| 161 |
0.00741 |
0.1751 |
bsu24000 |
bmrU |
-16.51 |
0.015967 |
115 -- 144 |
156 -- 183 |
lipid kinase BmrU |
|
_____________114____________________________________145\n_____________|______________________________________|\n5'-GGA...AAUUA___________UC____AA_ACCUC_____________AUCUG...AUU-3'\n______________AAG_UCCAUGU__AAUG__G_____UC_CUUUUUCAUC\n______________|||_|||||:|__||||__|_____||_||||:|||||\n______________UUC_AGGUAUA__UUAC__C_____AG_GAAAGAGUAG\n3'-AUU...CGUCG___A_____________AA________U__________AAGUU...CAA-5'\n_____________|______________________________________|\n_____________184____________________________________155\n |
56 -- 62 |
102 -- 108 |
-19.6184 kcal/mol |
4.38872 kcal/mol |
6.78097 kcal/mol |
114 145@LB@ | |@LB@5'-GGA...AAUUA UC AA ACCUC AUCUG...AUU-3'@LB@ AAG UCCAUGU AAUG G UC CUUUUUCAUC@LB@ ||| |||||:| |||| | || ||||:|||||@LB@ UUC AGGUAUA UUAC C AG GAAAGAGUAG@LB@3'-AUU...CGUCG A AA U AAGUU...CAA-5'@LB@ | |@LB@ 184 155@LB@@LB@@LB@Energy -16.51 kcal/mol@LB@Hybridization Energy -19.6184 kcal/mol@LB@Unfolding Energy - mRNA 4.38872 kcal/mol@LB@Unfolding Energy - sRNA 6.78097 kcal/mol@LB@@LB@Position - mRNA 115 -- 144@LB@Position - sRNA 156 -- 183@LB@Position Seed - mRNA 56 -- 62@LB@Position Seed - sRNA 102 -- 108@LB@ |
| 162 |
0.007531 |
0.1751 |
bsu14350 |
yknX |
-8.74 |
0.495402 |
179 -- 195 |
123 -- 140 |
efflux system component YknX |
|
_____________178________________196\n_____________|__________________|\n5'-UGA...UCAAU____________G_____AGCUC...CCG-3'\n______________UCUGUGGCGGGU__CAUA\n______________||:|::||::||__||||\n______________AGGCGUCGUUCA__GUAU\n3'-AUU...GUAAC____________AG____AUUAC...CAA-5'\n_____________|__________________|\n_____________141________________122\n |
184 -- 190 |
129 -- 135 |
-18.5785 kcal/mol |
5.82762 kcal/mol |
6.13212 kcal/mol |
178 196@LB@ | |@LB@5'-UGA...UCAAU G AGCUC...CCG-3'@LB@ UCUGUGGCGGGU CAUA@LB@ ||:|::||::|| ||||@LB@ AGGCGUCGUUCA GUAU@LB@3'-AUU...GUAAC AG AUUAC...CAA-5'@LB@ | |@LB@ 141 122@LB@@LB@@LB@Energy -8.74 kcal/mol@LB@Hybridization Energy -18.5785 kcal/mol@LB@Unfolding Energy - mRNA 5.82762 kcal/mol@LB@Unfolding Energy - sRNA 6.13212 kcal/mol@LB@@LB@Position - mRNA 179 -- 195@LB@Position - sRNA 123 -- 140@LB@Position Seed - mRNA 184 -- 190@LB@Position Seed - sRNA 129 -- 135@LB@ |
| 163 |
0.007546 |
0.1751 |
bsu03210 |
ycgN |
-11.47 |
0.170088 |
123 -- 268 |
12 -- 147 |
1-pyrroline-5-carboxylate dehydrogenase |
|
_____________122______________________________________________________269\n_____________|________________________________________________________|\n5'-CAA...CCUUA__________A___AU__A_AA______AAGAUCAAAACAACG_____________AAAAG...UAG-3'\n______________CAAACA__CG_GCC__UC_C__AUUUCC_______________UGGAAGCGUUUAA\n______________||||||__||_:||__||_|__|||:||_______________||:||:||:||||\n______________GUUUGU__GC_UGG__AG_G__UAAGGG_______________ACUUUUGCGAAUU\n3'-AUU...GAACC______UA__A___GU__A_AA______GUAAAGAAACUUUAA_____________AAAGA...CAA-5'\n_____________|________________________________________________________|\n_____________148______________________________________________________11\n |
262 -- 268 |
12 -- 18 |
-30.9991 kcal/mol |
6.38057 kcal/mol |
15.527 kcal/mol |
122 269@LB@ | |@LB@5'-CAA...CCUUA A AU A AA AAGAUCAAAACAACG AAAAG...UAG-3'@LB@ CAAACA CG GCC UC C AUUUCC UGGAAGCGUUUAA@LB@ |||||| || :|| || | |||:|| ||:||:||:||||@LB@ GUUUGU GC UGG AG G UAAGGG ACUUUUGCGAAUU@LB@3'-AUU...GAACC UA A GU A AA GUAAAGAAACUUUAA AAAGA...CAA-5'@LB@ | |@LB@ 148 11@LB@@LB@@LB@Energy -11.47 kcal/mol@LB@Hybridization Energy -30.9991 kcal/mol@LB@Unfolding Energy - mRNA 6.38057 kcal/mol@LB@Unfolding Energy - sRNA 15.527 kcal/mol@LB@@LB@Position - mRNA 123 -- 268@LB@Position - sRNA 12 -- 147@LB@Position Seed - mRNA 262 -- 268@LB@Position Seed - sRNA 12 -- 18@LB@ |
| 164 |
0.007653 |
0.1751 |
bsu02110 |
ybyB |
-7.82 |
0.651200 |
271 -- 286 |
38 -- 56 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________270_____287\n_____________|_______|\n5'-UUG...AAAAG_______AAAUC...AAU-3'\n______________ACCCGUC\n______________||||:||\n______________UGGGUAG\n3'-AUU...UAGCA_______AGAAU...CAA-5'\n_____________|_______|\n_____________57______37\n |
271 -- 277 |
50 -- 56 |
-11.5721 kcal/mol |
0.816393 kcal/mol |
3.06627 kcal/mol |
270 287@LB@ | |@LB@5'-UUG...AAAAG AAAUC...AAU-3'@LB@ ACCCGUC@LB@ ||||:||@LB@ UGGGUAG@LB@3'-AUU...UAGCA AGAAU...CAA-5'@LB@ | |@LB@ 57 37@LB@@LB@@LB@Energy -7.82 kcal/mol@LB@Hybridization Energy -11.5721 kcal/mol@LB@Unfolding Energy - mRNA 0.816393 kcal/mol@LB@Unfolding Energy - sRNA 3.06627 kcal/mol@LB@@LB@Position - mRNA 271 -- 286@LB@Position - sRNA 38 -- 56@LB@Position Seed - mRNA 271 -- 277@LB@Position Seed - sRNA 50 -- 56@LB@ |
| 165 |
0.007704 |
0.1751 |
bsu18170 |
yngA |
-16.77 |
0.014091 |
132 -- 192 |
122 -- 181 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________131_____________________________________________193\n_____________|_______________________________________________|\n5'-ACA...CAUAU_________UGUU____CA___C_______UAU______________CAACA...CGC-3'\n______________UUUAUUAAU____UAUU__ACA_UGUCCGC___AGUAA__UCCAUAU\n______________||:||:||:____:|||__|||_|||||||___||:||__|||||||\n______________AAGUAGUUG____GUAA__UGU_ACAGGCG___UCGUU__AGGUAUA\n3'-AUU...AUGAG_________UCCU____C____A_______________CA_______UUACA...CAA-5'\n_____________|_______________________________________________|\n_____________182_____________________________________________121\n |
186 -- 192 |
122 -- 128 |
-30.4083 kcal/mol |
4.20108 kcal/mol |
10.3807 kcal/mol |
131 193@LB@ | |@LB@5'-ACA...CAUAU UGUU CA C UAU CAACA...CGC-3'@LB@ UUUAUUAAU UAUU ACA UGUCCGC AGUAA UCCAUAU@LB@ ||:||:||: :||| ||| ||||||| ||:|| |||||||@LB@ AAGUAGUUG GUAA UGU ACAGGCG UCGUU AGGUAUA@LB@3'-AUU...AUGAG UCCU C A CA UUACA...CAA-5'@LB@ | |@LB@ 182 121@LB@@LB@@LB@Energy -16.77 kcal/mol@LB@Hybridization Energy -30.4083 kcal/mol@LB@Unfolding Energy - mRNA 4.20108 kcal/mol@LB@Unfolding Energy - sRNA 10.3807 kcal/mol@LB@@LB@Position - mRNA 132 -- 192@LB@Position - sRNA 122 -- 181@LB@Position Seed - mRNA 186 -- 192@LB@Position Seed - sRNA 122 -- 128@LB@ |
| 166 |
0.007737 |
0.1751 |
bsu10770 |
wprA |
-9.60 |
0.364837 |
176 -- 232 |
111 -- 164 |
cell wall-associated protease |
|
_____________175_________________________________________________________233\n_____________|___________________________________________________________|\n5'-CUG...GGAGA_______UUUU______G_GAUA____GAAA_____AAAA_____GCUCG______G__GGCAG...GCG-3'\n______________UUCAUUA____GCAGGA_G____ACAU____CGCAG_____UUCA______GUUGU_GC\n______________|||||:|____:|||||_:____||||____|||||_____|:||______|||||_:|\n______________AAGUAGU____UGUCCU_U____UGUA____GCGUC_____AGGU______CAACA_UG\n3'-AUU...AUGAG_________________G_AAC_____ACAG_____GUUCA____AUAUUA_____G__AAAGA...CAA-5'\n_____________|___________________________________________________________|\n_____________165_________________________________________________________110\n |
176 -- 182 |
158 -- 164 |
-36.1081 kcal/mol |
15.1078 kcal/mol |
11.9485 kcal/mol |
175 233@LB@ | |@LB@5'-CUG...GGAGA UUUU G GAUA GAAA AAAA GCUCG G GGCAG...GCG-3'@LB@ UUCAUUA GCAGGA G ACAU CGCAG UUCA GUUGU GC@LB@ |||||:| :||||| : |||| ||||| |:|| ||||| :|@LB@ AAGUAGU UGUCCU U UGUA GCGUC AGGU CAACA UG@LB@3'-AUU...AUGAG G AAC ACAG GUUCA AUAUUA G AAAGA...CAA-5'@LB@ | |@LB@ 165 110@LB@@LB@@LB@Energy -9.60 kcal/mol@LB@Hybridization Energy -36.1081 kcal/mol@LB@Unfolding Energy - mRNA 15.1078 kcal/mol@LB@Unfolding Energy - sRNA 11.9485 kcal/mol@LB@@LB@Position - mRNA 176 -- 232@LB@Position - sRNA 111 -- 164@LB@Position Seed - mRNA 176 -- 182@LB@Position Seed - sRNA 158 -- 164@LB@ |
| 167 |
0.007775 |
0.1751 |
bsu09889 |
yhzD |
-15.75 |
0.023145 |
43 -- 92 |
39 -- 89 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________42____________________________________________________93\n_____________|_____________________________________________________|\n5'-GGA...CUCCA____U_______AC______AAUU_______C___A____C____C_______UGACC...GUG-3'\n______________AAUG_CUUCGUU__GUGAUC____GUAAGCA__UG_AUCU__CUC___UUCCC\n______________||||_|||||||__|::|||____|:||:||__:|_|:|:__|||___|||||\n______________UUAC_GAAGCAA__CGUUAG____CGUUUGU__GC_UGGG__GAG___AAGGG\n3'-AUU...AUUAC____C_______________AAC________UA__A____UA___AAU_____GUAAA...CAA-5'\n_____________|_____________________________________________________|\n_____________90____________________________________________________38\n |
67 -- 73 |
62 -- 68 |
-30.4166 kcal/mol |
5.34264 kcal/mol |
10.926 kcal/mol |
42 93@LB@ | |@LB@5'-GGA...CUCCA U AC AAUU C A C C UGACC...GUG-3'@LB@ AAUG CUUCGUU GUGAUC GUAAGCA UG AUCU CUC UUCCC@LB@ |||| ||||||| |::||| |:||:|| :| |:|: ||| |||||@LB@ UUAC GAAGCAA CGUUAG CGUUUGU GC UGGG GAG AAGGG@LB@3'-AUU...AUUAC C AAC UA A UA AAU GUAAA...CAA-5'@LB@ | |@LB@ 90 38@LB@@LB@@LB@Energy -15.75 kcal/mol@LB@Hybridization Energy -30.4166 kcal/mol@LB@Unfolding Energy - mRNA 5.34264 kcal/mol@LB@Unfolding Energy - sRNA 10.926 kcal/mol@LB@@LB@Position - mRNA 43 -- 92@LB@Position - sRNA 39 -- 89@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 62 -- 68@LB@ |
| 168 |
0.007777 |
0.1751 |
bsu32270 |
yutH |
-11.70 |
0.153975 |
226 -- 259 |
121 -- 158 |
endospore coat-associated protein YutH |
|
_____________225____________________________________260\n_____________|______________________________________|\n5'-UGC...AGAAA____C______C____CAG_______U___________CCAGC...ACU-3'\n______________AAUA_GGCAUU_ACAU_____GCAGC___UUCCAUGUA\n______________||:|_|:||||_||||_____|||||___||||||:||\n______________UUGU_CUGUAA_UGUA_____CGUCG___AAGGUAUAU\n3'-AUU...AGUAG____C______C____ACAGG_____UUC_________UACAA...CAA-5'\n_____________|______________________________________|\n_____________159____________________________________120\n |
253 -- 259 |
121 -- 127 |
-28.4364 kcal/mol |
6.47482 kcal/mol |
10.499 kcal/mol |
225 260@LB@ | |@LB@5'-UGC...AGAAA C C CAG U CCAGC...ACU-3'@LB@ AAUA GGCAUU ACAU GCAGC UUCCAUGUA@LB@ ||:| |:|||| |||| ||||| ||||||:||@LB@ UUGU CUGUAA UGUA CGUCG AAGGUAUAU@LB@3'-AUU...AGUAG C C ACAGG UUC UACAA...CAA-5'@LB@ | |@LB@ 159 120@LB@@LB@@LB@Energy -11.70 kcal/mol@LB@Hybridization Energy -28.4364 kcal/mol@LB@Unfolding Energy - mRNA 6.47482 kcal/mol@LB@Unfolding Energy - sRNA 10.499 kcal/mol@LB@@LB@Position - mRNA 226 -- 259@LB@Position - sRNA 121 -- 158@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 169 |
0.007812 |
0.1751 |
bsu04680 |
rsbS |
-7.92 |
0.633185 |
77 -- 93 |
53 -- 67 |
RsbT antagonist protein RsbS |
|
_____________76___________________94\n_____________|____________________|\n5'-UAU...GGAAA______AAACAA________CUGGG...AAG-3'\n______________UUGCUC______UCGUCAAC\n______________||:|||______||:|||||\n______________AAUGAG______AGUAGUUG\n3'-AUU...AAAAA______A_____________UCCUG...CAA-5'\n_____________|____________________|\n_____________68___________________52\n |
85 -- 91 |
156 -- 162 |
-12.3218 kcal/mol |
3.62054 kcal/mol |
1.40896 kcal/mol |
76 94@LB@ | |@LB@5'-UAU...GGAAA AAACAA CUGGG...AAG-3'@LB@ UUGCUC UCGUCAAC@LB@ ||:||| ||:|||||@LB@ AAUGAG AGUAGUUG@LB@3'-AUU...AAAAA A UCCUG...CAA-5'@LB@ | |@LB@ 68 52@LB@@LB@@LB@Energy -7.92 kcal/mol@LB@Hybridization Energy -12.3218 kcal/mol@LB@Unfolding Energy - mRNA 3.62054 kcal/mol@LB@Unfolding Energy - sRNA 1.40896 kcal/mol@LB@@LB@Position - mRNA 77 -- 93@LB@Position - sRNA 53 -- 67@LB@Position Seed - mRNA 85 -- 91@LB@Position Seed - sRNA 156 -- 162@LB@ |
| 170 |
0.007871 |
0.1751 |
bsu13520 |
ykrP |
-11.14 |
0.196181 |
76 -- 136 |
99 -- 167 |
membrane-bound acyltransferase YkrP |
|
_____________75____________137\n_____________|_____________|\n5'-GGA...CCGUC_____A_______CUAAC...AUC-3'\n______________ACUUU_CAUCGAC\n______________|||:|_||||:||\n______________UGAGA_GUAGUUG\n3'-AUU...AAAAA_____A_______UCCUG...CAA-5'\n_____________|_____________|\n_____________168___________98\n |
91 -- 97 |
156 -- 162 |
-12.1838 kcal/mol |
0.966795 kcal/mol |
1.40592 kcal/mol |
75 137@LB@ | |@LB@5'-GGA...CCGUC A CUAAC...AUC-3'@LB@ ACUUU CAUCGAC@LB@ |||:| ||||:||@LB@ UGAGA GUAGUUG@LB@3'-AUU...AAAAA A UCCUG...CAA-5'@LB@ | |@LB@ 168 98@LB@@LB@@LB@Energy -11.14 kcal/mol@LB@Hybridization Energy -12.1838 kcal/mol@LB@Unfolding Energy - mRNA 0.966795 kcal/mol@LB@Unfolding Energy - sRNA 1.40592 kcal/mol@LB@@LB@Position - mRNA 76 -- 136@LB@Position - sRNA 99 -- 167@LB@Position Seed - mRNA 91 -- 97@LB@Position Seed - sRNA 156 -- 162@LB@ |
