Input and runtime details for job 1786318 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1786318 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:20:27 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:20:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:01:46 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:01:53 CET 2018
	Job Completed		@ Fri Feb 16 22:02:22 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1786318&toolName=CopraRNA ( 30 days expiry )

Description of the job

ArcZ

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
176	0.01044	0.208	b0933	ssuB	-9.69	0.158756	203 -- 226	69 -- 90	aliphatic sulfonate ABC transporter ATPase		_____________202______________________227\n_____________\|________________________\|\n5'-AUU...GGUAU_________UC___UGA_______CACGC...AUA-3'\n______________GAAUACUGC__GUC___ACCAGGG\n______________\|\|\|\|\|\|\|\|\|__\|:\|___\|\|\|\|\|\|\|\n______________CUUAUGACG__CGG___UGGUCCC\n3'-UUU...ACGCG______________UUG_______UUUAG...GUG-5'\n_____________\|________________________\|\n_____________91_______________________68\n	220 -- 226	69 -- 75	-28.101 kcal/mol	8.24441 kcal/mol	10.1628 kcal/mol	202 227@LB@ \| \|@LB@5'-AUU...GGUAU UC UGA CACGC...AUA-3'@LB@ GAAUACUGC GUC ACCAGGG@LB@ \|\|\|\|\|\|\|\|\| \|:\| \|\|\|\|\|\|\|@LB@ CUUAUGACG CGG UGGUCCC@LB@3'-UUU...ACGCG UUG UUUAG...GUG-5'@LB@ \| \|@LB@ 91 68@LB@@LB@@LB@Energy -9.69 kcal/mol@LB@Hybridization Energy -28.101 kcal/mol@LB@Unfolding Energy - mRNA 8.24441 kcal/mol@LB@Unfolding Energy - sRNA 10.1628 kcal/mol@LB@@LB@Position - mRNA 203 -- 226@LB@Position - sRNA 69 -- 90@LB@Position Seed - mRNA 220 -- 226@LB@Position Seed - sRNA 69 -- 75@LB@
161	0.009429	0.2062	b1372	stfR	-7.17	0.431291	283 -- 297	69 -- 83	Rac prophage; putative tail fiber protein	b1547	_____________282_____________298\n_____________\|_______________\|\n5'-CAC...AAAAC__AA________C__UGG-3'\n______________GU__CAGCACCA_GG\n______________\|:__\|\|:\|\|\|\|\|_\|\|\n______________CG__GUUGUGGU_CC\n3'-UUU...UAUGA__CG________C__UUUAG...GUG-5'\n_____________\|_______________\|\n_____________84______________68\n	288 -- 294	72 -- 78	-17.3245 kcal/mol	2.81769 kcal/mol	7.33837 kcal/mol	282 298@LB@ \| \|@LB@5'-CAC...AAAAC AA C UGG-3'@LB@ GU CAGCACCA GG@LB@ \|: \|\|:\|\|\|\|\| \|\|@LB@ CG GUUGUGGU CC@LB@3'-UUU...UAUGA CG C UUUAG...GUG-5'@LB@ \| \|@LB@ 84 68@LB@@LB@@LB@Energy -7.17 kcal/mol@LB@Hybridization Energy -17.3245 kcal/mol@LB@Unfolding Energy - mRNA 2.81769 kcal/mol@LB@Unfolding Energy - sRNA 7.33837 kcal/mol@LB@@LB@Position - mRNA 283 -- 297@LB@Position - sRNA 69 -- 83@LB@Position Seed - mRNA 288 -- 294@LB@Position Seed - sRNA 72 -- 78@LB@
168	0.009959	0.2071	b0727	sucB	-11.51	0.071542	30 -- 47	72 -- 88	dihydrolipoyltranssuccinase		_____________29_________________48\n_____________\|__________________\|\n5'-CAG...GCAUG_______A__________UUUCC...GUG-3'\n______________GUACUGC_GCCAGCAUCA\n______________:\|\|\|\|\|\|_\|\|\|\|:\|\|:\|\|\n______________UAUGACG_CGGUUGUGGU\n3'-UUU...GCGCU__________________CCCUU...GUG-5'\n_____________\|__________________\|\n_____________89_________________71\n	38 -- 44	75 -- 81	-25.7274 kcal/mol	6.97777 kcal/mol	7.24148 kcal/mol	29 48@LB@ \| \|@LB@5'-CAG...GCAUG A UUUCC...GUG-3'@LB@ GUACUGC GCCAGCAUCA@LB@ :\|\|\|\|\|\| \|\|\|\|:\|\|:\|\|@LB@ UAUGACG CGGUUGUGGU@LB@3'-UUU...GCGCU CCCUU...GUG-5'@LB@ \| \|@LB@ 89 71@LB@@LB@@LB@Energy -11.51 kcal/mol@LB@Hybridization Energy -25.7274 kcal/mol@LB@Unfolding Energy - mRNA 6.97777 kcal/mol@LB@Unfolding Energy - sRNA 7.24148 kcal/mol@LB@@LB@Position - mRNA 30 -- 47@LB@Position - sRNA 72 -- 88@LB@Position Seed - mRNA 38 -- 44@LB@Position Seed - sRNA 75 -- 81@LB@
181	0.0108	0.2089	b1683	sufB	-11.33	0.077347	153 -- 175	52 -- 74	component of SufBCD Fe-S cluster assembly scaffold		_____________152_______________________176\n_____________\|_________________________\|\n5'-GUU...AAAGC____AAU___UG__________G__AAAGC...AGC-3'\n______________CCAG___GAA____UGGCUGUG_CG\n______________\|\|\|\|___\|\|\|____\|\|\|\|\|\|:\|_\|\|\n______________GGUC___CUU____ACCGACGC_GC\n3'-UUU...GUUGU____C_____UAGA________G__AUUUA...GUG-5'\n_____________\|_________________________\|\n_____________75________________________51\n	166 -- 172	55 -- 61	-23.8546 kcal/mol	3.76094 kcal/mol	8.7601 kcal/mol	152 176@LB@ \| \|@LB@5'-GUU...AAAGC AAU UG G AAAGC...AGC-3'@LB@ CCAG GAA UGGCUGUG CG@LB@ \|\|\|\| \|\|\| \|\|\|\|\|\|:\| \|\|@LB@ GGUC CUU ACCGACGC GC@LB@3'-UUU...GUUGU C UAGA G AUUUA...GUG-5'@LB@ \| \|@LB@ 75 51@LB@@LB@@LB@Energy -11.33 kcal/mol@LB@Hybridization Energy -23.8546 kcal/mol@LB@Unfolding Energy - mRNA 3.76094 kcal/mol@LB@Unfolding Energy - sRNA 8.7601 kcal/mol@LB@@LB@Position - mRNA 153 -- 175@LB@Position - sRNA 52 -- 74@LB@Position Seed - mRNA 166 -- 172@LB@Position Seed - sRNA 55 -- 61@LB@
27	0.001577	0.1727	b1679	sufE	-9.94	0.142549	247 -- 262	68 -- 86	sulfur acceptor protein		_____________246_________________263\n_____________\|___________________\|\n5'-AUG...UUUAC___________U_______AGAGA...GUC-3'\n______________GCUGCGCCAAC____GGGA\n______________:\|\|\|\|\|\|\|\|\|\|____\|\|\|\|\n______________UGACGCGGUUG____CCCU\n3'-UUU...GCUUA___________UGGU____UUAGA...GUG-5'\n_____________\|___________________\|\n_____________87__________________67\n	251 -- 257	76 -- 82	-25.4399 kcal/mol	8.12138 kcal/mol	7.37523 kcal/mol	246 263@LB@ \| \|@LB@5'-AUG...UUUAC U AGAGA...GUC-3'@LB@ GCUGCGCCAAC GGGA@LB@ :\|\|\|\|\|\|\|\|\|\| \|\|\|\|@LB@ UGACGCGGUUG CCCU@LB@3'-UUU...GCUUA UGGU UUAGA...GUG-5'@LB@ \| \|@LB@ 87 67@LB@@LB@@LB@Energy -9.94 kcal/mol@LB@Hybridization Energy -25.4399 kcal/mol@LB@Unfolding Energy - mRNA 8.12138 kcal/mol@LB@Unfolding Energy - sRNA 7.37523 kcal/mol@LB@@LB@Position - mRNA 247 -- 262@LB@Position - sRNA 68 -- 86@LB@Position Seed - mRNA 251 -- 257@LB@Position Seed - sRNA 76 -- 82@LB@
124	0.006738	0.1913	b2533	suhB	-10.44	0.114821	158 -- 166	51 -- 59	inositol monophosphatase		_____________157_______167\n_____________\|_________\|\n5'-UUG...AUACU_________UUUCC...AGA-3'\n______________CUGCGCCGU\n______________\|\|\|\|\|\|\|\|\|\n______________GACGCGGCA\n3'-UUU...GAACC_________UUUAA...GUG-5'\n_____________\|_________\|\n_____________60________50\n	160 -- 166	51 -- 57	-17.2912 kcal/mol	2.02049 kcal/mol	4.83013 kcal/mol	157 167@LB@ \| \|@LB@5'-UUG...AUACU UUUCC...AGA-3'@LB@ CUGCGCCGU@LB@ \|\|\|\|\|\|\|\|\|@LB@ GACGCGGCA@LB@3'-UUU...GAACC UUUAA...GUG-5'@LB@ \| \|@LB@ 60 50@LB@@LB@@LB@Energy -10.44 kcal/mol@LB@Hybridization Energy -17.2912 kcal/mol@LB@Unfolding Energy - mRNA 2.02049 kcal/mol@LB@Unfolding Energy - sRNA 4.83013 kcal/mol@LB@@LB@Position - mRNA 158 -- 166@LB@Position - sRNA 51 -- 59@LB@Position Seed - mRNA 160 -- 166@LB@Position Seed - sRNA 51 -- 57@LB@
79	0.004357	0.1855	b0008	talB	-10.61	0.106795	240 -- 280	50 -- 88	transaldolase B		_____________239________________________________________281\n_____________\|__________________________________________\|\n5'-AGG...CCACC___G_________________C___GC___AAUGAA______UCAAC...CAA-3'\n______________GUA_UG_GCCGACACUGGGGA_AUC__GGC______GCUGUA\n______________:\|\|_\|\|_\|\|\|:\|\|\|\|::\|\|\|\|_\|\|\|__\|\|\|______\|\|:\|\|\|\n______________UAU_AC_CGGUUGUGGUCCCU_UAG__CCG______CGGCAU\n3'-UUU...GCGCU___G__G______________U___AA___ACG_________UUAAU...GUG-5'\n_____________\|__________________________________________\|\n_____________89_________________________________________49\n	253 -- 259	68 -- 74	-44.9099 kcal/mol	17.4497 kcal/mol	16.8544 kcal/mol	239 281@LB@ \| \|@LB@5'-AGG...CCACC G C GC AAUGAA UCAAC...CAA-3'@LB@ GUA UG GCCGACACUGGGGA AUC GGC GCUGUA@LB@ :\|\| \|\| \|\|\|:\|\|\|\|::\|\|\|\| \|\|\| \|\|\| \|\|:\|\|\|@LB@ UAU AC CGGUUGUGGUCCCU UAG CCG CGGCAU@LB@3'-UUU...GCGCU G G U AA ACG UUAAU...GUG-5'@LB@ \| \|@LB@ 89 49@LB@@LB@@LB@Energy -10.61 kcal/mol@LB@Hybridization Energy -44.9099 kcal/mol@LB@Unfolding Energy - mRNA 17.4497 kcal/mol@LB@Unfolding Energy - sRNA 16.8544 kcal/mol@LB@@LB@Position - mRNA 240 -- 280@LB@Position - sRNA 50 -- 88@LB@Position Seed - mRNA 253 -- 259@LB@Position Seed - sRNA 68 -- 74@LB@
191	0.01171	0.2159	b4221	tamB	-9.06	0.207346	81 -- 101	52 -- 76	translocation and assembly module for autotransporter export inner membrane subunit		_____________80_________________________102\n_____________\|__________________________\|\n5'-ACC...ACCGG__________________G_______GGAAU...AUC-3'\n______________UACCGGGG___UC__GGC_UGCGCUG\n______________:\|\|\|:\|\|\|___\|\|__\|\|\|_\|\|\|\|\|:\|\n______________GUGGUCCC___AG__CCG_ACGCGGC\n3'-UUU...CGGUU________UUU__AA___________AUUUA...GUG-5'\n_____________\|__________________________\|\n_____________77_________________________51\n	95 -- 101	52 -- 58	-29.4253 kcal/mol	11.0996 kcal/mol	9.26585 kcal/mol	80 102@LB@ \| \|@LB@5'-ACC...ACCGG G GGAAU...AUC-3'@LB@ UACCGGGG UC GGC UGCGCUG@LB@ :\|\|\|:\|\|\| \|\| \|\|\| \|\|\|\|\|:\|@LB@ GUGGUCCC AG CCG ACGCGGC@LB@3'-UUU...CGGUU UUU AA AUUUA...GUG-5'@LB@ \| \|@LB@ 77 51@LB@@LB@@LB@Energy -9.06 kcal/mol@LB@Hybridization Energy -29.4253 kcal/mol@LB@Unfolding Energy - mRNA 11.0996 kcal/mol@LB@Unfolding Energy - sRNA 9.26585 kcal/mol@LB@@LB@Position - mRNA 81 -- 101@LB@Position - sRNA 52 -- 76@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 52 -- 58@LB@
167	0.009922	0.2071	b0367	tauC	-5.98	0.628340	152 -- 162	85 -- 95	taurine ABC transporter permease		_____________151_________163\n_____________\|___________\|\n5'-AUA...CGCCA___________GUUUU...UUU-3'\n______________UGCGCGAAUAU\n______________\|\|\|\|\|\|\|\|\|\|:\n______________ACGCGCUUAUG\n3'-UUU...GCCCC___________ACGCG...GUG-5'\n_____________\|___________\|\n_____________96__________84\n	154 -- 160	87 -- 93	-16.0697 kcal/mol	4.17782 kcal/mol	5.90856 kcal/mol	151 163@LB@ \| \|@LB@5'-AUA...CGCCA GUUUU...UUU-3'@LB@ UGCGCGAAUAU@LB@ \|\|\|\|\|\|\|\|\|\|:@LB@ ACGCGCUUAUG@LB@3'-UUU...GCCCC ACGCG...GUG-5'@LB@ \| \|@LB@ 96 84@LB@@LB@@LB@Energy -5.98 kcal/mol@LB@Hybridization Energy -16.0697 kcal/mol@LB@Unfolding Energy - mRNA 4.17782 kcal/mol@LB@Unfolding Energy - sRNA 5.90856 kcal/mol@LB@@LB@Position - mRNA 152 -- 162@LB@Position - sRNA 85 -- 95@LB@Position Seed - mRNA 154 -- 160@LB@Position Seed - sRNA 87 -- 93@LB@
145	0.008374	0.2033	b0068	thiB	-7.61	0.367750	229 -- 244	73 -- 87	thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein		_____________228______________245\n_____________\|________________\|\n5'-UUU...GCUGU________A_______CGUUU...ACU-3'\n______________UGCUGUGC_CAGCGCC\n______________\|:\|\|\|:\|\|_\|\|:\|:\|\|\n______________AUGACGCG_GUUGUGG\n3'-UUU...CGCUU________________UCCCU...GUG-5'\n_____________\|________________\|\n_____________88_______________72\n	230 -- 236	80 -- 86	-23.483 kcal/mol	8.91653 kcal/mol	6.95602 kcal/mol	228 245@LB@ \| \|@LB@5'-UUU...GCUGU A CGUUU...ACU-3'@LB@ UGCUGUGC CAGCGCC@LB@ \|:\|\|\|:\|\| \|\|:\|:\|\|@LB@ AUGACGCG GUUGUGG@LB@3'-UUU...CGCUU UCCCU...GUG-5'@LB@ \| \|@LB@ 88 72@LB@@LB@@LB@Energy -7.61 kcal/mol@LB@Hybridization Energy -23.483 kcal/mol@LB@Unfolding Energy - mRNA 8.91653 kcal/mol@LB@Unfolding Energy - sRNA 6.95602 kcal/mol@LB@@LB@Position - mRNA 229 -- 244@LB@Position - sRNA 73 -- 87@LB@Position Seed - mRNA 230 -- 236@LB@Position Seed - sRNA 80 -- 86@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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