| 61 |
0.001915 |
0.1214 |
bsu19720 |
yodR |
-8.11 |
0.600668 |
13 -- 29 |
71 -- 89 |
coenzyme A transferase subunit beta |
|
_____________12___________30\n_____________|____________|\n5'-GCU...GAAGA____A_______ACCGG...CCU-3'\n______________AAUG_UUGUCAC\n______________||||_|||||||\n______________UUAC_AACAGUG\n3'-AUU...GUAUA____________AAAGA...CAA-5'\n_____________|____________|\n_____________90___________70\n |
143 -- 149 |
111 -- 117 |
-12.213 kcal/mol |
1.50216 kcal/mol |
3.63424 kcal/mol |
12 30@LB@ | |@LB@5'-GCU...GAAGA A ACCGG...CCU-3'@LB@ AAUG UUGUCAC@LB@ |||| |||||||@LB@ UUAC AACAGUG@LB@3'-AUU...GUAUA AAAGA...CAA-5'@LB@ | |@LB@ 90 70@LB@@LB@@LB@Energy -8.11 kcal/mol@LB@Hybridization Energy -12.213 kcal/mol@LB@Unfolding Energy - mRNA 1.50216 kcal/mol@LB@Unfolding Energy - sRNA 3.63424 kcal/mol@LB@@LB@Position - mRNA 13 -- 29@LB@Position - sRNA 71 -- 89@LB@Position Seed - mRNA 143 -- 149@LB@Position Seed - sRNA 111 -- 117@LB@ |
| 62 |
0.001958 |
0.1214 |
bsu25150 |
yqfQ |
-10.78 |
0.228520 |
210 -- 221 |
125 -- 139 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________209_____________222\n_____________|_______________|\n5'-AAU...UUUCA_______________AUGAG...UGC-3'\n______________CCGCAGCAG___CCA\n______________||||||||:___|||\n______________GGCGUCGUU___GGU\n3'-AUU...UAACA_________CAA___AUAUU...CAA-5'\n_____________|_______________|\n_____________140_____________124\n |
212 -- 218 |
131 -- 137 |
-17.5181 kcal/mol |
2.74724 kcal/mol |
6.06036 kcal/mol |
209 222@LB@ | |@LB@5'-AAU...UUUCA AUGAG...UGC-3'@LB@ CCGCAGCAG CCA@LB@ ||||||||: |||@LB@ GGCGUCGUU GGU@LB@3'-AUU...UAACA CAA AUAUU...CAA-5'@LB@ | |@LB@ 140 124@LB@@LB@@LB@Energy -10.78 kcal/mol@LB@Hybridization Energy -17.5181 kcal/mol@LB@Unfolding Energy - mRNA 2.74724 kcal/mol@LB@Unfolding Energy - sRNA 6.06036 kcal/mol@LB@@LB@Position - mRNA 210 -- 221@LB@Position - sRNA 125 -- 139@LB@Position Seed - mRNA 212 -- 218@LB@Position Seed - sRNA 131 -- 137@LB@ |
| 63 |
0.001974 |
0.1214 |
bsu34640 |
yvdD |
-9.92 |
0.323853 |
41 -- 65 |
33 -- 60 |
LOG family protein YvdD |
|
_____________40___________________________66\n_____________|____________________________|\n5'-GAC...GUAAA______UCAUCCAUUUUUCUG_______CAGCU...GAA-3'\n______________ACUCUU_______________CAUCAGC\n______________||||||_______________|||||:|\n______________UGAGAA_______________GUAGUUG\n3'-AUU...AAAAA____________________________UCCUG...CAA-5'\n_____________|____________________________|\n_____________61___________________________32\n |
69 -- 75 |
156 -- 162 |
-11.7297 kcal/mol |
2.56294 kcal/mol |
1.40592 kcal/mol |
40 66@LB@ | |@LB@5'-GAC...GUAAA UCAUCCAUUUUUCUG CAGCU...GAA-3'@LB@ ACUCUU CAUCAGC@LB@ |||||| |||||:|@LB@ UGAGAA GUAGUUG@LB@3'-AUU...AAAAA UCCUG...CAA-5'@LB@ | |@LB@ 61 32@LB@@LB@@LB@Energy -9.92 kcal/mol@LB@Hybridization Energy -11.7297 kcal/mol@LB@Unfolding Energy - mRNA 2.56294 kcal/mol@LB@Unfolding Energy - sRNA 1.40592 kcal/mol@LB@@LB@Position - mRNA 41 -- 65@LB@Position - sRNA 33 -- 60@LB@Position Seed - mRNA 69 -- 75@LB@Position Seed - sRNA 156 -- 162@LB@ |
| 64 |
0.002007 |
0.1215 |
bsu37680 |
ywfH |
-19.51 |
0.003623 |
4 -- 58 |
32 -- 83 |
bacilysin biosynthesis oxidoreductase YwfH |
|
_____________3___________________________________________________________59\n_____________|___________________________________________________________|\n_________5'-AA___G______U____U__C_____________U__G____AUACAC_______A_____AUAUG...AUU-3'\n______________UUU_GUU_CA_UCUU_GU_GGCAAUC__ACCU_UC_CUUG______UUCCUCA_UUCUU\n______________:||_|||_||_||||_|:_::|||||__|||:_||_|||:______||||:||_|||||\n______________GAA_CAA_GU_AGAA_CG_UUGUUAG__UGGG_AG_GAAU______AAGGGGU_AAGAA\n3'-AUU...UUACC___G___C__U____C__U_______CA____U__A_________________A_____ACUUU...CAA-5'\n_____________|___________________________________________________________|\n_____________84__________________________________________________________31\n |
46 -- 52 |
38 -- 44 |
-36.3278 kcal/mol |
5.00359 kcal/mol |
14.596 kcal/mol |
3 59@LB@ | |@LB@ 5'-AA G U U C U G AUACAC A AUAUG...AUU-3'@LB@ UUU GUU CA UCUU GU GGCAAUC ACCU UC CUUG UUCCUCA UUCUU@LB@ :|| ||| || |||| |: ::||||| |||: || |||: ||||:|| |||||@LB@ GAA CAA GU AGAA CG UUGUUAG UGGG AG GAAU AAGGGGU AAGAA@LB@3'-AUU...UUACC G C U C U CA U A A ACUUU...CAA-5'@LB@ | |@LB@ 84 31@LB@@LB@@LB@Energy -19.51 kcal/mol@LB@Hybridization Energy -36.3278 kcal/mol@LB@Unfolding Energy - mRNA 5.00359 kcal/mol@LB@Unfolding Energy - sRNA 14.596 kcal/mol@LB@@LB@Position - mRNA 4 -- 58@LB@Position - sRNA 32 -- 83@LB@Position Seed - mRNA 46 -- 52@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 65 |
0.002089 |
0.123 |
bsu15330 |
sigG |
-7.32 |
0.735765 |
1 -- 8 |
133 -- 140 |
RNA polymerase sigma-G factor |
|
______________1_______9\n______________|_______|\n___________5'-________UUGAG...AUG-3'\n______________UUCGCGGC\n______________|:|||:||\n______________AGGCGUCG\n3'-AUU...GUAAC________UUCAA...CAA-5'\n_____________|________|\n_____________141______132\n |
2 -- 8 |
133 -- 139 |
-12.0754 kcal/mol |
1.63369 kcal/mol |
4.3229 kcal/mol |
1 9@LB@ | |@LB@ 5'- UUGAG...AUG-3'@LB@ UUCGCGGC@LB@ |:|||:||@LB@ AGGCGUCG@LB@3'-AUU...GUAAC UUCAA...CAA-5'@LB@ | |@LB@ 141 132@LB@@LB@@LB@Energy -7.32 kcal/mol@LB@Hybridization Energy -12.0754 kcal/mol@LB@Unfolding Energy - mRNA 1.63369 kcal/mol@LB@Unfolding Energy - sRNA 4.3229 kcal/mol@LB@@LB@Position - mRNA 1 -- 8@LB@Position - sRNA 133 -- 140@LB@Position Seed - mRNA 2 -- 8@LB@Position Seed - sRNA 133 -- 139@LB@ |
| 66 |
0.002095 |
0.123 |
bsu28660 |
sspI |
-12.58 |
0.103691 |
257 -- 278 |
56 -- 77 |
small acid-soluble spore protein I |
|
_____________256_____________________279\n_____________|_______________________|\n5'-UUG...CAGGA_________A___U_________GAUGC...AAG-3'\n______________GCAG_CUUG_GCA_ACAAUCGUA\n______________|||:_||||_|||_|||||||||\n______________CGUU_GAAC_CGU_UGUUAGCAU\n3'-AUU...AGCAA____A________U_________GGGUA...CAA-5'\n_____________|_______________________|\n_____________78______________________55\n |
271 -- 277 |
57 -- 63 |
-25.139 kcal/mol |
6.36558 kcal/mol |
6.79705 kcal/mol |
256 279@LB@ | |@LB@5'-UUG...CAGGA A U GAUGC...AAG-3'@LB@ GCAG CUUG GCA ACAAUCGUA@LB@ |||: |||| ||| |||||||||@LB@ CGUU GAAC CGU UGUUAGCAU@LB@3'-AUU...AGCAA A U GGGUA...CAA-5'@LB@ | |@LB@ 78 55@LB@@LB@@LB@Energy -12.58 kcal/mol@LB@Hybridization Energy -25.139 kcal/mol@LB@Unfolding Energy - mRNA 6.36558 kcal/mol@LB@Unfolding Energy - sRNA 6.79705 kcal/mol@LB@@LB@Position - mRNA 257 -- 278@LB@Position - sRNA 56 -- 77@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 57 -- 63@LB@ |
| 67 |
0.002241 |
0.1242 |
bsu04040 |
ycsE |
-12.95 |
0.087665 |
83 -- 153 |
117 -- 184 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; Product type pe: enzyme' |
|
_____________82________________________________________________________________________________154\n_____________|_________________________________________________________________________________|\n________5'-AUC____C____AAG________C__GG___UUU__U___G_________GC______________GUCAU______UC_____CACCG...GCA-3'\n______________GGAU_GGUU___UCGUUGUG_UC__GGU___CA_UUG__UUUGUUUU__UGUUUUU__UUUCU_____GUUUGA__AC_CU\n______________|:||_||:|___||||||::_||__||:___||_|:|__::::|:|:__|:||:::__|||||_____:|||||__||_||\n______________CUUA_CCGA___AGCAACGU_AG__CCG___GU_AGC__GGGUAGAG__AUAAGGG__AAAGA_____UAAACU__UG_GA\n3'-AUU...CAUUA____________________U__AA___UUU__U___AU________A________GU_____AACUU______UU__C__AUUAA...CAA-5'\n_____________|_________________________________________________________________________________|\n_____________185_______________________________________________________________________________116\n |
52 -- 58 |
40 -- 46 |
-38.9686 kcal/mol |
11.5965 kcal/mol |
18.2899 kcal/mol |
82 154@LB@ | |@LB@ 5'-AUC C AAG C GG UUU U G GC GUCAU UC CACCG...GCA-3'@LB@ GGAU GGUU UCGUUGUG UC GGU CA UUG UUUGUUUU UGUUUUU UUUCU GUUUGA AC CU@LB@ |:|| ||:| ||||||:: || ||: || |:| ::::|:|: |:||::: ||||| :||||| || ||@LB@ CUUA CCGA AGCAACGU AG CCG GU AGC GGGUAGAG AUAAGGG AAAGA UAAACU UG GA@LB@3'-AUU...CAUUA U AA UUU U AU A GU AACUU UU C AUUAA...CAA-5'@LB@ | |@LB@ 185 116@LB@@LB@@LB@Energy -12.95 kcal/mol@LB@Hybridization Energy -38.9686 kcal/mol@LB@Unfolding Energy - mRNA 11.5965 kcal/mol@LB@Unfolding Energy - sRNA 18.2899 kcal/mol@LB@@LB@Position - mRNA 83 -- 153@LB@Position - sRNA 117 -- 184@LB@Position Seed - mRNA 52 -- 58@LB@Position Seed - sRNA 40 -- 46@LB@ |
| 68 |
0.002291 |
0.1242 |
bsu02600 |
cwlJ |
-9.38 |
0.396883 |
135 -- 166 |
88 -- 116 |
cell wall hydrolase CwlJ |
|
_____________134______________________________167\n_____________|________________________________|\n5'-UGU...UCUCC____C__CCG_____AC___U___________AUAUG...UCG-3'\n______________UGUC_CU___UCAUC__UUC_GAAGUAAUGAA\n______________||||_||___|||||__|||_:||||||||||\n______________ACAG_GA___AGUAG__AAG_UUUCAUUACUU\n3'-AUU...UUACA____U__AAG______________________ACCGA...CAA-5'\n_____________|________________________________|\n_____________117______________________________87\n |
159 -- 165 |
89 -- 95 |
-22.7508 kcal/mol |
5.74597 kcal/mol |
10.169 kcal/mol |
134 167@LB@ | |@LB@5'-UGU...UCUCC C CCG AC U AUAUG...UCG-3'@LB@ UGUC CU UCAUC UUC GAAGUAAUGAA@LB@ |||| || ||||| ||| :||||||||||@LB@ ACAG GA AGUAG AAG UUUCAUUACUU@LB@3'-AUU...UUACA U AAG ACCGA...CAA-5'@LB@ | |@LB@ 117 87@LB@@LB@@LB@Energy -9.38 kcal/mol@LB@Hybridization Energy -22.7508 kcal/mol@LB@Unfolding Energy - mRNA 5.74597 kcal/mol@LB@Unfolding Energy - sRNA 10.169 kcal/mol@LB@@LB@Position - mRNA 135 -- 166@LB@Position - sRNA 88 -- 116@LB@Position Seed - mRNA 159 -- 165@LB@Position Seed - sRNA 89 -- 95@LB@ |
| 69 |
0.002314 |
0.1242 |
bsu15650 |
yloB |
-14.32 |
0.046100 |
4 -- 34 |
102 -- 149 |
calcium-transporting ATPase |
|
_____________3________________________________________35\n_____________|________________________________________|\n5'-UUU...AAUUC________________________________________AAGUU...ACA-3'\n______________GUCCGC________CCA__________UUAUUUUCUCGUU\n______________||||||________|||__________|:|:||||||:|:\n______________CAGGCG________GGU__________AGUGAAAGAGUAG\n3'-AUU...UGUAA______UCGUUCAA___AUAUUACAAC_____________AAGUU...CAA-5'\n_____________|________________________________________|\n_____________150______________________________________101\n |
28 -- 34 |
102 -- 108 |
-22.6804 kcal/mol |
0.390781 kcal/mol |
10.7571 kcal/mol |
3 35@LB@ | |@LB@5'-UUU...AAUUC AAGUU...ACA-3'@LB@ GUCCGC CCA UUAUUUUCUCGUU@LB@ |||||| ||| |:|:||||||:|:@LB@ CAGGCG GGU AGUGAAAGAGUAG@LB@3'-AUU...UGUAA UCGUUCAA AUAUUACAAC AAGUU...CAA-5'@LB@ | |@LB@ 150 101@LB@@LB@@LB@Energy -14.32 kcal/mol@LB@Hybridization Energy -22.6804 kcal/mol@LB@Unfolding Energy - mRNA 0.390781 kcal/mol@LB@Unfolding Energy - sRNA 10.7571 kcal/mol@LB@@LB@Position - mRNA 4 -- 34@LB@Position - sRNA 102 -- 149@LB@Position Seed - mRNA 28 -- 34@LB@Position Seed - sRNA 102 -- 108@LB@ |
| 70 |
0.00232 |
0.1242 |
bsu24010 |
bmr |
-11.41 |
0.174600 |
110 -- 147 |
39 -- 86 |
multidrug resistance protein |
bsu26590 |
_____________109_______148\n_____________|_________|\n5'-CAA...ACAUG_________GCAAA...UGA-3'\n______________CUUGUUCCC\n______________|||:|||||\n______________GAAUAAGGG\n3'-AUU...GUAGA_________GUAAA...CAA-5'\n_____________|_________|\n_____________87________38\n |
82 -- 88 |
40 -- 46 |
-12.1575 kcal/mol |
2.82835 kcal/mol |
0.440898 kcal/mol |
109 148@LB@ | |@LB@5'-CAA...ACAUG GCAAA...UGA-3'@LB@ CUUGUUCCC@LB@ |||:|||||@LB@ GAAUAAGGG@LB@3'-AUU...GUAGA GUAAA...CAA-5'@LB@ | |@LB@ 87 38@LB@@LB@@LB@Energy -11.41 kcal/mol@LB@Hybridization Energy -12.1575 kcal/mol@LB@Unfolding Energy - mRNA 2.82835 kcal/mol@LB@Unfolding Energy - sRNA 0.440898 kcal/mol@LB@@LB@Position - mRNA 110 -- 147@LB@Position - sRNA 39 -- 86@LB@Position Seed - mRNA 82 -- 88@LB@Position Seed - sRNA 40 -- 46@LB@ |
