Input and runtime details for job 1786318 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1786318 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:20:27 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:20:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:01:46 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:01:53 CET 2018
	Job Completed		@ Fri Feb 16 22:02:22 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1786318&toolName=CopraRNA ( 30 days expiry )

Description of the job

ArcZ

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
81	0.004478	0.1855	b3327	gspF	-8.77	0.233220	243 -- 251	26 -- 34	general secretory pathway component cryptic	_____________242_______252\n_____________\|_________\|\n5'-AUG...AAAAA_________AUCAU...AAG-3'\n______________UUGCAAGGG\n______________\|\|:\|\|\|\|\|\|\n______________AAUGUUCCC\n3'-UUU...UACUC_________GUGUC...GUG-5'\n_____________\|_________\|\n_____________35________25\n	245 -- 251	26 -- 32	-12.3281 kcal/mol	0.874927 kcal/mol	2.67841 kcal/mol	242 252@LB@ \| \|@LB@5'-AUG...AAAAA AUCAU...AAG-3'@LB@ UUGCAAGGG@LB@ \|\|:\|\|\|\|\|\|@LB@ AAUGUUCCC@LB@3'-UUU...UACUC GUGUC...GUG-5'@LB@ \| \|@LB@ 35 25@LB@@LB@@LB@Energy -8.77 kcal/mol@LB@Hybridization Energy -12.3281 kcal/mol@LB@Unfolding Energy - mRNA 0.874927 kcal/mol@LB@Unfolding Energy - sRNA 2.67841 kcal/mol@LB@@LB@Position - mRNA 243 -- 251@LB@Position - sRNA 26 -- 34@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 26 -- 32@LB@
82	0.004565	0.1855	b4144	yjeI	-10.12	0.132253	163 -- 240	1 -- 93	DUF4156 family lipoprotein	_____________162_____________________________________________________________________________________________________241\n_____________\|_______________________________________________________________________________________________________\|\n5'-GCA...GACGA_____________U_________UUGAUUAU_______AACA_____A_______A________________UUA____G_______________U_______UACUG...GCA-3'\n______________CGCGAGUG__GCG_CCAGC_UCA________GGGGAAU______UGC__CGUAAA______________UAC____GCA_GG_AUUG____UCGG_GCCGCGC\n______________\|\|\|\|\|:\|:__\|\|\|_\|\|\|:\|_:\|\|________\|\|\|:\|\|\|______\|\|\|__\|\|\|\|\|\|______________\|\|\|____\|\|\|_:\|_\|:\|\|____\|\|:\|_\|\|\|\|\|:\|\n______________GCGCUUAU__CGC_GGUUG_GGU________CCCUUUA______ACG__GCAUUU______________AUG____CGU_UC_UGAC____AGUC_CGGCGUG\n3'-UUU...CCCAC________GA_________U__________________GAACCG___CG______AAUAAUACUACUCA___UUCC___G__G____AAAA____________-5'\n_____________\|______________________________________________________________________________________________________\|\n_____________94_____________________________________________________________________________________________________1\n	234 -- 240	1 -- 7	-61.0049 kcal/mol	21.8834 kcal/mol	29.0051 kcal/mol	162 241@LB@ \| \|@LB@5'-GCA...GACGA U UUGAUUAU AACA A A UUA G U UACUG...GCA-3'@LB@ CGCGAGUG GCG CCAGC UCA GGGGAAU UGC CGUAAA UAC GCA GG AUUG UCGG GCCGCGC@LB@ \|\|\|\|\|:\|: \|\|\| \|\|\|:\| :\|\| \|\|\|:\|\|\| \|\|\| \|\|\|\|\|\| \|\|\| \|\|\| :\| \|:\|\| \|\|:\| \|\|\|\|\|:\|@LB@ GCGCUUAU CGC GGUUG GGU CCCUUUA ACG GCAUUU AUG CGU UC UGAC AGUC CGGCGUG@LB@3'-UUU...CCCAC GA U GAACCG CG AAUAAUACUACUCA UUCC G G AAAA -5'@LB@ \| \|@LB@ 94 1@LB@@LB@@LB@Energy -10.12 kcal/mol@LB@Hybridization Energy -61.0049 kcal/mol@LB@Unfolding Energy - mRNA 21.8834 kcal/mol@LB@Unfolding Energy - sRNA 29.0051 kcal/mol@LB@@LB@Position - mRNA 163 -- 240@LB@Position - sRNA 1 -- 93@LB@Position Seed - mRNA 234 -- 240@LB@Position Seed - sRNA 1 -- 7@LB@
83	0.004611	0.1855	b1593	ynfK	-13.15	0.034145	276 -- 295	66 -- 83	putative dethiobiotin synthetase	_____________275__________________296\n_____________\|____________________\|\n5'-CAC...UACAA________CUC_________AACGG-3'\n______________GCGUUAGC___CCAGGGAAA\n______________\|\|\|::\|:\|___\|\|\|\|\|\|\|\|\|\n______________CGCGGUUG___GGUCCCUUU\n3'-UUU...UAUGA________U___________AGAAC...GUG-5'\n_____________\|____________________\|\n_____________84___________________65\n	289 -- 295	66 -- 72	-26.3491 kcal/mol	5.84339 kcal/mol	7.35847 kcal/mol	275 296@LB@ \| \|@LB@5'-CAC...UACAA CUC AACGG-3'@LB@ GCGUUAGC CCAGGGAAA@LB@ \|\|\|::\|:\| \|\|\|\|\|\|\|\|\|@LB@ CGCGGUUG GGUCCCUUU@LB@3'-UUU...UAUGA U AGAAC...GUG-5'@LB@ \| \|@LB@ 84 65@LB@@LB@@LB@Energy -13.15 kcal/mol@LB@Hybridization Energy -26.3491 kcal/mol@LB@Unfolding Energy - mRNA 5.84339 kcal/mol@LB@Unfolding Energy - sRNA 7.35847 kcal/mol@LB@@LB@Position - mRNA 276 -- 295@LB@Position - sRNA 66 -- 83@LB@Position Seed - mRNA 289 -- 295@LB@Position Seed - sRNA 66 -- 72@LB@
84	0.004676	0.1855	b0526	cysS	-14.43	0.019007	256 -- 269	64 -- 77	cysteinyl-tRNA synthetase	_____________255____________270\n_____________\|______________\|\n5'-GCC...UAUUC______________GGCAU...ACG-3'\n______________ACGCCGGGGAAGUC\n______________\|\|:\|\|:\|\|\|\|\|:\|\|\n______________UGUGGUCCCUUUAG\n3'-UUU...GCGGU______________AACCG...GUG-5'\n_____________\|______________\|\n_____________78_____________63\n	263 -- 269	64 -- 70	-23.2043 kcal/mol	4.03864 kcal/mol	4.72709 kcal/mol	255 270@LB@ \| \|@LB@5'-GCC...UAUUC GGCAU...ACG-3'@LB@ ACGCCGGGGAAGUC@LB@ \|\|:\|\|:\|\|\|\|\|:\|\|@LB@ UGUGGUCCCUUUAG@LB@3'-UUU...GCGGU AACCG...GUG-5'@LB@ \| \|@LB@ 78 63@LB@@LB@@LB@Energy -14.43 kcal/mol@LB@Hybridization Energy -23.2043 kcal/mol@LB@Unfolding Energy - mRNA 4.03864 kcal/mol@LB@Unfolding Energy - sRNA 4.72709 kcal/mol@LB@@LB@Position - mRNA 256 -- 269@LB@Position - sRNA 64 -- 77@LB@Position Seed - mRNA 263 -- 269@LB@Position Seed - sRNA 64 -- 70@LB@
85	0.004761	0.1855	b2077	iceT	-11.46	0.073016	53 -- 74	64 -- 86	putative citrate/iron-citrate/zinc-citrate efflux transporter	_____________52______________________75\n_____________\|_______________________\|\n5'-CGG...UCGGA______G_______CU_______ACCAU...UUC-3'\n______________GCUGCG_CAAC_CC__GGGGAUC\n______________:\|\|\|\|\|_\|\|\|\|_\|\|__\|\|::\|\|\|\n______________UGACGC_GUUG_GG__CCUUUAG\n3'-UUU...GCUUA______G____U__UC_______AACCG...GUG-5'\n_____________\|_______________________\|\n_____________87______________________63\n	68 -- 74	64 -- 70	-29.0378 kcal/mol	10.1908 kcal/mol	7.38435 kcal/mol	52 75@LB@ \| \|@LB@5'-CGG...UCGGA G CU ACCAU...UUC-3'@LB@ GCUGCG CAAC CC GGGGAUC@LB@ :\|\|\|\|\| \|\|\|\| \|\| \|\|::\|\|\|@LB@ UGACGC GUUG GG CCUUUAG@LB@3'-UUU...GCUUA G U UC AACCG...GUG-5'@LB@ \| \|@LB@ 87 63@LB@@LB@@LB@Energy -11.46 kcal/mol@LB@Hybridization Energy -29.0378 kcal/mol@LB@Unfolding Energy - mRNA 10.1908 kcal/mol@LB@Unfolding Energy - sRNA 7.38435 kcal/mol@LB@@LB@Position - mRNA 53 -- 74@LB@Position - sRNA 64 -- 86@LB@Position Seed - mRNA 68 -- 74@LB@Position Seed - sRNA 64 -- 70@LB@
86	0.004777	0.1855	b3157	yhbT	-11.83	0.061936	277 -- 294	70 -- 85	SCP-2 sterol transfer family protein	_____________276________________295\n_____________\|__________________\|\n5'-CUG...GCCAU________AA________UUCUUG-3'\n______________UUGCGCUA__ACGCCAGG\n______________:\|\|\|\|\|:\|__\|\|:\|\|\|\|\|\n______________GACGCGGU__UGUGGUCC\n3'-UUU...CUUAU__________________CUUUA...GUG-5'\n_____________\|__________________\|\n_____________86_________________69\n	288 -- 294	70 -- 76	-24.8001 kcal/mol	5.69349 kcal/mol	7.27689 kcal/mol	276 295@LB@ \| \|@LB@5'-CUG...GCCAU AA UUCUUG-3'@LB@ UUGCGCUA ACGCCAGG@LB@ :\|\|\|\|\|:\| \|\|:\|\|\|\|\|@LB@ GACGCGGU UGUGGUCC@LB@3'-UUU...CUUAU CUUUA...GUG-5'@LB@ \| \|@LB@ 86 69@LB@@LB@@LB@Energy -11.83 kcal/mol@LB@Hybridization Energy -24.8001 kcal/mol@LB@Unfolding Energy - mRNA 5.69349 kcal/mol@LB@Unfolding Energy - sRNA 7.27689 kcal/mol@LB@@LB@Position - mRNA 277 -- 294@LB@Position - sRNA 70 -- 85@LB@Position Seed - mRNA 288 -- 294@LB@Position Seed - sRNA 70 -- 76@LB@
87	0.004792	0.1855	b0476	aes	-9.71	0.157345	34 -- 55	56 -- 76	acetyl esterase	_____________33______________________56\n_____________\|_______________________\|\n5'-CGU...ACGUU______________CA_______UUUUA...CAA-3'\n______________UACC_GGGAAAUUU__UGGUUGU\n______________:\|\|\|_\|\|\|\|\|\|\|:\|__\|\|\|:\|\|:\n______________GUGG_CCCUUUAGA__ACCGACG\n3'-UUU...CGGUU____U__________________CGGCA...GUG-5'\n_____________\|_______________________\|\n_____________77______________________55\n	49 -- 55	56 -- 62	-24.1621 kcal/mol	6.04137 kcal/mol	8.40711 kcal/mol	33 56@LB@ \| \|@LB@5'-CGU...ACGUU CA UUUUA...CAA-3'@LB@ UACC GGGAAAUUU UGGUUGU@LB@ :\|\|\| \|\|\|\|\|\|\|:\| \|\|\|:\|\|:@LB@ GUGG CCCUUUAGA ACCGACG@LB@3'-UUU...CGGUU U CGGCA...GUG-5'@LB@ \| \|@LB@ 77 55@LB@@LB@@LB@Energy -9.71 kcal/mol@LB@Hybridization Energy -24.1621 kcal/mol@LB@Unfolding Energy - mRNA 6.04137 kcal/mol@LB@Unfolding Energy - sRNA 8.40711 kcal/mol@LB@@LB@Position - mRNA 34 -- 55@LB@Position - sRNA 56 -- 76@LB@Position Seed - mRNA 49 -- 55@LB@Position Seed - sRNA 56 -- 62@LB@
88	0.004799	0.1855	b2213	ada	-9.32	0.185618	222 -- 245	63 -- 86	fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase	_____________221________________________246\n_____________\|__________________________\|\n5'-GGA...GCUUA____A__A__________C_______GUCUU...CCA-3'\n______________ACUG_CG_UCAACGCUGG___AAUCU\n______________\|\|\|\|_\|\|_:\|\|\|\|:\|::\|___\|\|\|\|\|\n______________UGAC_GC_GGUUGUGGUC___UUAGA\n3'-UUU...GCUUA__________________CCU_____ACCGA...GUG-5'\n_____________\|__________________________\|\n_____________87_________________________62\n	233 -- 239	71 -- 77	-21.7615 kcal/mol	5.05104 kcal/mol	7.38602 kcal/mol	221 246@LB@ \| \|@LB@5'-GGA...GCUUA A A C GUCUU...CCA-3'@LB@ ACUG CG UCAACGCUGG AAUCU@LB@ \|\|\|\| \|\| :\|\|\|\|:\|::\| \|\|\|\|\|@LB@ UGAC GC GGUUGUGGUC UUAGA@LB@3'-UUU...GCUUA CCU ACCGA...GUG-5'@LB@ \| \|@LB@ 87 62@LB@@LB@@LB@Energy -9.32 kcal/mol@LB@Hybridization Energy -21.7615 kcal/mol@LB@Unfolding Energy - mRNA 5.05104 kcal/mol@LB@Unfolding Energy - sRNA 7.38602 kcal/mol@LB@@LB@Position - mRNA 222 -- 245@LB@Position - sRNA 63 -- 86@LB@Position Seed - mRNA 233 -- 239@LB@Position Seed - sRNA 71 -- 77@LB@
89	0.004811	0.1855	b4336	yjiN	-13.14	0.034257	120 -- 134	70 -- 84	zinc-type alcohol dehydrogenase-like protein	_____________119_____________135\n_____________\|_______________\|\n5'-GGU...UCGCG_______________CAGCC...CCA-3'\n______________UGCGCCAGCAUCAGG\n______________\|\|\|\|\|\|\|:\|\|:\|\|\|\|\n______________ACGCGGUUGUGGUCC\n3'-UUU...UUAUG_______________CUUUA...GUG-5'\n_____________\|_______________\|\n_____________85______________69\n	128 -- 134	70 -- 76	-28.7852 kcal/mol	8.39668 kcal/mol	7.24859 kcal/mol	119 135@LB@ \| \|@LB@5'-GGU...UCGCG CAGCC...CCA-3'@LB@ UGCGCCAGCAUCAGG@LB@ \|\|\|\|\|\|\|:\|\|:\|\|\|\|@LB@ ACGCGGUUGUGGUCC@LB@3'-UUU...UUAUG CUUUA...GUG-5'@LB@ \| \|@LB@ 85 69@LB@@LB@@LB@Energy -13.14 kcal/mol@LB@Hybridization Energy -28.7852 kcal/mol@LB@Unfolding Energy - mRNA 8.39668 kcal/mol@LB@Unfolding Energy - sRNA 7.24859 kcal/mol@LB@@LB@Position - mRNA 120 -- 134@LB@Position - sRNA 70 -- 84@LB@Position Seed - mRNA 128 -- 134@LB@Position Seed - sRNA 70 -- 76@LB@
90	0.004828	0.1855	b2149	mglA	-7.32	0.409352	216 -- 239	61 -- 86	methyl-galactoside ABC transporter ATPase	_____________215________________________240\n_____________\|__________________________\|\n5'-CGA...CAACG___C____CU__________UA____UUGGA...AUU-3'\n______________ACU_CGUC____CCGGGGAA__CUUG\n______________\|\|\|_\|\|:\|____\|\|:\|\|\|\|\|__\|\|\|\|\n______________UGA_GCGG____GGUCCCUU__GAAC\n3'-UUU...GCUUA___C____UUGU________UA____CGACG...GUG-5'\n_____________\|__________________________\|\n_____________87_________________________60\n	227 -- 233	67 -- 73	-23.5814 kcal/mol	8.829 kcal/mol	7.43637 kcal/mol	215 240@LB@ \| \|@LB@5'-CGA...CAACG C CU UA UUGGA...AUU-3'@LB@ ACU CGUC CCGGGGAA CUUG@LB@ \|\|\| \|\|:\| \|\|:\|\|\|\|\| \|\|\|\|@LB@ UGA GCGG GGUCCCUU GAAC@LB@3'-UUU...GCUUA C UUGU UA CGACG...GUG-5'@LB@ \| \|@LB@ 87 60@LB@@LB@@LB@Energy -7.32 kcal/mol@LB@Hybridization Energy -23.5814 kcal/mol@LB@Unfolding Energy - mRNA 8.829 kcal/mol@LB@Unfolding Energy - sRNA 7.43637 kcal/mol@LB@@LB@Position - mRNA 216 -- 239@LB@Position - sRNA 61 -- 86@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 67 -- 73@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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