| 71 |
0.002326 |
0.1242 |
bsu37230 |
ywjA |
-17.78 |
0.008577 |
195 -- 260 |
99 -- 147 |
ABC transporter ATP-binding protein |
|
_____________194___________________________________________________________261\n_____________|_____________________________________________________________|\n5'-UGA...GGGAA____GG__G_________U__UUUUCAUAUUAUAAA________AAC______________GACUU...UUC-3'\n______________GAUA__GU_CUGCGGCAA_UU_______________CCAUAUAA___GUUGUU_CUUUCUC\n______________||:|__||_|:||:||||_||_______________||||||||___|||||:_|||||||\n______________CUGU__CA_GGCGUCGUU_AA_______________GGUAUAUU___CAACAG_GAAAGAG\n3'-AUU...UGUAA____AA____________C_________________________A________U_______UAGAA...CAA-5'\n_____________|_____________________________________________________________|\n_____________148___________________________________________________________98\n |
248 -- 254 |
105 -- 111 |
-39.3136 kcal/mol |
10.514 kcal/mol |
13.0974 kcal/mol |
194 261@LB@ | |@LB@5'-UGA...GGGAA GG G U UUUUCAUAUUAUAAA AAC GACUU...UUC-3'@LB@ GAUA GU CUGCGGCAA UU CCAUAUAA GUUGUU CUUUCUC@LB@ ||:| || |:||:|||| || |||||||| |||||: |||||||@LB@ CUGU CA GGCGUCGUU AA GGUAUAUU CAACAG GAAAGAG@LB@3'-AUU...UGUAA AA C A U UAGAA...CAA-5'@LB@ | |@LB@ 148 98@LB@@LB@@LB@Energy -17.78 kcal/mol@LB@Hybridization Energy -39.3136 kcal/mol@LB@Unfolding Energy - mRNA 10.514 kcal/mol@LB@Unfolding Energy - sRNA 13.0974 kcal/mol@LB@@LB@Position - mRNA 195 -- 260@LB@Position - sRNA 99 -- 147@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 105 -- 111@LB@ |
| 72 |
0.002326 |
0.1242 |
bsu10730 |
yisI |
-10.35 |
0.272902 |
44 -- 108 |
96 -- 182 |
aspartyl-phosphate phosphatase YisI |
|
_____________43___________________________________109\n_____________|____________________________________|\n5'-UAG...AAAGG_________AAA________________________GUCAA...UGG-3'\n______________CCGCAGCAA_____________UUGUCGCUUUUUUA\n______________|||||||||_____________|||||:||||:|:|\n______________GGCGUCGUU_____________AACAGUGAAAGAGU\n3'-AUU...UAACA_________CAAGGUAUAUUAC______________AGAAG...CAA-5'\n_____________|____________________________________|\n_____________183__________________________________95\n |
90 -- 96 |
110 -- 116 |
-28.3207 kcal/mol |
8.15126 kcal/mol |
10.3722 kcal/mol |
43 109@LB@ | |@LB@5'-UAG...AAAGG AAA GUCAA...UGG-3'@LB@ CCGCAGCAA UUGUCGCUUUUUUA@LB@ ||||||||| |||||:||||:|:|@LB@ GGCGUCGUU AACAGUGAAAGAGU@LB@3'-AUU...UAACA CAAGGUAUAUUAC AGAAG...CAA-5'@LB@ | |@LB@ 183 95@LB@@LB@@LB@Energy -10.35 kcal/mol@LB@Hybridization Energy -28.3207 kcal/mol@LB@Unfolding Energy - mRNA 8.15126 kcal/mol@LB@Unfolding Energy - sRNA 10.3722 kcal/mol@LB@@LB@Position - mRNA 44 -- 108@LB@Position - sRNA 96 -- 182@LB@Position Seed - mRNA 90 -- 96@LB@Position Seed - sRNA 110 -- 116@LB@ |
| 73 |
0.002371 |
0.1242 |
bsu00930 |
cysE |
-20.00 |
0.002833 |
192 -- 291 |
102 -- 187 |
serine acetyltransferase |
|
_____________191__________________________________________________________________________292\n_____________|____________________________________________________________________________|\n5'-CCU...AAUGC____AGAA__________C___GUUCGAUCAAGAUC__________AAG_____UU__AA__G_____AA______CCGGU...UAC-3'\n______________UCAA____GACAUUGAUA_UGU______________CCGCAGCAAG___C_UAU__UG__GU_AUUUU__CUUAUU\n______________||||____||||||||:|_|||______________||||||||||___|_|||__||__||_|:|||__||:||:\n______________AGUU____CUGUAACUGU_ACA______________GGCGUCGUUC___G_AUA__AC__CA_UGAAA__GAGUAG\n3'-AUU...GAAGU____GUC___________A___________________________AAG_U___UU__AA__G_____________AAGUU...CAA-5'\n_____________|____________________________________________________________________________|\n_____________188__________________________________________________________________________101\n |
256 -- 262 |
130 -- 136 |
-42.0244 kcal/mol |
15.3231 kcal/mol |
14.7636 kcal/mol |
191 292@LB@ | |@LB@5'-CCU...AAUGC AGAA C GUUCGAUCAAGAUC AAG UU AA G AA CCGGU...UAC-3'@LB@ UCAA GACAUUGAUA UGU CCGCAGCAAG C UAU UG GU AUUUU CUUAUU@LB@ |||| ||||||||:| ||| |||||||||| | ||| || || |:||| ||:||:@LB@ AGUU CUGUAACUGU ACA GGCGUCGUUC G AUA AC CA UGAAA GAGUAG@LB@3'-AUU...GAAGU GUC A AAG U UU AA G AAGUU...CAA-5'@LB@ | |@LB@ 188 101@LB@@LB@@LB@Energy -20.00 kcal/mol@LB@Hybridization Energy -42.0244 kcal/mol@LB@Unfolding Energy - mRNA 15.3231 kcal/mol@LB@Unfolding Energy - sRNA 14.7636 kcal/mol@LB@@LB@Position - mRNA 192 -- 291@LB@Position - sRNA 102 -- 187@LB@Position Seed - mRNA 256 -- 262@LB@Position Seed - sRNA 130 -- 136@LB@ |
| 74 |
0.002384 |
0.1242 |
bsu09450 |
yhdF |
-14.81 |
0.036487 |
208 -- 244 |
132 -- 159 |
oxidoreductase |
|
_____________207____________________________________245\n_____________|______________________________________|\n5'-UCC...GAACC__________GACAAACAGAAG____AGAA________UCAGG...AGG-3'\n______________CAAU_GGACA____________GACA____CCGCAGCA\n______________|||:_|||||____________||||____||||||||\n______________GUUG_CCUGU____________CUGU____GGCGUCGU\n3'-AUU...AAGUA____U_____AA______________AACA________UCAAG...CAA-5'\n_____________|______________________________________|\n_____________160____________________________________131\n |
238 -- 244 |
132 -- 138 |
-26.7883 kcal/mol |
3.14586 kcal/mol |
9.22644 kcal/mol |
207 245@LB@ | |@LB@5'-UCC...GAACC GACAAACAGAAG AGAA UCAGG...AGG-3'@LB@ CAAU GGACA GACA CCGCAGCA@LB@ |||: ||||| |||| ||||||||@LB@ GUUG CCUGU CUGU GGCGUCGU@LB@3'-AUU...AAGUA U AA AACA UCAAG...CAA-5'@LB@ | |@LB@ 160 131@LB@@LB@@LB@Energy -14.81 kcal/mol@LB@Hybridization Energy -26.7883 kcal/mol@LB@Unfolding Energy - mRNA 3.14586 kcal/mol@LB@Unfolding Energy - sRNA 9.22644 kcal/mol@LB@@LB@Position - mRNA 208 -- 244@LB@Position - sRNA 132 -- 159@LB@Position Seed - mRNA 238 -- 244@LB@Position Seed - sRNA 132 -- 138@LB@ |
| 75 |
0.002405 |
0.1242 |
bsu01600 |
ybbA |
-13.26 |
0.075734 |
128 -- 177 |
34 -- 77 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 12354229 2974033; Product type pe: enzyme' |
|
_____________127______________________________________________178\n_____________|________________________________________________|\n5'-UCA...CGCAG___UC_________________________GGA_______________AUAUC...UUU-3'\n______________GCA__AUUUUGGCG_________AUCC_UC____GUUCCUUAUUUCUU\n______________|||__||:|||||:_________|:||_||____:||||::|||||||\n______________CGU__UAGAACCGU_________UGGG_AG____UAAGGGGUAAAGAA\n3'-AUU...AGCAA______________UUGUUAGCA____U__AGAA______________ACUUU...CAA-5'\n_____________|________________________________________________|\n_____________78_______________________________________________33\n |
158 -- 164 |
32 -- 38 |
-31.1473 kcal/mol |
6.80918 kcal/mol |
13.8943 kcal/mol |
127 178@LB@ | |@LB@5'-UCA...CGCAG UC GGA AUAUC...UUU-3'@LB@ GCA AUUUUGGCG AUCC UC GUUCCUUAUUUCUU@LB@ ||| ||:|||||: |:|| || :||||::|||||||@LB@ CGU UAGAACCGU UGGG AG UAAGGGGUAAAGAA@LB@3'-AUU...AGCAA UUGUUAGCA U AGAA ACUUU...CAA-5'@LB@ | |@LB@ 78 33@LB@@LB@@LB@Energy -13.26 kcal/mol@LB@Hybridization Energy -31.1473 kcal/mol@LB@Unfolding Energy - mRNA 6.80918 kcal/mol@LB@Unfolding Energy - sRNA 13.8943 kcal/mol@LB@@LB@Position - mRNA 128 -- 177@LB@Position - sRNA 34 -- 77@LB@Position Seed - mRNA 158 -- 164@LB@Position Seed - sRNA 32 -- 38@LB@ |
| 76 |
0.002466 |
0.1257 |
bsu07870 |
yfkK |
-15.12 |
0.031390 |
12 -- 33 |
33 -- 55 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________11______________________34\n_____________|_______________________|\n5'-UGU...ACCUC__________UGC__________CUAAA...UCA-3'\n______________CCUA_UUCUU___CCCUAUUUCU\n______________||:|_:||||___|||:||||||\n______________GGGU_GAGAA___GGGGUAAAGA\n3'-AUU...AGCAU____A_____UAA__________AACUU...CAA-5'\n_____________|_______________________|\n_____________56______________________32\n |
27 -- 33 |
33 -- 39 |
-21.8682 kcal/mol |
0.689294 kcal/mol |
9.04338 kcal/mol |
11 34@LB@ | |@LB@5'-UGU...ACCUC UGC CUAAA...UCA-3'@LB@ CCUA UUCUU CCCUAUUUCU@LB@ ||:| :|||| |||:||||||@LB@ GGGU GAGAA GGGGUAAAGA@LB@3'-AUU...AGCAU A UAA AACUU...CAA-5'@LB@ | |@LB@ 56 32@LB@@LB@@LB@Energy -15.12 kcal/mol@LB@Hybridization Energy -21.8682 kcal/mol@LB@Unfolding Energy - mRNA 0.689294 kcal/mol@LB@Unfolding Energy - sRNA 9.04338 kcal/mol@LB@@LB@Position - mRNA 12 -- 33@LB@Position - sRNA 33 -- 55@LB@Position Seed - mRNA 27 -- 33@LB@Position Seed - sRNA 33 -- 39@LB@ |
| 77 |
0.002638 |
0.1315 |
bsu36790 |
ywmA |
-11.30 |
0.183092 |
1 -- 48 |
33 -- 81 |
Evidence 5: No homology to any previously reported sequences |
|
_____________0_______________49\n_____________|_______________|\n5'-CGA...GUUCA_______________AUAUA...GAC-3'\n______________UACCC_UUUCUUAUU\n______________|||||_|:|||||||\n______________AUGGG_AGAGAAUAA\n3'-AUU...UUAGC_____U_________GGGGU...CAA-5'\n_____________|_______________|\n_____________82______________32\n |
65 -- 71 |
43 -- 49 |
-14.8917 kcal/mol |
3.00255 kcal/mol |
4.80466 kcal/mol |
0 49@LB@ | |@LB@5'-CGA...GUUCA AUAUA...GAC-3'@LB@ UACCC UUUCUUAUU@LB@ ||||| |:|||||||@LB@ AUGGG AGAGAAUAA@LB@3'-AUU...UUAGC U GGGGU...CAA-5'@LB@ | |@LB@ 82 32@LB@@LB@@LB@Energy -11.30 kcal/mol@LB@Hybridization Energy -14.8917 kcal/mol@LB@Unfolding Energy - mRNA 3.00255 kcal/mol@LB@Unfolding Energy - sRNA 4.80466 kcal/mol@LB@@LB@Position - mRNA 1 -- 48@LB@Position - sRNA 33 -- 81@LB@Position Seed - mRNA 65 -- 71@LB@Position Seed - sRNA 43 -- 49@LB@ |
| 78 |
0.002646 |
0.1315 |
bsu37410 |
albE |
-16.69 |
0.014616 |
33 -- 81 |
32 -- 77 |
antilisterial bacteriocin subtilosin biosynthesis protein AlbE |
|
_____________32_________________________________________________82\n_____________|__________________________________________________|\n5'-CUU...AUGGA_______CU_____AU____GU_______G__UUGUC______UA_____AGCCA...AAA-3'\n______________GCGGUUU___CAAA__AUCG__CUUAUCU_UU_____UUCCCC__UUCUU\n______________||::|:|___||||__||||__|::||||_||_____||||||__|||||\n______________CGUUAGA___GUUU__UAGC__GGGUAGA_AA_____AAGGGG__AAGAA\n3'-AUU...AGCAA_______ACC____GU____AU_______G__U__________UA_____ACUUU...CAA-5'\n_____________|__________________________________________________|\n_____________78_________________________________________________31\n |
54 -- 60 |
49 -- 55 |
-34.8992 kcal/mol |
7.30498 kcal/mol |
13.8943 kcal/mol |
32 82@LB@ | |@LB@5'-CUU...AUGGA CU AU GU G UUGUC UA AGCCA...AAA-3'@LB@ GCGGUUU CAAA AUCG CUUAUCU UU UUCCCC UUCUU@LB@ ||::|:| |||| |||| |::|||| || |||||| |||||@LB@ CGUUAGA GUUU UAGC GGGUAGA AA AAGGGG AAGAA@LB@3'-AUU...AGCAA ACC GU AU G U UA ACUUU...CAA-5'@LB@ | |@LB@ 78 31@LB@@LB@@LB@Energy -16.69 kcal/mol@LB@Hybridization Energy -34.8992 kcal/mol@LB@Unfolding Energy - mRNA 7.30498 kcal/mol@LB@Unfolding Energy - sRNA 13.8943 kcal/mol@LB@@LB@Position - mRNA 33 -- 81@LB@Position - sRNA 32 -- 77@LB@Position Seed - mRNA 54 -- 60@LB@Position Seed - sRNA 49 -- 55@LB@ |
| 79 |
0.002683 |
0.1316 |
bsu12250 |
yjkB |
-15.23 |
0.029777 |
154 -- 245 |
42 -- 131 |
ABC transporter ATP-binding protein |
|
_____________153__________________________________________________________246\n_____________|____________________________________________________________|\n5'-AUA...UGAAA___GA__A_______GGAA__________AAACACAAAACAUACC_G_______C_____AGAUC...AGG-3'\n______________GGU__UU_UUGUAAU____GCAAACGAUC________________A_CCAUCUC___UUC\n______________||:__|:_|||:|||____||||||:|||________________|_|||||||___|||\n______________CCG__AG_AACGUUA____CGUUUGUUAG________________U_GGUAGAG___AAG\n3'-AUU...ACUUA___A___C_______GAAC__________CA_______________G_______AAU___GGGUA...CAA-5'\n_____________|____________________________________________________________|\n_____________132__________________________________________________________41\n |
235 -- 241 |
48 -- 54 |
-30.736 kcal/mol |
10.3673 kcal/mol |
9.19615 kcal/mol |
153 246@LB@ | |@LB@5'-AUA...UGAAA GA A GGAA AAACACAAAACAUACC G C AGAUC...AGG-3'@LB@ GGU UU UUGUAAU GCAAACGAUC A CCAUCUC UUC@LB@ ||: |: |||:||| ||||||:||| | ||||||| |||@LB@ CCG AG AACGUUA CGUUUGUUAG U GGUAGAG AAG@LB@3'-AUU...ACUUA A C GAAC CA G AAU GGGUA...CAA-5'@LB@ | |@LB@ 132 41@LB@@LB@@LB@Energy -15.23 kcal/mol@LB@Hybridization Energy -30.736 kcal/mol@LB@Unfolding Energy - mRNA 10.3673 kcal/mol@LB@Unfolding Energy - sRNA 9.19615 kcal/mol@LB@@LB@Position - mRNA 154 -- 245@LB@Position - sRNA 42 -- 131@LB@Position Seed - mRNA 235 -- 241@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 80 |
0.002732 |
0.1323 |
bsu14790 |
ylaI |
-9.42 |
0.391167 |
138 -- 189 |
118 -- 178 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________137____________________________________________________190\n_____________|______________________________________________________|\n5'-AGA...CUUUA_______C___UUUU_____GCUUC____A_________UAAUA____U_____AAAGA...AUA-3'\n______________GCUUUUU_UUA____GGACA_______UG_UCCGUGGUA_____CAUG_UAGUG\n______________:||:||:_|:|____|||||_______||_||||::|:|_____|||:_||:||\n______________UGAGAAG_AGU____CCUGU_______AC_AGGCGUCGU_____GUAU_AUUAC\n3'-AUU...AAAAA_______U___UGU______AACUGUA____________UCAAG__________AACAG...CAA-5'\n_____________|______________________________________________________|\n_____________179____________________________________________________117\n |
167 -- 173 |
133 -- 139 |
-31.618 kcal/mol |
11.7219 kcal/mol |
10.6253 kcal/mol |
137 190@LB@ | |@LB@5'-AGA...CUUUA C UUUU GCUUC A UAAUA U AAAGA...AUA-3'@LB@ GCUUUUU UUA GGACA UG UCCGUGGUA CAUG UAGUG@LB@ :||:||: |:| ||||| || ||||::|:| |||: ||:||@LB@ UGAGAAG AGU CCUGU AC AGGCGUCGU GUAU AUUAC@LB@3'-AUU...AAAAA U UGU AACUGUA UCAAG AACAG...CAA-5'@LB@ | |@LB@ 179 117@LB@@LB@@LB@Energy -9.42 kcal/mol@LB@Hybridization Energy -31.618 kcal/mol@LB@Unfolding Energy - mRNA 11.7219 kcal/mol@LB@Unfolding Energy - sRNA 10.6253 kcal/mol@LB@@LB@Position - mRNA 138 -- 189@LB@Position - sRNA 118 -- 178@LB@Position Seed - mRNA 167 -- 173@LB@Position Seed - sRNA 133 -- 139@LB@ |
