Input and runtime details for job 1786318 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1786318 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:20:27 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:20:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:01:46 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:01:53 CET 2018
	Job Completed		@ Fri Feb 16 22:02:22 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1786318&toolName=CopraRNA ( 30 days expiry )

Description of the job

ArcZ

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
122	0.006658	0.1913	b0600	ybdL	-10.23	0.125775	275 -- 297	68 -- 86	methionine aminotransferase PLP-dependent	_____________274_____________________298\n_____________\|_______________________\|\n5'-UCA...AGCGC___G____AG__________C__UUA-3'\n______________GCU_GCGC__CAACACCAGG_GA\n______________:\|\|_\|\|\|\|__\|\|\|\|\|\|\|\|\|\|_\|\|\n______________UGA_CGCG__GUUGUGGUCC_CU\n3'-UUU...GCUUA_______________________UUAGA...GUG-5'\n_____________\|_______________________\|\n_____________87______________________67\n	288 -- 294	70 -- 76	-27.7616 kcal/mol	10.1544 kcal/mol	7.37523 kcal/mol	274 298@LB@ \| \|@LB@5'-UCA...AGCGC G AG C UUA-3'@LB@ GCU GCGC CAACACCAGG GA@LB@ :\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\| \|\|@LB@ UGA CGCG GUUGUGGUCC CU@LB@3'-UUU...GCUUA UUAGA...GUG-5'@LB@ \| \|@LB@ 87 67@LB@@LB@@LB@Energy -10.23 kcal/mol@LB@Hybridization Energy -27.7616 kcal/mol@LB@Unfolding Energy - mRNA 10.1544 kcal/mol@LB@Unfolding Energy - sRNA 7.37523 kcal/mol@LB@@LB@Position - mRNA 275 -- 297@LB@Position - sRNA 68 -- 86@LB@Position Seed - mRNA 288 -- 294@LB@Position Seed - sRNA 70 -- 76@LB@
75	0.003854	0.1809	b0606	ahpF	-8.17	0.297536	59 -- 82	64 -- 85	alkyl hydroperoxide reductase F52a subunit FAD/NAD(P)-binding	_____________58_______________________83\n_____________\|________________________\|\n5'-UCA...UUGCU___A___GA______UC_______CACCU...CGG-3'\n______________CUG_CGC__AAUACU__GGAAAUU\n______________\|\|\|_\|\|\|__\|\|:\|\|:__\|\|\|\|\|\|:\n______________GAC_GCG__UUGUGG__CCUUUAG\n3'-UUU...CUUAU_______G_______UC_______AACCG...GUG-5'\n_____________\|________________________\|\n_____________86_______________________63\n	76 -- 82	64 -- 70	-19.6667 kcal/mol	4.11652 kcal/mol	7.38387 kcal/mol	58 83@LB@ \| \|@LB@5'-UCA...UUGCU A GA UC CACCU...CGG-3'@LB@ CUG CGC AAUACU GGAAAUU@LB@ \|\|\| \|\|\| \|\|:\|\|: \|\|\|\|\|\|:@LB@ GAC GCG UUGUGG CCUUUAG@LB@3'-UUU...CUUAU G UC AACCG...GUG-5'@LB@ \| \|@LB@ 86 63@LB@@LB@@LB@Energy -8.17 kcal/mol@LB@Hybridization Energy -19.6667 kcal/mol@LB@Unfolding Energy - mRNA 4.11652 kcal/mol@LB@Unfolding Energy - sRNA 7.38387 kcal/mol@LB@@LB@Position - mRNA 59 -- 82@LB@Position - sRNA 64 -- 85@LB@Position Seed - mRNA 76 -- 82@LB@Position Seed - sRNA 64 -- 70@LB@
102	0.005508	0.1855	b0659	ybeY	-7.73	0.351978	30 -- 54	64 -- 89	ssRNA-specific endoribonuclease; 16S rRNA 3' end maturation and quality control co-endoribonuclease working with RNase R; rRNA transcription antitermination factor	_____________29_________________________55\n_____________\|__________________________\|\n5'-ACU...AUCGA__________________AA______AGCUU...CGC-3'\n______________AGUGCUG_GCCGAUGUCG__GAGAUC\n______________\|:\|:\|\|\|_\|\|\|:\|:::\|:__\|\|:\|\|\|\n______________UUAUGAC_CGGUUGUGGU__CUUUAG\n3'-UUU...CGCGC_______G__________CC______AACCG...GUG-5'\n_____________\|__________________________\|\n_____________90_________________________63\n	30 -- 36	83 -- 89	-23.8058 kcal/mol	8.19456 kcal/mol	7.88317 kcal/mol	29 55@LB@ \| \|@LB@5'-ACU...AUCGA AA AGCUU...CGC-3'@LB@ AGUGCUG GCCGAUGUCG GAGAUC@LB@ \|:\|:\|\|\| \|\|\|:\|:::\|: \|\|:\|\|\|@LB@ UUAUGAC CGGUUGUGGU CUUUAG@LB@3'-UUU...CGCGC G CC AACCG...GUG-5'@LB@ \| \|@LB@ 90 63@LB@@LB@@LB@Energy -7.73 kcal/mol@LB@Hybridization Energy -23.8058 kcal/mol@LB@Unfolding Energy - mRNA 8.19456 kcal/mol@LB@Unfolding Energy - sRNA 7.88317 kcal/mol@LB@@LB@Position - mRNA 30 -- 54@LB@Position - sRNA 64 -- 89@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 83 -- 89@LB@
185	0.01108	0.2089	b0662	ubiF	-8.33	0.279302	272 -- 292	6 -- 27	2-octaprenyl-3-methyl-6-methoxy-1 4-benzoquinol oxygenase	_____________271____________________293\n_____________\|______________________\|\n5'-AAU...GGGCU____________GA___GC___AACGGUGA-3'\n______________GGCACAGCAC_G__UUU__GGU\n______________\|\|\|\|\|\|\|\|\|\|_\|__\|\|\|__\|\|:\n______________CCGUGUCGUG_C__AAA__CCG\n3'-UUU...UGUUC__________A_AA___GU___GCGUG-5'\n_____________\|______________________\|\n_____________28_____________________5\n	275 -- 281	18 -- 24	-24.5826 kcal/mol	6.14687 kcal/mol	10.1088 kcal/mol	271 293@LB@ \| \|@LB@5'-AAU...GGGCU GA GC AACGGUGA-3'@LB@ GGCACAGCAC G UUU GGU@LB@ \|\|\|\|\|\|\|\|\|\| \| \|\|\| \|\|:@LB@ CCGUGUCGUG C AAA CCG@LB@3'-UUU...UGUUC A AA GU GCGUG-5'@LB@ \| \|@LB@ 28 5@LB@@LB@@LB@Energy -8.33 kcal/mol@LB@Hybridization Energy -24.5826 kcal/mol@LB@Unfolding Energy - mRNA 6.14687 kcal/mol@LB@Unfolding Energy - sRNA 10.1088 kcal/mol@LB@@LB@Position - mRNA 272 -- 292@LB@Position - sRNA 6 -- 27@LB@Position Seed - mRNA 275 -- 281@LB@Position Seed - sRNA 18 -- 24@LB@
128	0.007127	0.1961	b0696	kdpC	-9.30	0.187437	90 -- 106	44 -- 60	potassium translocating ATPase subunit C	_____________89________________107\n_____________\|_________________\|\n5'-GCG...GCCAU____________C____GGAUU...GGU-3'\n______________GUUGCGCCGUAA_UUAU\n______________\|:\|\|\|\|\|\|\|\|\|\|_\|\|\|\|\n______________CGACGCGGCAUU_AAUA\n3'-UUU...AGAAC____________U____AUACU...GUG-5'\n_____________\|_________________\|\n_____________61________________43\n	95 -- 101	49 -- 55	-22.6138 kcal/mol	6.57247 kcal/mol	6.74006 kcal/mol	89 107@LB@ \| \|@LB@5'-GCG...GCCAU C GGAUU...GGU-3'@LB@ GUUGCGCCGUAA UUAU@LB@ \|:\|\|\|\|\|\|\|\|\|\| \|\|\|\|@LB@ CGACGCGGCAUU AAUA@LB@3'-UUU...AGAAC U AUACU...GUG-5'@LB@ \| \|@LB@ 61 43@LB@@LB@@LB@Energy -9.30 kcal/mol@LB@Hybridization Energy -22.6138 kcal/mol@LB@Unfolding Energy - mRNA 6.57247 kcal/mol@LB@Unfolding Energy - sRNA 6.74006 kcal/mol@LB@@LB@Position - mRNA 90 -- 106@LB@Position - sRNA 44 -- 60@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 49 -- 55@LB@
5	5.466e-05	0.03849	b0713	ybgL	-10.83	0.096857	234 -- 242	53 -- 61	UPF0271 family protein	_____________233_______243\n_____________\|_________\|\n5'-GCC...GCGAA_________AGCGA...AUG-3'\n______________GGCUGCGCC\n______________\|\|\|\|\|\|\|\|\|\n______________CCGACGCGG\n3'-UUU...UAGAA_________CAUUU...GUG-5'\n_____________\|_________\|\n_____________62________52\n	236 -- 242	53 -- 59	-22.2033 kcal/mol	6.50503 kcal/mol	4.87107 kcal/mol	233 243@LB@ \| \|@LB@5'-GCC...GCGAA AGCGA...AUG-3'@LB@ GGCUGCGCC@LB@ \|\|\|\|\|\|\|\|\|@LB@ CCGACGCGG@LB@3'-UUU...UAGAA CAUUU...GUG-5'@LB@ \| \|@LB@ 62 52@LB@@LB@@LB@Energy -10.83 kcal/mol@LB@Hybridization Energy -22.2033 kcal/mol@LB@Unfolding Energy - mRNA 6.50503 kcal/mol@LB@Unfolding Energy - sRNA 4.87107 kcal/mol@LB@@LB@Position - mRNA 234 -- 242@LB@Position - sRNA 53 -- 61@LB@Position Seed - mRNA 236 -- 242@LB@Position Seed - sRNA 53 -- 59@LB@
67	0.003373	0.1727	b0723	sdhA	-10.29	0.122560	175 -- 204	36 -- 70	succinate dehydrogenase flavoprotein subunit	_____________174_________________________________205\n_____________\|___________________________________\|\n5'-GGU...UUUAU___U____________G______GUG_________AAUUG...GUG-3'\n______________GGA__UC___GUUGUG_UGUGGG____UGUGAUGA\n______________\|\|\|__\|\|___\|:\|\|:\|_:\|\|:::____\|:\|\|\|\|\|\|\n______________CCU__AG___CGACGC_GCAUUU____AUACUACU\n3'-UUU...UGGUC___UU__AAC______G______AAUA________CAAUG...GUG-5'\n_____________\|___________________________________\|\n_____________71__________________________________35\n	198 -- 204	36 -- 42	-24.7755 kcal/mol	5.81881 kcal/mol	8.66537 kcal/mol	174 205@LB@ \| \|@LB@5'-GGU...UUUAU U G GUG AAUUG...GUG-3'@LB@ GGA UC GUUGUG UGUGGG UGUGAUGA@LB@ \|\|\| \|\| \|:\|\|:\| :\|\|::: \|:\|\|\|\|\|\|@LB@ CCU AG CGACGC GCAUUU AUACUACU@LB@3'-UUU...UGGUC UU AAC G AAUA CAAUG...GUG-5'@LB@ \| \|@LB@ 71 35@LB@@LB@@LB@Energy -10.29 kcal/mol@LB@Hybridization Energy -24.7755 kcal/mol@LB@Unfolding Energy - mRNA 5.81881 kcal/mol@LB@Unfolding Energy - sRNA 8.66537 kcal/mol@LB@@LB@Position - mRNA 175 -- 204@LB@Position - sRNA 36 -- 70@LB@Position Seed - mRNA 198 -- 204@LB@Position Seed - sRNA 36 -- 42@LB@
168	0.009959	0.2071	b0727	sucB	-11.51	0.071542	30 -- 47	72 -- 88	dihydrolipoyltranssuccinase	_____________29_________________48\n_____________\|__________________\|\n5'-CAG...GCAUG_______A__________UUUCC...GUG-3'\n______________GUACUGC_GCCAGCAUCA\n______________:\|\|\|\|\|\|_\|\|\|\|:\|\|:\|\|\n______________UAUGACG_CGGUUGUGGU\n3'-UUU...GCGCU__________________CCCUU...GUG-5'\n_____________\|__________________\|\n_____________89_________________71\n	38 -- 44	75 -- 81	-25.7274 kcal/mol	6.97777 kcal/mol	7.24148 kcal/mol	29 48@LB@ \| \|@LB@5'-CAG...GCAUG A UUUCC...GUG-3'@LB@ GUACUGC GCCAGCAUCA@LB@ :\|\|\|\|\|\| \|\|\|\|:\|\|:\|\|@LB@ UAUGACG CGGUUGUGGU@LB@3'-UUU...GCGCU CCCUU...GUG-5'@LB@ \| \|@LB@ 89 71@LB@@LB@@LB@Energy -11.51 kcal/mol@LB@Hybridization Energy -25.7274 kcal/mol@LB@Unfolding Energy - mRNA 6.97777 kcal/mol@LB@Unfolding Energy - sRNA 7.24148 kcal/mol@LB@@LB@Position - mRNA 30 -- 47@LB@Position - sRNA 72 -- 88@LB@Position Seed - mRNA 38 -- 44@LB@Position Seed - sRNA 75 -- 81@LB@
195	0.01197	0.2159	b0738	tolR	-7.83	0.338188	180 -- 193	26 -- 40	membrane spanning protein in TolA-TolQ-TolR complex	_____________179_____________194\n_____________\|_______________\|\n5'-UGG...UUAGC______CA_______GUAAG...UGG-3'\n______________GA_GAG__ACAAGGG\n______________\|\|_\|\|\|__\|\|\|\|\|\|\|\n______________CU_CUC__UGUUCCC\n3'-UUU...UAAUA__A___AA_______GUGUC...GUG-5'\n_____________\|_______________\|\n_____________41______________25\n	187 -- 193	26 -- 32	-14.5104 kcal/mol	3.98616 kcal/mol	2.69022 kcal/mol	179 194@LB@ \| \|@LB@5'-UGG...UUAGC CA GUAAG...UGG-3'@LB@ GA GAG ACAAGGG@LB@ \|\| \|\|\| \|\|\|\|\|\|\|@LB@ CU CUC UGUUCCC@LB@3'-UUU...UAAUA A AA GUGUC...GUG-5'@LB@ \| \|@LB@ 41 25@LB@@LB@@LB@Energy -7.83 kcal/mol@LB@Hybridization Energy -14.5104 kcal/mol@LB@Unfolding Energy - mRNA 3.98616 kcal/mol@LB@Unfolding Energy - sRNA 2.69022 kcal/mol@LB@@LB@Position - mRNA 180 -- 193@LB@Position - sRNA 26 -- 40@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 26 -- 32@LB@
99	0.005465	0.1855	b0767	pgl	-9.37	0.182441	275 -- 291	70 -- 86	6-phosphogluconolactonase	_____________274________________292\n_____________\|__________________\|\n5'-UCA...GGCGC____A_____________UUGUC...AUG-3'\n______________ACUG_CGCUGACA_CAGG\n______________\|\|\|\|_\|\|\|::\|\|\|_\|\|\|\|\n______________UGAC_GCGGUUGU_GUCC\n3'-UUU...GCUUA_____________G____CUUUA...GUG-5'\n_____________\|__________________\|\n_____________87_________________69\n	281 -- 287	75 -- 81	-21.4952 kcal/mol	4.85205 kcal/mol	7.27772 kcal/mol	274 292@LB@ \| \|@LB@5'-UCA...GGCGC A UUGUC...AUG-3'@LB@ ACUG CGCUGACA CAGG@LB@ \|\|\|\| \|\|\|::\|\|\| \|\|\|\|@LB@ UGAC GCGGUUGU GUCC@LB@3'-UUU...GCUUA G CUUUA...GUG-5'@LB@ \| \|@LB@ 87 69@LB@@LB@@LB@Energy -9.37 kcal/mol@LB@Hybridization Energy -21.4952 kcal/mol@LB@Unfolding Energy - mRNA 4.85205 kcal/mol@LB@Unfolding Energy - sRNA 7.27772 kcal/mol@LB@@LB@Position - mRNA 275 -- 291@LB@Position - sRNA 70 -- 86@LB@Position Seed - mRNA 281 -- 287@LB@Position Seed - sRNA 75 -- 81@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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