Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
200	0.0195	0.3104	b3183	obgE	-7.01	0.355777	76 -- 83	4 -- 11	GTPase involved in cell partioning and DNA repair		_____________75_______84\n_____________\|________\|\n5'-UUU...UCUCG________GUCGU...GCC-3'\n______________GUGCGUUU\n______________\|:\|\|\|\|:\|\n______________CGCGCAGA\n3'-UUU...GUUUA________AAG-5'\n_____________\|________\|\n_____________12_______3\n	77 -- 83	4 -- 10	-11.428 kcal/mol	3.26207 kcal/mol	1.15639 kcal/mol	75 84@LB@ \| \|@LB@5'-UUU...UCUCG GUCGU...GCC-3'@LB@ GUGCGUUU@LB@ \|:\|\|\|\|:\|@LB@ CGCGCAGA@LB@3'-UUU...GUUUA AAG-5'@LB@ \| \|@LB@ 12 3@LB@@LB@@LB@Energy -7.01 kcal/mol@LB@Hybridization Energy -11.428 kcal/mol@LB@Unfolding Energy - mRNA 3.26207 kcal/mol@LB@Unfolding Energy - sRNA 1.15639 kcal/mol@LB@@LB@Position - mRNA 76 -- 83@LB@Position - sRNA 4 -- 11@LB@Position Seed - mRNA 77 -- 83@LB@Position Seed - sRNA 4 -- 10@LB@
164	0.0157	0.3104	b1735	chbR	-6.99	0.357617	139 -- 145	6 -- 12	repressor of chb operon for N N'-diacetylchitobiose utilization		_____________138_____146\n_____________\|_______\|\n5'-GAU...AUGCU_______GAACU...CUG-3'\n______________UGCGCGU\n______________\|\|\|\|\|\|\|\n______________ACGCGCA\n3'-UUU...UGUUU_______GAAAG-5'\n_____________\|_______\|\n_____________13______5\n	139 -- 145	6 -- 12	-12.5358 kcal/mol	4.43858 kcal/mol	1.10371 kcal/mol	138 146@LB@ \| \|@LB@5'-GAU...AUGCU GAACU...CUG-3'@LB@ UGCGCGU@LB@ \|\|\|\|\|\|\|@LB@ ACGCGCA@LB@3'-UUU...UGUUU GAAAG-5'@LB@ \| \|@LB@ 13 5@LB@@LB@@LB@Energy -6.99 kcal/mol@LB@Hybridization Energy -12.5358 kcal/mol@LB@Unfolding Energy - mRNA 4.43858 kcal/mol@LB@Unfolding Energy - sRNA 1.10371 kcal/mol@LB@@LB@Position - mRNA 139 -- 145@LB@Position - sRNA 6 -- 12@LB@Position Seed - mRNA 139 -- 145@LB@Position Seed - sRNA 6 -- 12@LB@
90	0.009145	0.3104	b1079	flgH	-6.81	0.379144	179 -- 204	10 -- 38	flagellar protein of basal-body outer-membrane L ring		_____________178___________________________205\n_____________\|_____________________________\|\n5'-ACU...GUUUA____U___U____A_________C_____AAAAA...GGG-3'\n______________CUGA_UUU___GA_GAUGAUAGC__AUGC\n______________\|\|\|\|_\|\|:___\|\|_\|\|\|\|\|\|\|:\|__\|\|\|\|\n______________GACU_AAG___CU_CUACUAUUG__UACG\n3'-UUU...GUAGA____U___UCC__A_________UU____CGCAG...AAG-5'\n_____________\|_____________________________\|\n_____________39____________________________9\n	193 -- 199	16 -- 22	-21.1251 kcal/mol	4.97135 kcal/mol	9.34299 kcal/mol	178 205@LB@ \| \|@LB@5'-ACU...GUUUA U U A C AAAAA...GGG-3'@LB@ CUGA UUU GA GAUGAUAGC AUGC@LB@ \|\|\|\| \|\|: \|\| \|\|\|\|\|\|\|:\| \|\|\|\|@LB@ GACU AAG CU CUACUAUUG UACG@LB@3'-UUU...GUAGA U UCC A UU CGCAG...AAG-5'@LB@ \| \|@LB@ 39 9@LB@@LB@@LB@Energy -6.81 kcal/mol@LB@Hybridization Energy -21.1251 kcal/mol@LB@Unfolding Energy - mRNA 4.97135 kcal/mol@LB@Unfolding Energy - sRNA 9.34299 kcal/mol@LB@@LB@Position - mRNA 179 -- 204@LB@Position - sRNA 10 -- 38@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 16 -- 22@LB@
68	0.006348	0.3087	b1202	ycgV	-6.40	0.429936	233 -- 244	4 -- 18	putative adhesin	b2647	_____________232_____________245\n_____________\|_______________\|\n5'-ACU...ACCAA_______________CGCUG...UUG-3'\n______________AGCAGAU___CGUCU\n______________\|:\|\|:\|\|___\|\|\|\|\|\n______________UUGUUUA___GCAGA\n3'-UUU...UACUA_______CGC_____AAG-5'\n_____________\|_______________\|\n_____________19______________3\n	233 -- 239	12 -- 18	-9.8712 kcal/mol	2.23703 kcal/mol	1.22995 kcal/mol	232 245@LB@ \| \|@LB@5'-ACU...ACCAA CGCUG...UUG-3'@LB@ AGCAGAU CGUCU@LB@ \|:\|\|:\|\| \|\|\|\|\|@LB@ UUGUUUA GCAGA@LB@3'-UUU...UACUA CGC AAG-5'@LB@ \| \|@LB@ 19 3@LB@@LB@@LB@Energy -6.40 kcal/mol@LB@Hybridization Energy -9.8712 kcal/mol@LB@Unfolding Energy - mRNA 2.23703 kcal/mol@LB@Unfolding Energy - sRNA 1.22995 kcal/mol@LB@@LB@Position - mRNA 233 -- 244@LB@Position - sRNA 4 -- 18@LB@Position Seed - mRNA 233 -- 239@LB@Position Seed - sRNA 12 -- 18@LB@
191	0.01886	0.3104	b3494	uspB	-6.32	0.440313	280 -- 286	5 -- 11	universal stress (ethanol tolerance) protein B		_____________279_____287\n_____________\|_______\|\n5'-AAU...ACUAC_______GUUAG...UGC-3'\n______________GCGCGUU\n______________\|\|\|\|\|\|:\n______________CGCGCAG\n3'-UUU...GUUUA_______AAAG-5'\n_____________\|_______\|\n_____________12______4\n	280 -- 286	5 -- 11	-11.0709 kcal/mol	3.63469 kcal/mol	1.11145 kcal/mol	279 287@LB@ \| \|@LB@5'-AAU...ACUAC GUUAG...UGC-3'@LB@ GCGCGUU@LB@ \|\|\|\|\|\|:@LB@ CGCGCAG@LB@3'-UUU...GUUUA AAAG-5'@LB@ \| \|@LB@ 12 4@LB@@LB@@LB@Energy -6.32 kcal/mol@LB@Hybridization Energy -11.0709 kcal/mol@LB@Unfolding Energy - mRNA 3.63469 kcal/mol@LB@Unfolding Energy - sRNA 1.11145 kcal/mol@LB@@LB@Position - mRNA 280 -- 286@LB@Position - sRNA 5 -- 11@LB@Position Seed - mRNA 280 -- 286@LB@Position Seed - sRNA 5 -- 11@LB@
184	0.01824	0.3104	b2055	wcaE	-6.17	0.461247	221 -- 230	4 -- 12	putative glycosyl transferase		_____________220________231\n_____________\|__________\|\n5'-ACU...AUCAC__U_______CGUAA...GCU-3'\n______________UG_CGCGUUU\n______________\|\|_\|\|\|\|\|:\|\n______________AC_GCGCAGA\n3'-UUU...UGUUU__________AAG-5'\n_____________\|__________\|\n_____________13_________3\n	224 -- 230	4 -- 10	-10.1102 kcal/mol	2.78254 kcal/mol	1.16019 kcal/mol	220 231@LB@ \| \|@LB@5'-ACU...AUCAC U CGUAA...GCU-3'@LB@ UG CGCGUUU@LB@ \|\| \|\|\|\|\|:\|@LB@ AC GCGCAGA@LB@3'-UUU...UGUUU AAG-5'@LB@ \| \|@LB@ 13 3@LB@@LB@@LB@Energy -6.17 kcal/mol@LB@Hybridization Energy -10.1102 kcal/mol@LB@Unfolding Energy - mRNA 2.78254 kcal/mol@LB@Unfolding Energy - sRNA 1.16019 kcal/mol@LB@@LB@Position - mRNA 221 -- 230@LB@Position - sRNA 4 -- 12@LB@Position Seed - mRNA 224 -- 230@LB@Position Seed - sRNA 4 -- 10@LB@
162	0.01506	0.3104	b0871	poxB	-6.02	0.481603	228 -- 255	23 -- 53	pyruvate dehydrogenase thiamine triphosphate-binding FAD-binding		_____________227_______________________________256\n_____________\|_________________________________\|\n5'-CCG...AUAUC_____________________CGG_________AACGC...GUG-3'\n______________GCCAAAA___CA_CUC_GAAU___CAGGGGUGA\n______________\|\|\|\|\|\|\|___\|\|_\|\|\|_\|\|\|\|___\|\|\|\|\|:\|\|\|\n______________CGGUUUU___GU_GAG_CUUA___GUCCCUACU\n3'-UUU...CUCAC_______AAA__A___A____A___________ACUAU...AAG-5'\n_____________\|_________________________________\|\n_____________54________________________________22\n	249 -- 255	23 -- 29	-28.0165 kcal/mol	6.84512 kcal/mol	15.1535 kcal/mol	227 256@LB@ \| \|@LB@5'-CCG...AUAUC CGG AACGC...GUG-3'@LB@ GCCAAAA CA CUC GAAU CAGGGGUGA@LB@ \|\|\|\|\|\|\| \|\| \|\|\| \|\|\|\| \|\|\|\|\|:\|\|\|@LB@ CGGUUUU GU GAG CUUA GUCCCUACU@LB@3'-UUU...CUCAC AAA A A A ACUAU...AAG-5'@LB@ \| \|@LB@ 54 22@LB@@LB@@LB@Energy -6.02 kcal/mol@LB@Hybridization Energy -28.0165 kcal/mol@LB@Unfolding Energy - mRNA 6.84512 kcal/mol@LB@Unfolding Energy - sRNA 15.1535 kcal/mol@LB@@LB@Position - mRNA 228 -- 255@LB@Position - sRNA 23 -- 53@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 23 -- 29@LB@
76	0.007084	0.3087	b4214	cysQ	-5.34	0.578728	11 -- 17	26 -- 32	3'(2') 5'-bisphosphate nucleotidase		_____________10______18\n_____________\|_______\|\n5'-AAC...AAUCA_______CAUCC...ACA-3'\n______________UCAGGGA\n______________\|\|\|\|\|\|\|\n______________AGUCCCU\n3'-UUU...ACUUA_______ACUAC...AAG-5'\n_____________\|_______\|\n_____________33______25\n	11 -- 17	26 -- 32	-11.6237 kcal/mol	0.931861 kcal/mol	5.35475 kcal/mol	10 18@LB@ \| \|@LB@5'-AAC...AAUCA CAUCC...ACA-3'@LB@ UCAGGGA@LB@ \|\|\|\|\|\|\|@LB@ AGUCCCU@LB@3'-UUU...ACUUA ACUAC...AAG-5'@LB@ \| \|@LB@ 33 25@LB@@LB@@LB@Energy -5.34 kcal/mol@LB@Hybridization Energy -11.6237 kcal/mol@LB@Unfolding Energy - mRNA 0.931861 kcal/mol@LB@Unfolding Energy - sRNA 5.35475 kcal/mol@LB@@LB@Position - mRNA 11 -- 17@LB@Position - sRNA 26 -- 32@LB@Position Seed - mRNA 11 -- 17@LB@Position Seed - sRNA 26 -- 32@LB@
192	0.01895	0.3104	b3333	gspL	-4.89	0.645256	110 -- 132	1 -- 21	general secretory pathway component cryptic		_____________109______________________133\n_____________\|________________________\|\n5'-GAU...CCAGG____________CAUAUU______AACCG...CGC-3'\n______________GAUGGCAAA_GC______UUUUUC\n______________\|\|\|::\|\|\|\|_\|\|______\|:\|\|\|\|\n______________CUAUUGUUU_CG______AGAAAG\n3'-UUU...UACUA_________A__CGC_________-5'\n_____________\|_______________________\|\n_____________22______________________1\n	110 -- 116	15 -- 21	-12.6455 kcal/mol	6.50221 kcal/mol	1.25763 kcal/mol	109 133@LB@ \| \|@LB@5'-GAU...CCAGG CAUAUU AACCG...CGC-3'@LB@ GAUGGCAAA GC UUUUUC@LB@ \|\|\|::\|\|\|\| \|\| \|:\|\|\|\|@LB@ CUAUUGUUU CG AGAAAG@LB@3'-UUU...UACUA A CGC -5'@LB@ \| \|@LB@ 22 1@LB@@LB@@LB@Energy -4.89 kcal/mol@LB@Hybridization Energy -12.6455 kcal/mol@LB@Unfolding Energy - mRNA 6.50221 kcal/mol@LB@Unfolding Energy - sRNA 1.25763 kcal/mol@LB@@LB@Position - mRNA 110 -- 132@LB@Position - sRNA 1 -- 21@LB@Position Seed - mRNA 110 -- 116@LB@Position Seed - sRNA 15 -- 21@LB@
140	0.01344	0.3104	b2388	glk	-4.25	0.738023	201 -- 214	10 -- 20	glucokinase		_____________200____________215\n_____________\|______________\|\n5'-CUA...GAAGA_______AGU____AUUAG...AUU-3'\n______________AUGACAA___AUGC\n______________\|\|:\|\|\|\|___\|\|\|\|\n______________UAUUGUU___UACG\n3'-UUU...ACUAC______________CGCAG...AAG-5'\n_____________\|______________\|\n_____________21_____________9\n	201 -- 207	14 -- 20	-8.41213 kcal/mol	2.93934 kcal/mol	1.22774 kcal/mol	200 215@LB@ \| \|@LB@5'-CUA...GAAGA AGU AUUAG...AUU-3'@LB@ AUGACAA AUGC@LB@ \|\|:\|\|\|\| \|\|\|\|@LB@ UAUUGUU UACG@LB@3'-UUU...ACUAC CGCAG...AAG-5'@LB@ \| \|@LB@ 21 9@LB@@LB@@LB@Energy -4.25 kcal/mol@LB@Hybridization Energy -8.41213 kcal/mol@LB@Unfolding Energy - mRNA 2.93934 kcal/mol@LB@Unfolding Energy - sRNA 1.22774 kcal/mol@LB@@LB@Position - mRNA 201 -- 214@LB@Position - sRNA 10 -- 20@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 14 -- 20@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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