Input and runtime details for job 3202356 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3202356 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:02 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:03:47 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:03:55 CET 2018
	Job Completed		@ Sat Feb 17 02:04:28 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3202356&toolName=CopraRNA ( 30 days expiry )

Description of the job

GcvB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
64	0.003108	0.1596	stm4162	thiF	-13.53	0.200092	247 -- 294	34 -- 76	molybdopterin/thiamine biosynthesis adenylyltransferase		_____________246___________________295\n_____________\|_____________________\|\n5'-GCA...GCUCG_______________A_____AGCAA...UUG-3'\n______________GCGAUAUCGCGAUUG_AGGCC\n______________:\|:\|:\|\|\|:\|:\|::\|_\|:\|\|\|\n______________UGUUGUAGUGUUGGC_UUCGG\n3'-UUU...UGUUG_______________A_____UUUUC...UCA-5'\n_____________\|_____________________\|\n_____________77____________________33\n	248 -- 254	69 -- 75	-25.2172 kcal/mol	7.66984 kcal/mol	8.92374 kcal/mol	246 295@LB@ \| \|@LB@5'-GCA...GCUCG A AGCAA...UUG-3'@LB@ GCGAUAUCGCGAUUG AGGCC@LB@ :\|:\|:\|\|\|:\|:\|::\| \|:\|\|\|@LB@ UGUUGUAGUGUUGGC UUCGG@LB@3'-UUU...UGUUG A UUUUC...UCA-5'@LB@ \| \|@LB@ 77 33@LB@@LB@@LB@Energy -13.53 kcal/mol@LB@Hybridization Energy -25.2172 kcal/mol@LB@Unfolding Energy - mRNA 7.66984 kcal/mol@LB@Unfolding Energy - sRNA 8.92374 kcal/mol@LB@@LB@Position - mRNA 247 -- 294@LB@Position - sRNA 34 -- 76@LB@Position Seed - mRNA 248 -- 254@LB@Position Seed - sRNA 69 -- 75@LB@
162	0.00849	0.1864	stm4221	pgi	-15.16	0.111294	249 -- 289	56 -- 88	glucose-6-phosphate isomerase		_____________248_______________________________________290\n_____________\|_________________________________________\|\n5'-GCU...UCCAG______U_____GAAAUGAAA_____________C______UUUCG...ACG-3'\n______________AAACAC_ACGAU_________GACGUUACGAUCG_UGAGCU\n______________\|\|\|\|\|\|_\|\|:\|:_________:\|\|:\|:\|\|:\|:\|\|_\|:\|\|\|:\n______________UUUGUG_UGUUG_________UUGUAGUGUUGGC_AUUCGG\n3'-UUU...UAACG______U_____UG___________________________UUUUC...UCA-5'\n_____________\|_________________________________________\|\n_____________89________________________________________55\n	272 -- 278	66 -- 72	-30.3131 kcal/mol	6.22888 kcal/mol	11.698 kcal/mol	248 290@LB@ \| \|@LB@5'-GCU...UCCAG U GAAAUGAAA C UUUCG...ACG-3'@LB@ AAACAC ACGAU GACGUUACGAUCG UGAGCU@LB@ \|\|\|\|\|\| \|\|:\|: :\|\|:\|:\|\|:\|:\|\| \|:\|\|\|:@LB@ UUUGUG UGUUG UUGUAGUGUUGGC AUUCGG@LB@3'-UUU...UAACG U UG UUUUC...UCA-5'@LB@ \| \|@LB@ 89 55@LB@@LB@@LB@Energy -15.16 kcal/mol@LB@Hybridization Energy -30.3131 kcal/mol@LB@Unfolding Energy - mRNA 6.22888 kcal/mol@LB@Unfolding Energy - sRNA 11.698 kcal/mol@LB@@LB@Position - mRNA 249 -- 289@LB@Position - sRNA 56 -- 88@LB@Position Seed - mRNA 272 -- 278@LB@Position Seed - sRNA 66 -- 72@LB@
46	0.001637	0.1267	stm2313	elaC	-17.21	0.051329	250 -- 300	51 -- 90	ribonuclease Z	stm1109	_____________249_____________________________________________301\n_____________\|_______________________________________________\|\n5'-UCG...CUCAC____UGUA________UUUAUUACAUCU________CAAC_______GGAG-3'\n______________GCAA____ACGGCAAU____________GCAACAUC____GCAGCCG\n______________\|\|\|\|____\|\|::\|\|\|:____________:\|\|\|\|\|\|\|____:\|\|:\|\|\|\n______________CGUU____UGUUGUUG____________UGUUGUAG____UGUUGGC\n3'-UUU...GUUAA____UG_________________________________________AUUCG...UCA-5'\n_____________\|_______________________________________________\|\n_____________91______________________________________________50\n	290 -- 296	62 -- 68	-28.5454 kcal/mol	7.10881 kcal/mol	8.62576 kcal/mol	249 301@LB@ \| \|@LB@5'-UCG...CUCAC UGUA UUUAUUACAUCU CAAC GGAG-3'@LB@ GCAA ACGGCAAU GCAACAUC GCAGCCG@LB@ \|\|\|\| \|\|::\|\|\|: :\|\|\|\|\|\|\| :\|\|:\|\|\|@LB@ CGUU UGUUGUUG UGUUGUAG UGUUGGC@LB@3'-UUU...GUUAA UG AUUCG...UCA-5'@LB@ \| \|@LB@ 91 50@LB@@LB@@LB@Energy -17.21 kcal/mol@LB@Hybridization Energy -28.5454 kcal/mol@LB@Unfolding Energy - mRNA 7.10881 kcal/mol@LB@Unfolding Energy - sRNA 8.62576 kcal/mol@LB@@LB@Position - mRNA 250 -- 300@LB@Position - sRNA 51 -- 90@LB@Position Seed - mRNA 290 -- 296@LB@Position Seed - sRNA 62 -- 68@LB@
77	0.003608	0.1596	stm4473	yjgM	-11.12	0.437983	252 -- 285	42 -- 82	GNAT family N-acetyltransferase		_____________251________________286\n_____________\|__________________\|\n5'-AUU...CGCCG________CC___C____AUGCC...ACG-3'\n______________AAUCACUA___GC_GACA\n______________\|\|\|\|\|\|\|\|___:\|_\|\|\|\|\n______________UUAGUGAU___UG_CUGU\n3'-UUU...UGUAA________ACC__U____CCCAU...UCA-5'\n_____________\|__________________\|\n_____________83_________________41\n	240 -- 246	165 -- 171	-13.0222 kcal/mol	3.39479 kcal/mol	3.49149 kcal/mol	251 286@LB@ \| \|@LB@5'-AUU...CGCCG CC C AUGCC...ACG-3'@LB@ AAUCACUA GC GACA@LB@ \|\|\|\|\|\|\|\| :\| \|\|\|\|@LB@ UUAGUGAU UG CUGU@LB@3'-UUU...UGUAA ACC U CCCAU...UCA-5'@LB@ \| \|@LB@ 83 41@LB@@LB@@LB@Energy -11.12 kcal/mol@LB@Hybridization Energy -13.0222 kcal/mol@LB@Unfolding Energy - mRNA 3.39479 kcal/mol@LB@Unfolding Energy - sRNA 3.49149 kcal/mol@LB@@LB@Position - mRNA 252 -- 285@LB@Position - sRNA 42 -- 82@LB@Position Seed - mRNA 240 -- 246@LB@Position Seed - sRNA 165 -- 171@LB@
150	0.007945	0.1845	stm2555	glyA	-15.42	0.100951	252 -- 282	137 -- 168	serine hydroxymethyltransferase		_____________251_________________________________283\n_____________\|___________________________________\|\n5'-CAC...GGCAG________________AA________GUC______AGCAC...UCG-3'\n______________GCUAUGGA___GCAGG__AAA_GUAC___AGGAAG\n______________:\|\|\|\|\|\|\|___:\|\|\|\|__\|\|\|_\|\|\|\|___\|\|\|\|\|\|\n______________UGAUACCU___UGUCC__UUU_CAUG___UCCUUC\n3'-UUU...AUUAG________GUC_____CA___A_____________ACUUU...UCA-5'\n_____________\|___________________________________\|\n_____________169_________________________________136\n	253 -- 259	161 -- 167	-28.2568 kcal/mol	5.17684 kcal/mol	6.91101 kcal/mol	251 283@LB@ \| \|@LB@5'-CAC...GGCAG AA GUC AGCAC...UCG-3'@LB@ GCUAUGGA GCAGG AAA GUAC AGGAAG@LB@ :\|\|\|\|\|\|\| :\|\|\|\| \|\|\| \|\|\|\| \|\|\|\|\|\|@LB@ UGAUACCU UGUCC UUU CAUG UCCUUC@LB@3'-UUU...AUUAG GUC CA A ACUUU...UCA-5'@LB@ \| \|@LB@ 169 136@LB@@LB@@LB@Energy -15.42 kcal/mol@LB@Hybridization Energy -28.2568 kcal/mol@LB@Unfolding Energy - mRNA 5.17684 kcal/mol@LB@Unfolding Energy - sRNA 6.91101 kcal/mol@LB@@LB@Position - mRNA 252 -- 282@LB@Position - sRNA 137 -- 168@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 161 -- 167@LB@
131	0.006551	0.1774	stm3217	aer	-17.88	0.039631	254 -- 298	36 -- 77	senses cellular redox state or proton motive force; similar to E. coli aerotaxis sensor receptor flavoprotein (AAC76107.1); Blastp hit to AAC76107.1 (506 aa) 83% identity in aa 1 - 506		_____________253____________________________________________________________________________________299\n_____________\|______________________________________________________________________________________\|\n5'-UUU...AAGGC______UGA_____________________ACAAA____AA__AUU____________________UC__G______UUUA_____UAAAG...CCA-3'\n______________GCAGGA___GGUGAAAA_________UCGU_____UAUU__GG____AAUCGCA____AAUUGCGA__CA_AGCAAU____ACAAC\n______________:\|\|\|\|\|___:\|\|\|\|\|\|\|_________\|\|\|:_____\|\|::__\|\|____\|\|\|\|\|\|\|____\|\|\|\|\|\|:\|__\|\|_\|:\|\|\|:____\|\|\|\|\|\n______________UGUCCU___UCACUUUU_________AGCG_____AUGG__CC____UUAGCGU____UUAACGUU__GU_UUGUUG____UGUUG\n3'-UUU...UUACA_________________UCCCAUCAG_____________CA__AGAC_______CUGG________U___G_______________UAGUG...UCA-5'\n_____________\|______________________________________________________________________________________\|\n_____________78_____________________________________________________________________________________35\n	89 -- 95	99 -- 105	-43.6348 kcal/mol	13.4466 kcal/mol	20.9633 kcal/mol	253 299@LB@ \| \|@LB@5'-UUU...AAGGC UGA ACAAA AA AUU UC G UUUA UAAAG...CCA-3'@LB@ GCAGGA GGUGAAAA UCGU UAUU GG AAUCGCA AAUUGCGA CA AGCAAU ACAAC@LB@ :\|\|\|\|\| :\|\|\|\|\|\|\| \|\|\|: \|\|:: \|\| \|\|\|\|\|\|\| \|\|\|\|\|\|:\| \|\| \|:\|\|\|: \|\|\|\|\|@LB@ UGUCCU UCACUUUU AGCG AUGG CC UUAGCGU UUAACGUU GU UUGUUG UGUUG@LB@3'-UUU...UUACA UCCCAUCAG CA AGAC CUGG U G UAGUG...UCA-5'@LB@ \| \|@LB@ 78 35@LB@@LB@@LB@Energy -17.88 kcal/mol@LB@Hybridization Energy -43.6348 kcal/mol@LB@Unfolding Energy - mRNA 13.4466 kcal/mol@LB@Unfolding Energy - sRNA 20.9633 kcal/mol@LB@@LB@Position - mRNA 254 -- 298@LB@Position - sRNA 36 -- 77@LB@Position Seed - mRNA 89 -- 95@LB@Position Seed - sRNA 99 -- 105@LB@
37	0.001263	0.1215	stm2511	guaB	-22.14	0.007548	255 -- 298	35 -- 73	IMP dehydrogenase		_____________254___________________________________________299\n_____________\|_____________________________________________\|\n5'-GGC...UUCCC_______UCC_____UUU______UACU___G___UC________GU-3'\n______________GC_UCAC___ACCGU___GCCGAA____GCC_AUC__AGCACGCA\n______________:\|_\|\|\|\|___\|\|\|\|\|___\|\|\|:\|\|____\|\|\|_\|\|\|__\|:\|\|\|\|\|\|\n______________UG_AGUG___UGGCA___CGGUUU____CGG_UAG__UUGUGCGU\n3'-UUU...UGUGU__U____U_______UU_______U______G___UC________AAGGC...UCA-5'\n_____________\|_____________________________________________\|\n_____________74____________________________________________34\n	292 -- 298	35 -- 41	-39.0524 kcal/mol	5.99157 kcal/mol	19.7557 kcal/mol	254 299@LB@ \| \|@LB@5'-GGC...UUCCC UCC UUU UACU G UC GU-3'@LB@ GC UCAC ACCGU GCCGAA GCC AUC AGCACGCA@LB@ :\| \|\|\|\| \|\|\|\|\| \|\|\|:\|\| \|\|\| \|\|\| \|:\|\|\|\|\|\|@LB@ UG AGUG UGGCA CGGUUU CGG UAG UUGUGCGU@LB@3'-UUU...UGUGU U U UU U G UC AAGGC...UCA-5'@LB@ \| \|@LB@ 74 34@LB@@LB@@LB@Energy -22.14 kcal/mol@LB@Hybridization Energy -39.0524 kcal/mol@LB@Unfolding Energy - mRNA 5.99157 kcal/mol@LB@Unfolding Energy - sRNA 19.7557 kcal/mol@LB@@LB@Position - mRNA 255 -- 298@LB@Position - sRNA 35 -- 73@LB@Position Seed - mRNA 292 -- 298@LB@Position Seed - sRNA 35 -- 41@LB@
111	0.005436	0.1728	stm2683	yfjB	-15.36	0.103307	255 -- 299	43 -- 90	NAD(+) kinase		_____________254___________________________________________________________________300\n_____________\|_____________________________________________________________________\|\n5'-CUC...UUCGC__________GAU____UU______UUCAA__________ACUCACAUU_____AUCAAUCU_______AAGGA...AGG-3'\n______________UUAUUAUGGG___CAGA__CAGGGU________GGGAAGU___________GUC________UCGCUAC\n______________\|:\|:\|\|\|\|\|:___\|\|\|\|__\|\|\|\|\|\|________:\|\|\|\|\|\|___________\|\|\|________\|\|\|\|\|\|\|\n______________AGUGAUACCU___GUCU__GUCCCA________UCCUUCA___________CAG________AGCGAUG\n3'-UUU...UAAUU_________________________UUUACAUG_______CUUUUUCCCAU__________________GCACC...UCA-5'\n_____________\|_____________________________________________________________________\|\n_____________91____________________________________________________________________42\n	179 -- 185	115 -- 121	-40.6869 kcal/mol	14.2494 kcal/mol	12.858 kcal/mol	254 300@LB@ \| \|@LB@5'-CUC...UUCGC GAU UU UUCAA ACUCACAUU AUCAAUCU AAGGA...AGG-3'@LB@ UUAUUAUGGG CAGA CAGGGU GGGAAGU GUC UCGCUAC@LB@ \|:\|:\|\|\|\|\|: \|\|\|\| \|\|\|\|\|\| :\|\|\|\|\|\| \|\|\| \|\|\|\|\|\|\|@LB@ AGUGAUACCU GUCU GUCCCA UCCUUCA CAG AGCGAUG@LB@3'-UUU...UAAUU UUUACAUG CUUUUUCCCAU GCACC...UCA-5'@LB@ \| \|@LB@ 91 42@LB@@LB@@LB@Energy -15.36 kcal/mol@LB@Hybridization Energy -40.6869 kcal/mol@LB@Unfolding Energy - mRNA 14.2494 kcal/mol@LB@Unfolding Energy - sRNA 12.858 kcal/mol@LB@@LB@Position - mRNA 255 -- 299@LB@Position - sRNA 43 -- 90@LB@Position Seed - mRNA 179 -- 185@LB@Position Seed - sRNA 115 -- 121@LB@
45	0.001635	0.1267	stm2351	hisP	-13.82	0.180688	258 -- 282	115 -- 140	histidine and lysine/arginine/ornithine transport protein		_____________257__________________________283\n_____________\|____________________________\|\n5'-UAC...GUCAU______CU____________________AGGCC...AUG-3'\n______________GAAGUG__GAAAGGGGUA___UCGCUGC\n______________\|\|\|\|\|\|__:\|\|\|:\|\|\|\|\|___\|\|\|\|\|:\|\n______________CUUCAC__UUUUUCCCAU___AGCGAUG\n3'-UUU...AUGUC__________________CAG_______GCACC...UCA-5'\n_____________\|____________________________\|\n_____________141__________________________114\n	276 -- 282	115 -- 121	-29.6028 kcal/mol	6.54228 kcal/mol	8.52785 kcal/mol	257 283@LB@ \| \|@LB@5'-UAC...GUCAU CU AGGCC...AUG-3'@LB@ GAAGUG GAAAGGGGUA UCGCUGC@LB@ \|\|\|\|\|\| :\|\|\|:\|\|\|\|\| \|\|\|\|\|:\|@LB@ CUUCAC UUUUUCCCAU AGCGAUG@LB@3'-UUU...AUGUC CAG GCACC...UCA-5'@LB@ \| \|@LB@ 141 114@LB@@LB@@LB@Energy -13.82 kcal/mol@LB@Hybridization Energy -29.6028 kcal/mol@LB@Unfolding Energy - mRNA 6.54228 kcal/mol@LB@Unfolding Energy - sRNA 8.52785 kcal/mol@LB@@LB@Position - mRNA 258 -- 282@LB@Position - sRNA 115 -- 140@LB@Position Seed - mRNA 276 -- 282@LB@Position Seed - sRNA 115 -- 121@LB@
120	0.006002	0.1758	stm2925	nlpD	-17.26	0.050371	258 -- 274	153 -- 170	lipoprotein NlpD		_____________257________________275\n_____________\|__________________\|\n5'-GUU...UGGUU___________U______UUGUA...CGG-3'\n______________UCACUAUGG_C_GGCAGG\n______________\|\|\|\|\|\|\|\|\|_\|_\|:\|\|\|\|\n______________AGUGAUACC_G_CUGUCC\n3'-UUU...UAAUU_________U_U______CAUUU...UCA-5'\n_____________\|__________________\|\n_____________171________________152\n	260 -- 266	162 -- 168	-24.411 kcal/mol	4.59255 kcal/mol	3.4766 kcal/mol	257 275@LB@ \| \|@LB@5'-GUU...UGGUU U UUGUA...CGG-3'@LB@ UCACUAUGG C GGCAGG@LB@ \|\|\|\|\|\|\|\|\| \| \|:\|\|\|\|@LB@ AGUGAUACC G CUGUCC@LB@3'-UUU...UAAUU U U CAUUU...UCA-5'@LB@ \| \|@LB@ 171 152@LB@@LB@@LB@Energy -17.26 kcal/mol@LB@Hybridization Energy -24.411 kcal/mol@LB@Unfolding Energy - mRNA 4.59255 kcal/mol@LB@Unfolding Energy - sRNA 3.4766 kcal/mol@LB@@LB@Position - mRNA 258 -- 274@LB@Position - sRNA 153 -- 170@LB@Position Seed - mRNA 260 -- 266@LB@Position Seed - sRNA 162 -- 168@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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