Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
41	0.00276	0.2279	b0889	lrp	-10.49	0.100208	207 -- 221	8 -- 21	leucine-responsive global transcriptional regulator	_____________206_____________222\n_____________\|_______________\|\n5'-GUG...UGGUA_________AA____CCUGG...AGC-3'\n______________GAUAGCAAG__GCGC\n______________\|\|\|\|:\|\|\|:__\|\|\|\|\n______________CUAUUGUUU__CGCG\n3'-UUU...UACUA_________A_____CAGAAAG-5'\n_____________\|_______________\|\n_____________22______________7\n	209 -- 215	13 -- 19	-15.2704 kcal/mol	3.5472 kcal/mol	1.23638 kcal/mol	206 222@LB@ \| \|@LB@5'-GUG...UGGUA AA CCUGG...AGC-3'@LB@ GAUAGCAAG GCGC@LB@ \|\|\|\|:\|\|\|: \|\|\|\|@LB@ CUAUUGUUU CGCG@LB@3'-UUU...UACUA A CAGAAAG-5'@LB@ \| \|@LB@ 22 7@LB@@LB@@LB@Energy -10.49 kcal/mol@LB@Hybridization Energy -15.2704 kcal/mol@LB@Unfolding Energy - mRNA 3.5472 kcal/mol@LB@Unfolding Energy - sRNA 1.23638 kcal/mol@LB@@LB@Position - mRNA 207 -- 221@LB@Position - sRNA 8 -- 21@LB@Position Seed - mRNA 209 -- 215@LB@Position Seed - sRNA 13 -- 19@LB@
42	0.002847	0.2296	b1638	pdxH	-9.07	0.172497	29 -- 35	5 -- 11	pyridoxine 5'-phosphate oxidase	_____________28______36\n_____________\|_______\|\n5'-UCU...CUGGC_______ACACU...CCC-3'\n______________GCGCGUU\n______________\|\|\|\|\|\|:\n______________CGCGCAG\n3'-UUU...GUUUA_______AAAG-5'\n_____________\|_______\|\n_____________12______4\n	29 -- 35	5 -- 11	-12.2635 kcal/mol	2.08033 kcal/mol	1.11145 kcal/mol	28 36@LB@ \| \|@LB@5'-UCU...CUGGC ACACU...CCC-3'@LB@ GCGCGUU@LB@ \|\|\|\|\|\|:@LB@ CGCGCAG@LB@3'-UUU...GUUUA AAAG-5'@LB@ \| \|@LB@ 12 4@LB@@LB@@LB@Energy -9.07 kcal/mol@LB@Hybridization Energy -12.2635 kcal/mol@LB@Unfolding Energy - mRNA 2.08033 kcal/mol@LB@Unfolding Energy - sRNA 1.11145 kcal/mol@LB@@LB@Position - mRNA 29 -- 35@LB@Position - sRNA 5 -- 11@LB@Position Seed - mRNA 29 -- 35@LB@Position Seed - sRNA 5 -- 11@LB@
43	0.003009	0.2325	b3429	glgA	-8.59	0.206361	152 -- 163	7 -- 22	glycogen synthase	_____________151______________164\n_____________\|________________\|\n5'-GUA...CGUGC________________AAAUG...ACG-3'\n______________UGGUAAC____GCGCG\n______________\|\|:\|\|\|\|____\|\|\|\|\|\n______________ACUAUUG____CGCGC\n3'-UUU...CUACU_______UUUA_____AGAAAG-5'\n_____________\|________________\|\n_____________23_______________6\n	152 -- 158	16 -- 22	-13.429 kcal/mol	3.59934 kcal/mol	1.24379 kcal/mol	151 164@LB@ \| \|@LB@5'-GUA...CGUGC AAAUG...ACG-3'@LB@ UGGUAAC GCGCG@LB@ \|\|:\|\|\|\| \|\|\|\|\|@LB@ ACUAUUG CGCGC@LB@3'-UUU...CUACU UUUA AGAAAG-5'@LB@ \| \|@LB@ 23 6@LB@@LB@@LB@Energy -8.59 kcal/mol@LB@Hybridization Energy -13.429 kcal/mol@LB@Unfolding Energy - mRNA 3.59934 kcal/mol@LB@Unfolding Energy - sRNA 1.24379 kcal/mol@LB@@LB@Position - mRNA 152 -- 163@LB@Position - sRNA 7 -- 22@LB@Position Seed - mRNA 152 -- 158@LB@Position Seed - sRNA 16 -- 22@LB@
44	0.003062	0.2325	b3847	pepQ	-9.98	0.122201	144 -- 157	8 -- 21	proline dipeptidase	_____________143____________158\n_____________\|______________\|\n5'-UUG...CUCUG_____AC_______UAAGA...UUC-3'\n______________GAUGA__GAUGUGC\n______________\|\|\|:\|__:\|\|\|:\|\|\n______________CUAUU__UUACGCG\n3'-UUU...UACUA_____GU_______CAGAAAG-5'\n_____________\|______________\|\n_____________22_____________7\n	151 -- 157	8 -- 14	-13.3112 kcal/mol	2.09686 kcal/mol	1.23638 kcal/mol	143 158@LB@ \| \|@LB@5'-UUG...CUCUG AC UAAGA...UUC-3'@LB@ GAUGA GAUGUGC@LB@ \|\|\|:\| :\|\|\|:\|\|@LB@ CUAUU UUACGCG@LB@3'-UUU...UACUA GU CAGAAAG-5'@LB@ \| \|@LB@ 22 7@LB@@LB@@LB@Energy -9.98 kcal/mol@LB@Hybridization Energy -13.3112 kcal/mol@LB@Unfolding Energy - mRNA 2.09686 kcal/mol@LB@Unfolding Energy - sRNA 1.23638 kcal/mol@LB@@LB@Position - mRNA 144 -- 157@LB@Position - sRNA 8 -- 21@LB@Position Seed - mRNA 151 -- 157@LB@Position Seed - sRNA 8 -- 14@LB@
45	0.00309	0.2325	b0584	fepA	-13.78	0.026215	91 -- 106	1 -- 17	ferrienterobactin outer membrane transporter	_____________90________________107\n_____________\|_________________\|\n5'-GAA...CAACU___C_____________AGGAU...ACG-3'\n______________GCA__UGCGUGUCUUUC\n______________:\|\|__\|\|\|\|:\|\|\|\|\|\|\|\n______________UGU__ACGCGCAGAAAG\n3'-UUU...ACUAU___UU____________-5'\n_____________\|________________\|\n_____________18_______________1\n	100 -- 106	1 -- 7	-21.1338 kcal/mol	6.10916 kcal/mol	1.23994 kcal/mol	90 107@LB@ \| \|@LB@5'-GAA...CAACU C AGGAU...ACG-3'@LB@ GCA UGCGUGUCUUUC@LB@ :\|\| \|\|\|\|:\|\|\|\|\|\|\|@LB@ UGU ACGCGCAGAAAG@LB@3'-UUU...ACUAU UU -5'@LB@ \| \|@LB@ 18 1@LB@@LB@@LB@Energy -13.78 kcal/mol@LB@Hybridization Energy -21.1338 kcal/mol@LB@Unfolding Energy - mRNA 6.10916 kcal/mol@LB@Unfolding Energy - sRNA 1.23994 kcal/mol@LB@@LB@Position - mRNA 91 -- 106@LB@Position - sRNA 1 -- 17@LB@Position Seed - mRNA 100 -- 106@LB@Position Seed - sRNA 1 -- 7@LB@
46	0.003408	0.2484	b2518	ndk	-12.77	0.039848	1 -- 16	7 -- 21	multifunctional nucleoside diphosphate kinase and apyrimidinic endonuclease and 3'-phosphodiesterase	______________1_______________17\n______________\|_______________\|\n___________5'-___________A____GGGCA...AAA-3'\n______________GGUGAUGGAUG_CGUG\n______________\|:\|:\|:::\|\|\|_\|\|:\|\n______________CUAUUGUUUAC_GCGC\n3'-UUU...UACUA________________AGAAAG-5'\n_____________\|________________\|\n_____________22_______________6\n	5 -- 11	11 -- 17	-16.3595 kcal/mol	2.34366 kcal/mol	1.24179 kcal/mol	1 17@LB@ \| \|@LB@ 5'- A GGGCA...AAA-3'@LB@ GGUGAUGGAUG CGUG@LB@ \|:\|:\|:::\|\|\| \|\|:\|@LB@ CUAUUGUUUAC GCGC@LB@3'-UUU...UACUA AGAAAG-5'@LB@ \| \|@LB@ 22 6@LB@@LB@@LB@Energy -12.77 kcal/mol@LB@Hybridization Energy -16.3595 kcal/mol@LB@Unfolding Energy - mRNA 2.34366 kcal/mol@LB@Unfolding Energy - sRNA 1.24179 kcal/mol@LB@@LB@Position - mRNA 1 -- 16@LB@Position - sRNA 7 -- 21@LB@Position Seed - mRNA 5 -- 11@LB@Position Seed - sRNA 11 -- 17@LB@
47	0.003448	0.2484	b0614	citX	-10.13	0.115098	76 -- 91	4 -- 17	apo-citrate lyase phosphoribosyl-dephospho-CoA transferase	_____________75_______________92\n_____________\|________________\|\n5'-UUA...GCUGC____AC__________GCUGA...UCA-3'\n______________GCGA__GUGCGCGUCU\n______________:\|:\|__:\|\|\|\|\|\|\|\|\|\n______________UGUU__UACGCGCAGA\n3'-UUU...ACUAU________________AAG-5'\n_____________\|________________\|\n_____________18_______________3\n	85 -- 91	4 -- 10	-19.1919 kcal/mol	7.83057 kcal/mol	1.22888 kcal/mol	75 92@LB@ \| \|@LB@5'-UUA...GCUGC AC GCUGA...UCA-3'@LB@ GCGA GUGCGCGUCU@LB@ :\|:\| :\|\|\|\|\|\|\|\|\|@LB@ UGUU UACGCGCAGA@LB@3'-UUU...ACUAU AAG-5'@LB@ \| \|@LB@ 18 3@LB@@LB@@LB@Energy -10.13 kcal/mol@LB@Hybridization Energy -19.1919 kcal/mol@LB@Unfolding Energy - mRNA 7.83057 kcal/mol@LB@Unfolding Energy - sRNA 1.22888 kcal/mol@LB@@LB@Position - mRNA 76 -- 91@LB@Position - sRNA 4 -- 17@LB@Position Seed - mRNA 85 -- 91@LB@Position Seed - sRNA 4 -- 10@LB@
48	0.00405	0.2857	b0409	secF	-13.37	0.031213	166 -- 172	5 -- 11	SecYEG protein translocase auxillary subunit	_____________165_____173\n_____________\|_______\|\n5'-CUU...GGCAA_______AAGAA...UGU-3'\n______________GCGCGUC\n______________\|\|\|\|\|\|\|\n______________CGCGCAG\n3'-UUU...GUUUA_______AAAG-5'\n_____________\|_______\|\n_____________12______4\n	166 -- 172	5 -- 11	-14.9857 kcal/mol	0.509172 kcal/mol	1.11145 kcal/mol	165 173@LB@ \| \|@LB@5'-CUU...GGCAA AAGAA...UGU-3'@LB@ GCGCGUC@LB@ \|\|\|\|\|\|\|@LB@ CGCGCAG@LB@3'-UUU...GUUUA AAAG-5'@LB@ \| \|@LB@ 12 4@LB@@LB@@LB@Energy -13.37 kcal/mol@LB@Hybridization Energy -14.9857 kcal/mol@LB@Unfolding Energy - mRNA 0.509172 kcal/mol@LB@Unfolding Energy - sRNA 1.11145 kcal/mol@LB@@LB@Position - mRNA 166 -- 172@LB@Position - sRNA 5 -- 11@LB@Position Seed - mRNA 166 -- 172@LB@Position Seed - sRNA 5 -- 11@LB@
49	0.004401	0.299	b0093	ftsQ	-10.19	0.112476	259 -- 274	4 -- 20	divisome assembly protein membrane anchored protein involved in growth of wall at septum	_____________258_______________275\n_____________\|_________________\|\n5'-GCU...CCGCA________A________GGCGG...CCG-3'\n______________AUAAUGGA__CGCGUCU\n______________\|\|\|\|:::\|__\|\|\|\|\|\|\|\n______________UAUUGUUU__GCGCAGA\n3'-UUU...ACUAC________AC_______AAG-5'\n_____________\|_________________\|\n_____________21________________3\n	268 -- 274	4 -- 10	-13.8485 kcal/mol	2.40331 kcal/mol	1.25368 kcal/mol	258 275@LB@ \| \|@LB@5'-GCU...CCGCA A GGCGG...CCG-3'@LB@ AUAAUGGA CGCGUCU@LB@ \|\|\|\|:::\| \|\|\|\|\|\|\|@LB@ UAUUGUUU GCGCAGA@LB@3'-UUU...ACUAC AC AAG-5'@LB@ \| \|@LB@ 21 3@LB@@LB@@LB@Energy -10.19 kcal/mol@LB@Hybridization Energy -13.8485 kcal/mol@LB@Unfolding Energy - mRNA 2.40331 kcal/mol@LB@Unfolding Energy - sRNA 1.25368 kcal/mol@LB@@LB@Position - mRNA 259 -- 274@LB@Position - sRNA 4 -- 20@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 4 -- 10@LB@
50	0.004415	0.299	b0752	zitB	-14.03	0.023662	243 -- 258	5 -- 21	zinc efflux system	_____________242_______________259\n_____________\|_________________\|\n5'-CGC...CAGAA____________U____GCUUG...UGG-3'\n______________GAUAAUAA_UGC_CGUC\n______________\|\|\|\|\|:\|\|_\|\|\|_\|\|\|\|\n______________CUAUUGUU_ACG_GCAG\n3'-UUU...UACUA________U___C____AAAG-5'\n_____________\|_________________\|\n_____________22________________4\n	244 -- 250	14 -- 20	-17.36 kcal/mol	2.08049 kcal/mol	1.24955 kcal/mol	242 259@LB@ \| \|@LB@5'-CGC...CAGAA U GCUUG...UGG-3'@LB@ GAUAAUAA UGC CGUC@LB@ \|\|\|\|\|:\|\| \|\|\| \|\|\|\|@LB@ CUAUUGUU ACG GCAG@LB@3'-UUU...UACUA U C AAAG-5'@LB@ \| \|@LB@ 22 4@LB@@LB@@LB@Energy -14.03 kcal/mol@LB@Hybridization Energy -17.36 kcal/mol@LB@Unfolding Energy - mRNA 2.08049 kcal/mol@LB@Unfolding Energy - sRNA 1.24955 kcal/mol@LB@@LB@Position - mRNA 243 -- 258@LB@Position - sRNA 5 -- 21@LB@Position Seed - mRNA 244 -- 250@LB@Position Seed - sRNA 14 -- 20@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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