Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
51	0.004574	0.3024	b3529	yhjK	-9.99	0.121635	195 -- 213	1 -- 18	cyclic-di-GMP phosphodiesterase	_____________194_________________214\n_____________\|___________________\|\n5'-AGC...GGGGA_____UU_______AAG__GCUCG...AUC-3'\n______________GACAA__UGCGCGU___UC\n______________:\|\|\|\|__\|\|\|\|\|\|\|___\|\|\n______________UUGUU__ACGCGCA___AG\n3'-UUU...UACUA_____U________GAA__-5'\n_____________\|__________________\|\n_____________19_________________1\n	202 -- 208	6 -- 12	-16.8324 kcal/mol	5.6017 kcal/mol	1.24077 kcal/mol	194 214@LB@ \| \|@LB@5'-AGC...GGGGA UU AAG GCUCG...AUC-3'@LB@ GACAA UGCGCGU UC@LB@ :\|\|\|\| \|\|\|\|\|\|\| \|\|@LB@ UUGUU ACGCGCA AG@LB@3'-UUU...UACUA U GAA -5'@LB@ \| \|@LB@ 19 1@LB@@LB@@LB@Energy -9.99 kcal/mol@LB@Hybridization Energy -16.8324 kcal/mol@LB@Unfolding Energy - mRNA 5.6017 kcal/mol@LB@Unfolding Energy - sRNA 1.24077 kcal/mol@LB@@LB@Position - mRNA 195 -- 213@LB@Position - sRNA 1 -- 18@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 6 -- 12@LB@
52	0.004683	0.3024	b1130	phoP	-12.79	0.039529	186 -- 208	6 -- 31	response regulator in two-component regulatory system with PhoQ	_____________185________________________209\n_____________\|__________________________\|\n5'-GAG...UAAGA_________A________________ACUGG...CCA-3'\n______________CAGGGA_GA__AUAA_AAAUGCGCGU\n______________\|\|\|\|\|\|_\|\|__\|\|\|\|_\|\|\|\|\|\|\|\|\|\|\n______________GUCCCU_CU__UAUU_UUUACGCGCA\n3'-UUU...CUUAA______A__AC____G__________GAAAG-5'\n_____________\|__________________________\|\n_____________32_________________________5\n	202 -- 208	6 -- 12	-26.853 kcal/mol	6.30535 kcal/mol	7.75407 kcal/mol	185 209@LB@ \| \|@LB@5'-GAG...UAAGA A ACUGG...CCA-3'@LB@ CAGGGA GA AUAA AAAUGCGCGU@LB@ \|\|\|\|\|\| \|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\|@LB@ GUCCCU CU UAUU UUUACGCGCA@LB@3'-UUU...CUUAA A AC G GAAAG-5'@LB@ \| \|@LB@ 32 5@LB@@LB@@LB@Energy -12.79 kcal/mol@LB@Hybridization Energy -26.853 kcal/mol@LB@Unfolding Energy - mRNA 6.30535 kcal/mol@LB@Unfolding Energy - sRNA 7.75407 kcal/mol@LB@@LB@Position - mRNA 186 -- 208@LB@Position - sRNA 6 -- 31@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 6 -- 12@LB@
53	0.004896	0.3024	b3790	wecD	-9.15	0.167607	125 -- 139	7 -- 21	TDP-fucosamine acetyltransferase	_____________124_____________140\n_____________\|_______________\|\n5'-CUG...GGCUC_________G_____AAGCG...UGA-3'\n______________GAUGACGAG_GUGUG\n______________\|\|\|:\|\|:\|:_\|:\|:\|\n______________CUAUUGUUU_CGCGC\n3'-UUU...UACUA_________A_____AGAAAG-5'\n_____________\|_______________\|\n_____________22______________6\n	126 -- 132	14 -- 20	-15.9636 kcal/mol	5.57309 kcal/mol	1.24179 kcal/mol	124 140@LB@ \| \|@LB@5'-CUG...GGCUC G AAGCG...UGA-3'@LB@ GAUGACGAG GUGUG@LB@ \|\|\|:\|\|:\|: \|:\|:\|@LB@ CUAUUGUUU CGCGC@LB@3'-UUU...UACUA A AGAAAG-5'@LB@ \| \|@LB@ 22 6@LB@@LB@@LB@Energy -9.15 kcal/mol@LB@Hybridization Energy -15.9636 kcal/mol@LB@Unfolding Energy - mRNA 5.57309 kcal/mol@LB@Unfolding Energy - sRNA 1.24179 kcal/mol@LB@@LB@Position - mRNA 125 -- 139@LB@Position - sRNA 7 -- 21@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 14 -- 20@LB@
54	0.004897	0.3024	b1949	fliQ	-8.93	0.181731	208 -- 239	7 -- 38	flagellar biosynthesis protein	_____________207_______________________________240\n_____________\|_________________________________\|\n5'-UUC...GACAC__________UC__________G__C__AA___AUGAA...UUA-3'\n______________CUGAA_UCGG__AUGAUGAUGG_GA_UG__GCG\n______________\|\|\|\|\|_\|\|:\|__\|\|\|\|\|\|\|\|::_:\|_\|\|__\|\|\|\n______________GACUU_AGUC__UACUACUAUU_UU_AC__CGC\n3'-UUU...GUAGA_____A____CC__________G__U__G____AGAAAG-5'\n_____________\|_________________________________\|\n_____________39________________________________6\n	222 -- 228	17 -- 23	-27.1148 kcal/mol	8.82457 kcal/mol	9.35859 kcal/mol	207 240@LB@ \| \|@LB@5'-UUC...GACAC UC G C AA AUGAA...UUA-3'@LB@ CUGAA UCGG AUGAUGAUGG GA UG GCG@LB@ \|\|\|\|\| \|\|:\| \|\|\|\|\|\|\|\|:: :\| \|\| \|\|\|@LB@ GACUU AGUC UACUACUAUU UU AC CGC@LB@3'-UUU...GUAGA A CC G U G AGAAAG-5'@LB@ \| \|@LB@ 39 6@LB@@LB@@LB@Energy -8.93 kcal/mol@LB@Hybridization Energy -27.1148 kcal/mol@LB@Unfolding Energy - mRNA 8.82457 kcal/mol@LB@Unfolding Energy - sRNA 9.35859 kcal/mol@LB@@LB@Position - mRNA 208 -- 239@LB@Position - sRNA 7 -- 38@LB@Position Seed - mRNA 222 -- 228@LB@Position Seed - sRNA 17 -- 23@LB@
55	0.005043	0.3024	b1625	cnu	-10.91	0.084723	38 -- 57	1 -- 21	nucleoid-associated oriC-binding protein; H-NS and StpA stabilizing factor	_____________37____________________58\n_____________\|_____________________\|\n5'-UUU...UCAGG_________________AC__UUUUC...AUC-3'\n______________GAUAGUAAA_GCGCGUU__UC\n______________\|\|\|\|::\|\|\|_\|\|\|\|\|\|:__\|\|\n______________CUAUUGUUU_CGCGCAG__AG\n3'-UUU...UACUA_________A_______AA__-5'\n_____________\|____________________\|\n_____________22___________________1\n	47 -- 53	5 -- 11	-17.9355 kcal/mol	5.76636 kcal/mol	1.25763 kcal/mol	37 58@LB@ \| \|@LB@5'-UUU...UCAGG AC UUUUC...AUC-3'@LB@ GAUAGUAAA GCGCGUU UC@LB@ \|\|\|\|::\|\|\| \|\|\|\|\|\|: \|\|@LB@ CUAUUGUUU CGCGCAG AG@LB@3'-UUU...UACUA A AA -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -10.91 kcal/mol@LB@Hybridization Energy -17.9355 kcal/mol@LB@Unfolding Energy - mRNA 5.76636 kcal/mol@LB@Unfolding Energy - sRNA 1.25763 kcal/mol@LB@@LB@Position - mRNA 38 -- 57@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 47 -- 53@LB@Position Seed - sRNA 5 -- 11@LB@
56	0.005155	0.3024	b3091	uxaA	-13.05	0.035558	147 -- 159	6 -- 19	altronate hydrolase	_____________146____________160\n_____________\|______________\|\n5'-CUG...UGCUU______________GACUA...CGC-3'\n______________UAAC_AAUGCGCGU\n______________\|\|\|\|_\|\|\|\|\|\|\|\|\|\n______________AUUG_UUACGCGCA\n3'-UUU...CUACU____U_________GAAAG-5'\n_____________\|______________\|\n_____________20_____________5\n	153 -- 159	6 -- 12	-17.3524 kcal/mol	3.0727 kcal/mol	1.22941 kcal/mol	146 160@LB@ \| \|@LB@5'-CUG...UGCUU GACUA...CGC-3'@LB@ UAAC AAUGCGCGU@LB@ \|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ AUUG UUACGCGCA@LB@3'-UUU...CUACU U GAAAG-5'@LB@ \| \|@LB@ 20 5@LB@@LB@@LB@Energy -13.05 kcal/mol@LB@Hybridization Energy -17.3524 kcal/mol@LB@Unfolding Energy - mRNA 3.0727 kcal/mol@LB@Unfolding Energy - sRNA 1.22941 kcal/mol@LB@@LB@Position - mRNA 147 -- 159@LB@Position - sRNA 6 -- 19@LB@Position Seed - mRNA 153 -- 159@LB@Position Seed - sRNA 6 -- 12@LB@
57	0.005155	0.3024	b3091	uxaA	-13.05	0.035558	147 -- 159	6 -- 19	altronate hydrolase	_____________146____________160\n_____________\|______________\|\n5'-CUG...UGCUU______________GACUA...CGC-3'\n______________UAAC_AAUGCGCGU\n______________\|\|\|\|_\|\|\|\|\|\|\|\|\|\n______________AUUG_UUACGCGCA\n3'-UUU...CUACU____U_________GAAAG-5'\n_____________\|______________\|\n_____________20_____________5\n	153 -- 159	6 -- 12	-17.3524 kcal/mol	3.0727 kcal/mol	1.22941 kcal/mol	146 160@LB@ \| \|@LB@5'-CUG...UGCUU GACUA...CGC-3'@LB@ UAAC AAUGCGCGU@LB@ \|\|\|\| \|\|\|\|\|\|\|\|\|@LB@ AUUG UUACGCGCA@LB@3'-UUU...CUACU U GAAAG-5'@LB@ \| \|@LB@ 20 5@LB@@LB@@LB@Energy -13.05 kcal/mol@LB@Hybridization Energy -17.3524 kcal/mol@LB@Unfolding Energy - mRNA 3.0727 kcal/mol@LB@Unfolding Energy - sRNA 1.22941 kcal/mol@LB@@LB@Position - mRNA 147 -- 159@LB@Position - sRNA 6 -- 19@LB@Position Seed - mRNA 153 -- 159@LB@Position Seed - sRNA 6 -- 12@LB@
58	0.00518	0.3024	b4068	yjcH	-12.19	0.050580	228 -- 240	8 -- 21	DUF485 family inner membrane protein	_____________227____________241\n_____________\|______________\|\n5'-ACC...GGAUA__A___________AUUUC...UGC-3'\n______________GA_GAC_AAUGCGC\n______________\|\|_:\|\|_\|\|\|\|\|\|\|\n______________CU_UUG_UUACGCG\n3'-UUU...UACUA__A___U_______CAGAAAG-5'\n_____________\|______________\|\n_____________22_____________7\n	234 -- 240	8 -- 14	-14.481 kcal/mol	1.05266 kcal/mol	1.23638 kcal/mol	227 241@LB@ \| \|@LB@5'-ACC...GGAUA A AUUUC...UGC-3'@LB@ GA GAC AAUGCGC@LB@ \|\| :\|\| \|\|\|\|\|\|\|@LB@ CU UUG UUACGCG@LB@3'-UUU...UACUA A U CAGAAAG-5'@LB@ \| \|@LB@ 22 7@LB@@LB@@LB@Energy -12.19 kcal/mol@LB@Hybridization Energy -14.481 kcal/mol@LB@Unfolding Energy - mRNA 1.05266 kcal/mol@LB@Unfolding Energy - sRNA 1.23638 kcal/mol@LB@@LB@Position - mRNA 228 -- 240@LB@Position - sRNA 8 -- 21@LB@Position Seed - mRNA 234 -- 240@LB@Position Seed - sRNA 8 -- 14@LB@
59	0.005538	0.3084	b2812	tcdA	-16.71	0.007603	29 -- 48	1 -- 19	tRNA threonylcarbamoyladenosine dehydratase; sulfur acceptor for CsdA	_____________28___________________49\n_____________\|____________________\|\n5'-GUG...AUUAU____U___________G___AUGCC...UUU-3'\n______________UGAC_AAGUGCGCGUU_UUC\n______________\|:\|\|_\|\|:\|\|\|\|\|\|\|:_\|\|\|\n______________AUUG_UUUACGCGCAG_AAG\n3'-UUU...CUACU________________A___-5'\n_____________\|___________________\|\n_____________20__________________1\n	38 -- 44	5 -- 11	-22.1898 kcal/mol	4.2278 kcal/mol	1.25156 kcal/mol	28 49@LB@ \| \|@LB@5'-GUG...AUUAU U G AUGCC...UUU-3'@LB@ UGAC AAGUGCGCGUU UUC@LB@ \|:\|\| \|\|:\|\|\|\|\|\|\|: \|\|\|@LB@ AUUG UUUACGCGCAG AAG@LB@3'-UUU...CUACU A -5'@LB@ \| \|@LB@ 20 1@LB@@LB@@LB@Energy -16.71 kcal/mol@LB@Hybridization Energy -22.1898 kcal/mol@LB@Unfolding Energy - mRNA 4.2278 kcal/mol@LB@Unfolding Energy - sRNA 1.25156 kcal/mol@LB@@LB@Position - mRNA 29 -- 48@LB@Position - sRNA 1 -- 19@LB@Position Seed - mRNA 38 -- 44@LB@Position Seed - sRNA 5 -- 11@LB@
60	0.00555	0.3084	b2062	wza	-10.85	0.086669	18 -- 25	4 -- 11	colanic acid export protein; outer membrane auxillary lipoprotein	_____________17_______26\n_____________\|________\|\n5'-AAA...CGGUA________GGAUG...GCA-3'\n______________GCGUGUCU\n______________\|\|\|:\|\|\|\|\n______________CGCGCAGA\n3'-UUU...GUUUA________AAG-5'\n_____________\|________\|\n_____________12_______3\n	19 -- 25	4 -- 10	-13.9362 kcal/mol	1.92535 kcal/mol	1.15639 kcal/mol	17 26@LB@ \| \|@LB@5'-AAA...CGGUA GGAUG...GCA-3'@LB@ GCGUGUCU@LB@ \|\|\|:\|\|\|\|@LB@ CGCGCAGA@LB@3'-UUU...GUUUA AAG-5'@LB@ \| \|@LB@ 12 3@LB@@LB@@LB@Energy -10.85 kcal/mol@LB@Hybridization Energy -13.9362 kcal/mol@LB@Unfolding Energy - mRNA 1.92535 kcal/mol@LB@Unfolding Energy - sRNA 1.15639 kcal/mol@LB@@LB@Position - mRNA 18 -- 25@LB@Position - sRNA 4 -- 11@LB@Position Seed - mRNA 19 -- 25@LB@Position Seed - sRNA 4 -- 10@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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