| 141 |
0.006569 |
0.1741 |
bsu19520 |
yojA |
-12.26 |
0.119762 |
158 -- 180 |
120 -- 146 |
permease YojA |
bsu40070 |
_____________157_________________________________________________________181\n_____________|___________________________________________________________|\n5'-GGU...GCUUU__G________________GAAAAGCGCCAAA_________________AA________AACAC...UUU-3'\n______________CU_UUCAUC__UAGG_CAU_____________ACAUUGUC____GCGAG__UCAUAUAA\n______________||_||||||__:|||_|||_____________||||||||____||:||__:|||||||\n______________GA_AAGUAG__GUCC_GUA_____________UGUAACAG____CGUUC__GGUAUAUU\n3'-AUU...AAAAU__G______UU____U___AC___________________GCGU_____AA________ACAAC...CAA-5'\n_____________|___________________________________________________________|\n_____________147_________________________________________________________119\n |
174 -- 180 |
120 -- 126 |
-32.1826 kcal/mol |
10.1468 kcal/mol |
10.5645 kcal/mol |
157 181@LB@ | |@LB@5'-GGU...GCUUU G GAAAAGCGCCAAA AA AACAC...UUU-3'@LB@ CU UUCAUC UAGG CAU ACAUUGUC GCGAG UCAUAUAA@LB@ || |||||| :||| ||| |||||||| ||:|| :|||||||@LB@ GA AAGUAG GUCC GUA UGUAACAG CGUUC GGUAUAUU@LB@3'-AUU...AAAAU G UU U AC GCGU AA ACAAC...CAA-5'@LB@ | |@LB@ 147 119@LB@@LB@@LB@Energy -12.26 kcal/mol@LB@Hybridization Energy -32.1826 kcal/mol@LB@Unfolding Energy - mRNA 10.1468 kcal/mol@LB@Unfolding Energy - sRNA 10.5645 kcal/mol@LB@@LB@Position - mRNA 158 -- 180@LB@Position - sRNA 120 -- 146@LB@Position Seed - mRNA 174 -- 180@LB@Position Seed - sRNA 120 -- 126@LB@ |
| 142 |
0.006636 |
0.1741 |
bsu22510 |
ypjC |
-12.74 |
0.096342 |
122 -- 155 |
156 -- 190 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________121_____________________________________________________________________________156\n_____________|_______________________________________________________________________________|\n5'-UAA...UCAUC_____A___GUUAGCUAAUU___AU___U______AA_____A_____C_____________C____UU__________CACUA...UGG-3'\n______________CUCUU_CAU___________CAG__ACG_UUGACA__UGUUU__AGCA_GUU________UG_UGUU__CUUUUUCAUC\n______________|||||_|||___________|||__||:_||||||__|||::__||||_|||________||_|||:__||||:|||||\n______________GAGAA_GUA___________GUC__UGU_AACUGU__ACAGG__UCGU_CAA________AC_ACAG__GAAAGAGUAG\n3'-AUU...AAAAU_________GUU___________C___________A______CG____U___GGUAUAUU__A____U___________AAGUU...CAA-5'\n_____________|_______________________________________________________________________________|\n_____________191_____________________________________________________________________________155\n |
146 -- 152 |
102 -- 108 |
-35.2093 kcal/mol |
10.3676 kcal/mol |
14.8184 kcal/mol |
121 156@LB@ | |@LB@5'-UAA...UCAUC A GUUAGCUAAUU AU U AA A C C UU CACUA...UGG-3'@LB@ CUCUU CAU CAG ACG UUGACA UGUUU AGCA GUU UG UGUU CUUUUUCAUC@LB@ ||||| ||| ||| ||: |||||| |||:: |||| ||| || |||: ||||:|||||@LB@ GAGAA GUA GUC UGU AACUGU ACAGG UCGU CAA AC ACAG GAAAGAGUAG@LB@3'-AUU...AAAAU GUU C A CG U GGUAUAUU A U AAGUU...CAA-5'@LB@ | |@LB@ 191 155@LB@@LB@@LB@Energy -12.74 kcal/mol@LB@Hybridization Energy -35.2093 kcal/mol@LB@Unfolding Energy - mRNA 10.3676 kcal/mol@LB@Unfolding Energy - sRNA 14.8184 kcal/mol@LB@@LB@Position - mRNA 122 -- 155@LB@Position - sRNA 156 -- 190@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 102 -- 108@LB@ |
| 143 |
0.006663 |
0.1741 |
bsu05790 |
ydhK |
-18.88 |
0.004963 |
179 -- 222 |
125 -- 163 |
Evidence 5: No homology to any previously reported sequences |
|
_____________178__________________________________________223\n_____________|____________________________________________|\n5'-GUG...UAGUA_______G_______GUUUU____________G__AA___UA__GGAAA...UUU-3'\n______________UUAUCGA_AGGGCAU_____CAUUGUCUGCGG_CA__AGU__CA\n______________|:|||:|_|||:|||_____|||||||:||:|_||__|||__||\n______________AGUAGUU_UCCUGUA_____GUAACAGGCGUC_GU__UCA__GU\n3'-AUU...UGAGA_______G_______ACU______________________AG__AUAUU...CAA-5'\n_____________|____________________________________________|\n_____________164__________________________________________124\n |
204 -- 210 |
134 -- 140 |
-40.9125 kcal/mol |
11.6904 kcal/mol |
10.3584 kcal/mol |
178 223@LB@ | |@LB@5'-GUG...UAGUA G GUUUU G AA UA GGAAA...UUU-3'@LB@ UUAUCGA AGGGCAU CAUUGUCUGCGG CA AGU CA@LB@ |:|||:| |||:||| |||||||:||:| || ||| ||@LB@ AGUAGUU UCCUGUA GUAACAGGCGUC GU UCA GU@LB@3'-AUU...UGAGA G ACU AG AUAUU...CAA-5'@LB@ | |@LB@ 164 124@LB@@LB@@LB@Energy -18.88 kcal/mol@LB@Hybridization Energy -40.9125 kcal/mol@LB@Unfolding Energy - mRNA 11.6904 kcal/mol@LB@Unfolding Energy - sRNA 10.3584 kcal/mol@LB@@LB@Position - mRNA 179 -- 222@LB@Position - sRNA 125 -- 163@LB@Position Seed - mRNA 204 -- 210@LB@Position Seed - sRNA 134 -- 140@LB@ |
| 144 |
0.006712 |
0.1741 |
bsu25040 |
yqgB |
-19.28 |
0.004052 |
60 -- 160 |
55 -- 168 |
Evidence 2a: Function of homologous gene experimentally demonstrated in an other organism; PubMedId: 15294815; Product type ph: phenotype |
|
_____________59_____________________________________________________161\n_____________|______________________________________________________|\n5'-UAA...AAAAC__C_______AACA__CAAU______A_______AG___CCAUCC_________ACUCU...UUU-3'\n______________CG_UGCAAUC____GC____ACAA_C_UAUCCAU__CUU______UCCCAUUUU\n______________||_|||||||____||____||||_|_||:||||__|||______:|||||||:\n______________GC_ACGUUAG____CG____UGUU_G_AUGGGUA__GAA______GGGGUAAAG\n3'-AUU...CCGAA__A_______AAC___UU______A_C_______GA___UAA____________AAACU...CAA-5'\n_____________|______________________________________________________|\n_____________169____________________________________________________54\n |
105 -- 111 |
34 -- 40 |
-33.5165 kcal/mol |
5.00067 kcal/mol |
13.93 kcal/mol |
59 161@LB@ | |@LB@5'-UAA...AAAAC C AACA CAAU A AG CCAUCC ACUCU...UUU-3'@LB@ CG UGCAAUC GC ACAA C UAUCCAU CUU UCCCAUUUU@LB@ || ||||||| || |||| | ||:|||| ||| :|||||||:@LB@ GC ACGUUAG CG UGUU G AUGGGUA GAA GGGGUAAAG@LB@3'-AUU...CCGAA A AAC UU A C GA UAA AAACU...CAA-5'@LB@ | |@LB@ 169 54@LB@@LB@@LB@Energy -19.28 kcal/mol@LB@Hybridization Energy -33.5165 kcal/mol@LB@Unfolding Energy - mRNA 5.00067 kcal/mol@LB@Unfolding Energy - sRNA 13.93 kcal/mol@LB@@LB@Position - mRNA 60 -- 160@LB@Position - sRNA 55 -- 168@LB@Position Seed - mRNA 105 -- 111@LB@Position Seed - sRNA 34 -- 40@LB@ |
| 145 |
0.006739 |
0.1741 |
bsu12460 |
xlyB |
-11.80 |
0.146979 |
21 -- 64 |
155 -- 186 |
N-acetylmuramoyl-L-alanine amidase XlyB |
bsu12810 bsu25710 bsu25900 bsu21410 |
_____________20____________________________________65\n_____________|_____________________________________|\n5'-UCC...CCACA_____C_G_____AUUUCUC____CU_______A___UUAAA...CGG-3'\n______________UGCUC_U_AUUAA_______CGUU__CAUUGUC_GCA\n______________|:|||_|_||:||_______|:||__|||||||_|||\n______________AUGAG_A_UAGUU_______GUAA__GUAACAG_CGU\n3'-AUU...AAAAA_____A_G_____GUCCU______CU_______G___CGUUC...CAA-5'\n_____________|_____________________________________|\n_____________187___________________________________154\n |
54 -- 60 |
139 -- 145 |
-22.5299 kcal/mol |
4.58402 kcal/mol |
8.54622 kcal/mol |
20 65@LB@ | |@LB@5'-UCC...CCACA C G AUUUCUC CU A UUAAA...CGG-3'@LB@ UGCUC U AUUAA CGUU CAUUGUC GCA@LB@ |:||| | ||:|| |:|| ||||||| |||@LB@ AUGAG A UAGUU GUAA GUAACAG CGU@LB@3'-AUU...AAAAA A G GUCCU CU G CGUUC...CAA-5'@LB@ | |@LB@ 187 154@LB@@LB@@LB@Energy -11.80 kcal/mol@LB@Hybridization Energy -22.5299 kcal/mol@LB@Unfolding Energy - mRNA 4.58402 kcal/mol@LB@Unfolding Energy - sRNA 8.54622 kcal/mol@LB@@LB@Position - mRNA 21 -- 64@LB@Position - sRNA 155 -- 186@LB@Position Seed - mRNA 54 -- 60@LB@Position Seed - sRNA 139 -- 145@LB@ |
| 146 |
0.00677 |
0.1741 |
bsu29690 |
acuA |
-9.85 |
0.331997 |
126 -- 137 |
14 -- 25 |
acetoin utilization protein AcuA |
|
_____________125____________138\n_____________|______________|\n5'-UAU...AUUCA______________UUUUA...AUG-3'\n______________UAUUCUUC_UCAAU\n______________||:|||||_||||:\n______________AUGAGAAG_AGUUG\n3'-AUU...AAAAA________U_____UCCUG...CAA-5'\n_____________|______________|\n_____________26_____________13\n |
95 -- 101 |
162 -- 168 |
-12.3213 kcal/mol |
1.49895 kcal/mol |
1.40683 kcal/mol |
125 138@LB@ | |@LB@5'-UAU...AUUCA UUUUA...AUG-3'@LB@ UAUUCUUC UCAAU@LB@ ||:||||| ||||:@LB@ AUGAGAAG AGUUG@LB@3'-AUU...AAAAA U UCCUG...CAA-5'@LB@ | |@LB@ 26 13@LB@@LB@@LB@Energy -9.85 kcal/mol@LB@Hybridization Energy -12.3213 kcal/mol@LB@Unfolding Energy - mRNA 1.49895 kcal/mol@LB@Unfolding Energy - sRNA 1.40683 kcal/mol@LB@@LB@Position - mRNA 126 -- 137@LB@Position - sRNA 14 -- 25@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 162 -- 168@LB@ |
| 147 |
0.006774 |
0.1741 |
bsu08550 |
sspK |
-14.99 |
0.033457 |
165 -- 208 |
137 -- 183 |
small acid-soluble spore protein K |
|
_____________164_____________________________209\n_____________|_______________________________|\n5'-UUU...UUGAU___GAACC___G_____________G_____AAAUA...ACG-3'\n______________CUC_____UAG_GGGAUAU_GACAU_GUCCG\n______________|||_____:|:_:|||:||_|||||_|||||\n______________GAG_____GUU_UCCUGUA_CUGUA_CAGGC\n3'-AUU...AAAAU___AAGUA___G_______A_____A_____GUCGU...CAA-5'\n_____________|_______________________________|\n_____________184_____________________________136\n |
191 -- 197 |
149 -- 155 |
-28.8408 kcal/mol |
8.70873 kcal/mol |
7.49334 kcal/mol |
164 209@LB@ | |@LB@5'-UUU...UUGAU GAACC G G AAAUA...ACG-3'@LB@ CUC UAG GGGAUAU GACAU GUCCG@LB@ ||| :|: :|||:|| ||||| |||||@LB@ GAG GUU UCCUGUA CUGUA CAGGC@LB@3'-AUU...AAAAU AAGUA G A A GUCGU...CAA-5'@LB@ | |@LB@ 184 136@LB@@LB@@LB@Energy -14.99 kcal/mol@LB@Hybridization Energy -28.8408 kcal/mol@LB@Unfolding Energy - mRNA 8.70873 kcal/mol@LB@Unfolding Energy - sRNA 7.49334 kcal/mol@LB@@LB@Position - mRNA 165 -- 208@LB@Position - sRNA 137 -- 183@LB@Position Seed - mRNA 191 -- 197@LB@Position Seed - sRNA 149 -- 155@LB@ |
| 148 |
0.006782 |
0.1741 |
bsu14050 |
ykuE |
-11.57 |
0.163142 |
1 -- 16 |
37 -- 54 |
metallophosphoesterase |
|
______________1__________________________17\n______________|__________________________|\n___________5'-_____G____________GC_GC____CCCGC...AUC-3'\n______________CCAUC_CU__UUUCCCAU__C__UUGA\n______________|||||_||__||:|||||__|__||||\n______________GGUAG_GA__AAGGGGUA__G__AACU\n3'-AUU...GCAUG_____A__AU________AA_A_____UUAAA...CAA-5'\n_____________|___________________________|\n_____________55__________________________36\n |
10 -- 16 |
37 -- 43 |
-20.8225 kcal/mol |
1.63511 kcal/mol |
9.4766 kcal/mol |
1 17@LB@ | |@LB@ 5'- G GC GC CCCGC...AUC-3'@LB@ CCAUC CU UUUCCCAU C UUGA@LB@ ||||| || ||:||||| | ||||@LB@ GGUAG GA AAGGGGUA G AACU@LB@3'-AUU...GCAUG A AU AA A UUAAA...CAA-5'@LB@ | |@LB@ 55 36@LB@@LB@@LB@Energy -11.57 kcal/mol@LB@Hybridization Energy -20.8225 kcal/mol@LB@Unfolding Energy - mRNA 1.63511 kcal/mol@LB@Unfolding Energy - sRNA 9.4766 kcal/mol@LB@@LB@Position - mRNA 1 -- 16@LB@Position - sRNA 37 -- 54@LB@Position Seed - mRNA 10 -- 16@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 149 |
0.006788 |
0.1741 |
bsu28960 |
ytxC |
-13.61 |
0.064351 |
236 -- 300 |
132 -- 186 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________235________________________301\n_____________|__________________________|\n________5'-CGA__U________U______________CUUUC...GCA-3'\n______________GC__UUUUGGC_GGCAAUC_UGCCCG\n______________||__|:|||||_::|||||_|:|||:\n______________CG__AGAACCG_UUGUUAG_AUGGGU\n3'-AUU...AGCAA__UU_______U_______C______AGAGA...CAA-5'\n_____________|__________________________|\n_____________187________________________131\n |
7 -- 13 |
67 -- 73 |
-27.0931 kcal/mol |
7.22978 kcal/mol |
8.4393 kcal/mol |
235 301@LB@ | |@LB@ 5'-CGA U U CUUUC...GCA-3'@LB@ GC UUUUGGC GGCAAUC UGCCCG@LB@ || |:||||| ::||||| |:|||:@LB@ CG AGAACCG UUGUUAG AUGGGU@LB@3'-AUU...AGCAA UU U C AGAGA...CAA-5'@LB@ | |@LB@ 187 131@LB@@LB@@LB@Energy -13.61 kcal/mol@LB@Hybridization Energy -27.0931 kcal/mol@LB@Unfolding Energy - mRNA 7.22978 kcal/mol@LB@Unfolding Energy - sRNA 8.4393 kcal/mol@LB@@LB@Position - mRNA 236 -- 300@LB@Position - sRNA 132 -- 186@LB@Position Seed - mRNA 7 -- 13@LB@Position Seed - sRNA 67 -- 73@LB@ |
| 150 |
0.006829 |
0.1741 |
bsu17970 |
cotM |
-16.22 |
0.018442 |
252 -- 285 |
70 -- 101 |
spore coat protein M |
|
_____________251______________________286\n_____________|________________________|\n5'-AUG...GUAUG________________________UUGAA...UCG-3'\n______________GAUGAAUGGCUUCG__GCAAUUUU\n______________:|||||||||||||__|||||:||\n______________UUACUUACCGAAGC__CGUUAGAA\n3'-AUU...UUUCA______________AA________CCGUU...CAA-5'\n_____________|________________________|\n_____________102______________________69\n |
278 -- 284 |
71 -- 77 |
-28.716 kcal/mol |
7.78723 kcal/mol |
6.67697 kcal/mol |
251 286@LB@ | |@LB@5'-AUG...GUAUG UUGAA...UCG-3'@LB@ GAUGAAUGGCUUCG GCAAUUUU@LB@ :||||||||||||| |||||:||@LB@ UUACUUACCGAAGC CGUUAGAA@LB@3'-AUU...UUUCA AA CCGUU...CAA-5'@LB@ | |@LB@ 102 69@LB@@LB@@LB@Energy -16.22 kcal/mol@LB@Hybridization Energy -28.716 kcal/mol@LB@Unfolding Energy - mRNA 7.78723 kcal/mol@LB@Unfolding Energy - sRNA 6.67697 kcal/mol@LB@@LB@Position - mRNA 252 -- 285@LB@Position - sRNA 70 -- 101@LB@Position Seed - mRNA 278 -- 284@LB@Position Seed - sRNA 71 -- 77@LB@ |
