Input and runtime details for job 3202356 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3202356 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:02 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:03:47 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:03:55 CET 2018
	Job Completed		@ Sat Feb 17 02:04:28 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3202356&toolName=CopraRNA ( 30 days expiry )

Description of the job

GcvB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
135	0.006796	0.1774	stm1044	sodC	-14.39	0.147293	208 -- 244	43 -- 78	Gifsy-2 prophage superoxide dismutase precursor (Cu-Zn)	stm1440 stm0924	_____________207__________245\n_____________\|____________\|\n5'-GAA...GCUUU____________CAGGG...CCA-3'\n______________GGUGGUCUCGCU\n______________\|\|\|:\|\|\|\|\|\|\|\|\n______________CCAUCAGAGCGA\n3'-UUU...UUUUC____________UGGCA...UCA-5'\n_____________\|____________\|\n_____________79___________42\n	78 -- 84	117 -- 123	-21.9622 kcal/mol	4.44224 kcal/mol	6.55795 kcal/mol	207 245@LB@ \| \|@LB@5'-GAA...GCUUU CAGGG...CCA-3'@LB@ GGUGGUCUCGCU@LB@ \|\|\|:\|\|\|\|\|\|\|\|@LB@ CCAUCAGAGCGA@LB@3'-UUU...UUUUC UGGCA...UCA-5'@LB@ \| \|@LB@ 79 42@LB@@LB@@LB@Energy -14.39 kcal/mol@LB@Hybridization Energy -21.9622 kcal/mol@LB@Unfolding Energy - mRNA 4.44224 kcal/mol@LB@Unfolding Energy - sRNA 6.55795 kcal/mol@LB@@LB@Position - mRNA 208 -- 244@LB@Position - sRNA 43 -- 78@LB@Position Seed - mRNA 78 -- 84@LB@Position Seed - sRNA 117 -- 123@LB@
75	0.003517	0.1596	stm1214	ycfR	-14.36	0.148951	168 -- 212	35 -- 81	multiple stress resistance protein BhsA	stm0823	_____________167______________213\n_____________\|________________\|\n5'-AAA...ACCAC_________CA_____AUUCA...AAC-3'\n______________UUCUGGCUC___GAGU\n______________\|\|\|:\|\|\|\|\|___:\|\|\|\n______________AAGGCCGAG___UUCA\n3'-UUU...AAAGC_________UCC____-5'\n_____________\|_______________\|\n_____________82______________35\n	15 -- 21	8 -- 14	-15.9373 kcal/mol	4.12962 kcal/mol	4.82557 kcal/mol	167 213@LB@ \| \|@LB@5'-AAA...ACCAC CA AUUCA...AAC-3'@LB@ UUCUGGCUC GAGU@LB@ \|\|\|:\|\|\|\|\| :\|\|\|@LB@ AAGGCCGAG UUCA@LB@3'-UUU...AAAGC UCC -5'@LB@ \| \|@LB@ 82 35@LB@@LB@@LB@Energy -14.36 kcal/mol@LB@Hybridization Energy -15.9373 kcal/mol@LB@Unfolding Energy - mRNA 4.12962 kcal/mol@LB@Unfolding Energy - sRNA 4.82557 kcal/mol@LB@@LB@Position - mRNA 168 -- 212@LB@Position - sRNA 35 -- 81@LB@Position Seed - mRNA 15 -- 21@LB@Position Seed - sRNA 8 -- 14@LB@
177	0.009665	0.1945	stm0079	yaaU	-14.30	0.152587	187 -- 218	40 -- 79	MFS transporter		_____________186___________219\n_____________\|_____________\|\n5'-GAA...GUUGA____G________CAUGC...UGC-3'\n______________AACA_GACAUCAC\n______________\|\|\|\|_:\|\|\|\|\|\|\|\n______________UUGU_UUGUAGUG\n3'-UUU...UGUUG____G________UUGGC...UCA-5'\n_____________\|_____________\|\n_____________80____________39\n	193 -- 199	67 -- 73	-15.1412 kcal/mol	3.61479 kcal/mol	3.57243 kcal/mol	186 219@LB@ \| \|@LB@5'-GAA...GUUGA G CAUGC...UGC-3'@LB@ AACA GACAUCAC@LB@ \|\|\|\| :\|\|\|\|\|\|\|@LB@ UUGU UUGUAGUG@LB@3'-UUU...UGUUG G UUGGC...UCA-5'@LB@ \| \|@LB@ 80 39@LB@@LB@@LB@Energy -14.30 kcal/mol@LB@Hybridization Energy -15.1412 kcal/mol@LB@Unfolding Energy - mRNA 3.61479 kcal/mol@LB@Unfolding Energy - sRNA 3.57243 kcal/mol@LB@@LB@Position - mRNA 187 -- 218@LB@Position - sRNA 40 -- 79@LB@Position Seed - mRNA 193 -- 199@LB@Position Seed - sRNA 67 -- 73@LB@
83	0.003855	0.1596	stm1680	ycjI	-14.22	0.156552	151 -- 200	34 -- 86	murein peptide amidase A		_____________150_________201\n_____________\|___________\|\n5'-GAA...CUGAA___________UUCGC...CGG-3'\n______________CAUUAUGGACG\n______________\|\|:\|\|\|\|\|\|\|:\n______________GUGAUACCUGU\n3'-UUU...AAUUA___________CUGUC...UCA-5'\n_____________\|___________\|\n_____________87__________33\n	259 -- 265	159 -- 165	-14.9153 kcal/mol	1.6295 kcal/mol	3.40995 kcal/mol	150 201@LB@ \| \|@LB@5'-GAA...CUGAA UUCGC...CGG-3'@LB@ CAUUAUGGACG@LB@ \|\|:\|\|\|\|\|\|\|:@LB@ GUGAUACCUGU@LB@3'-UUU...AAUUA CUGUC...UCA-5'@LB@ \| \|@LB@ 87 33@LB@@LB@@LB@Energy -14.22 kcal/mol@LB@Hybridization Energy -14.9153 kcal/mol@LB@Unfolding Energy - mRNA 1.6295 kcal/mol@LB@Unfolding Energy - sRNA 3.40995 kcal/mol@LB@@LB@Position - mRNA 151 -- 200@LB@Position - sRNA 34 -- 86@LB@Position Seed - mRNA 259 -- 265@LB@Position Seed - sRNA 159 -- 165@LB@
126	0.006333	0.1774	stm1584	ansP	-14.21	0.157463	122 -- 184	36 -- 93	amino acid permease		_____________121_________________185\n_____________\|___________________\|\n5'-AUA...UGUCA________UAUAACA____ACGUA...GUC-3'\n______________GGACAGGC_______GGUA\n______________\|\|\|\|\|\|:\|_______\|\|\|\|\n______________CCUGUCUG_______CCAU\n3'-UUU...UGAUA________UC_________UUACA...UCA-5'\n_____________\|___________________\|\n_____________94__________________35\n	178 -- 184	156 -- 162	-15.5225 kcal/mol	4.18435 kcal/mol	3.12041 kcal/mol	121 185@LB@ \| \|@LB@5'-AUA...UGUCA UAUAACA ACGUA...GUC-3'@LB@ GGACAGGC GGUA@LB@ \|\|\|\|\|\|:\| \|\|\|\|@LB@ CCUGUCUG CCAU@LB@3'-UUU...UGAUA UC UUACA...UCA-5'@LB@ \| \|@LB@ 94 35@LB@@LB@@LB@Energy -14.21 kcal/mol@LB@Hybridization Energy -15.5225 kcal/mol@LB@Unfolding Energy - mRNA 4.18435 kcal/mol@LB@Unfolding Energy - sRNA 3.12041 kcal/mol@LB@@LB@Position - mRNA 122 -- 184@LB@Position - sRNA 36 -- 93@LB@Position Seed - mRNA 178 -- 184@LB@Position Seed - sRNA 156 -- 162@LB@
178	0.009774	0.1951	stm2667	pheA	-14.20	0.157815	177 -- 228	36 -- 93	chorismate mutase		_____________176__________________229\n_____________\|____________________\|\n5'-GAC...ACAAA___G______C_________GGAAA...UUG-3'\n______________AAA_GCAACA_UAUGACAUC\n______________\|\|\|_:\|\|\|\|\|_:\|::\|\|\|\|\|\n______________UUU_UGUUGU_GUGUUGUAG\n3'-UUU...UAACG___G______U_________UGUUG...UCA-5'\n_____________\|____________________\|\n_____________94___________________35\n	202 -- 208	69 -- 75	-17.8987 kcal/mol	1.45353 kcal/mol	4.11387 kcal/mol	176 229@LB@ \| \|@LB@5'-GAC...ACAAA G C GGAAA...UUG-3'@LB@ AAA GCAACA UAUGACAUC@LB@ \|\|\| :\|\|\|\|\| :\|::\|\|\|\|\|@LB@ UUU UGUUGU GUGUUGUAG@LB@3'-UUU...UAACG G U UGUUG...UCA-5'@LB@ \| \|@LB@ 94 35@LB@@LB@@LB@Energy -14.20 kcal/mol@LB@Hybridization Energy -17.8987 kcal/mol@LB@Unfolding Energy - mRNA 1.45353 kcal/mol@LB@Unfolding Energy - sRNA 4.11387 kcal/mol@LB@@LB@Position - mRNA 177 -- 228@LB@Position - sRNA 36 -- 93@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 69 -- 75@LB@
161	0.008343	0.1845	stm3321	yhbH	-14.15	0.160801	194 -- 246	48 -- 88	ribosomal subunit interface protein		_____________193______________________247\n_____________\|________________________\|\n5'-GAU...AAGGA______UAU_____U_________CGGAC...GGA-3'\n______________AGACAC___GCAGC_CAAUAUCAC\n______________\|:\|\|\|\|___:\|\|:\|_\|\|\|:\|\|\|\|\|\n______________UUUGUG___UGUUG_GUUGUAGUG\n3'-UUU...UAACG______U_______U_________UUGGC...UCA-5'\n_____________\|________________________\|\n_____________89_______________________47\n	211 -- 217	67 -- 73	-22.4132 kcal/mol	2.65406 kcal/mol	5.48747 kcal/mol	193 247@LB@ \| \|@LB@5'-GAU...AAGGA UAU U CGGAC...GGA-3'@LB@ AGACAC GCAGC CAAUAUCAC@LB@ \|:\|\|\|\| :\|\|:\| \|\|\|:\|\|\|\|\|@LB@ UUUGUG UGUUG GUUGUAGUG@LB@3'-UUU...UAACG U U UUGGC...UCA-5'@LB@ \| \|@LB@ 89 47@LB@@LB@@LB@Energy -14.15 kcal/mol@LB@Hybridization Energy -22.4132 kcal/mol@LB@Unfolding Energy - mRNA 2.65406 kcal/mol@LB@Unfolding Energy - sRNA 5.48747 kcal/mol@LB@@LB@Position - mRNA 194 -- 246@LB@Position - sRNA 48 -- 88@LB@Position Seed - mRNA 211 -- 217@LB@Position Seed - sRNA 67 -- 73@LB@
137	0.006825	0.1774	stm1196	acpP	-14.14	0.161333	197 -- 223	36 -- 60	acyl carrier protein		_____________196___________________________________________224\n_____________\|_____________________________________________\|\n5'-ACU...AGAAC_______GAAA_____C____A____CU__GCGUUAA________AAGUA...UGG-3'\n______________GCGUUAA____AUUAU_GGCG_ACAG__GG_______GCAGGAAG\n______________:\|:\|\|\|\|____\|:\|\|\|_\|:\|:_\|\|\|\|__\|\|_______:\|\|\|\|\|\|\|\n______________UGUAAUU____UGAUA_CUGU_UGUC__CC_______UGUCCUUC\n3'-UUU...CACGA_______AG_______C____C________AUUUACA________ACUUU...UCA-5'\n_____________\|_____________________________________________\|\n_____________61____________________________________________35\n	258 -- 264	137 -- 143	-28.6095 kcal/mol	10.3633 kcal/mol	7.13805 kcal/mol	196 224@LB@ \| \|@LB@5'-ACU...AGAAC GAAA C A CU GCGUUAA AAGUA...UGG-3'@LB@ GCGUUAA AUUAU GGCG ACAG GG GCAGGAAG@LB@ :\|:\|\|\|\| \|:\|\|\| \|:\|: \|\|\|\| \|\| :\|\|\|\|\|\|\|@LB@ UGUAAUU UGAUA CUGU UGUC CC UGUCCUUC@LB@3'-UUU...CACGA AG C C AUUUACA ACUUU...UCA-5'@LB@ \| \|@LB@ 61 35@LB@@LB@@LB@Energy -14.14 kcal/mol@LB@Hybridization Energy -28.6095 kcal/mol@LB@Unfolding Energy - mRNA 10.3633 kcal/mol@LB@Unfolding Energy - sRNA 7.13805 kcal/mol@LB@@LB@Position - mRNA 197 -- 223@LB@Position - sRNA 36 -- 60@LB@Position Seed - mRNA 258 -- 264@LB@Position Seed - sRNA 137 -- 143@LB@
196	0.01089	0.197	stm3904	ilvD	-14.14	0.161583	237 -- 253	179 -- 194	dihydroxy-acid dehydratase		_____________236___________254\n_____________\|_____________\|\n5'-CGA...UGAAG___AA________AGUAA...GCA-3'\n______________GGA__AGAUGGGG\n______________\|\|\|__\|\|\|::\|\|\|\n______________CCU__UCUGUCCC\n3'-UUU...UGAUA___G_________AUUUA...UCA-5'\n_____________\|_____________\|\n_____________195___________178\n	182 -- 188	152 -- 158	-15.6614 kcal/mol	1.17371 kcal/mol	3.10307 kcal/mol	236 254@LB@ \| \|@LB@5'-CGA...UGAAG AA AGUAA...GCA-3'@LB@ GGA AGAUGGGG@LB@ \|\|\| \|\|\|::\|\|\|@LB@ CCU UCUGUCCC@LB@3'-UUU...UGAUA G AUUUA...UCA-5'@LB@ \| \|@LB@ 195 178@LB@@LB@@LB@Energy -14.14 kcal/mol@LB@Hybridization Energy -15.6614 kcal/mol@LB@Unfolding Energy - mRNA 1.17371 kcal/mol@LB@Unfolding Energy - sRNA 3.10307 kcal/mol@LB@@LB@Position - mRNA 237 -- 253@LB@Position - sRNA 179 -- 194@LB@Position Seed - mRNA 182 -- 188@LB@Position Seed - sRNA 152 -- 158@LB@
71	0.003339	0.1596	stm0105	yabI	-14.10	0.163814	151 -- 168	141 -- 162	similar to E. coli orf hypothetical protein (AAC73176.1); Blastp hit to AAC73176.1 (254 aa) 89% identity in aa 1 - 253		_____________150____________________169\n_____________\|______________________\|\n5'-UGA...ACGAA__G___________________AAUCC...GGU-3'\n______________GG_GGACGGGGUAA____GCAG\n______________\|:_:\|\|\|:\|\|\|\|\|\|____:\|\|\|\n______________CU_UCUGUCCCAUU____UGUC\n3'-UUU...GAUAC__G___________UACA____CUUCA...UCA-5'\n_____________\|______________________\|\n_____________163____________________140\n	158 -- 164	149 -- 155	-23.8591 kcal/mol	5.26969 kcal/mol	3.68511 kcal/mol	150 169@LB@ \| \|@LB@5'-UGA...ACGAA G AAUCC...GGU-3'@LB@ GG GGACGGGGUAA GCAG@LB@ \|: :\|\|\|:\|\|\|\|\|\| :\|\|\|@LB@ CU UCUGUCCCAUU UGUC@LB@3'-UUU...GAUAC G UACA CUUCA...UCA-5'@LB@ \| \|@LB@ 163 140@LB@@LB@@LB@Energy -14.10 kcal/mol@LB@Hybridization Energy -23.8591 kcal/mol@LB@Unfolding Energy - mRNA 5.26969 kcal/mol@LB@Unfolding Energy - sRNA 3.68511 kcal/mol@LB@@LB@Position - mRNA 151 -- 168@LB@Position - sRNA 141 -- 162@LB@Position Seed - mRNA 158 -- 164@LB@Position Seed - sRNA 149 -- 155@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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