37 -- 90	4-hydroxyphenylacetate 3-monooxygenase reductase component	_____________131________________________________________188\n_____________\|__________________________________________\|\n5'-UAC...GUUUC___UUU__________G_________AU______GA______GCUGA...GCG-3'\n______________GCA___GCACAAUAAC_ACGACAUCA__CAACUG__UAAGCU\n______________\|\|\|___:\|\|\|\|\|:\|\|\|_\|\|:\|\|\|\|\|\|__\|\|\|\|:\|__\|\|\|\|\|:\n______________CGU___UGUGUUGUUG_UGUUGUAGU__GUUGGC__AUUCGG\n3'-UUU...GUUAA___U______________________________________UUUUC...UCA-5'\n_____________\|__________________________________________\|\n_____________91_________________________________________36\n	151 -- 157	68 -- 74	-38.4357 kcal/mol	7.30976 kcal/mol	11.8287 kcal/mol	131 188@LB@ \| \|@LB@5'-UAC...GUUUC UUU G AU GA GCUGA...GCG-3'@LB@ GCA GCACAAUAAC ACGACAUCA CAACUG UAAGCU@LB@ \|\|\| :\|\|\|\|\|:\|\|\| \|\|:\|\|\|\|\|\| \|\|\|\|:\| \|\|\|\|\|:@LB@ CGU UGUGUUGUUG UGUUGUAGU GUUGGC AUUCGG@LB@3'-UUU...GUUAA U UUUUC...UCA-5'@LB@ \| \|@LB@ 91 36@LB@@LB@@LB@Energy -22.15 kcal/mol@LB@Hybridization Energy -38.4357 kcal/mol@LB@Unfolding Energy - mRNA 7.30976 kcal/mol@LB@Unfolding Energy - sRNA 11.8287 kcal/mol@LB@@LB@Position - mRNA 132 -- 187@LB@Position - sRNA 37 -- 90@LB@Position Seed - mRNA 151 -- 157@LB@Position Seed - sRNA 68 -- 74@LB@
86	0.004005	0.1596	stm1961	fliS	-19.11	0.024619	1 -- 77	23 -- 90	flagellar export chaperone FliS	______________1__________________________________________________________________________________78\n______________\|__________________________________________________________________________________\|\n___________5'-_______G_______UU____C________AA_____UGACAAAACAGUACCU_________C____________________CGUUG...AUG-3'\n______________GC__GCA_GACAACG__AACG_CAC_GCUG__AAGCC________________GUCCGUUAG_AACA_GCAU__CGAUGAAAC\n______________\|\|__:\|\|_:\|\|\|\|\|:__\|\|\|:_\|\|\|_:\|:\|__\|\|\|\|\|________________\|:\|\|:\|:\|\|_\|\|\|\|_\|\|\|\|__\|\|\|\|:\|\|\|\|\n______________CG__UGU_UUGUUGU__UUGU_GUG_UGGC__UUCGG________________CGGGUAGUC_UUGU_CGUA__GCUAUUUUG\n3'-UUU...GUUAA__UU___G_______G_____A___U____A______UUUU__________________________G____AG_________AAAAG...UCA-5'\n_____________\|___________________________________________________________________________________\|\n_____________91__________________________________________________________________________________22\n	7 -- 13	76 -- 82	-54.7378 kcal/mol	16.9215 kcal/mol	25.0607 kcal/mol	1 78@LB@ \| \|@LB@ 5'- G UU C AA UGACAAAACAGUACCU C CGUUG...AUG-3'@LB@ GC GCA GACAACG AACG CAC GCUG AAGCC GUCCGUUAG AACA GCAU CGAUGAAAC@LB@ \|\| :\|\| :\|\|\|\|\|: \|\|\|: \|\|\| :\|:\| \|\|\|\|\| \|:\|\|:\|:\|\| \|\|\|\| \|\|\|\| \|\|\|\|:\|\|\|\|@LB@ CG UGU UUGUUGU UUGU GUG UGGC UUCGG CGGGUAGUC UUGU CGUA GCUAUUUUG@LB@3'-UUU...GUUAA UU G G A U A UUUU G AG AAAAG...UCA-5'@LB@ \| \|@LB@ 91 22@LB@@LB@@LB@Energy -19.11 kcal/mol@LB@Hybridization Energy -54.7378 kcal/mol@LB@Unfolding Energy - mRNA 16.9215 kcal/mol@LB@Unfolding Energy - sRNA 25.0607 kcal/mol@LB@@LB@Position - mRNA 1 -- 77@LB@Position - sRNA 23 -- 90@LB@Position Seed - mRNA 7 -- 13@LB@Position Seed - sRNA 76 -- 82@LB@
87	0.004009	0.1596	stm2067	sbcB	-11.70	0.367652	220 -- 292	35 -- 90	exodeoxyribonuclease I	_____________219_____________________293\n_____________\|_______________________\|\n5'-AUU...AUGUA______U________________AUUGG...CCG-3'\n______________CGAACA_UAGCA__ACAAUAUCA\n______________\|:\|\|\|\|_:\|:\|\|__\|\|\|\|:\|\|\|\|\n______________GUUUGU_GUUGU__UGUUGUAGU\n3'-UUU...UUAAC____________UG_________GUUGG...UCA-5'\n_____________\|_______________________\|\n_____________91______________________34\n	110 -- 116	68 -- 74	-17.8808 kcal/mol	2.08887 kcal/mol	5.27986 kcal/mol	219 293@LB@ \| \|@LB@5'-AUU...AUGUA U AUUGG...CCG-3'@LB@ CGAACA UAGCA ACAAUAUCA@LB@ \|:\|\|\|\| :\|:\|\| \|\|\|\|:\|\|\|\|@LB@ GUUUGU GUUGU UGUUGUAGU@LB@3'-UUU...UUAAC UG GUUGG...UCA-5'@LB@ \| \|@LB@ 91 34@LB@@LB@@LB@Energy -11.70 kcal/mol@LB@Hybridization Energy -17.8808 kcal/mol@LB@Unfolding Energy - mRNA 2.08887 kcal/mol@LB@Unfolding Energy - sRNA 5.27986 kcal/mol@LB@@LB@Position - mRNA 220 -- 292@LB@Position - sRNA 35 -- 90@LB@Position Seed - mRNA 110 -- 116@LB@Position Seed - sRNA 68 -- 74@LB@
88	0.00402	0.1596	stm2651	yfiQ	-16.02	0.080775	116 -- 159	50 -- 88	protein acetyltransferase	_____________115__________________________160\n_____________\|____________________________\|\n5'-CAA...AGGAA__________UCCGGGA___________AUAAA...GUA-3'\n______________AAACGCAACA_______AACGUCACAGC\n______________\|\|\|\|:\|\|\|\|\|_______\|\|\|:\|\|\|\|\|:\|\n______________UUUGUGUUGU_______UUGUAGUGUUG\n3'-UUU...UAACG__________UGUG______________GCAUU...UCA-5'\n_____________\|____________________________\|\n_____________89___________________________49\n	137 -- 143	64 -- 70	-25.6269 kcal/mol	4.45838 kcal/mol	7.90699 kcal/mol	115 160@LB@ \| \|@LB@5'-CAA...AGGAA UCCGGGA AUAAA...GUA-3'@LB@ AAACGCAACA AACGUCACAGC@LB@ \|\|\|\|:\|\|\|\|\| \|\|\|:\|\|\|\|\|:\|@LB@ UUUGUGUUGU UUGUAGUGUUG@LB@3'-UUU...UAACG UGUG GCAUU...UCA-5'@LB@ \| \|@LB@ 89 49@LB@@LB@@LB@Energy -16.02 kcal/mol@LB@Hybridization Energy -25.6269 kcal/mol@LB@Unfolding Energy - mRNA 4.45838 kcal/mol@LB@Unfolding Energy - sRNA 7.90699 kcal/mol@LB@@LB@Position - mRNA 116 -- 159@LB@Position - sRNA 50 -- 88@LB@Position Seed - mRNA 137 -- 143@LB@Position Seed - sRNA 64 -- 70@LB@
89	0.004143	0.1596	stm2957	ygcA	-15.40	0.101796	159 -- 208	35 -- 82	23S rRNA (uracil(1939)-C(5))-methyltransferase	_____________158____________________________________________209\n_____________\|______________________________________________\|\n5'-AUA...UACUC_____AACG____GU_________UCAG___________UA_____AAUGA...GUC-3'\n______________UGCAA____GCGC__GACGACGCG____AUCAUAACCGU__AAGUC\n______________\|\|\|\|\|____:\|:\|__:\|\|:\|\|:\|:____\|\|\|\|:\|\|\|\|\|\|__\|\|\|:\|\n______________ACGUU____UGUG__UUGUUGUGU____UAGUGUUGGCA__UUCGG\n3'-UUU...GGUUA________________________UG____________________UUUUC...UCA-5'\n_____________\|______________________________________________\|\n_____________83_____________________________________________34\n	250 -- 256	61 -- 67	-36.475 kcal/mol	14.3441 kcal/mol	11.8308 kcal/mol	158 209@LB@ \| \|@LB@5'-AUA...UACUC AACG GU UCAG UA AAUGA...GUC-3'@LB@ UGCAA GCGC GACGACGCG AUCAUAACCGU AAGUC@LB@ \|\|\|\|\| :\|:\| :\|\|:\|\|:\|: \|\|\|\|:\|\|\|\|\|\| \|\|\|:\|@LB@ ACGUU UGUG UUGUUGUGU UAGUGUUGGCA UUCGG@LB@3'-UUU...GGUUA UG UUUUC...UCA-5'@LB@ \| \|@LB@ 83 34@LB@@LB@@LB@Energy -15.40 kcal/mol@LB@Hybridization Energy -36.475 kcal/mol@LB@Unfolding Energy - mRNA 14.3441 kcal/mol@LB@Unfolding Energy - sRNA 11.8308 kcal/mol@LB@@LB@Position - mRNA 159 -- 208@LB@Position - sRNA 35 -- 82@LB@Position Seed - mRNA 250 -- 256@LB@Position Seed - sRNA 61 -- 67@LB@
90	0.004161	0.1596	stm1124	putA	-17.89	0.039481	159 -- 201	125 -- 168	trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase	_____________158______________________________________________202\n_____________\|________________________________________________\|\n5'-UAA...CGCUA____U___UUA________A_C____A_____________________UGGGA...UAA-3'\n______________ACUA_UGG___GCAGAUAG_G_GAAU__ACAGG_AGU___AGAGGGUA\n______________\|\|\|\|_\|\|\|___:\|\|\|\|:\|\|_\|_:\|\|\|__\|\|\|\|\|_\|\|\|___\|:\|\|\|\|\|\|\n______________UGAU_ACC___UGUCUGUC_C_UUUA__UGUCC_UCA___UUUCCCAU\n3'-UUU...AUUAG___________________C_A____CA_____U___CUU________CAGAG...UCA-5'\n_____________\|________________________________________________\|\n_____________169______________________________________________124\n	195 -- 201	125 -- 131	-33.9858 kcal/mol	7.26317 kcal/mol	8.4265 kcal/mol	158 202@LB@ \| \|@LB@5'-UAA...CGCUA U UUA A C A UGGGA...UAA-3'@LB@ ACUA UGG GCAGAUAG G GAAU ACAGG AGU AGAGGGUA@LB@ \|\|\|\| \|\|\| :\|\|\|\|:\|\| \| :\|\|\| \|\|\|\|\| \|\|\| \|:\|\|\|\|\|\|@LB@ UGAU ACC UGUCUGUC C UUUA UGUCC UCA UUUCCCAU@LB@3'-UUU...AUUAG C A CA U CUU CAGAG...UCA-5'@LB@ \| \|@LB@ 169 124@LB@@LB@@LB@Energy -17.89 kcal/mol@LB@Hybridization Energy -33.9858 kcal/mol@LB@Unfolding Energy - mRNA 7.26317 kcal/mol@LB@Unfolding Energy - sRNA 8.4265 kcal/mol@LB@@LB@Position - mRNA 159 -- 201@LB@Position - sRNA 125 -- 168@LB@Position Seed - mRNA 195 -- 201@LB@Position Seed - sRNA 125 -- 131@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching