| 151 |
0.00683 |
0.1741 |
bsu18660 |
yoaM |
-13.14 |
0.080203 |
141 -- 183 |
39 -- 78 |
Evidence 4: Homologs of previously reported genes of unknown function |
bsu18880 bsu20490 |
_____________140________________________________184\n_____________|__________________________________|\n5'-UUU...UAAAU_______________CAAA__A____AAAA____UAAAA...AUA-3'\n______________GAACGAUCGU_CCCA____CU_CUUA____CCCC\n______________:|||:|||||_||||____||_||||____||||\n______________UUUGUUAGCA_GGGU____GA_GAAU____GGGG\n3'-AUU...AACCG__________U____A__________AA______UAAAG...CAA-5'\n_____________|__________________________________|\n_____________79_________________________________38\n |
154 -- 160 |
57 -- 63 |
-25.2857 kcal/mol |
3.90608 kcal/mol |
11.0213 kcal/mol |
140 184@LB@ | |@LB@5'-UUU...UAAAU CAAA A AAAA UAAAA...AUA-3'@LB@ GAACGAUCGU CCCA CU CUUA CCCC@LB@ :|||:||||| |||| || |||| ||||@LB@ UUUGUUAGCA GGGU GA GAAU GGGG@LB@3'-AUU...AACCG U A AA UAAAG...CAA-5'@LB@ | |@LB@ 79 38@LB@@LB@@LB@Energy -13.14 kcal/mol@LB@Hybridization Energy -25.2857 kcal/mol@LB@Unfolding Energy - mRNA 3.90608 kcal/mol@LB@Unfolding Energy - sRNA 11.0213 kcal/mol@LB@@LB@Position - mRNA 141 -- 183@LB@Position - sRNA 39 -- 78@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 57 -- 63@LB@ |
| 152 |
0.006831 |
0.1741 |
bsu09480 |
yhdI |
-9.89 |
0.327778 |
76 -- 101 |
102 -- 130 |
GntR family transcriptional regulator |
bsu05240 |
_____________75__________________________102\n_____________|___________________________|\n5'-UCU...UAAUU___________A________C______AUCCC...CAA-3'\n______________GUUUCAUAUGG_____UCAU_UUCUCA\n______________|||:|||||::_____|||:_||||||\n______________CAAGGUAUAUU_____AGUG_AAGAGU\n3'-AUU...UCGUU___________ACAAC____A______AGAAG...CAA-5'\n_____________|___________________________|\n_____________131_________________________101\n |
79 -- 85 |
121 -- 127 |
-18.1477 kcal/mol |
2.87073 kcal/mol |
6.25825 kcal/mol |
75 102@LB@ | |@LB@5'-UCU...UAAUU A C AUCCC...CAA-3'@LB@ GUUUCAUAUGG UCAU UUCUCA@LB@ |||:|||||:: |||: ||||||@LB@ CAAGGUAUAUU AGUG AAGAGU@LB@3'-AUU...UCGUU ACAAC A AGAAG...CAA-5'@LB@ | |@LB@ 131 101@LB@@LB@@LB@Energy -9.89 kcal/mol@LB@Hybridization Energy -18.1477 kcal/mol@LB@Unfolding Energy - mRNA 2.87073 kcal/mol@LB@Unfolding Energy - sRNA 6.25825 kcal/mol@LB@@LB@Position - mRNA 76 -- 101@LB@Position - sRNA 102 -- 130@LB@Position Seed - mRNA 79 -- 85@LB@Position Seed - sRNA 121 -- 127@LB@ |
| 153 |
0.007014 |
0.1751 |
bsu10400 |
yhxC |
-16.66 |
0.014878 |
138 -- 167 |
138 -- 167 |
oxidoreductase |
|
_____________137____________________________168\n_____________|______________________________|\n5'-UCC...UCUAU________AC______AGCA__________AUAAC...CGA-3'\n______________CUUUUCAU__AUAGGA____GGCAUUGUUC\n______________||:|||||__|:||||____|:||||||:|\n______________GAGAAGUA__UGUCCU____CUGUAACAGG\n3'-AUU...AAAAU________GU______GUAA__________CGUCG...CAA-5'\n_____________|______________________________|\n_____________168____________________________137\n |
161 -- 167 |
138 -- 144 |
-29.0079 kcal/mol |
5.02609 kcal/mol |
7.37082 kcal/mol |
137 168@LB@ | |@LB@5'-UCC...UCUAU AC AGCA AUAAC...CGA-3'@LB@ CUUUUCAU AUAGGA GGCAUUGUUC@LB@ ||:||||| |:|||| |:||||||:|@LB@ GAGAAGUA UGUCCU CUGUAACAGG@LB@3'-AUU...AAAAU GU GUAA CGUCG...CAA-5'@LB@ | |@LB@ 168 137@LB@@LB@@LB@Energy -16.66 kcal/mol@LB@Hybridization Energy -29.0079 kcal/mol@LB@Unfolding Energy - mRNA 5.02609 kcal/mol@LB@Unfolding Energy - sRNA 7.37082 kcal/mol@LB@@LB@Position - mRNA 138 -- 167@LB@Position - sRNA 138 -- 167@LB@Position Seed - mRNA 161 -- 167@LB@Position Seed - sRNA 138 -- 144@LB@ |
| 154 |
0.00707 |
0.1751 |
bsu07830 |
yfkO |
-12.90 |
0.089530 |
138 -- 152 |
158 -- 172 |
NAD(P)H nitroreductase YfkO |
|
_____________137____________153\n_____________|______________|\n5'-GGA...GUACU______________UAAUG...AAU-3'\n______________UUUACUUUUUAUCA\n______________||||||:||:||||\n______________AAAUGAGAAGUAGU\n3'-AUU...AAAAA______________UGUCC...CAA-5'\n_____________|______________|\n_____________173____________157\n |
146 -- 152 |
158 -- 164 |
-13.59 kcal/mol |
1.38023 kcal/mol |
1.02713 kcal/mol |
137 153@LB@ | |@LB@5'-GGA...GUACU UAAUG...AAU-3'@LB@ UUUACUUUUUAUCA@LB@ ||||||:||:||||@LB@ AAAUGAGAAGUAGU@LB@3'-AUU...AAAAA UGUCC...CAA-5'@LB@ | |@LB@ 173 157@LB@@LB@@LB@Energy -12.90 kcal/mol@LB@Hybridization Energy -13.59 kcal/mol@LB@Unfolding Energy - mRNA 1.38023 kcal/mol@LB@Unfolding Energy - sRNA 1.02713 kcal/mol@LB@@LB@Position - mRNA 138 -- 152@LB@Position - sRNA 158 -- 172@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 158 -- 164@LB@ |
| 155 |
0.00708 |
0.1751 |
bsu17280 |
ymaD |
-7.93 |
0.631070 |
192 -- 223 |
42 -- 69 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 16267290; Product type pe : enzyme' |
|
_____________191______________________________224\n_____________|________________________________|\n5'-AAA...AAGGA___A_______G_CAGA_____AU________AAAAG...UUC-3'\n______________GGC_AAACAAU_G____UCAUC__UUUUAUUU\n______________|||_|||||||_|____:||||__|:|||||:\n______________CCG_UUUGUUA_C____GGUAG__AGAAUAAG\n3'-AUU...UAGAA___________G_AUG________________GGGUA...CAA-5'\n_____________|________________________________|\n_____________70_______________________________41\n |
217 -- 223 |
42 -- 48 |
-20.5024 kcal/mol |
4.54925 kcal/mol |
8.89481 kcal/mol |
191 224@LB@ | |@LB@5'-AAA...AAGGA A G CAGA AU AAAAG...UUC-3'@LB@ GGC AAACAAU G UCAUC UUUUAUUU@LB@ ||| ||||||| | :|||| |:|||||:@LB@ CCG UUUGUUA C GGUAG AGAAUAAG@LB@3'-AUU...UAGAA G AUG GGGUA...CAA-5'@LB@ | |@LB@ 70 41@LB@@LB@@LB@Energy -7.93 kcal/mol@LB@Hybridization Energy -20.5024 kcal/mol@LB@Unfolding Energy - mRNA 4.54925 kcal/mol@LB@Unfolding Energy - sRNA 8.89481 kcal/mol@LB@@LB@Position - mRNA 192 -- 223@LB@Position - sRNA 42 -- 69@LB@Position Seed - mRNA 217 -- 223@LB@Position Seed - sRNA 42 -- 48@LB@ |
| 156 |
0.007194 |
0.1751 |
bsu12900 |
htrA |
-15.16 |
0.030869 |
26 -- 118 |
38 -- 130 |
serine protease Do-like HtrA |
|
_____________25_______________________________________________________________________________________________________119\n_____________|________________________________________________________________________________________________________|\n5'-CUG...AAUCU_______CGACUCA__________A___A_____U________GU__AC____UUCC___C____UC_________U_______AA____UUCAUUUUUA____ACAGU...AUG-3'\n______________GUUCCAU________GUC_CUUUC_UAU___CAA____AUGAA__GU__CGUU____GCA_UUUU_____ACAAUU___CCCAU__UCUU__________UCCC\n______________|||||||________|||_|||||_:||___|||____|||||__|:__||||____|||_|:||_____|||||:___|||||__||||__________||||\n______________CAAGGUA________CAG_GAAAG_GUA___GUU____UACUU__CG__GCAA____CGU_AGAA_____UGUUAG___GGGUA__AGAA__________AGGG\n3'-AUU...UCGUU_______UAUUACAA___U_____A___GAA___UCAU_____AC__AA___________U____CCGUU______CAU_____G_____UA____________GUAAA...CAA-5'\n_____________|________________________________________________________________________________________________________|\n_____________131______________________________________________________________________________________________________37\n |
26 -- 32 |
124 -- 130 |
-40.6941 kcal/mol |
8.96571 kcal/mol |
22.8172 kcal/mol |
25 119@LB@ | |@LB@5'-CUG...AAUCU CGACUCA A A U GU AC UUCC C UC U AA UUCAUUUUUA ACAGU...AUG-3'@LB@ GUUCCAU GUC CUUUC UAU CAA AUGAA GU CGUU GCA UUUU ACAAUU CCCAU UCUU UCCC@LB@ ||||||| ||| ||||| :|| ||| ||||| |: |||| ||| |:|| |||||: ||||| |||| ||||@LB@ CAAGGUA CAG GAAAG GUA GUU UACUU CG GCAA CGU AGAA UGUUAG GGGUA AGAA AGGG@LB@3'-AUU...UCGUU UAUUACAA U A GAA UCAU AC AA U CCGUU CAU G UA GUAAA...CAA-5'@LB@ | |@LB@ 131 37@LB@@LB@@LB@Energy -15.16 kcal/mol@LB@Hybridization Energy -40.6941 kcal/mol@LB@Unfolding Energy - mRNA 8.96571 kcal/mol@LB@Unfolding Energy - sRNA 22.8172 kcal/mol@LB@@LB@Position - mRNA 26 -- 118@LB@Position - sRNA 38 -- 130@LB@Position Seed - mRNA 26 -- 32@LB@Position Seed - sRNA 124 -- 130@LB@ |
| 157 |
0.007221 |
0.1751 |
bsu40660 |
yybF |
-12.55 |
0.105157 |
243 -- 270 |
125 -- 152 |
MFS transporter |
|
_____________242__________________________271\n_____________|____________________________|\n5'-UGU...AAAUU_____U__U__C_____U__________AUACA...AGC-3'\n______________ACGUU_GC_UU_UUCGC_GCGGGUUUCA\n______________||:||_:|_||_|:|||_||::|||:||\n______________UGUAA_UG_AA_AGGCG_CGUUCAAGGU\n3'-AUU...UGUCC_____C__U__C_____U__________AUAUU...CAA-5'\n_____________|____________________________|\n_____________153__________________________124\n |
264 -- 270 |
125 -- 131 |
-24.436 kcal/mol |
4.92439 kcal/mol |
8.98549 kcal/mol |
242 271@LB@ | |@LB@5'-UGU...AAAUU U U C U AUACA...AGC-3'@LB@ ACGUU GC UU UUCGC GCGGGUUUCA@LB@ ||:|| :| || |:||| ||::|||:||@LB@ UGUAA UG AA AGGCG CGUUCAAGGU@LB@3'-AUU...UGUCC C U C U AUAUU...CAA-5'@LB@ | |@LB@ 153 124@LB@@LB@@LB@Energy -12.55 kcal/mol@LB@Hybridization Energy -24.436 kcal/mol@LB@Unfolding Energy - mRNA 4.92439 kcal/mol@LB@Unfolding Energy - sRNA 8.98549 kcal/mol@LB@@LB@Position - mRNA 243 -- 270@LB@Position - sRNA 125 -- 152@LB@Position Seed - mRNA 264 -- 270@LB@Position Seed - sRNA 125 -- 131@LB@ |
| 158 |
0.007267 |
0.1751 |
bsu33000 |
htrB |
-17.04 |
0.012352 |
105 -- 237 |
50 -- 180 |
serine protease Do-like HtrB |
|
_____________104_____________________________238\n_____________|_______________________________|\n5'-UUU...GUGCU_______UCUCC______U______A_____ACUUG...CUG-3'\n______________UUGCUUU_____UCAUUA_UGGGAC_AUUGA\n______________||:||:|_____||||:|_::||||_|||||\n______________AAUGAGA_____AGUAGU_GUCCUG_UAACU\n3'-AUU...AAAAA__________________U____________GUAAC...CAA-5'\n_____________|_______________________________|\n_____________181_____________________________49\n |
110 -- 116 |
164 -- 170 |
-21.2082 kcal/mol |
7.59468 kcal/mol |
7.14638 kcal/mol |
104 238@LB@ | |@LB@5'-UUU...GUGCU UCUCC U A ACUUG...CUG-3'@LB@ UUGCUUU UCAUUA UGGGAC AUUGA@LB@ ||:||:| ||||:| ::|||| |||||@LB@ AAUGAGA AGUAGU GUCCUG UAACU@LB@3'-AUU...AAAAA U GUAAC...CAA-5'@LB@ | |@LB@ 181 49@LB@@LB@@LB@Energy -17.04 kcal/mol@LB@Hybridization Energy -21.2082 kcal/mol@LB@Unfolding Energy - mRNA 7.59468 kcal/mol@LB@Unfolding Energy - sRNA 7.14638 kcal/mol@LB@@LB@Position - mRNA 105 -- 237@LB@Position - sRNA 50 -- 180@LB@Position Seed - mRNA 110 -- 116@LB@Position Seed - sRNA 164 -- 170@LB@ |
| 159 |
0.007366 |
0.1751 |
bsu06380 |
yebC |
-16.67 |
0.014812 |
77 -- 156 |
105 -- 185 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________76__________________________________________________________________________157\n_____________|___________________________________________________________________________|\n5'-ACC...CAUGC_______AA___G__AAU________________AGGAA___UUC___A__U____UAA________________GCUGA...CGA-3'\n______________UUUACUU___AU_GA___GAC_UUGAC__UGUUC_____GGC___GGU_CC_UAUA___GUGUU_UUAUUUUCUU\n______________||||||:___||_:|___|||_|||||__|||:|_____:||___:||_||_||||___:||||_|:|:|||||:\n______________AAAUGAG___UA_UU___CUG_AACUG__ACAGG_____UCG___UCA_GG_AUAU___UACAA_AGUGAAAGAG\n3'-AUU...AAAAA_______AAG__G__GUC___U_____UA_____CG______U_____A__U____________C__________UAGAA...CAA-5'\n_____________|___________________________________________________________________________|\n_____________186_________________________________________________________________________104\n |
87 -- 93 |
165 -- 171 |
-36.2834 kcal/mol |
12.0661 kcal/mol |
14.3768 kcal/mol |
76 157@LB@ | |@LB@5'-ACC...CAUGC AA G AAU AGGAA UUC A U UAA GCUGA...CGA-3'@LB@ UUUACUU AU GA GAC UUGAC UGUUC GGC GGU CC UAUA GUGUU UUAUUUUCUU@LB@ ||||||: || :| ||| ||||| |||:| :|| :|| || |||| :|||| |:|:|||||:@LB@ AAAUGAG UA UU CUG AACUG ACAGG UCG UCA GG AUAU UACAA AGUGAAAGAG@LB@3'-AUU...AAAAA AAG G GUC U UA CG U A U C UAGAA...CAA-5'@LB@ | |@LB@ 186 104@LB@@LB@@LB@Energy -16.67 kcal/mol@LB@Hybridization Energy -36.2834 kcal/mol@LB@Unfolding Energy - mRNA 12.0661 kcal/mol@LB@Unfolding Energy - sRNA 14.3768 kcal/mol@LB@@LB@Position - mRNA 77 -- 156@LB@Position - sRNA 105 -- 185@LB@Position Seed - mRNA 87 -- 93@LB@Position Seed - sRNA 165 -- 171@LB@ |
| 160 |
0.007403 |
0.1751 |
bsu11139 |
yizC |
-17.39 |
0.010400 |
11 -- 81 |
42 -- 107 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________10____________________________________________________________________________82\n_____________|_____________________________________________________________________________|\n5'-UUG...GACGA_______CG________UG______GG____________CAAAAUG_________AGCAA_____GAAGG_______AGCAG...UGA-3'\n______________CUCAUCU__UCAAAGUA__GAA_GG_______GCAGUCU_______GGUAAACGA_____AUCCA_____UUUAUUC\n______________|||||||__||||||||__|||_||_______|||:|||_______||:||||:|_____|:|||_____:||||||\n______________GAGUAGA__AGUUUCAU__CUU_CC_______CGUUAGA_______CCGUUUGUU_____UGGGU_____GAAUAAG\n3'-AUU...UGAAA_________________UA___A__GAAGCAA_______A_______________AGCA______AGA_________GGGUA...CAA-5'\n_____________|_____________________________________________________________________________|\n_____________108___________________________________________________________________________41\n |
75 -- 81 |
42 -- 48 |
-46.4368 kcal/mol |
15.8344 kcal/mol |
18.2654 kcal/mol |
10 82@LB@ | |@LB@5'-UUG...GACGA CG UG GG CAAAAUG AGCAA GAAGG AGCAG...UGA-3'@LB@ CUCAUCU UCAAAGUA GAA GG GCAGUCU GGUAAACGA AUCCA UUUAUUC@LB@ ||||||| |||||||| ||| || |||:||| ||:||||:| |:||| :||||||@LB@ GAGUAGA AGUUUCAU CUU CC CGUUAGA CCGUUUGUU UGGGU GAAUAAG@LB@3'-AUU...UGAAA UA A GAAGCAA A AGCA AGA GGGUA...CAA-5'@LB@ | |@LB@ 108 41@LB@@LB@@LB@Energy -17.39 kcal/mol@LB@Hybridization Energy -46.4368 kcal/mol@LB@Unfolding Energy - mRNA 15.8344 kcal/mol@LB@Unfolding Energy - sRNA 18.2654 kcal/mol@LB@@LB@Position - mRNA 11 -- 81@LB@Position - sRNA 42 -- 107@LB@Position Seed - mRNA 75 -- 81@LB@Position Seed - sRNA 42 -- 48@LB@ |
