Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
31	0.001974	0.2094	b1080	flgI	-10.59	0.096403	87 -- 101	7 -- 21	putative flagellar basal body protein		_____________86______________102\n_____________\|_______________\|\n5'-AUU...ACUCA_______________CAUUG...AAA-3'\n______________GGUGGCGGAUGCGCG\n______________\|:\|::\|::\|\|\|\|\|\|\|\n______________CUAUUGUUUACGCGC\n3'-UUU...UACUA_______________AGAAAG-5'\n_____________\|_______________\|\n_____________22______________6\n	95 -- 101	7 -- 13	-22.6939 kcal/mol	10.8668 kcal/mol	1.24179 kcal/mol	86 102@LB@ \| \|@LB@5'-AUU...ACUCA CAUUG...AAA-3'@LB@ GGUGGCGGAUGCGCG@LB@ \|:\|::\|::\|\|\|\|\|\|\|@LB@ CUAUUGUUUACGCGC@LB@3'-UUU...UACUA AGAAAG-5'@LB@ \| \|@LB@ 22 6@LB@@LB@@LB@Energy -10.59 kcal/mol@LB@Hybridization Energy -22.6939 kcal/mol@LB@Unfolding Energy - mRNA 10.8668 kcal/mol@LB@Unfolding Energy - sRNA 1.24179 kcal/mol@LB@@LB@Position - mRNA 87 -- 101@LB@Position - sRNA 7 -- 21@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 7 -- 13@LB@
32	0.001981	0.2094	b3110	yhaO	-10.61	0.095420	268 -- 283	7 -- 23	putative transporter		_____________267_______________284\n_____________\|_________________\|\n5'-AAU...AAUGA____G____________AGAAG...UCG-3'\n______________GUGA_AGCGAGUG_GCG\n______________:\|\|\|_\|:\|:\|:\|\|_\|\|\|\n______________UACU_UUGUUUAC_CGC\n3'-UUU...CCUAC____A________G___AGAAAG-5'\n_____________\|_________________\|\n_____________24________________6\n	274 -- 280	11 -- 17	-15.5882 kcal/mol	2.45682 kcal/mol	2.52012 kcal/mol	267 284@LB@ \| \|@LB@5'-AAU...AAUGA G AGAAG...UCG-3'@LB@ GUGA AGCGAGUG GCG@LB@ :\|\|\| \|:\|:\|:\|\| \|\|\|@LB@ UACU UUGUUUAC CGC@LB@3'-UUU...CCUAC A G AGAAAG-5'@LB@ \| \|@LB@ 24 6@LB@@LB@@LB@Energy -10.61 kcal/mol@LB@Hybridization Energy -15.5882 kcal/mol@LB@Unfolding Energy - mRNA 2.45682 kcal/mol@LB@Unfolding Energy - sRNA 2.52012 kcal/mol@LB@@LB@Position - mRNA 268 -- 283@LB@Position - sRNA 7 -- 23@LB@Position Seed - mRNA 274 -- 280@LB@Position Seed - sRNA 11 -- 17@LB@
33	0.002096	0.2094	b0937	ssuE	-11.36	0.070700	201 -- 209	5 -- 13	NAD(P)H-dependent FMN reductase		_____________200_______210\n_____________\|_________\|\n5'-CUG...AGAGU_________AUCAC...AGG-3'\n______________AUGCGUGUC\n______________\|\|\|\|\|:\|\|\|\n______________UACGCGCAG\n3'-UUU...UUGUU_________AAAG-5'\n_____________\|_________\|\n_____________14________4\n	203 -- 209	5 -- 11	-14.6108 kcal/mol	2.09491 kcal/mol	1.15134 kcal/mol	200 210@LB@ \| \|@LB@5'-CUG...AGAGU AUCAC...AGG-3'@LB@ AUGCGUGUC@LB@ \|\|\|\|\|:\|\|\|@LB@ UACGCGCAG@LB@3'-UUU...UUGUU AAAG-5'@LB@ \| \|@LB@ 14 4@LB@@LB@@LB@Energy -11.36 kcal/mol@LB@Hybridization Energy -14.6108 kcal/mol@LB@Unfolding Energy - mRNA 2.09491 kcal/mol@LB@Unfolding Energy - sRNA 1.15134 kcal/mol@LB@@LB@Position - mRNA 201 -- 209@LB@Position - sRNA 5 -- 13@LB@Position Seed - mRNA 203 -- 209@LB@Position Seed - sRNA 5 -- 11@LB@
34	0.002145	0.2094	b1939	fliG	-13.29	0.032191	145 -- 162	6 -- 22	flagellar motor switching and energizing component		_____________144________________163\n_____________\|__________________\|\n5'-AGC...AUGUC_________UCC______GGUGG...AAG-3'\n______________UGAUAACGA___GCGCGU\n______________\|\|\|\|\|\|\|:\|___\|\|\|\|\|\|\n______________ACUAUUGUU___CGCGCA\n3'-UUU...CUACU_________UA_______GAAAG-5'\n_____________\|__________________\|\n_____________23_________________5\n	147 -- 153	14 -- 20	-19.5862 kcal/mol	5.04831 kcal/mol	1.24718 kcal/mol	144 163@LB@ \| \|@LB@5'-AGC...AUGUC UCC GGUGG...AAG-3'@LB@ UGAUAACGA GCGCGU@LB@ \|\|\|\|\|\|\|:\| \|\|\|\|\|\|@LB@ ACUAUUGUU CGCGCA@LB@3'-UUU...CUACU UA GAAAG-5'@LB@ \| \|@LB@ 23 5@LB@@LB@@LB@Energy -13.29 kcal/mol@LB@Hybridization Energy -19.5862 kcal/mol@LB@Unfolding Energy - mRNA 5.04831 kcal/mol@LB@Unfolding Energy - sRNA 1.24718 kcal/mol@LB@@LB@Position - mRNA 145 -- 162@LB@Position - sRNA 6 -- 22@LB@Position Seed - mRNA 147 -- 153@LB@Position Seed - sRNA 14 -- 20@LB@
35	0.002165	0.2094	b2714	ascG	-14.61	0.018547	188 -- 213	10 -- 30	asc operon transcriptional repressor; prpBC operon repressor		_____________187________________________214\n_____________\|__________________________\|\n5'-AAA...UGUAA________AAA_________CG____UGGAA...AAG-3'\n______________AGGGAUGA___UGAUGACGA__AUGC\n______________\|\|\|\|\|\|\|\|___\|\|\|\|:\|\|:\|__\|\|\|\|\n______________UCCCUACU___ACUAUUGUU__UACG\n3'-UUU...UUAAG__________________________CGCAG...AAG-5'\n_____________\|__________________________\|\n_____________31_________________________9\n	201 -- 207	14 -- 20	-25.4703 kcal/mol	3.44882 kcal/mol	7.41066 kcal/mol	187 214@LB@ \| \|@LB@5'-AAA...UGUAA AAA CG UGGAA...AAG-3'@LB@ AGGGAUGA UGAUGACGA AUGC@LB@ \|\|\|\|\|\|\|\| \|\|\|\|:\|\|:\| \|\|\|\|@LB@ UCCCUACU ACUAUUGUU UACG@LB@3'-UUU...UUAAG CGCAG...AAG-5'@LB@ \| \|@LB@ 31 9@LB@@LB@@LB@Energy -14.61 kcal/mol@LB@Hybridization Energy -25.4703 kcal/mol@LB@Unfolding Energy - mRNA 3.44882 kcal/mol@LB@Unfolding Energy - sRNA 7.41066 kcal/mol@LB@@LB@Position - mRNA 188 -- 213@LB@Position - sRNA 10 -- 30@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 14 -- 20@LB@
36	0.002407	0.2206	b2550	yphH	-13.17	0.033778	238 -- 251	7 -- 20	putative DNA-binding transcriptional regulator		_____________237____________252\n_____________\|______________\|\n5'-AGG...CCACC______________AUUCU...UGG-3'\n______________AUAACAAAUGCGUG\n______________\|\|\|\|\|\|\|\|\|\|\|\|:\|\n______________UAUUGUUUACGCGC\n3'-UUU...ACUAC______________AGAAAG-5'\n_____________\|______________\|\n_____________21_____________6\n	245 -- 251	7 -- 13	-19.0929 kcal/mol	4.67933 kcal/mol	1.23906 kcal/mol	237 252@LB@ \| \|@LB@5'-AGG...CCACC AUUCU...UGG-3'@LB@ AUAACAAAUGCGUG@LB@ \|\|\|\|\|\|\|\|\|\|\|\|:\|@LB@ UAUUGUUUACGCGC@LB@3'-UUU...ACUAC AGAAAG-5'@LB@ \| \|@LB@ 21 6@LB@@LB@@LB@Energy -13.17 kcal/mol@LB@Hybridization Energy -19.0929 kcal/mol@LB@Unfolding Energy - mRNA 4.67933 kcal/mol@LB@Unfolding Energy - sRNA 1.23906 kcal/mol@LB@@LB@Position - mRNA 238 -- 251@LB@Position - sRNA 7 -- 20@LB@Position Seed - mRNA 245 -- 251@LB@Position Seed - sRNA 7 -- 13@LB@
37	0.00241	0.2206	b2455	eutE	-11.61	0.064061	286 -- 298	3 -- 15	aldehyde oxidoreductase ethanolamine utilization protein		_____________285___________299\n_____________\|_____________\|\n5'-GGU...UCAUG_____G_______CG-3'\n______________AGAUG_GCGUUUU\n______________\|:\|\|\|_\|\|\|\|:\|\|\n______________UUUAC_CGCAGAA\n3'-UUU...UAUUG_____G_______AG-5'\n_____________\|_____________\|\n_____________16____________2\n	292 -- 298	3 -- 9	-14.5549 kcal/mol	1.73631 kcal/mol	1.20903 kcal/mol	285 299@LB@ \| \|@LB@5'-GGU...UCAUG G CG-3'@LB@ AGAUG GCGUUUU@LB@ \|:\|\|\| \|\|\|\|:\|\|@LB@ UUUAC CGCAGAA@LB@3'-UUU...UAUUG G AG-5'@LB@ \| \|@LB@ 16 2@LB@@LB@@LB@Energy -11.61 kcal/mol@LB@Hybridization Energy -14.5549 kcal/mol@LB@Unfolding Energy - mRNA 1.73631 kcal/mol@LB@Unfolding Energy - sRNA 1.20903 kcal/mol@LB@@LB@Position - mRNA 286 -- 298@LB@Position - sRNA 3 -- 15@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 3 -- 9@LB@
38	0.002512	0.2224	b0436	tig	-7.88	0.265562	192 -- 204	10 -- 21	peptidyl-prolyl cis/trans isomerase (trigger factor)		_____________191___________205\n_____________\|_____________\|\n5'-UAC...UUUGA_______A_____AAGUU...UCA-3'\n______________GGUAACA_GAUGC\n______________\|:\|\|\|\|\|_:\|\|\|\|\n______________CUAUUGU_UUACG\n3'-UUU...UACUA_____________CGCAG...AAG-5'\n_____________\|_____________\|\n_____________22____________9\n	192 -- 198	15 -- 21	-12.2618 kcal/mol	3.15229 kcal/mol	1.23076 kcal/mol	191 205@LB@ \| \|@LB@5'-UAC...UUUGA A AAGUU...UCA-3'@LB@ GGUAACA GAUGC@LB@ \|:\|\|\|\|\| :\|\|\|\|@LB@ CUAUUGU UUACG@LB@3'-UUU...UACUA CGCAG...AAG-5'@LB@ \| \|@LB@ 22 9@LB@@LB@@LB@Energy -7.88 kcal/mol@LB@Hybridization Energy -12.2618 kcal/mol@LB@Unfolding Energy - mRNA 3.15229 kcal/mol@LB@Unfolding Energy - sRNA 1.23076 kcal/mol@LB@@LB@Position - mRNA 192 -- 204@LB@Position - sRNA 10 -- 21@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 15 -- 21@LB@
39	0.002561	0.2224	b3845	fadA	-14.30	0.021162	112 -- 126	5 -- 20	3-ketoacyl-CoA thiolase (thiolase I)		_____________111______________127\n_____________\|________________\|\n5'-UCC...UCUGC________________AUAAC...AUU-3'\n______________GUAAUAAA_GCGCGUC\n______________:\|\|\|:\|\|\|_\|\|\|\|\|\|\|\n______________UAUUGUUU_CGCGCAG\n3'-UUU...ACUAC________A_______AAAG-5'\n_____________\|________________\|\n_____________21_______________4\n	120 -- 126	5 -- 11	-19.3309 kcal/mol	3.78617 kcal/mol	1.24804 kcal/mol	111 127@LB@ \| \|@LB@5'-UCC...UCUGC AUAAC...AUU-3'@LB@ GUAAUAAA GCGCGUC@LB@ :\|\|\|:\|\|\| \|\|\|\|\|\|\|@LB@ UAUUGUUU CGCGCAG@LB@3'-UUU...ACUAC A AAAG-5'@LB@ \| \|@LB@ 21 4@LB@@LB@@LB@Energy -14.30 kcal/mol@LB@Hybridization Energy -19.3309 kcal/mol@LB@Unfolding Energy - mRNA 3.78617 kcal/mol@LB@Unfolding Energy - sRNA 1.24804 kcal/mol@LB@@LB@Position - mRNA 112 -- 126@LB@Position - sRNA 5 -- 20@LB@Position Seed - mRNA 120 -- 126@LB@Position Seed - sRNA 5 -- 11@LB@
40	0.002726	0.2279	b1799	dmlR	-13.48	0.029707	223 -- 239	2 -- 20	transcriptional activator of dmlA	b3060	_____________222_________________240\n_____________\|___________________\|\n5'-CGC...GCUGA_____U_____________AUGCU...CGG-3'\n______________AUGAU___UGCGCGUCUUU\n______________\|\|:\|:___\|\|\|\|\|\|\|\|\|\|\|\n______________UAUUG___ACGCGCAGAAA\n3'-UUU...ACUAC_____UUU___________G-5'\n_____________\|___________________\|\n_____________21__________________1\n	233 -- 239	2 -- 8	-19.9945 kcal/mol	5.2543 kcal/mol	1.25604 kcal/mol	222 240@LB@ \| \|@LB@5'-CGC...GCUGA U AUGCU...CGG-3'@LB@ AUGAU UGCGCGUCUUU@LB@ \|\|:\|: \|\|\|\|\|\|\|\|\|\|\|@LB@ UAUUG ACGCGCAGAAA@LB@3'-UUU...ACUAC UUU G-5'@LB@ \| \|@LB@ 21 1@LB@@LB@@LB@Energy -13.48 kcal/mol@LB@Hybridization Energy -19.9945 kcal/mol@LB@Unfolding Energy - mRNA 5.2543 kcal/mol@LB@Unfolding Energy - sRNA 1.25604 kcal/mol@LB@@LB@Position - mRNA 223 -- 239@LB@Position - sRNA 2 -- 20@LB@Position Seed - mRNA 233 -- 239@LB@Position Seed - sRNA 2 -- 8@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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