Input and runtime details for job 3202356 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 3202356 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:02 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:24:35 CET 2018
	CopraRNA Finished & Post-Processing		@ Sat Feb 17 02:03:47 CET 2018
	Post-Processing Finished		@ Sat Feb 17 02:03:55 CET 2018
	Job Completed		@ Sat Feb 17 02:04:28 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=3202356&toolName=CopraRNA ( 30 days expiry )

Description of the job

GcvB

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_003197 NC_003277 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
116	0.005629	0.1728	stm2662	rluD	-17.46	0.046576	204 -- 257	36 -- 86	23S rRNA pseudouridine(1911/1915/1917) synthase		_____________203____________________258\n_____________\|______________________\|\n5'-UGA...UAUAU__________G___________CGCAA...GUU-3'\n______________GGCACAACGA_UACAAC_UCAC\n______________::\|\|\|\|\|\|:\|_:\|\|\|\|\|_\|\|\|\|\n______________UUGUGUUGUU_GUGUUG_AGUG\n3'-UUU...AACGU_________________U____UUGGC...UCA-5'\n_____________\|______________________\|\n_____________87_____________________35\n	206 -- 212	78 -- 84	-22.2469 kcal/mol	4.97176 kcal/mol	5.3513 kcal/mol	203 258@LB@ \| \|@LB@5'-UGA...UAUAU G CGCAA...GUU-3'@LB@ GGCACAACGA UACAAC UCAC@LB@ ::\|\|\|\|\|\|:\| :\|\|\|\|\| \|\|\|\|@LB@ UUGUGUUGUU GUGUUG AGUG@LB@3'-UUU...AACGU U UUGGC...UCA-5'@LB@ \| \|@LB@ 87 35@LB@@LB@@LB@Energy -17.46 kcal/mol@LB@Hybridization Energy -22.2469 kcal/mol@LB@Unfolding Energy - mRNA 4.97176 kcal/mol@LB@Unfolding Energy - sRNA 5.3513 kcal/mol@LB@@LB@Position - mRNA 204 -- 257@LB@Position - sRNA 36 -- 86@LB@Position Seed - mRNA 206 -- 212@LB@Position Seed - sRNA 78 -- 84@LB@
178	0.009774	0.1951	stm2667	pheA	-14.20	0.157815	177 -- 228	36 -- 93	chorismate mutase		_____________176__________________229\n_____________\|____________________\|\n5'-GAC...ACAAA___G______C_________GGAAA...UUG-3'\n______________AAA_GCAACA_UAUGACAUC\n______________\|\|\|_:\|\|\|\|\|_:\|::\|\|\|\|\|\n______________UUU_UGUUGU_GUGUUGUAG\n3'-UUU...UAACG___G______U_________UGUUG...UCA-5'\n_____________\|____________________\|\n_____________94___________________35\n	202 -- 208	69 -- 75	-17.8987 kcal/mol	1.45353 kcal/mol	4.11387 kcal/mol	176 229@LB@ \| \|@LB@5'-GAC...ACAAA G C GGAAA...UUG-3'@LB@ AAA GCAACA UAUGACAUC@LB@ \|\|\| :\|\|\|\|\| :\|::\|\|\|\|\|@LB@ UUU UGUUGU GUGUUGUAG@LB@3'-UUU...UAACG G U UGUUG...UCA-5'@LB@ \| \|@LB@ 94 35@LB@@LB@@LB@Energy -14.20 kcal/mol@LB@Hybridization Energy -17.8987 kcal/mol@LB@Unfolding Energy - mRNA 1.45353 kcal/mol@LB@Unfolding Energy - sRNA 4.11387 kcal/mol@LB@@LB@Position - mRNA 177 -- 228@LB@Position - sRNA 36 -- 93@LB@Position Seed - mRNA 202 -- 208@LB@Position Seed - sRNA 69 -- 75@LB@
103	0.005167	0.1728	stm2670	aroF	-17.31	0.049328	173 -- 242	25 -- 90	3-deoxy-7-phosphoheptulonate synthase		_____________172______________________________________________________________________243\n_____________\|________________________________________________________________________\|\n5'-CUU...CUAUC_______AGAA__G____G_____C___AU____A_____A_G____U___UAACG_______CA_______ACAGG...AGG-3'\n______________GCAAACA____AA_ACAG_AUCGC_AUC____GC_AAAAG_C_CG_C_GAA_____UACGUAU__CCGAUGA\n______________\|\|\|\|\|\|\|____\|\|_\|\|\|:_\|\|\|:\|_\|:\|____\|\|_\|\|\|\|\|_\|_\|:_\|_\|\|\|_____:\|\|\|:\|\|__\|\|\|\|\|:\|\n______________CGUUUGU____UU_UGUU_UAGUG_UGG____CG_UUUUC_G_GU_G_CUU_____GUGCGUA__GGCUAUU\n3'-UUU...GUUAA_______GUUG__G____G_____U___CAUU__G_______G__A_U_______________A________UUGAA...UCA-5'\n_____________\|________________________________________________________________________\|\n_____________91_______________________________________________________________________24\n	226 -- 232	34 -- 40	-46.7665 kcal/mol	10.372 kcal/mol	24.1795 kcal/mol	172 243@LB@ \| \|@LB@5'-CUU...CUAUC AGAA G G C AU A A G U UAACG CA ACAGG...AGG-3'@LB@ GCAAACA AA ACAG AUCGC AUC GC AAAAG C CG C GAA UACGUAU CCGAUGA@LB@ \|\|\|\|\|\|\| \|\| \|\|\|: \|\|\|:\| \|:\| \|\| \|\|\|\|\| \| \|: \| \|\|\| :\|\|\|:\|\| \|\|\|\|\|:\|@LB@ CGUUUGU UU UGUU UAGUG UGG CG UUUUC G GU G CUU GUGCGUA GGCUAUU@LB@3'-UUU...GUUAA GUUG G G U CAUU G G A U A UUGAA...UCA-5'@LB@ \| \|@LB@ 91 24@LB@@LB@@LB@Energy -17.31 kcal/mol@LB@Hybridization Energy -46.7665 kcal/mol@LB@Unfolding Energy - mRNA 10.372 kcal/mol@LB@Unfolding Energy - sRNA 24.1795 kcal/mol@LB@@LB@Position - mRNA 173 -- 242@LB@Position - sRNA 25 -- 90@LB@Position Seed - mRNA 226 -- 232@LB@Position Seed - sRNA 34 -- 40@LB@
167	0.009062	0.1932	stm2673	rplS	-14.99	0.118272	119 -- 180	38 -- 88	50S ribosomal protein L19		_____________118_________________181\n_____________\|___________________\|\n5'-GAU...GUUCA_____G____C________GCAGC...UUG-3'\n______________AAACA_AACA_GCACAACA\n______________\|\|\|\|\|_\|\|\|\|_:\|\|\|\|\|\|\|\n______________UUUGU_UUGU_UGUGUUGU\n3'-UUU...UAACG_____G_____________AGUGU...UCA-5'\n_____________\|___________________\|\n_____________89__________________37\n	126 -- 132	71 -- 77	-18.964 kcal/mol	3.18358 kcal/mol	3.68874 kcal/mol	118 181@LB@ \| \|@LB@5'-GAU...GUUCA G C GCAGC...UUG-3'@LB@ AAACA AACA GCACAACA@LB@ \|\|\|\|\| \|\|\|\| :\|\|\|\|\|\|\|@LB@ UUUGU UUGU UGUGUUGU@LB@3'-UUU...UAACG G AGUGU...UCA-5'@LB@ \| \|@LB@ 89 37@LB@@LB@@LB@Energy -14.99 kcal/mol@LB@Hybridization Energy -18.964 kcal/mol@LB@Unfolding Energy - mRNA 3.18358 kcal/mol@LB@Unfolding Energy - sRNA 3.68874 kcal/mol@LB@@LB@Position - mRNA 119 -- 180@LB@Position - sRNA 38 -- 88@LB@Position Seed - mRNA 126 -- 132@LB@Position Seed - sRNA 71 -- 77@LB@
132	0.006673	0.1774	stm2677	ffh	-17.54	0.045291	232 -- 275	38 -- 86	4.5S-RNP protein		_____________231_________________________________________________________________________________________________276\n_____________\|___________________________________________________________________________________________________\|\n5'-UAG...CAAAA_______UA__U_____CG____UU__CC__________A_A____A___A__G___UACG___C______CU____CC__U____________AA___GGCUU...AAG-3'\n______________ACGGGCA__GU__GUGU__ACAG__GA__AGUGAGAAAG_G_AG_C__GU_UA_CGU____UGA__CGCAG__CCAA__GU__GCAUAACAACA__GAC\n______________\|\|:\|:\|\|__\|\|__:\|\|\|__\|\|\|\|__\|\|__\|\|\|\|\|:\|\|\|\|_\|_\|\|_\|__\|:_\|\|_\|\|\|____\|\|\|__\|\|\|\|\|__\|\|\|\|__\|:__:\|\|:\|\|\|\|\|\|\|__:\|\|\n______________UGUCUGU__CA__UACA__UGUC__CU__UCACUUUUUC_C_UC_G__CG_AU_GCA____ACU__GCGUC__GGUU__CG__UGUGUUGUUGU__UUG\n3'-UUU...AUACC_______CC__UU__________________________C_A__A_AG_____G___CCAG___UA_____U_____AA__UU___________G____UAGUG...UCA-5'\n_____________\|___________________________________________________________________________________________________\|\n_____________87__________________________________________________________________________________________________37\n	110 -- 116	76 -- 82	-60.3799 kcal/mol	23.7315 kcal/mol	24.6218 kcal/mol	231 276@LB@ \| \|@LB@5'-UAG...CAAAA UA U CG UU CC A A A A G UACG C CU CC U AA GGCUU...AAG-3'@LB@ ACGGGCA GU GUGU ACAG GA AGUGAGAAAG G AG C GU UA CGU UGA CGCAG CCAA GU GCAUAACAACA GAC@LB@ \|\|:\|:\|\| \|\| :\|\|\| \|\|\|\| \|\| \|\|\|\|\|:\|\|\|\| \| \|\| \| \|: \|\| \|\|\| \|\|\| \|\|\|\|\| \|\|\|\| \|: :\|\|:\|\|\|\|\|\|\| :\|\|@LB@ UGUCUGU CA UACA UGUC CU UCACUUUUUC C UC G CG AU GCA ACU GCGUC GGUU CG UGUGUUGUUGU UUG@LB@3'-UUU...AUACC CC UU C A A AG G CCAG UA U AA UU G UAGUG...UCA-5'@LB@ \| \|@LB@ 87 37@LB@@LB@@LB@Energy -17.54 kcal/mol@LB@Hybridization Energy -60.3799 kcal/mol@LB@Unfolding Energy - mRNA 23.7315 kcal/mol@LB@Unfolding Energy - sRNA 24.6218 kcal/mol@LB@@LB@Position - mRNA 232 -- 275@LB@Position - sRNA 38 -- 86@LB@Position Seed - mRNA 110 -- 116@LB@Position Seed - sRNA 76 -- 82@LB@
111	0.005436	0.1728	stm2683	yfjB	-15.36	0.103307	255 -- 299	43 -- 90	NAD(+) kinase		_____________254___________________________________________________________________300\n_____________\|_____________________________________________________________________\|\n5'-CUC...UUCGC__________GAU____UU______UUCAA__________ACUCACAUU_____AUCAAUCU_______AAGGA...AGG-3'\n______________UUAUUAUGGG___CAGA__CAGGGU________GGGAAGU___________GUC________UCGCUAC\n______________\|:\|:\|\|\|\|\|:___\|\|\|\|__\|\|\|\|\|\|________:\|\|\|\|\|\|___________\|\|\|________\|\|\|\|\|\|\|\n______________AGUGAUACCU___GUCU__GUCCCA________UCCUUCA___________CAG________AGCGAUG\n3'-UUU...UAAUU_________________________UUUACAUG_______CUUUUUCCCAU__________________GCACC...UCA-5'\n_____________\|_____________________________________________________________________\|\n_____________91____________________________________________________________________42\n	179 -- 185	115 -- 121	-40.6869 kcal/mol	14.2494 kcal/mol	12.858 kcal/mol	254 300@LB@ \| \|@LB@5'-CUC...UUCGC GAU UU UUCAA ACUCACAUU AUCAAUCU AAGGA...AGG-3'@LB@ UUAUUAUGGG CAGA CAGGGU GGGAAGU GUC UCGCUAC@LB@ \|:\|:\|\|\|\|\|: \|\|\|\| \|\|\|\|\|\| :\|\|\|\|\|\| \|\|\| \|\|\|\|\|\|\|@LB@ AGUGAUACCU GUCU GUCCCA UCCUUCA CAG AGCGAUG@LB@3'-UUU...UAAUU UUUACAUG CUUUUUCCCAU GCACC...UCA-5'@LB@ \| \|@LB@ 91 42@LB@@LB@@LB@Energy -15.36 kcal/mol@LB@Hybridization Energy -40.6869 kcal/mol@LB@Unfolding Energy - mRNA 14.2494 kcal/mol@LB@Unfolding Energy - sRNA 12.858 kcal/mol@LB@@LB@Position - mRNA 255 -- 299@LB@Position - sRNA 43 -- 90@LB@Position Seed - mRNA 179 -- 185@LB@Position Seed - sRNA 115 -- 121@LB@
70	0.003306	0.1596	stm2688	smpB	-12.25	0.308628	191 -- 230	36 -- 77	SsrA-binding protein		_____________190_________________231\n_____________\|___________________\|\n5'-AAU...GCAUA____UUCC__U________AUCGU...GUC-3'\n______________GCAA____CA_CAACAUAA\n______________\|\|\|\|____\|\|_\|\|\|\|\|:\|\|\n______________CGUU____GU_GUUGUGUU\n3'-UUU...GUUAA____UGU___U________GUAGU...UCA-5'\n_____________\|___________________\|\n_____________78__________________35\n	26 -- 32	73 -- 79	-12.551 kcal/mol	1.44298 kcal/mol	3.77104 kcal/mol	190 231@LB@ \| \|@LB@5'-AAU...GCAUA UUCC U AUCGU...GUC-3'@LB@ GCAA CA CAACAUAA@LB@ \|\|\|\| \|\| \|\|\|\|\|:\|\|@LB@ CGUU GU GUUGUGUU@LB@3'-UUU...GUUAA UGU U GUAGU...UCA-5'@LB@ \| \|@LB@ 78 35@LB@@LB@@LB@Energy -12.25 kcal/mol@LB@Hybridization Energy -12.551 kcal/mol@LB@Unfolding Energy - mRNA 1.44298 kcal/mol@LB@Unfolding Energy - sRNA 3.77104 kcal/mol@LB@@LB@Position - mRNA 191 -- 230@LB@Position - sRNA 36 -- 77@LB@Position Seed - mRNA 26 -- 32@LB@Position Seed - sRNA 73 -- 79@LB@
51	0.001912	0.1324	stm2692	N/A	-10.31	0.546971	162 -- 173	130 -- 141	putative HlyD family secretion protein		_____________161__________174\n_____________\|____________\|\n5'-GUU...CCGUC____________AUGAA...CGU-3'\n______________GGGAGUGGAAAA\n______________\|\|:\|\|\|\|:\|\|\|\|\n______________CCUUCACUUUUU\n3'-UUU...CAUGU____________CCCAU...UCA-5'\n_____________\|____________\|\n_____________142__________129\n	166 -- 172	131 -- 137	-15.3652 kcal/mol	2.02429 kcal/mol	2.83702 kcal/mol	161 174@LB@ \| \|@LB@5'-GUU...CCGUC AUGAA...CGU-3'@LB@ GGGAGUGGAAAA@LB@ \|\|:\|\|\|\|:\|\|\|\|@LB@ CCUUCACUUUUU@LB@3'-UUU...CAUGU CCCAU...UCA-5'@LB@ \| \|@LB@ 142 129@LB@@LB@@LB@Energy -10.31 kcal/mol@LB@Hybridization Energy -15.3652 kcal/mol@LB@Unfolding Energy - mRNA 2.02429 kcal/mol@LB@Unfolding Energy - sRNA 2.83702 kcal/mol@LB@@LB@Position - mRNA 162 -- 173@LB@Position - sRNA 130 -- 141@LB@Position Seed - mRNA 166 -- 172@LB@Position Seed - sRNA 131 -- 137@LB@
17	0.0003055	0.06194	stm2694	N/A	-22.95	0.005498	261 -- 295	52 -- 90	Fels-2 prophage protein		_____________260_____________________________________296\n_____________\|_______________________________________\|\n5'-ACG...UAUCA_____________A_G____________AGU________UAUAA-3'\n______________GC___UACGACAA_A_AACGUUACAACC____GCCAAAA\n______________\|\|___:\|\|:\|\|\|\|_\|_\|\|\|:\|:\|\|\|\|\|\|____\|\|\|\|\|\|\|\n______________CG___GUGUUGUU_U_UUGUAGUGUUGG____CGGUUUU\n3'-UUU...GUUAA__UUU________G_G____________CAUU_______CGGGU...UCA-5'\n_____________\|_______________________________________\|\n_____________91______________________________________51\n	289 -- 295	52 -- 58	-35.4487 kcal/mol	2.51857 kcal/mol	14.0353 kcal/mol	260 296@LB@ \| \|@LB@5'-ACG...UAUCA A G AGU UAUAA-3'@LB@ GC UACGACAA A AACGUUACAACC GCCAAAA@LB@ \|\| :\|\|:\|\|\|\| \| \|\|\|:\|:\|\|\|\|\|\| \|\|\|\|\|\|\|@LB@ CG GUGUUGUU U UUGUAGUGUUGG CGGUUUU@LB@3'-UUU...GUUAA UUU G G CAUU CGGGU...UCA-5'@LB@ \| \|@LB@ 91 51@LB@@LB@@LB@Energy -22.95 kcal/mol@LB@Hybridization Energy -35.4487 kcal/mol@LB@Unfolding Energy - mRNA 2.51857 kcal/mol@LB@Unfolding Energy - sRNA 14.0353 kcal/mol@LB@@LB@Position - mRNA 261 -- 295@LB@Position - sRNA 52 -- 90@LB@Position Seed - mRNA 289 -- 295@LB@Position Seed - sRNA 52 -- 58@LB@
34	0.001111	0.1164	stm2746	N/A	-14.94	0.120804	53 -- 101	36 -- 90	putative excinuclease ATPase subunit	stm4494 stm3753	_____________52_____________________102\n_____________\|______________________\|\n5'-GGA...UUGAA____UA_________U______UCAAG...AUG-3'\n______________GCAA__ACAACAACG__ACAUU\n______________\|\|\|\|__\|\|\|\|\|\|\|\|:__\|\|\|\|:\n______________CGUU__UGUUGUUGU__UGUAG\n3'-UUU...GUUAA____UG_________GU_____UGUUG...UCA-5'\n_____________\|______________________\|\n_____________91_____________________35\n	61 -- 67	76 -- 82	-20.4267 kcal/mol	3.3222 kcal/mol	4.33922 kcal/mol	52 102@LB@ \| \|@LB@5'-GGA...UUGAA UA U UCAAG...AUG-3'@LB@ GCAA ACAACAACG ACAUU@LB@ \|\|\|\| \|\|\|\|\|\|\|\|: \|\|\|\|:@LB@ CGUU UGUUGUUGU UGUAG@LB@3'-UUU...GUUAA UG GU UGUUG...UCA-5'@LB@ \| \|@LB@ 91 35@LB@@LB@@LB@Energy -14.94 kcal/mol@LB@Hybridization Energy -20.4267 kcal/mol@LB@Unfolding Energy - mRNA 3.3222 kcal/mol@LB@Unfolding Energy - sRNA 4.33922 kcal/mol@LB@@LB@Position - mRNA 53 -- 101@LB@Position - sRNA 36 -- 90@LB@Position Seed - mRNA 61 -- 67@LB@Position Seed - sRNA 76 -- 82@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

Use the following button if you want to resubmit the job with altered input or parameterization:

usability assessment

Please support us by filling this short quality survey for evaluating this

service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

Main Menu

Freiburg RNA Tools

Interaction Prediction

Seq-Str Alignment

CRISPR

Sequence Design

Splicing

SNP & Mutation

Classification

Gene set extension

MoDPepInt Server

CPSP-Tools Server

Galaxy-FR

CMV server

Teaching