Input and runtime details for job 5344133 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 5344133 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:28:43 CET 2018
	CopraRNA Started		@ Fri Feb 16 20:36:11 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:15:51 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:15:58 CET 2018
	Job Completed		@ Sat Feb 17 02:16:29 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=5344133&toolName=CopraRNA ( 30 days expiry )

Description of the job

RybB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
41	0.002071	0.1648	b0992	yccM	-9.88	0.287125	161 -- 167	1 -- 7	putative 4Fe-4S membrane protein		_____________160_____168\n_____________\|_______\|\n5'-AUC...UAGAA_______AUUUG...AAG-3'\n______________UAGUGGC\n______________:\|\|\|\|\|\|\n______________GUCACCG\n3'-UUU...UUUUC_______-5'\n_____________\|______\|\n_____________8______1\n	161 -- 167	1 -- 7	-11.7646 kcal/mol	0.660682 kcal/mol	1.22779 kcal/mol	160 168@LB@ \| \|@LB@5'-AUC...UAGAA AUUUG...AAG-3'@LB@ UAGUGGC@LB@ :\|\|\|\|\|\|@LB@ GUCACCG@LB@3'-UUU...UUUUC -5'@LB@ \| \|@LB@ 8 1@LB@@LB@@LB@Energy -9.88 kcal/mol@LB@Hybridization Energy -11.7646 kcal/mol@LB@Unfolding Energy - mRNA 0.660682 kcal/mol@LB@Unfolding Energy - sRNA 1.22779 kcal/mol@LB@@LB@Position - mRNA 161 -- 167@LB@Position - sRNA 1 -- 7@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 1 -- 7@LB@
42	0.002133	0.1648	b0968	yccX	-12.44	0.101807	184 -- 198	5 -- 23	weak acylphosphatase		_____________183_________________199\n_____________\|___________________\|\n5'-GGC...AUUAA___________________CAAUG...CCG-3'\n______________GGAUA____AGGAGAGCAG\n______________\|\|\|:\|____\|\|:\|:\|\|\|\|\|\n______________CCUGU____UCUUUUCGUC\n3'-UUU...UUACC_____AGUU__________ACCG-5'\n_____________\|___________________\|\n_____________24__________________4\n	192 -- 198	5 -- 11	-17.1825 kcal/mol	3.17176 kcal/mol	1.57523 kcal/mol	183 199@LB@ \| \|@LB@5'-GGC...AUUAA CAAUG...CCG-3'@LB@ GGAUA AGGAGAGCAG@LB@ \|\|\|:\| \|\|:\|:\|\|\|\|\|@LB@ CCUGU UCUUUUCGUC@LB@3'-UUU...UUACC AGUU ACCG-5'@LB@ \| \|@LB@ 24 4@LB@@LB@@LB@Energy -12.44 kcal/mol@LB@Hybridization Energy -17.1825 kcal/mol@LB@Unfolding Energy - mRNA 3.17176 kcal/mol@LB@Unfolding Energy - sRNA 1.57523 kcal/mol@LB@@LB@Position - mRNA 184 -- 198@LB@Position - sRNA 5 -- 23@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 5 -- 11@LB@
43	0.00215	0.1648	b4268	idnK	-13.73	0.054596	180 -- 268	1 -- 79	D-gluconate kinase thermosensitive	b3437	_____________179______________________________________________________________________________________________269\n_____________\|________________________________________________________________________________________________\|\n5'-AGG...AGGUA___U______GGA______________________U_AAAGCUUUAUU_________GU____G_______UAAA_______UUGGU____A____UGCCG...AUG-3'\n______________AGA_GAUUCA___GAUUUU______AAAUGGCGGG_G___________UUGAUGGGC__UUCA_GGAGUGG____ACAUUAA_____AGCA__GGU\n______________\|:\|_:\|::\|\|___:\|::\|\|______\|\|\|\|\|\|\|\|\|\|_\|___________\|\|\|\|\|\|\|\|\|__\|:\|\|_::\|:\|\|\|____\|\|\|\|:\|\|_____\|\|\|\|__\|\|:\n______________UUU_UUGGGU___UUGGAA______UUUACCGCCC_C___________AACUACCCG__AGGU_UUUUACC____UGUAGUU_____UCGU__CCG\n___________3'-___U______AG_______CUUGGC______________________________________G_______CC_________UCUUU____CA___-5'\n______________\|______________________________________________________________________________________________\|\n______________79_____________________________________________________________________________________________1\n	249 -- 255	15 -- 21	-57.0668 kcal/mol	16.9855 kcal/mol	26.3515 kcal/mol	179 269@LB@ \| \|@LB@5'-AGG...AGGUA U GGA U AAAGCUUUAUU GU G UAAA UUGGU A UGCCG...AUG-3'@LB@ AGA GAUUCA GAUUUU AAAUGGCGGG G UUGAUGGGC UUCA GGAGUGG ACAUUAA AGCA GGU@LB@ \|:\| :\|::\|\| :\|::\|\| \|\|\|\|\|\|\|\|\|\| \| \|\|\|\|\|\|\|\|\| \|:\|\| ::\|:\|\|\| \|\|\|\|:\|\| \|\|\|\| \|\|:@LB@ UUU UUGGGU UUGGAA UUUACCGCCC C AACUACCCG AGGU UUUUACC UGUAGUU UCGU CCG@LB@ 3'- U AG CUUGGC G CC UCUUU CA -5'@LB@ \| \|@LB@ 79 1@LB@@LB@@LB@Energy -13.73 kcal/mol@LB@Hybridization Energy -57.0668 kcal/mol@LB@Unfolding Energy - mRNA 16.9855 kcal/mol@LB@Unfolding Energy - sRNA 26.3515 kcal/mol@LB@@LB@Position - mRNA 180 -- 268@LB@Position - sRNA 1 -- 79@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 15 -- 21@LB@
44	0.002166	0.1648	b3349	slyD	-19.18	0.001707	20 -- 41	5 -- 23	FKBP-type peptidyl prolyl cis-trans isomerase (rotamase)		_____________19_____________________42\n_____________\|______________________\|\n5'-UGU...AGUGC__________UGC_________ACAAA...ACU-3'\n______________GGACAUCAGA___GAGAAGCAG\n______________\|\|\|\|\|\|\|\|:\|___\|\|:\|\|\|\|\|\|\n______________CCUGUAGUUU___CUUUUCGUC\n3'-UUU...UUACC______________________ACCG-5'\n_____________\|______________________\|\n_____________24_____________________4\n	35 -- 41	5 -- 11	-25.7728 kcal/mol	5.01574 kcal/mol	1.57523 kcal/mol	19 42@LB@ \| \|@LB@5'-UGU...AGUGC UGC ACAAA...ACU-3'@LB@ GGACAUCAGA GAGAAGCAG@LB@ \|\|\|\|\|\|\|\|:\| \|\|:\|\|\|\|\|\|@LB@ CCUGUAGUUU CUUUUCGUC@LB@3'-UUU...UUACC ACCG-5'@LB@ \| \|@LB@ 24 4@LB@@LB@@LB@Energy -19.18 kcal/mol@LB@Hybridization Energy -25.7728 kcal/mol@LB@Unfolding Energy - mRNA 5.01574 kcal/mol@LB@Unfolding Energy - sRNA 1.57523 kcal/mol@LB@@LB@Position - mRNA 20 -- 41@LB@Position - sRNA 5 -- 23@LB@Position Seed - mRNA 35 -- 41@LB@Position Seed - sRNA 5 -- 11@LB@
45	0.002187	0.1648	b3391	hofQ	-14.99	0.027914	2 -- 20	1 -- 20	DNA catabolic putative fimbrial transporter		_____________1____________________21\n_____________\|____________________\|\n__________5'-C__________U_________GAGGC...UAG-3'\n______________UAUCAGGGGA_GGUAG_GGC\n______________:\|\|\|\|::\|:\|_:\|:\|\|_\|\|\|\n______________GUAGUUUCUU_UCGUC_CCG\n3'-UUU...CCCCU__________U_____A___-5'\n_____________\|___________________\|\n_____________21__________________1\n	5 -- 11	11 -- 17	-22.4478 kcal/mol	5.99969 kcal/mol	1.45855 kcal/mol	1 21@LB@ \| \|@LB@ 5'-C U GAGGC...UAG-3'@LB@ UAUCAGGGGA GGUAG GGC@LB@ :\|\|\|\|::\|:\| :\|:\|\| \|\|\|@LB@ GUAGUUUCUU UCGUC CCG@LB@3'-UUU...CCCCU U A -5'@LB@ \| \|@LB@ 21 1@LB@@LB@@LB@Energy -14.99 kcal/mol@LB@Hybridization Energy -22.4478 kcal/mol@LB@Unfolding Energy - mRNA 5.99969 kcal/mol@LB@Unfolding Energy - sRNA 1.45855 kcal/mol@LB@@LB@Position - mRNA 2 -- 20@LB@Position - sRNA 1 -- 20@LB@Position Seed - mRNA 5 -- 11@LB@Position Seed - sRNA 11 -- 17@LB@
46	0.002205	0.1648	b1662	ribC	-17.71	0.005126	34 -- 61	2 -- 29	riboflavin synthase alpha subunit		_____________33_____________________________62\n_____________\|______________________________\|\n5'-UUU...CGCCU_______CA___A_____A___________GAAAU...UGG-3'\n______________GAAUGGG__GCA__AAAG_AGAAGCAG_GG\n______________:\|\|\|\|\|\|__:\|\|__\|\|\|\|_\|:\|\|\|\|\|\|_\|\|\n______________UUUACCC__UGU__UUUC_UUUUCGUC_CC\n3'-UUU...GGUGU_______C____AG_____________A__G-5'\n_____________\|______________________________\|\n_____________30_____________________________1\n	53 -- 59	5 -- 11	-25.8778 kcal/mol	5.65259 kcal/mol	2.51906 kcal/mol	33 62@LB@ \| \|@LB@5'-UUU...CGCCU CA A A GAAAU...UGG-3'@LB@ GAAUGGG GCA AAAG AGAAGCAG GG@LB@ :\|\|\|\|\|\| :\|\| \|\|\|\| \|:\|\|\|\|\|\| \|\|@LB@ UUUACCC UGU UUUC UUUUCGUC CC@LB@3'-UUU...GGUGU C AG A G-5'@LB@ \| \|@LB@ 30 1@LB@@LB@@LB@Energy -17.71 kcal/mol@LB@Hybridization Energy -25.8778 kcal/mol@LB@Unfolding Energy - mRNA 5.65259 kcal/mol@LB@Unfolding Energy - sRNA 2.51906 kcal/mol@LB@@LB@Position - mRNA 34 -- 61@LB@Position - sRNA 2 -- 29@LB@Position Seed - mRNA 53 -- 59@LB@Position Seed - sRNA 5 -- 11@LB@
47	0.002271	0.1648	b3169	nusA	-13.61	0.058009	55 -- 79	4 -- 28	transcription termination/antitermination L factor		_____________54__________________________80\n_____________\|___________________________\|\n5'-UUG...UCGUA_____CA________UC__________AGACG...CAG-3'\n______________AAUGG__GGGCGUUA____AAAGCGGU\n______________\|\|\|\|\|__\|\|:\|:\|:\|____\|\|\|\|\|:\|\|\n______________UUACC__CCUGUAGU____UUUCGUCA\n3'-UUU...GUGUU_______________UUCU________CCG-5'\n_____________\|___________________________\|\n_____________29__________________________3\n	73 -- 79	4 -- 10	-21.8237 kcal/mol	5.69952 kcal/mol	2.51457 kcal/mol	54 80@LB@ \| \|@LB@5'-UUG...UCGUA CA UC AGACG...CAG-3'@LB@ AAUGG GGGCGUUA AAAGCGGU@LB@ \|\|\|\|\| \|\|:\|:\|:\| \|\|\|\|\|:\|\|@LB@ UUACC CCUGUAGU UUUCGUCA@LB@3'-UUU...GUGUU UUCU CCG-5'@LB@ \| \|@LB@ 29 3@LB@@LB@@LB@Energy -13.61 kcal/mol@LB@Hybridization Energy -21.8237 kcal/mol@LB@Unfolding Energy - mRNA 5.69952 kcal/mol@LB@Unfolding Energy - sRNA 2.51457 kcal/mol@LB@@LB@Position - mRNA 55 -- 79@LB@Position - sRNA 4 -- 28@LB@Position Seed - mRNA 73 -- 79@LB@Position Seed - sRNA 4 -- 10@LB@
48	0.002302	0.1648	b3460	livJ	-15.11	0.026091	183 -- 229	1 -- 30	branched-chain amino acid ABC transporter periplasmic binding protein		_____________182_____________________________________________230\n_____________\|_______________________________________________\|\n5'-AAU...CCAGA___________UC___GGAUGAACAUAA____U______UUAC____AGGAU...CAA-3'\n______________AGAAUGGGGAU__UCA____________AGGG_AAAGCG____UGGC\n______________\|:\|\|\|\|\|\|\|\|:__\|\|\|____________\|:\|:_\|\|\|\|\|:____\|\|\|\|\n______________UUUUACCCCUG__AGU____________UUCU_UUUCGU____ACCG\n3'-UUU...AGGUG___________U___________________________C_______-5'\n_____________\|______________________________________________\|\n_____________31_____________________________________________1\n	187 -- 193	20 -- 26	-27.0033 kcal/mol	9.37443 kcal/mol	2.51911 kcal/mol	182 230@LB@ \| \|@LB@5'-AAU...CCAGA UC GGAUGAACAUAA U UUAC AGGAU...CAA-3'@LB@ AGAAUGGGGAU UCA AGGG AAAGCG UGGC@LB@ \|:\|\|\|\|\|\|\|\|: \|\|\| \|:\|: \|\|\|\|\|: \|\|\|\|@LB@ UUUUACCCCUG AGU UUCU UUUCGU ACCG@LB@3'-UUU...AGGUG U C -5'@LB@ \| \|@LB@ 31 1@LB@@LB@@LB@Energy -15.11 kcal/mol@LB@Hybridization Energy -27.0033 kcal/mol@LB@Unfolding Energy - mRNA 9.37443 kcal/mol@LB@Unfolding Energy - sRNA 2.51911 kcal/mol@LB@@LB@Position - mRNA 183 -- 229@LB@Position - sRNA 1 -- 30@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 20 -- 26@LB@
49	0.002324	0.1648	b1119	nagK	-12.21	0.112661	180 -- 199	1 -- 21	N-acetyl-D-glucosamine kinase		_____________179___________________200\n_____________\|_____________________\|\n5'-UCA...GCAGU________AC____G__C___AAUGU...GUG-3'\n______________ACAUUAAA___AAG_AG_GGC\n______________\|\|\|\|:\|\|\|___\|\|\|_\|\|_\|\|\|\n______________UGUAGUUU___UUC_UC_CCG\n3'-UUU...ACCCC________CUU___G__A___-5'\n_____________\|____________________\|\n_____________22___________________1\n	181 -- 187	14 -- 20	-16.408 kcal/mol	2.6857 kcal/mol	1.51103 kcal/mol	179 200@LB@ \| \|@LB@5'-UCA...GCAGU AC G C AAUGU...GUG-3'@LB@ ACAUUAAA AAG AG GGC@LB@ \|\|\|\|:\|\|\| \|\|\| \|\| \|\|\|@LB@ UGUAGUUU UUC UC CCG@LB@3'-UUU...ACCCC CUU G A -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -12.21 kcal/mol@LB@Hybridization Energy -16.408 kcal/mol@LB@Unfolding Energy - mRNA 2.6857 kcal/mol@LB@Unfolding Energy - sRNA 1.51103 kcal/mol@LB@@LB@Position - mRNA 180 -- 199@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 181 -- 187@LB@Position Seed - sRNA 14 -- 20@LB@
50	0.002349	0.1648	b2040	rfbD	-20.63	0.000491	127 -- 157	3 -- 32	dTDP-L-rhamnose synthase NAD(P)-dependent dTDP-4-dehydrorhamnose reductase subunit		_____________126______________________________158\n_____________\|________________________________\|\n5'-GAU...CCAAU__________UU____AUGU____________CCUAU...CUA-3'\n______________ACAAAAUGGG__GAUA_____GAAAAGUGGUG\n______________\|\|\|\|\|\|\|\|\|\|__\|\|:\|_____\|\|\|\|\|\|::\|\|\|\n______________UGUUUUACCC__CUGU_____CUUUUCGUCAC\n3'-UUU...CGAGG________________AGUUU___________CG-5'\n_____________\|________________________________\|\n_____________33_______________________________2\n	151 -- 157	3 -- 9	-27.2371 kcal/mol	4.0691 kcal/mol	2.53925 kcal/mol	126 158@LB@ \| \|@LB@5'-GAU...CCAAU UU AUGU CCUAU...CUA-3'@LB@ ACAAAAUGGG GAUA GAAAAGUGGUG@LB@ \|\|\|\|\|\|\|\|\|\| \|\|:\| \|\|\|\|\|\|::\|\|\|@LB@ UGUUUUACCC CUGU CUUUUCGUCAC@LB@3'-UUU...CGAGG AGUUU CG-5'@LB@ \| \|@LB@ 33 2@LB@@LB@@LB@Energy -20.63 kcal/mol@LB@Hybridization Energy -27.2371 kcal/mol@LB@Unfolding Energy - mRNA 4.0691 kcal/mol@LB@Unfolding Energy - sRNA 2.53925 kcal/mol@LB@@LB@Position - mRNA 127 -- 157@LB@Position - sRNA 3 -- 32@LB@Position Seed - mRNA 151 -- 157@LB@Position Seed - sRNA 3 -- 9@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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