Input and runtime details for job 3202356 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3202356 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:02 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:03:47 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:03:55 CET 2018
	Job Completed		@ Sat Feb 17 02:04:28 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3202356&toolName=CopraRNA ( 30 days expiry )

Description of the job

GcvB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
69	0.003278	0.1596	stm3675	sgbH	-16.55	0.065932	154 -- 200	33 -- 93	3-keto-L-gulonate-6-phosphate decarboxylase	_____________153_______________________201\n_____________\|_________________________\|\n5'-UUC...CAGUC__G_____GCC______________CCUGC...AGG-3'\n______________UG_AAGCA___CAACGCGAUGUCGC\n______________\|\|_\|\|:\|\|___\|\|\|\|:\|:\|::\|\|:\|\n______________AC_UUUGU___GUUGUGUUGUAGUG\n3'-UUU...GGUUA__G_____GUU______________UUGGC...UCA-5'\n_____________\|_________________________\|\n_____________94________________________32\n	258 -- 264	71 -- 77	-24.2274 kcal/mol	7.7572 kcal/mol	5.6854 kcal/mol	153 201@LB@ \| \|@LB@5'-UUC...CAGUC G GCC CCUGC...AGG-3'@LB@ UG AAGCA CAACGCGAUGUCGC@LB@ \|\| \|\|:\|\| \|\|\|\|:\|:\|::\|\|:\|@LB@ AC UUUGU GUUGUGUUGUAGUG@LB@3'-UUU...GGUUA G GUU UUGGC...UCA-5'@LB@ \| \|@LB@ 94 32@LB@@LB@@LB@Energy -16.55 kcal/mol@LB@Hybridization Energy -24.2274 kcal/mol@LB@Unfolding Energy - mRNA 7.7572 kcal/mol@LB@Unfolding Energy - sRNA 5.6854 kcal/mol@LB@@LB@Position - mRNA 154 -- 200@LB@Position - sRNA 33 -- 93@LB@Position Seed - mRNA 258 -- 264@LB@Position Seed - sRNA 71 -- 77@LB@
36	0.00125	0.1215	stm3523	glpR	-16.55	0.065942	207 -- 266	37 -- 89	DeoR/GlpR family transcriptional regulator	_____________206________________267\n_____________\|__________________\|\n5'-UUG...UGAAA__________________GCGAU...CGC-3'\n______________CAAACACAACGGCAUGAC\n______________\|\|\|\|\|\|\|\|\|\|::\|\|::\|\|\n______________GUUUGUGUUGUUGUGUUG\n3'-UUU...UUAAC__________________UAGUG...UCA-5'\n_____________\|__________________\|\n_____________90_________________36\n	218 -- 224	72 -- 78	-24.6143 kcal/mol	5.8046 kcal/mol	3.79561 kcal/mol	206 267@LB@ \| \|@LB@5'-UUG...UGAAA GCGAU...CGC-3'@LB@ CAAACACAACGGCAUGAC@LB@ \|\|\|\|\|\|\|\|\|\|::\|\|::\|\|@LB@ GUUUGUGUUGUUGUGUUG@LB@3'-UUU...UUAAC UAGUG...UCA-5'@LB@ \| \|@LB@ 90 36@LB@@LB@@LB@Energy -16.55 kcal/mol@LB@Hybridization Energy -24.6143 kcal/mol@LB@Unfolding Energy - mRNA 5.8046 kcal/mol@LB@Unfolding Energy - sRNA 3.79561 kcal/mol@LB@@LB@Position - mRNA 207 -- 266@LB@Position - sRNA 37 -- 89@LB@Position Seed - mRNA 218 -- 224@LB@Position Seed - sRNA 72 -- 78@LB@
14	0.0002233	0.05681	stm3843	trmE	-16.53	0.066478	207 -- 249	37 -- 85	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	_____________206______________250\n_____________\|________________\|\n5'-UGA...CAUGA________________UAUCG...CGC-3'\n______________GC___CAUAACGACAC\n______________\|\|___\|\|:\|\|\|:\|\|\|\|\n______________CG___GUGUUGUUGUG\n3'-UUU...GUUAA__UUU___________UUGUA...UCA-5'\n_____________\|________________\|\n_____________86_______________36\n	211 -- 217	75 -- 81	-15.3625 kcal/mol	2.22645 kcal/mol	3.72363 kcal/mol	206 250@LB@ \| \|@LB@5'-UGA...CAUGA UAUCG...CGC-3'@LB@ GC CAUAACGACAC@LB@ \|\| \|\|:\|\|\|:\|\|\|\|@LB@ CG GUGUUGUUGUG@LB@3'-UUU...GUUAA UUU UUGUA...UCA-5'@LB@ \| \|@LB@ 86 36@LB@@LB@@LB@Energy -16.53 kcal/mol@LB@Hybridization Energy -15.3625 kcal/mol@LB@Unfolding Energy - mRNA 2.22645 kcal/mol@LB@Unfolding Energy - sRNA 3.72363 kcal/mol@LB@@LB@Position - mRNA 207 -- 249@LB@Position - sRNA 37 -- 85@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 75 -- 81@LB@
59	0.00232	0.14	stm4105	metF	-16.51	0.066936	223 -- 245	40 -- 59	methylenetetrahydrofolate reductase [NAD(P)H]	_____________222__________________246\n_____________\|____________________\|\n5'-AAG...UUUAC__________AUA_______GCGAU...GCA-3'\n______________AACACAACAA___AUAACAU\n______________\|\|\|\|\|\|\|\|\|\|___\|:\|\|\|\|\|\n______________UUGUGUUGUU___UGUUGUA\n3'-UUU...AACGU__________G_________GUGUU...UCA-5'\n_____________\|____________________\|\n_____________60___________________39\n	175 -- 181	70 -- 76	-16.6304 kcal/mol	0.675865 kcal/mol	3.87174 kcal/mol	222 246@LB@ \| \|@LB@5'-AAG...UUUAC AUA GCGAU...GCA-3'@LB@ AACACAACAA AUAACAU@LB@ \|\|\|\|\|\|\|\|\|\| \|:\|\|\|\|\|@LB@ UUGUGUUGUU UGUUGUA@LB@3'-UUU...AACGU G GUGUU...UCA-5'@LB@ \| \|@LB@ 60 39@LB@@LB@@LB@Energy -16.51 kcal/mol@LB@Hybridization Energy -16.6304 kcal/mol@LB@Unfolding Energy - mRNA 0.675865 kcal/mol@LB@Unfolding Energy - sRNA 3.87174 kcal/mol@LB@@LB@Position - mRNA 223 -- 245@LB@Position - sRNA 40 -- 59@LB@Position Seed - mRNA 175 -- 181@LB@Position Seed - sRNA 70 -- 76@LB@
63	0.003024	0.1596	stm3563	livH	-16.49	0.067433	27 -- 175	36 -- 184	branched-chain amino acid ABC transporter permease LivH	_____________26_____________176\n_____________\|______________\|\n5'-GAU...GAAAU______________CGAUC...ACG-3'\n______________GGGA_UGAAAAAGG\n______________\|\|:\|_\|\|\|\|\|\|\|\|\|\n______________CCUU_ACUUUUUCC\n3'-UUU...CAUGU____C_________CAUCA...UCA-5'\n_____________\|______________\|\n_____________185____________35\n	65 -- 71	128 -- 134	-14.2251 kcal/mol	3.55711 kcal/mol	3.77758 kcal/mol	26 176@LB@ \| \|@LB@5'-GAU...GAAAU CGAUC...ACG-3'@LB@ GGGA UGAAAAAGG@LB@ \|\|:\| \|\|\|\|\|\|\|\|\|@LB@ CCUU ACUUUUUCC@LB@3'-UUU...CAUGU C CAUCA...UCA-5'@LB@ \| \|@LB@ 185 35@LB@@LB@@LB@Energy -16.49 kcal/mol@LB@Hybridization Energy -14.2251 kcal/mol@LB@Unfolding Energy - mRNA 3.55711 kcal/mol@LB@Unfolding Energy - sRNA 3.77758 kcal/mol@LB@@LB@Position - mRNA 27 -- 175@LB@Position - sRNA 36 -- 184@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 128 -- 134@LB@
53	0.002045	0.1363	stm1876	holE	-16.46	0.068295	21 -- 75	31 -- 90	DNA polymerase III subunit theta	_____________20__________________________________________76\n_____________\|___________________________________________\|\n5'-CGU...UGCCA_______GUAA___UA___________G___C__A________AUAUU...UGC-3'\n______________GC_AGCA____AGC__AACGUCGCGAU_GUG_AG__AGAAGCC\n______________\|\|_\|:\|\|____\|:\|__\|\|\|:\|\|:\|:\|:_\|\|:_\|\|__\|:\|\|\|\|\|\n______________CG_UUGU____UUG__UUGUAGUGUUG_CAU_UC__UUUUCGG\n3'-UUU...GUUAA__U____GUUG___UG___________G______GG_______GUAGU...UCA-5'\n_____________\|___________________________________________\|\n_____________91__________________________________________30\n	55 -- 61	49 -- 55	-35.8289 kcal/mol	10.2843 kcal/mol	15.2217 kcal/mol	20 76@LB@ \| \|@LB@5'-CGU...UGCCA GUAA UA G C A AUAUU...UGC-3'@LB@ GC AGCA AGC AACGUCGCGAU GUG AG AGAAGCC@LB@ \|\| \|:\|\| \|:\| \|\|\|:\|\|:\|:\|: \|\|: \|\| \|:\|\|\|\|\|@LB@ CG UUGU UUG UUGUAGUGUUG CAU UC UUUUCGG@LB@3'-UUU...GUUAA U GUUG UG G GG GUAGU...UCA-5'@LB@ \| \|@LB@ 91 30@LB@@LB@@LB@Energy -16.46 kcal/mol@LB@Hybridization Energy -35.8289 kcal/mol@LB@Unfolding Energy - mRNA 10.2843 kcal/mol@LB@Unfolding Energy - sRNA 15.2217 kcal/mol@LB@@LB@Position - mRNA 21 -- 75@LB@Position - sRNA 31 -- 90@LB@Position Seed - mRNA 55 -- 61@LB@Position Seed - sRNA 49 -- 55@LB@
29	0.0007392	0.08814	stm3096	yqgE	-16.45	0.068552	198 -- 251	37 -- 84	DUF179 domain-containing protein	_____________197___________252\n_____________\|_____________\|\n5'-UGG...UAUAU_____________CAGGA...GCG-3'\n______________UUGCGAGCACAAC\n______________\|\|\|\|:\|:\|\|\|\|\|\|\n______________AACGUUUGUGUUG\n3'-UUU...UGGUU_____________UUGUG...UCA-5'\n_____________\|_____________\|\n_____________85____________36\n	284 -- 290	80 -- 86	-18.5161 kcal/mol	2.82597 kcal/mol	3.5889 kcal/mol	197 252@LB@ \| \|@LB@5'-UGG...UAUAU CAGGA...GCG-3'@LB@ UUGCGAGCACAAC@LB@ \|\|\|\|:\|:\|\|\|\|\|\|@LB@ AACGUUUGUGUUG@LB@3'-UUU...UGGUU UUGUG...UCA-5'@LB@ \| \|@LB@ 85 36@LB@@LB@@LB@Energy -16.45 kcal/mol@LB@Hybridization Energy -18.5161 kcal/mol@LB@Unfolding Energy - mRNA 2.82597 kcal/mol@LB@Unfolding Energy - sRNA 3.5889 kcal/mol@LB@@LB@Position - mRNA 198 -- 251@LB@Position - sRNA 37 -- 84@LB@Position Seed - mRNA 284 -- 290@LB@Position Seed - sRNA 80 -- 86@LB@
108	0.005311	0.1728	stm2183	cdd	-16.35	0.071230	31 -- 68	151 -- 187	cytidine deaminase	_____________30_________________69\n_____________\|__________________\|\n5'-UGG...AGAUC_____UUAA_________GGCCC...UGA-3'\n______________ACAUA____GACAUAGCA\n______________\|\|\|:\|____:\|\|\|:\|:\|\|\n______________UGUGU____UUGUGUUGU\n3'-UUU...ACGUU_____UG___________AGUGU...UCA-5'\n_____________\|__________________\|\n_____________188________________150\n	150 -- 156	71 -- 77	-12.389 kcal/mol	1.12571 kcal/mol	3.50787 kcal/mol	30 69@LB@ \| \|@LB@5'-UGG...AGAUC UUAA GGCCC...UGA-3'@LB@ ACAUA GACAUAGCA@LB@ \|\|\|:\| :\|\|\|:\|:\|\|@LB@ UGUGU UUGUGUUGU@LB@3'-UUU...ACGUU UG AGUGU...UCA-5'@LB@ \| \|@LB@ 188 150@LB@@LB@@LB@Energy -16.35 kcal/mol@LB@Hybridization Energy -12.389 kcal/mol@LB@Unfolding Energy - mRNA 1.12571 kcal/mol@LB@Unfolding Energy - sRNA 3.50787 kcal/mol@LB@@LB@Position - mRNA 31 -- 68@LB@Position - sRNA 151 -- 187@LB@Position Seed - mRNA 150 -- 156@LB@Position Seed - sRNA 71 -- 77@LB@
18	0.0003131	0.06194	stm0112	leuB	-16.29	0.072835	153 -- 217	44 -- 93	3-isopropylmalate dehydrogenase	_____________152___________218\n_____________\|_____________\|\n5'-AAA...AAAGA_____________AGCUC...GUU-3'\n______________AUUGC_AACGCAA\n______________\|\|\|\|\|_\|\|\|:\|\|\|\n______________UAACG_UUGUGUU\n3'-UUU...CUGGU_____U_______GUUGU...UCA-5'\n_____________\|_____________\|\n_____________94____________43\n	158 -- 164	81 -- 87	-11.6192 kcal/mol	1.90811 kcal/mol	3.57619 kcal/mol	152 218@LB@ \| \|@LB@5'-AAA...AAAGA AGCUC...GUU-3'@LB@ AUUGC AACGCAA@LB@ \|\|\|\|\| \|\|\|:\|\|\|@LB@ UAACG UUGUGUU@LB@3'-UUU...CUGGU U GUUGU...UCA-5'@LB@ \| \|@LB@ 94 43@LB@@LB@@LB@Energy -16.29 kcal/mol@LB@Hybridization Energy -11.6192 kcal/mol@LB@Unfolding Energy - mRNA 1.90811 kcal/mol@LB@Unfolding Energy - sRNA 3.57619 kcal/mol@LB@@LB@Position - mRNA 153 -- 217@LB@Position - sRNA 44 -- 93@LB@Position Seed - mRNA 158 -- 164@LB@Position Seed - sRNA 81 -- 87@LB@
91	0.004202	0.1596	stm0387	yaiI	-16.26	0.073758	5 -- 45	23 -- 58	similar to E. coli orf hypothetical protein (AAC73490.1); Blastp hit to AAC73490.1 (192 aa) 89% identity in aa 41 - 191	_____________4____________________________________________46\n_____________\|____________________________________________\|\n_______5'-GUCC_______AUAGCCUU______AUU________G___A_______CGAUU...CCA-3'\n______________GCCAAGA________GCCCAU____AC_CGCA__CC_AUAAAAC\n______________\|\|\|\|\|:\|________\|\|\|\|\|\|____\|\|_\|\|\|\|__\|\|_\|\|\|\|\|\|\|\n______________CGGUUUU________CGGGUA____UG_GCGU__GG_UAUUUUG\n3'-UUU...GCAUU_____________________GUCU__U____AA__C_______AAAAG...UCA-5'\n_____________\|____________________________________________\|\n_____________59___________________________________________22\n	5 -- 11	52 -- 58	-32.2087 kcal/mol	3.21436 kcal/mol	17.1718 kcal/mol	4 46@LB@ \| \|@LB@ 5'-GUCC AUAGCCUU AUU G A CGAUU...CCA-3'@LB@ GCCAAGA GCCCAU AC CGCA CC AUAAAAC@LB@ \|\|\|\|\|:\| \|\|\|\|\|\| \|\| \|\|\|\| \|\| \|\|\|\|\|\|\|@LB@ CGGUUUU CGGGUA UG GCGU GG UAUUUUG@LB@3'-UUU...GCAUU GUCU U AA C AAAAG...UCA-5'@LB@ \| \|@LB@ 59 22@LB@@LB@@LB@Energy -16.26 kcal/mol@LB@Hybridization Energy -32.2087 kcal/mol@LB@Unfolding Energy - mRNA 3.21436 kcal/mol@LB@Unfolding Energy - sRNA 17.1718 kcal/mol@LB@@LB@Position - mRNA 5 -- 45@LB@Position - sRNA 23 -- 58@LB@Position Seed - mRNA 5 -- 11@LB@Position Seed - sRNA 52 -- 58@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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