| 81 |
0.002798 |
0.1325 |
bsu03250 |
ycgR |
-7.37 |
0.727097 |
84 -- 130 |
48 -- 86 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pt: transporter' |
|
_____________83_______________________________________________131\n_____________|________________________________________________|\n5'-GCG...UACUC__________G____CUAACCAAAGUG________CUUUC___UC___CGUCA...GAA-3'\n______________GGCUUU_UUG_AAUC____________AAACGAUC_____CCC__CUC\n______________|||||:_|||_||||____________||||:|||_____|||__|||\n______________CCGAAG_AAC_UUAG____________UUUGUUAG_____GGG__GAG\n3'-AUU...ACUUA______C___G____AACCG_______________CAU_____UA___AAUAA...CAA-5'\n_____________|________________________________________________|\n_____________87_______________________________________________47\n |
111 -- 117 |
59 -- 65 |
-26.5811 kcal/mol |
9.91993 kcal/mol |
9.18845 kcal/mol |
83 131@LB@ | |@LB@5'-GCG...UACUC G CUAACCAAAGUG CUUUC UC CGUCA...GAA-3'@LB@ GGCUUU UUG AAUC AAACGAUC CCC CUC@LB@ |||||: ||| |||| ||||:||| ||| |||@LB@ CCGAAG AAC UUAG UUUGUUAG GGG GAG@LB@3'-AUU...ACUUA C G AACCG CAU UA AAUAA...CAA-5'@LB@ | |@LB@ 87 47@LB@@LB@@LB@Energy -7.37 kcal/mol@LB@Hybridization Energy -26.5811 kcal/mol@LB@Unfolding Energy - mRNA 9.91993 kcal/mol@LB@Unfolding Energy - sRNA 9.18845 kcal/mol@LB@@LB@Position - mRNA 84 -- 130@LB@Position - sRNA 48 -- 86@LB@Position Seed - mRNA 111 -- 117@LB@Position Seed - sRNA 59 -- 65@LB@ |
| 82 |
0.002803 |
0.1325 |
bsu34100 |
rsbQ |
-17.18 |
0.011511 |
5 -- 86 |
34 -- 116 |
sigma factor SigB regulation protein RsbQ |
|
_____________4__________________________________________________________________________________________87\n_____________|__________________________________________________________________________________________|\n_______5'-CAAA____________C__ACCCC_____________G__UAG______CUCCUCCU___UCUUC_____C____________CUA__U_____GUCCU...UGU-3'\n______________UGUCAUUUUUUU_UC_______________GGU_UC___GCAAUC________GCA_____CAAUU__AUCCAUCUUUU___UC_CCCAU\n______________|||||:|||:|:_||_______________||:_||___||||||________|||_____||||:__|:||||||:||___||_|||||\n______________ACAGUGAAAGAG_AG_______________CCG_AG___CGUUAG________CGU_____GUUAG__UGGGUAGAGAA___AG_GGGUA\n3'-AUU...UUACA____________U__AAGUUUCAUUACUUA___A__CAA______AAC________UU________CA___________UA_________AAGAA...CAA-5'\n_____________|__________________________________________________________________________________________|\n_____________117________________________________________________________________________________________33\n |
65 -- 71 |
47 -- 53 |
-43.7075 kcal/mol |
8.64393 kcal/mol |
22.4324 kcal/mol |
4 87@LB@ | |@LB@ 5'-CAAA C ACCCC G UAG CUCCUCCU UCUUC C CUA U GUCCU...UGU-3'@LB@ UGUCAUUUUUUU UC GGU UC GCAAUC GCA CAAUU AUCCAUCUUUU UC CCCAU@LB@ |||||:|||:|: || ||: || |||||| ||| ||||: |:||||||:|| || |||||@LB@ ACAGUGAAAGAG AG CCG AG CGUUAG CGU GUUAG UGGGUAGAGAA AG GGGUA@LB@3'-AUU...UUACA U AAGUUUCAUUACUUA A CAA AAC UU CA UA AAGAA...CAA-5'@LB@ | |@LB@ 117 33@LB@@LB@@LB@Energy -17.18 kcal/mol@LB@Hybridization Energy -43.7075 kcal/mol@LB@Unfolding Energy - mRNA 8.64393 kcal/mol@LB@Unfolding Energy - sRNA 22.4324 kcal/mol@LB@@LB@Position - mRNA 5 -- 86@LB@Position - sRNA 34 -- 116@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 47 -- 53@LB@ |
| 83 |
0.002931 |
0.1368 |
bsu18650 |
pelB |
-17.38 |
0.010445 |
17 -- 103 |
38 -- 142 |
pectin lyase |
|
_____________16________________________________________________________________________________________________________________104\n_____________|_________________________________________________________________________________________________________________|\n5'-AGG...UAAGC_________U_______G_____UCAA____________CUA_________________________A___U____UU__________________________AGUAA____ACAUU...CAA-3'\n______________UG_CUGCAG_CAA___C_GUGUA______UC_CUUUCUU___UUUCAA______________UUGUU_GUA_GUCU__UGGC__GCAAUC___UCUAUUUUUUA_____CCCA\n______________||_|:||||_|||___|_:|:||______||_||||||:___:|||||______________|:|||_|:|_:|||__||||__:|||||___:|:||:|:|||_____||||\n______________AC_GGCGUC_GUU___G_UAUAU______AG_GAAAGAG___GAAGUU______________AGCAA_CGU_UAGA__ACCG__UGUUAG___GGGUAGAGAAU_____GGGU\n3'-AUU...CUGUA__A__________CAA_G_____UACAAC__U_______UA_______UCAUUACUUACCGA____________________UU______CAU___________AAG______AAAGA...CAA-5'\n_____________|_________________________________________________________________________________________________________________|\n_____________143_______________________________________________________________________________________________________________37\n |
42 -- 48 |
105 -- 111 |
-52.303 kcal/mol |
11.0023 kcal/mol |
27.6117 kcal/mol |
16 104@LB@ | |@LB@5'-AGG...UAAGC U G UCAA CUA A U UU AGUAA ACAUU...CAA-3'@LB@ UG CUGCAG CAA C GUGUA UC CUUUCUU UUUCAA UUGUU GUA GUCU UGGC GCAAUC UCUAUUUUUUA CCCA@LB@ || |:|||| ||| | :|:|| || ||||||: :||||| |:||| |:| :||| |||| :||||| :|:||:|:||| ||||@LB@ AC GGCGUC GUU G UAUAU AG GAAAGAG GAAGUU AGCAA CGU UAGA ACCG UGUUAG GGGUAGAGAAU GGGU@LB@3'-AUU...CUGUA A CAA G UACAAC U UA UCAUUACUUACCGA UU CAU AAG AAAGA...CAA-5'@LB@ | |@LB@ 143 37@LB@@LB@@LB@Energy -17.38 kcal/mol@LB@Hybridization Energy -52.303 kcal/mol@LB@Unfolding Energy - mRNA 11.0023 kcal/mol@LB@Unfolding Energy - sRNA 27.6117 kcal/mol@LB@@LB@Position - mRNA 17 -- 103@LB@Position - sRNA 38 -- 142@LB@Position Seed - mRNA 42 -- 48@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 84 |
0.003073 |
0.1418 |
bsu31450 |
kinB |
-10.05 |
0.307083 |
220 -- 261 |
31 -- 74 |
sporulation kinase B |
|
_____________219___________________262\n_____________|_____________________|\n5'-AUG...CUUUA______________CU_____ACCAA...UGC-3'\n______________UUUUGUUUCCUAUU__UCUUU\n______________|:||:||:||:|||__|||||\n______________AGAAUAAGGGGUAA__AGAAA\n3'-AUU...GGUAG_____________________CUUUA...CAA-5'\n_____________|_____________________|\n_____________75____________________30\n |
248 -- 254 |
36 -- 42 |
-15.4229 kcal/mol |
1.85906 kcal/mol |
5.65941 kcal/mol |
219 262@LB@ | |@LB@5'-AUG...CUUUA CU ACCAA...UGC-3'@LB@ UUUUGUUUCCUAUU UCUUU@LB@ |:||:||:||:||| |||||@LB@ AGAAUAAGGGGUAA AGAAA@LB@3'-AUU...GGUAG CUUUA...CAA-5'@LB@ | |@LB@ 75 30@LB@@LB@@LB@Energy -10.05 kcal/mol@LB@Hybridization Energy -15.4229 kcal/mol@LB@Unfolding Energy - mRNA 1.85906 kcal/mol@LB@Unfolding Energy - sRNA 5.65941 kcal/mol@LB@@LB@Position - mRNA 220 -- 261@LB@Position - sRNA 31 -- 74@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 36 -- 42@LB@ |
| 85 |
0.003124 |
0.1423 |
bsu15550 |
pyrF |
-13.42 |
0.070218 |
194 -- 221 |
50 -- 78 |
orotidine 5'-phosphate decarboxylase |
|
_____________193___________________________222\n_____________|_____________________________|\n5'-CCC...GGAGC__G____A__AA________C________AUCGC...AGG-3'\n______________UG_AAUC_UG__AAACAA_C_UGCCCAUC\n______________||_||||_||__||||||_|_|:||||||\n______________AC_UUAG_AC__UUUGUU_G_AUGGGUAG\n3'-AUU...AAGCA__G____A__CG______A_C________AGAAU...CAA-5'\n_____________|_____________________________|\n_____________79____________________________49\n |
215 -- 221 |
50 -- 56 |
-24.8332 kcal/mol |
3.07511 kcal/mol |
8.45904 kcal/mol |
193 222@LB@ | |@LB@5'-CCC...GGAGC G A AA C AUCGC...AGG-3'@LB@ UG AAUC UG AAACAA C UGCCCAUC@LB@ || |||| || |||||| | |:||||||@LB@ AC UUAG AC UUUGUU G AUGGGUAG@LB@3'-AUU...AAGCA G A CG A C AGAAU...CAA-5'@LB@ | |@LB@ 79 49@LB@@LB@@LB@Energy -13.42 kcal/mol@LB@Hybridization Energy -24.8332 kcal/mol@LB@Unfolding Energy - mRNA 3.07511 kcal/mol@LB@Unfolding Energy - sRNA 8.45904 kcal/mol@LB@@LB@Position - mRNA 194 -- 221@LB@Position - sRNA 50 -- 78@LB@Position Seed - mRNA 215 -- 221@LB@Position Seed - sRNA 50 -- 56@LB@ |
| 86 |
0.003162 |
0.1423 |
bsu32080 |
yuiB |
-17.14 |
0.011766 |
214 -- 271 |
6 -- 63 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________213____________________________________________________________272\n_____________|______________________________________________________________|\n5'-UAG...UCUGC________A_______AA_______G_U____GUC___UUU_______U_____________AUUAA...GGA-3'\n______________CAGUCGUA_UCAUCUC__UUGUUUU_U_UUUU___UUG___UUUGGAA_CG____GUUUCUU\n______________||:|||||_:||||||__||:||::_:_|||:___|||___||||:||_||____:||||||\n______________GUUAGCAU_GGUAGAG__AAUAAGG_G_AAAG___AAC___AAACUUU_GC____UAAAGAA\n3'-AUU...CGUUU________G________________G_U____A_____UUU_______U__GAAU_______AACAA-5'\n_____________|______________________________________________________________|\n_____________64_____________________________________________________________5\n |
265 -- 271 |
6 -- 12 |
-35.4392 kcal/mol |
4.76592 kcal/mol |
13.9327 kcal/mol |
213 272@LB@ | |@LB@5'-UAG...UCUGC A AA G U GUC UUU U AUUAA...GGA-3'@LB@ CAGUCGUA UCAUCUC UUGUUUU U UUUU UUG UUUGGAA CG GUUUCUU@LB@ ||:||||| :|||||| ||:||:: : |||: ||| ||||:|| || :||||||@LB@ GUUAGCAU GGUAGAG AAUAAGG G AAAG AAC AAACUUU GC UAAAGAA@LB@3'-AUU...CGUUU G G U A UUU U GAAU AACAA-5'@LB@ | |@LB@ 64 5@LB@@LB@@LB@Energy -17.14 kcal/mol@LB@Hybridization Energy -35.4392 kcal/mol@LB@Unfolding Energy - mRNA 4.76592 kcal/mol@LB@Unfolding Energy - sRNA 13.9327 kcal/mol@LB@@LB@Position - mRNA 214 -- 271@LB@Position - sRNA 6 -- 63@LB@Position Seed - mRNA 265 -- 271@LB@Position Seed - sRNA 6 -- 12@LB@ |
| 87 |
0.003215 |
0.1423 |
bsu04140 |
pbpC |
-8.12 |
0.599206 |
128 -- 139 |
96 -- 107 |
penicillin-binding protein 3 |
|
______________128_____140\n______________|_______|\n___________5'-________AAGGC...CAU-3'\n______________UCUUGUUC\n______________||||:|||\n______________AGAAUAAG\n3'-AUU...GGUAG________GGGUA...CAA-5'\n_____________|________|\n_____________108______95\n |
2 -- 8 |
42 -- 48 |
-8.08477 kcal/mol |
0.903572 kcal/mol |
0.0615861 kcal/mol |
128 140@LB@ | |@LB@ 5'- AAGGC...CAU-3'@LB@ UCUUGUUC@LB@ ||||:|||@LB@ AGAAUAAG@LB@3'-AUU...GGUAG GGGUA...CAA-5'@LB@ | |@LB@ 108 95@LB@@LB@@LB@Energy -8.12 kcal/mol@LB@Hybridization Energy -8.08477 kcal/mol@LB@Unfolding Energy - mRNA 0.903572 kcal/mol@LB@Unfolding Energy - sRNA 0.0615861 kcal/mol@LB@@LB@Position - mRNA 128 -- 139@LB@Position - sRNA 96 -- 107@LB@Position Seed - mRNA 2 -- 8@LB@Position Seed - sRNA 42 -- 48@LB@ |
| 88 |
0.003231 |
0.1423 |
bsu34620 |
mdxD |
-9.76 |
0.344052 |
128 -- 173 |
81 -- 130 |
glucan 1 4-alpha-maltohexaosidase |
|
_____________127____________174\n_____________|______________|\n5'-GUU...AUGCA__GA_______C__GCCAU...GUG-3'\n______________GC__UUCAUCA_CA\n______________:|__|||||||_||\n______________UG__AAGUAGU_GU\n3'-AUU...AAAAA__AG_______U__CCUGU...CAA-5'\n_____________|______________|\n_____________131____________80\n |
220 -- 226 |
158 -- 164 |
-10.4913 kcal/mol |
1.73429 kcal/mol |
1.41528 kcal/mol |
127 174@LB@ | |@LB@5'-GUU...AUGCA GA C GCCAU...GUG-3'@LB@ GC UUCAUCA CA@LB@ :| ||||||| ||@LB@ UG AAGUAGU GU@LB@3'-AUU...AAAAA AG U CCUGU...CAA-5'@LB@ | |@LB@ 131 80@LB@@LB@@LB@Energy -9.76 kcal/mol@LB@Hybridization Energy -10.4913 kcal/mol@LB@Unfolding Energy - mRNA 1.73429 kcal/mol@LB@Unfolding Energy - sRNA 1.41528 kcal/mol@LB@@LB@Position - mRNA 128 -- 173@LB@Position - sRNA 81 -- 130@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 158 -- 164@LB@ |
| 89 |
0.003315 |
0.1443 |
bsu30460 |
ytrA |
-11.86 |
0.143485 |
75 -- 137 |
74 -- 137 |
GntR family transcriptional regulator |
|
_____________74__________________________________________________________________138\n_____________|___________________________________________________________________|\n5'-UUU...UGAUU_______________AUCGC_____CU_______U_G___UU________UC_______AAUG____AAAAG...AUA-3'\n______________GCGGCA___UCUGUG_____UUGUU__UUUUUUC_U_UUC___GUGAUGA__GGUUUUG____GCAA\n______________||:|||___||::|:_____||||:__:|||:||_|_|||___||:||||__||:||:|____||||\n______________CGUCGU___AGGUAU_____AACAG__GAAAGAG_A_AAG___CAUUACU__CCGAAGC____CGUU\n3'-AUU...ACAGG______UCA______AUUAC_____U________U_G___UUU_______UA_______AA______AGAAC...CAA-5'\n_____________|___________________________________________________________________|\n_____________138_________________________________________________________________73\n |
123 -- 129 |
80 -- 86 |
-41.3024 kcal/mol |
16.5538 kcal/mol |
14.8582 kcal/mol |
74 138@LB@ | |@LB@5'-UUU...UGAUU AUCGC CU U G UU UC AAUG AAAAG...AUA-3'@LB@ GCGGCA UCUGUG UUGUU UUUUUUC U UUC GUGAUGA GGUUUUG GCAA@LB@ ||:||| ||::|: ||||: :|||:|| | ||| ||:|||| ||:||:| ||||@LB@ CGUCGU AGGUAU AACAG GAAAGAG A AAG CAUUACU CCGAAGC CGUU@LB@3'-AUU...ACAGG UCA AUUAC U U G UUU UA AA AGAAC...CAA-5'@LB@ | |@LB@ 138 73@LB@@LB@@LB@Energy -11.86 kcal/mol@LB@Hybridization Energy -41.3024 kcal/mol@LB@Unfolding Energy - mRNA 16.5538 kcal/mol@LB@Unfolding Energy - sRNA 14.8582 kcal/mol@LB@@LB@Position - mRNA 75 -- 137@LB@Position - sRNA 74 -- 137@LB@Position Seed - mRNA 123 -- 129@LB@Position Seed - sRNA 80 -- 86@LB@ |
| 90 |
0.003471 |
0.1486 |
bsu35950 |
rbsC |
-15.06 |
0.032267 |
242 -- 293 |
40 -- 84 |
ribose transport system permease RbsC |
|
_____________241______________________________________294\n_____________|________________________________________|\n5'-CAA...AUUCA_____AC_G____AU______AAC________________GUUUA...UCA-3'\n______________CUUCG__G_AGUC___GCAAA___UCG_GCCCGUUUCUUG\n______________|||||__|_|:||___|||||___|||_:|||:|:||||:\n______________GAAGC__C_UUAG___CGUUU___AGC_UGGGUAGAGAAU\n3'-AUU...UUACC_____AA_G____AAC_____GUU___A____________AAGGG...CAA-5'\n_____________|________________________________________|\n_____________85_______________________________________39\n |
273 -- 279 |
45 -- 51 |
-33.8346 kcal/mol |
9.61657 kcal/mol |
8.57091 kcal/mol |
241 294@LB@ | |@LB@5'-CAA...AUUCA AC G AU AAC GUUUA...UCA-3'@LB@ CUUCG G AGUC GCAAA UCG GCCCGUUUCUUG@LB@ ||||| | |:|| ||||| ||| :|||:|:||||:@LB@ GAAGC C UUAG CGUUU AGC UGGGUAGAGAAU@LB@3'-AUU...UUACC AA G AAC GUU A AAGGG...CAA-5'@LB@ | |@LB@ 85 39@LB@@LB@@LB@Energy -15.06 kcal/mol@LB@Hybridization Energy -33.8346 kcal/mol@LB@Unfolding Energy - mRNA 9.61657 kcal/mol@LB@Unfolding Energy - sRNA 8.57091 kcal/mol@LB@@LB@Position - mRNA 242 -- 293@LB@Position - sRNA 40 -- 84@LB@Position Seed - mRNA 273 -- 279@LB@Position Seed - sRNA 45 -- 51@LB@ |
