Input and runtime details for job 1786318 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1786318 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:20:27 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:20:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:01:46 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:01:53 CET 2018
	Job Completed		@ Fri Feb 16 22:02:22 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1786318&toolName=CopraRNA ( 30 days expiry )

Description of the job

ArcZ

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
180	0.01069	0.2089	b4529	ydbJ	-7.20	0.426635	222 -- 243	41 -- 61	DUF333 family putative lipoprotein		_____________221____________________244\n_____________\|______________________\|\n5'-GCA...GGGUA__G_______C_UU________CGGCG...GUU-3'\n______________GG_UGUGCCG_U__AUUGUUGU\n______________\|\|_\|\|:\|\|\|\|_\|__\|\|\|:\|\|:\|\n______________CC_ACGCGGC_A__UAAUAAUA\n3'-UUU...UAGAA__G_________UU________CUACU...GUG-5'\n_____________\|______________________\|\n_____________62_____________________40\n	225 -- 231	52 -- 58	-21.8046 kcal/mol	7.13894 kcal/mol	7.46625 kcal/mol	221 244@LB@ \| \|@LB@5'-GCA...GGGUA G C UU CGGCG...GUU-3'@LB@ GG UGUGCCG U AUUGUUGU@LB@ \|\| \|\|:\|\|\|\| \| \|\|\|:\|\|:\|@LB@ CC ACGCGGC A UAAUAAUA@LB@3'-UUU...UAGAA G UU CUACU...GUG-5'@LB@ \| \|@LB@ 62 40@LB@@LB@@LB@Energy -7.20 kcal/mol@LB@Hybridization Energy -21.8046 kcal/mol@LB@Unfolding Energy - mRNA 7.13894 kcal/mol@LB@Unfolding Energy - sRNA 7.46625 kcal/mol@LB@@LB@Position - mRNA 222 -- 243@LB@Position - sRNA 41 -- 61@LB@Position Seed - mRNA 225 -- 231@LB@Position Seed - sRNA 52 -- 58@LB@
172	0.0102	0.2071	b1383	ydbL	-9.96	0.141752	21 -- 31	52 -- 62	DUF1318 family protein		_____________20__________32\n_____________\|___________\|\n5'-AAA...UUUAC___________ACGUC...UAG-3'\n______________UGGCUGCGUUG\n______________\|\|\|\|\|\|\|\|::\|\n______________ACCGACGCGGC\n3'-UUU...UUAGA___________AUUUA...GUG-5'\n_____________\|___________\|\n_____________63__________51\n	25 -- 31	52 -- 58	-19.6889 kcal/mol	4.85225 kcal/mol	4.8804 kcal/mol	20 32@LB@ \| \|@LB@5'-AAA...UUUAC ACGUC...UAG-3'@LB@ UGGCUGCGUUG@LB@ \|\|\|\|\|\|\|\|::\|@LB@ ACCGACGCGGC@LB@3'-UUU...UUAGA AUUUA...GUG-5'@LB@ \| \|@LB@ 63 51@LB@@LB@@LB@Energy -9.96 kcal/mol@LB@Hybridization Energy -19.6889 kcal/mol@LB@Unfolding Energy - mRNA 4.85225 kcal/mol@LB@Unfolding Energy - sRNA 4.8804 kcal/mol@LB@@LB@Position - mRNA 21 -- 31@LB@Position - sRNA 52 -- 62@LB@Position Seed - mRNA 25 -- 31@LB@Position Seed - sRNA 52 -- 58@LB@
13	0.0004745	0.1198	b1688	ydiK	-12.82	0.039628	213 -- 226	68 -- 82	UPF0118 family inner membrane protein		_____________212_____________227\n_____________\|_______________\|\n5'-AUG...AUGUU_______________UGUCG...CCU-3'\n______________CGUCAGC_CCAGGGA\n______________\|\|:\|\|:\|_\|\|\|\|\|\|\|\n______________GCGGUUG_GGUCCCU\n3'-UUU...AUGAC_______U_______UUAGA...GUG-5'\n_____________\|_______________\|\n_____________83______________67\n	220 -- 226	68 -- 74	-23.7232 kcal/mol	3.92329 kcal/mol	6.98098 kcal/mol	212 227@LB@ \| \|@LB@5'-AUG...AUGUU UGUCG...CCU-3'@LB@ CGUCAGC CCAGGGA@LB@ \|\|:\|\|:\| \|\|\|\|\|\|\|@LB@ GCGGUUG GGUCCCU@LB@3'-UUU...AUGAC U UUAGA...GUG-5'@LB@ \| \|@LB@ 83 67@LB@@LB@@LB@Energy -12.82 kcal/mol@LB@Hybridization Energy -23.7232 kcal/mol@LB@Unfolding Energy - mRNA 3.92329 kcal/mol@LB@Unfolding Energy - sRNA 6.98098 kcal/mol@LB@@LB@Position - mRNA 213 -- 226@LB@Position - sRNA 68 -- 82@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 68 -- 74@LB@
113	0.005997	0.1855	b1765	ydjA	-10.36	0.119141	118 -- 144	49 -- 76	putative oxidoreductase		_____________117___________________________145\n_____________\|_____________________________\|\n5'-AUU...CUUGU__G__________GC__A___________CAAAA...GUA-3'\n______________CA_CAGG__AAUU__GG_CUGUGCUGUAA\n______________\|\|_\|\|\|\|__\|\|\|:__\|\|_\|\|\|:\|\|:\|\|\|\|\n______________GU_GUCC__UUAG__CC_GACGCGGCAUU\n3'-UUU...CGGUU__G____CU____AA______________UAAUA...GUG-5'\n_____________\|_____________________________\|\n_____________77____________________________48\n	138 -- 144	49 -- 55	-28.7704 kcal/mol	9.07167 kcal/mol	9.34253 kcal/mol	117 145@LB@ \| \|@LB@5'-AUU...CUUGU G GC A CAAAA...GUA-3'@LB@ CA CAGG AAUU GG CUGUGCUGUAA@LB@ \|\| \|\|\|\| \|\|\|: \|\| \|\|\|:\|\|:\|\|\|\|@LB@ GU GUCC UUAG CC GACGCGGCAUU@LB@3'-UUU...CGGUU G CU AA UAAUA...GUG-5'@LB@ \| \|@LB@ 77 48@LB@@LB@@LB@Energy -10.36 kcal/mol@LB@Hybridization Energy -28.7704 kcal/mol@LB@Unfolding Energy - mRNA 9.07167 kcal/mol@LB@Unfolding Energy - sRNA 9.34253 kcal/mol@LB@@LB@Position - mRNA 118 -- 144@LB@Position - sRNA 49 -- 76@LB@Position Seed - mRNA 138 -- 144@LB@Position Seed - sRNA 49 -- 55@LB@
117	0.006292	0.1893	b1769	ydjE	-12.05	0.056060	116 -- 139	36 -- 59	putative MFS sugar transporter membrane protein	b1775	_____________115______________________140\n_____________\|________________________\|\n5'-AUU...UGUUC_______U____A__AA_______UAAAG...UAA-3'\n______________CUGCGCC_UGAA_UA__AUGAUGA\n______________\|\|\|\|\|\|\|_\|:\|\|_\|\|__\|\|\|\|\|\|\|\n______________GACGCGG_AUUU_AU__UACUACU\n3'-UUU...GAACC_______C____A__AA_______CAAUG...GUG-5'\n_____________\|________________________\|\n_____________60_______________________35\n	133 -- 139	36 -- 42	-22.8542 kcal/mol	3.06151 kcal/mol	7.74108 kcal/mol	115 140@LB@ \| \|@LB@5'-AUU...UGUUC U A AA UAAAG...UAA-3'@LB@ CUGCGCC UGAA UA AUGAUGA@LB@ \|\|\|\|\|\|\| \|:\|\| \|\| \|\|\|\|\|\|\|@LB@ GACGCGG AUUU AU UACUACU@LB@3'-UUU...GAACC C A AA CAAUG...GUG-5'@LB@ \| \|@LB@ 60 35@LB@@LB@@LB@Energy -12.05 kcal/mol@LB@Hybridization Energy -22.8542 kcal/mol@LB@Unfolding Energy - mRNA 3.06151 kcal/mol@LB@Unfolding Energy - sRNA 7.74108 kcal/mol@LB@@LB@Position - mRNA 116 -- 139@LB@Position - sRNA 36 -- 59@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 36 -- 42@LB@
196	0.01202	0.2159	b1729	ydjN	-13.87	0.024594	143 -- 155	72 -- 84	putative transporter		_____________142___________156\n_____________\|_____________\|\n5'-CAU...AAUCU_____________UGACU...UGC-3'\n______________UGCGCCAACACUA\n______________\|\|\|\|\|\|\|\|\|\|\|:\|\n______________ACGCGGUUGUGGU\n3'-UUU...UUAUG_____________CCCUU...GUG-5'\n_____________\|_____________\|\n_____________85____________71\n	149 -- 155	72 -- 78	-23.7045 kcal/mol	2.6326 kcal/mol	7.20328 kcal/mol	142 156@LB@ \| \|@LB@5'-CAU...AAUCU UGACU...UGC-3'@LB@ UGCGCCAACACUA@LB@ \|\|\|\|\|\|\|\|\|\|\|:\|@LB@ ACGCGGUUGUGGU@LB@3'-UUU...UUAUG CCCUU...GUG-5'@LB@ \| \|@LB@ 85 71@LB@@LB@@LB@Energy -13.87 kcal/mol@LB@Hybridization Energy -23.7045 kcal/mol@LB@Unfolding Energy - mRNA 2.6326 kcal/mol@LB@Unfolding Energy - sRNA 7.20328 kcal/mol@LB@@LB@Position - mRNA 143 -- 155@LB@Position - sRNA 72 -- 84@LB@Position Seed - mRNA 149 -- 155@LB@Position Seed - sRNA 72 -- 78@LB@
7	0.0001601	0.08054	b1864	yebC	-15.40	0.012230	213 -- 249	54 -- 89	UPF0082 family protein		_____________212_____________________________________250\n_____________\|_______________________________________\|\n5'-CGU...GUCAU____AA__G___________AC_____GUAAA_______AGGAU...UAA-3'\n______________AGUA__UG_GCCAACACCAG____AUC_____GCUGCGC\n______________\|:\|\|__\|\|_\|\|\|\|\|\|\|\|\|\|\|____\|\|\|_____\|\|\|\|\|\|\|\n______________UUAU__AC_CGGUUGUGGUC____UAG_____CGACGCG\n3'-UUU...CGCGC____G___G___________CCUU___AAC_________GCAUU...GUG-5'\n_____________\|_______________________________________\|\n_____________90______________________________________53\n	243 -- 249	54 -- 60	-38.8431 kcal/mol	7.10336 kcal/mol	16.3388 kcal/mol	212 250@LB@ \| \|@LB@5'-CGU...GUCAU AA G AC GUAAA AGGAU...UAA-3'@LB@ AGUA UG GCCAACACCAG AUC GCUGCGC@LB@ \|:\|\| \|\| \|\|\|\|\|\|\|\|\|\|\| \|\|\| \|\|\|\|\|\|\|@LB@ UUAU AC CGGUUGUGGUC UAG CGACGCG@LB@3'-UUU...CGCGC G G CCUU AAC GCAUU...GUG-5'@LB@ \| \|@LB@ 90 53@LB@@LB@@LB@Energy -15.40 kcal/mol@LB@Hybridization Energy -38.8431 kcal/mol@LB@Unfolding Energy - mRNA 7.10336 kcal/mol@LB@Unfolding Energy - sRNA 16.3388 kcal/mol@LB@@LB@Position - mRNA 213 -- 249@LB@Position - sRNA 54 -- 89@LB@Position Seed - mRNA 243 -- 249@LB@Position Seed - sRNA 54 -- 60@LB@
175	0.01031	0.2074	b1834	yebT	-10.32	0.121003	270 -- 282	51 -- 62	MCE domain protein		_____________269___________283\n_____________\|_____________\|\n5'-UCU...CUUUC_______U_____UCAUC...UUG-3'\n______________UGGCUGC_GCCGU\n______________\|\|\|\|\|\|\|_\|\|\|\|\|\n______________ACCGACG_CGGCA\n3'-UUU...UUAGA_____________UUUAA...GUG-5'\n_____________\|_____________\|\n_____________63____________50\n	270 -- 276	56 -- 62	-21.4836 kcal/mol	6.28034 kcal/mol	4.8826 kcal/mol	269 283@LB@ \| \|@LB@5'-UCU...CUUUC U UCAUC...UUG-3'@LB@ UGGCUGC GCCGU@LB@ \|\|\|\|\|\|\| \|\|\|\|\|@LB@ ACCGACG CGGCA@LB@3'-UUU...UUAGA UUUAA...GUG-5'@LB@ \| \|@LB@ 63 50@LB@@LB@@LB@Energy -10.32 kcal/mol@LB@Hybridization Energy -21.4836 kcal/mol@LB@Unfolding Energy - mRNA 6.28034 kcal/mol@LB@Unfolding Energy - sRNA 4.8826 kcal/mol@LB@@LB@Position - mRNA 270 -- 282@LB@Position - sRNA 51 -- 62@LB@Position Seed - mRNA 270 -- 276@LB@Position Seed - sRNA 56 -- 62@LB@
164	0.009677	0.2068	b1956	yedQ	-11.74	0.064514	187 -- 209	18 -- 41	putative membrane-anchored diguanylate cyclase		_____________186______________________210\n_____________\|________________________\|\n5'-GAG...CAGGU________CGUC____________GAGAC...UAU-3'\n______________UGG_GAGU____AGGGUGCAGCAC\n______________\|\|:_\|\|\|\|____\|\|\|\|::\|\|\|\|\|\|\n______________ACU_CUCA____UCCCGUGUCGUG\n3'-UUU...AUAAU___A____AUGU____________ACAAA...GUG-5'\n_____________\|________________________\|\n_____________42_______________________17\n	203 -- 209	18 -- 24	-27.1151 kcal/mol	6.50018 kcal/mol	8.87585 kcal/mol	186 210@LB@ \| \|@LB@5'-GAG...CAGGU CGUC GAGAC...UAU-3'@LB@ UGG GAGU AGGGUGCAGCAC@LB@ \|\|: \|\|\|\| \|\|\|\|::\|\|\|\|\|\|@LB@ ACU CUCA UCCCGUGUCGUG@LB@3'-UUU...AUAAU A AUGU ACAAA...GUG-5'@LB@ \| \|@LB@ 42 17@LB@@LB@@LB@Energy -11.74 kcal/mol@LB@Hybridization Energy -27.1151 kcal/mol@LB@Unfolding Energy - mRNA 6.50018 kcal/mol@LB@Unfolding Energy - sRNA 8.87585 kcal/mol@LB@@LB@Position - mRNA 187 -- 209@LB@Position - sRNA 18 -- 41@LB@Position Seed - mRNA 203 -- 209@LB@Position Seed - sRNA 18 -- 24@LB@
4	3.471e-06	0.003055	b2099	yegU	-19.38	0.001997	173 -- 198	64 -- 88	ADP-ribosylglycohydrolase family protein		_____________172________________________199\n_____________\|__________________________\|\n5'-GUG...UCGCG_______________AA_________AAAUG...CAC-3'\n______________AUAUUGCGUUGACAC__GGGGAAGUU\n______________\|\|\|:\|\|\|\|:::\|\|\|\|__:\|\|\|\|\|:\|:\n______________UAUGACGCGGUUGUG__UCCCUUUAG\n3'-UUU...GCGCU_______________G__________AACCG...GUG-5'\n_____________\|__________________________\|\n_____________89_________________________63\n	192 -- 198	64 -- 70	-32.1286 kcal/mol	5.35868 kcal/mol	7.38555 kcal/mol	172 199@LB@ \| \|@LB@5'-GUG...UCGCG AA AAAUG...CAC-3'@LB@ AUAUUGCGUUGACAC GGGGAAGUU@LB@ \|\|\|:\|\|\|\|:::\|\|\|\| :\|\|\|\|\|:\|:@LB@ UAUGACGCGGUUGUG UCCCUUUAG@LB@3'-UUU...GCGCU G AACCG...GUG-5'@LB@ \| \|@LB@ 89 63@LB@@LB@@LB@Energy -19.38 kcal/mol@LB@Hybridization Energy -32.1286 kcal/mol@LB@Unfolding Energy - mRNA 5.35868 kcal/mol@LB@Unfolding Energy - sRNA 7.38555 kcal/mol@LB@@LB@Position - mRNA 173 -- 198@LB@Position - sRNA 64 -- 88@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 64 -- 70@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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