| 131 |
0.006003 |
0.1741 |
bsu37510 |
pbpG |
-10.90 |
0.216982 |
70 -- 106 |
84 -- 108 |
penicillin-binding protein 2D |
|
_____________69____________________________________107\n_____________|_____________________________________|\n5'-UGU...UGAAA___________CCUAAGGAAA_____UAGA_______GGGGA...UGU-3'\n______________UCUCAUUUUCA__________AAGUA____GGAUGGC\n______________||||||:||||__________|||||____|:|||||\n______________AGAGUAGAAGU__________UUCAU____CUUACCG\n3'-AUU...GUGAA__________________________UA_________AAGCA...CAA-5'\n_____________|_____________________________________|\n_____________109___________________________________83\n |
100 -- 106 |
84 -- 90 |
-24.2284 kcal/mol |
7.40636 kcal/mol |
9.09036 kcal/mol |
69 107@LB@ | |@LB@5'-UGU...UGAAA CCUAAGGAAA UAGA GGGGA...UGU-3'@LB@ UCUCAUUUUCA AAGUA GGAUGGC@LB@ ||||||:|||| ||||| |:|||||@LB@ AGAGUAGAAGU UUCAU CUUACCG@LB@3'-AUU...GUGAA UA AAGCA...CAA-5'@LB@ | |@LB@ 109 83@LB@@LB@@LB@Energy -10.90 kcal/mol@LB@Hybridization Energy -24.2284 kcal/mol@LB@Unfolding Energy - mRNA 7.40636 kcal/mol@LB@Unfolding Energy - sRNA 9.09036 kcal/mol@LB@@LB@Position - mRNA 70 -- 106@LB@Position - sRNA 84 -- 108@LB@Position Seed - mRNA 100 -- 106@LB@Position Seed - sRNA 84 -- 90@LB@ |
| 132 |
0.006133 |
0.1741 |
bsu24800 |
yqgW |
-15.55 |
0.025469 |
1 -- 63 |
31 -- 85 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
______________1_________________________________________________________________64\n______________|_________________________________________________________________|\n___________5'-__G____C_____________CCUACU____U____C________ACUA____CA___C_______UUUAU...CCG-3'\n______________GC_UUCG_UUG_AAUC__GGC______GGCA_UCGU_UCCAUUUC____UUAU__CCU_UUUCUUU\n______________||_||||_|||_||||__|||______::||_||||_:||||:||____||||__||:_|||||||\n______________CG_AAGC_AAC_UUAG__CCG______UUGU_AGCA_GGGUAGAG____AAUA__GGG_AAAGAAA\n3'-AUU...CUUAC___________G____AA___U_________U____U________________AG___U_______CUUUA...CAA-5'\n_____________|__________________________________________________________________|\n_____________86_________________________________________________________________30\n |
36 -- 42 |
48 -- 54 |
-38.6272 kcal/mol |
10.4973 kcal/mol |
14.833 kcal/mol |
1 64@LB@ | |@LB@ 5'- G C CCUACU U C ACUA CA C UUUAU...CCG-3'@LB@ GC UUCG UUG AAUC GGC GGCA UCGU UCCAUUUC UUAU CCU UUUCUUU@LB@ || |||| ||| |||| ||| ::|| |||| :||||:|| |||| ||: |||||||@LB@ CG AAGC AAC UUAG CCG UUGU AGCA GGGUAGAG AAUA GGG AAAGAAA@LB@3'-AUU...CUUAC G AA U U U AG U CUUUA...CAA-5'@LB@ | |@LB@ 86 30@LB@@LB@@LB@Energy -15.55 kcal/mol@LB@Hybridization Energy -38.6272 kcal/mol@LB@Unfolding Energy - mRNA 10.4973 kcal/mol@LB@Unfolding Energy - sRNA 14.833 kcal/mol@LB@@LB@Position - mRNA 1 -- 63@LB@Position - sRNA 31 -- 85@LB@Position Seed - mRNA 36 -- 42@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 133 |
0.006226 |
0.1741 |
bsu14430 |
ykpA |
-10.78 |
0.228396 |
48 -- 105 |
75 -- 129 |
ABC transporter ATP-binding protein |
|
_____________47____________106\n_____________|_____________|\n5'-GUA...AAGUU_____________AAUAU...UUG-3'\n______________AUUUGAAGAUGUU\n______________|||||||:|:|:|\n______________UAAACUUUUGCGA\n3'-AUU...AACUU_____________AUUAA...CAA-5'\n_____________|_____________|\n_____________130___________74\n |
254 -- 260 |
15 -- 21 |
-11.9489 kcal/mol |
0.844332 kcal/mol |
3.96844 kcal/mol |
47 106@LB@ | |@LB@5'-GUA...AAGUU AAUAU...UUG-3'@LB@ AUUUGAAGAUGUU@LB@ |||||||:|:|:|@LB@ UAAACUUUUGCGA@LB@3'-AUU...AACUU AUUAA...CAA-5'@LB@ | |@LB@ 130 74@LB@@LB@@LB@Energy -10.78 kcal/mol@LB@Hybridization Energy -11.9489 kcal/mol@LB@Unfolding Energy - mRNA 0.844332 kcal/mol@LB@Unfolding Energy - sRNA 3.96844 kcal/mol@LB@@LB@Position - mRNA 48 -- 105@LB@Position - sRNA 75 -- 129@LB@Position Seed - mRNA 254 -- 260@LB@Position Seed - sRNA 15 -- 21@LB@ |
| 134 |
0.00626 |
0.1741 |
bsu03360 |
yciC |
-8.59 |
0.518951 |
99 -- 108 |
130 -- 139 |
metal chaperone YciC |
|
_____________98_______109\n_____________|________|\n5'-AGG...GGGCG________AAAUG...UAG-3'\n______________GCAGCGAG\n______________|||||:||\n______________CGUCGUUC\n3'-AUU...ACAGG________AAGGU...CAA-5'\n_____________|________|\n_____________140______129\n |
102 -- 108 |
130 -- 136 |
-12.856 kcal/mol |
2.76877 kcal/mol |
2.9981 kcal/mol |
98 109@LB@ | |@LB@5'-AGG...GGGCG AAAUG...UAG-3'@LB@ GCAGCGAG@LB@ |||||:||@LB@ CGUCGUUC@LB@3'-AUU...ACAGG AAGGU...CAA-5'@LB@ | |@LB@ 140 129@LB@@LB@@LB@Energy -8.59 kcal/mol@LB@Hybridization Energy -12.856 kcal/mol@LB@Unfolding Energy - mRNA 2.76877 kcal/mol@LB@Unfolding Energy - sRNA 2.9981 kcal/mol@LB@@LB@Position - mRNA 99 -- 108@LB@Position - sRNA 130 -- 139@LB@Position Seed - mRNA 102 -- 108@LB@Position Seed - sRNA 130 -- 136@LB@ |
| 135 |
0.00631 |
0.1741 |
bsu31510 |
yufK |
-14.38 |
0.044651 |
233 -- 280 |
42 -- 85 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________232________________________________________________281\n_____________|__________________________________________________|\n5'-UGC...UUUCC___GA_________CU___UUUU___U____CCUU_________AAC___ACUUU...UGC-3'\n______________GCU___UUGCGAUC___GC____UAG_UCGU____AUCCAUCUC___UUC\n______________|||___||||:|||___||____:|:_||||____|:|||||||___|||\n______________CGA___AACGUUAG___CG____GUU_AGCA____UGGGUAGAG___AAG\n3'-AUU...CUUAC___AGC________AAC__UUU______________________AAU___GGGUA...CAA-5'\n_____________|__________________________________________________|\n_____________86_________________________________________________41\n |
268 -- 274 |
48 -- 54 |
-29.5205 kcal/mol |
6.02241 kcal/mol |
9.1918 kcal/mol |
232 281@LB@ | |@LB@5'-UGC...UUUCC GA CU UUUU U CCUU AAC ACUUU...UGC-3'@LB@ GCU UUGCGAUC GC UAG UCGU AUCCAUCUC UUC@LB@ ||| ||||:||| || :|: |||| |:||||||| |||@LB@ CGA AACGUUAG CG GUU AGCA UGGGUAGAG AAG@LB@3'-AUU...CUUAC AGC AAC UUU AAU GGGUA...CAA-5'@LB@ | |@LB@ 86 41@LB@@LB@@LB@Energy -14.38 kcal/mol@LB@Hybridization Energy -29.5205 kcal/mol@LB@Unfolding Energy - mRNA 6.02241 kcal/mol@LB@Unfolding Energy - sRNA 9.1918 kcal/mol@LB@@LB@Position - mRNA 233 -- 280@LB@Position - sRNA 42 -- 85@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 48 -- 54@LB@ |
| 136 |
0.006329 |
0.1741 |
bsu10210 |
yhfF |
-9.29 |
0.409483 |
117 -- 143 |
37 -- 59 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________116______________144\n_____________|________________|\n5'-AUA...GACGC_______GCA______CGCAC...CCA-3'\n______________UUCCCAU___UUUGAA\n______________|:|||||___||||||\n______________AGGGGUA___AAACUU\n3'-AUU...GAAUA_______AAG______UAAAC...CAA-5'\n_____________|________________|\n_____________60_______________36\n |
101 -- 107 |
37 -- 43 |
-14.7282 kcal/mol |
3.20386 kcal/mol |
5.02573 kcal/mol |
116 144@LB@ | |@LB@5'-AUA...GACGC GCA CGCAC...CCA-3'@LB@ UUCCCAU UUUGAA@LB@ |:||||| ||||||@LB@ AGGGGUA AAACUU@LB@3'-AUU...GAAUA AAG UAAAC...CAA-5'@LB@ | |@LB@ 60 36@LB@@LB@@LB@Energy -9.29 kcal/mol@LB@Hybridization Energy -14.7282 kcal/mol@LB@Unfolding Energy - mRNA 3.20386 kcal/mol@LB@Unfolding Energy - sRNA 5.02573 kcal/mol@LB@@LB@Position - mRNA 117 -- 143@LB@Position - sRNA 37 -- 59@LB@Position Seed - mRNA 101 -- 107@LB@Position Seed - sRNA 37 -- 43@LB@ |
| 137 |
0.006339 |
0.1741 |
bsu39320 |
yxiB |
-11.20 |
0.191268 |
188 -- 222 |
111 -- 147 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________187_________________________223\n_____________|___________________________|\n5'-UAA...GUGUU______UGCAUA___A___________AUUCG...AAA-3'\n______________GCGGCA______GUG_AAUGUUGUUAC\n______________||:|||______:|:_||||||||:||\n______________CGUCGU______UAU_UUACAACAGUG\n3'-AUU...ACAGG______UCAAGG___A___________AAAGA...CAA-5'\n_____________|___________________________|\n_____________148_________________________110\n |
216 -- 222 |
111 -- 117 |
-23.3412 kcal/mol |
7.76229 kcal/mol |
5.28835 kcal/mol |
187 223@LB@ | |@LB@5'-UAA...GUGUU UGCAUA A AUUCG...AAA-3'@LB@ GCGGCA GUG AAUGUUGUUAC@LB@ ||:||| :|: ||||||||:||@LB@ CGUCGU UAU UUACAACAGUG@LB@3'-AUU...ACAGG UCAAGG A AAAGA...CAA-5'@LB@ | |@LB@ 148 110@LB@@LB@@LB@Energy -11.20 kcal/mol@LB@Hybridization Energy -23.3412 kcal/mol@LB@Unfolding Energy - mRNA 7.76229 kcal/mol@LB@Unfolding Energy - sRNA 5.28835 kcal/mol@LB@@LB@Position - mRNA 188 -- 222@LB@Position - sRNA 111 -- 147@LB@Position Seed - mRNA 216 -- 222@LB@Position Seed - sRNA 111 -- 117@LB@ |
| 138 |
0.00639 |
0.1741 |
bsu24900 |
rpmG |
-18.16 |
0.007101 |
92 -- 175 |
92 -- 166 |
50S ribosomal protein L33 |
|
_____________91_________________________________________________________176\n_____________|__________________________________________________________|\n5'-CGC...GCGUA______AUAAU______AUU______AAAACAG________UUCCG____U_______GUAUU...AAA-3'\n______________UCUUCA_____UCAGCA___CAUUGA_______CC_UAGCA_____UCCA_UAUAGUG\n______________||||||_____|||:||___||||||_______||_:||||_____||||_||||:||\n______________AGAAGU_____AGUUGU___GUAACU_______GG_GUCGU_____AGGU_AUAUUAC\n3'-AUU...AAAUG_________________CCU______GUAACA___C_____UCA______________AACAG...CAA-5'\n_____________|__________________________________________________________|\n_____________167________________________________________________________91\n |
142 -- 148 |
118 -- 124 |
-35.8515 kcal/mol |
8.17901 kcal/mol |
10.6225 kcal/mol |
91 176@LB@ | |@LB@5'-CGC...GCGUA AUAAU AUU AAAACAG UUCCG U GUAUU...AAA-3'@LB@ UCUUCA UCAGCA CAUUGA CC UAGCA UCCA UAUAGUG@LB@ |||||| |||:|| |||||| || :|||| |||| ||||:||@LB@ AGAAGU AGUUGU GUAACU GG GUCGU AGGU AUAUUAC@LB@3'-AUU...AAAUG CCU GUAACA C UCA AACAG...CAA-5'@LB@ | |@LB@ 167 91@LB@@LB@@LB@Energy -18.16 kcal/mol@LB@Hybridization Energy -35.8515 kcal/mol@LB@Unfolding Energy - mRNA 8.17901 kcal/mol@LB@Unfolding Energy - sRNA 10.6225 kcal/mol@LB@@LB@Position - mRNA 92 -- 175@LB@Position - sRNA 92 -- 166@LB@Position Seed - mRNA 142 -- 148@LB@Position Seed - sRNA 118 -- 124@LB@ |
| 139 |
0.006395 |
0.1741 |
bsu30170 |
ytcP |
-13.74 |
0.060669 |
6 -- 28 |
1 -- 23 |
ABC transporter permease |
|
_____________5_______________________29\n_____________|_______________________|\n______5'-UCUAA__UU_____CUU___________AUCGG...UUG-3'\n______________GA__AUGCU___UUUCUUUUGUU\n______________||__|:|||___|||||||||||\n______________CU__UGCGA___AAAGAAAACAA\n3'-AUU...UUAAA__UU_____AUU___________-5'\n_____________|______________________|\n_____________24_____________________1\n |
22 -- 28 |
1 -- 7 |
-18.7227 kcal/mol |
2.34195 kcal/mol |
4.12892 kcal/mol |
5 29@LB@ | |@LB@ 5'-UCUAA UU CUU AUCGG...UUG-3'@LB@ GA AUGCU UUUCUUUUGUU@LB@ || |:||| |||||||||||@LB@ CU UGCGA AAAGAAAACAA@LB@3'-AUU...UUAAA UU AUU -5'@LB@ | |@LB@ 24 1@LB@@LB@@LB@Energy -13.74 kcal/mol@LB@Hybridization Energy -18.7227 kcal/mol@LB@Unfolding Energy - mRNA 2.34195 kcal/mol@LB@Unfolding Energy - sRNA 4.12892 kcal/mol@LB@@LB@Position - mRNA 6 -- 28@LB@Position - sRNA 1 -- 23@LB@Position Seed - mRNA 22 -- 28@LB@Position Seed - sRNA 1 -- 7@LB@ |
| 140 |
0.006451 |
0.1741 |
bsu29090 |
polA |
-11.51 |
0.167440 |
58 -- 84 |
30 -- 56 |
DNA polymerase I |
|
_____________57________________85\n_____________|_________________|\n5'-AUG...CGGGA________G________GUCCU...AUA-3'\n______________ACCUAUUU__UGUUCCC\n______________|||:||:|__|:|||||\n______________UGGGUAGA__AUAAGGG\n3'-AUU...UAGCA________GA_______GUAAA...CAA-5'\n_____________|_________________|\n_____________57________________29\n |
67 -- 73 |
40 -- 46 |
-17.5976 kcal/mol |
2.41953 kcal/mol |
7.22212 kcal/mol |
57 85@LB@ | |@LB@5'-AUG...CGGGA G GUCCU...AUA-3'@LB@ ACCUAUUU UGUUCCC@LB@ |||:||:| |:|||||@LB@ UGGGUAGA AUAAGGG@LB@3'-AUU...UAGCA GA GUAAA...CAA-5'@LB@ | |@LB@ 57 29@LB@@LB@@LB@Energy -11.51 kcal/mol@LB@Hybridization Energy -17.5976 kcal/mol@LB@Unfolding Energy - mRNA 2.41953 kcal/mol@LB@Unfolding Energy - sRNA 7.22212 kcal/mol@LB@@LB@Position - mRNA 58 -- 84@LB@Position - sRNA 30 -- 56@LB@Position Seed - mRNA 67 -- 73@LB@Position Seed - sRNA 40 -- 46@LB@ |
