Input and runtime details for job 1786318 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1786318 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:20:27 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:20:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:01:46 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:01:53 CET 2018
	Job Completed		@ Fri Feb 16 22:02:22 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1786318&toolName=CopraRNA ( 30 days expiry )

Description of the job

ArcZ

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation	Additional homologs
121	0.006643	0.1913	b1813	nudL	-11.92	0.059353	114 -- 132	70 -- 86	putative CoA pyrophosphohydrolase weak 3-phosphohydroxypyruvate phosphatase		_____________113_________________133\n_____________\|___________________\|\n5'-CCU...GUGGA____U________G_____AAGAA...CUG-3'\n______________AUUG_CGCCGAUA_CCAGG\n______________\|:\|\|_\|\|\|\|:\|:\|_\|\|\|\|\|\n______________UGAC_GCGGUUGU_GGUCC\n3'-UUU...GCUUA___________________CUUUA...GUG-5'\n_____________\|___________________\|\n_____________87__________________69\n	120 -- 126	75 -- 81	-24.7024 kcal/mol	5.50013 kcal/mol	7.27772 kcal/mol	113 133@LB@ \| \|@LB@5'-CCU...GUGGA U G AAGAA...CUG-3'@LB@ AUUG CGCCGAUA CCAGG@LB@ \|:\|\| \|\|\|\|:\|:\| \|\|\|\|\|@LB@ UGAC GCGGUUGU GGUCC@LB@3'-UUU...GCUUA CUUUA...GUG-5'@LB@ \| \|@LB@ 87 69@LB@@LB@@LB@Energy -11.92 kcal/mol@LB@Hybridization Energy -24.7024 kcal/mol@LB@Unfolding Energy - mRNA 5.50013 kcal/mol@LB@Unfolding Energy - sRNA 7.27772 kcal/mol@LB@@LB@Position - mRNA 114 -- 132@LB@Position - sRNA 70 -- 86@LB@Position Seed - mRNA 120 -- 126@LB@Position Seed - sRNA 75 -- 81@LB@
122	0.006658	0.1913	b0600	ybdL	-10.23	0.125775	275 -- 297	68 -- 86	methionine aminotransferase PLP-dependent		_____________274_____________________298\n_____________\|_______________________\|\n5'-UCA...AGCGC___G____AG__________C__UUA-3'\n______________GCU_GCGC__CAACACCAGG_GA\n______________:\|\|_\|\|\|\|__\|\|\|\|\|\|\|\|\|\|_\|\|\n______________UGA_CGCG__GUUGUGGUCC_CU\n3'-UUU...GCUUA_______________________UUAGA...GUG-5'\n_____________\|_______________________\|\n_____________87______________________67\n	288 -- 294	70 -- 76	-27.7616 kcal/mol	10.1544 kcal/mol	7.37523 kcal/mol	274 298@LB@ \| \|@LB@5'-UCA...AGCGC G AG C UUA-3'@LB@ GCU GCGC CAACACCAGG GA@LB@ :\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\| \|\|@LB@ UGA CGCG GUUGUGGUCC CU@LB@3'-UUU...GCUUA UUAGA...GUG-5'@LB@ \| \|@LB@ 87 67@LB@@LB@@LB@Energy -10.23 kcal/mol@LB@Hybridization Energy -27.7616 kcal/mol@LB@Unfolding Energy - mRNA 10.1544 kcal/mol@LB@Unfolding Energy - sRNA 7.37523 kcal/mol@LB@@LB@Position - mRNA 275 -- 297@LB@Position - sRNA 68 -- 86@LB@Position Seed - mRNA 288 -- 294@LB@Position Seed - sRNA 70 -- 76@LB@
123	0.00672	0.1913	b0882	clpA	-10.14	0.130927	50 -- 58	66 -- 74	ATPase and specificity subunit of ClpA-ClpP ATP-dependent serine protease chaperone activity		_____________49________59\n_____________\|_________\|\n5'-UUU...CACUA_________GGCCA...CGC-3'\n______________CCAGGGGAA\n______________\|\|\|\|\|\|:\|\|\n______________GGUCCCUUU\n3'-UUU...GUUGU_________AGAAC...GUG-5'\n_____________\|_________\|\n_____________75________65\n	52 -- 58	66 -- 72	-13.8898 kcal/mol	0.70189 kcal/mol	3.04768 kcal/mol	49 59@LB@ \| \|@LB@5'-UUU...CACUA GGCCA...CGC-3'@LB@ CCAGGGGAA@LB@ \|\|\|\|\|\|:\|\|@LB@ GGUCCCUUU@LB@3'-UUU...GUUGU AGAAC...GUG-5'@LB@ \| \|@LB@ 75 65@LB@@LB@@LB@Energy -10.14 kcal/mol@LB@Hybridization Energy -13.8898 kcal/mol@LB@Unfolding Energy - mRNA 0.70189 kcal/mol@LB@Unfolding Energy - sRNA 3.04768 kcal/mol@LB@@LB@Position - mRNA 50 -- 58@LB@Position - sRNA 66 -- 74@LB@Position Seed - mRNA 52 -- 58@LB@Position Seed - sRNA 66 -- 72@LB@
124	0.006738	0.1913	b2533	suhB	-10.44	0.114821	158 -- 166	51 -- 59	inositol monophosphatase		_____________157_______167\n_____________\|_________\|\n5'-UUG...AUACU_________UUUCC...AGA-3'\n______________CUGCGCCGU\n______________\|\|\|\|\|\|\|\|\|\n______________GACGCGGCA\n3'-UUU...GAACC_________UUUAA...GUG-5'\n_____________\|_________\|\n_____________60________50\n	160 -- 166	51 -- 57	-17.2912 kcal/mol	2.02049 kcal/mol	4.83013 kcal/mol	157 167@LB@ \| \|@LB@5'-UUG...AUACU UUUCC...AGA-3'@LB@ CUGCGCCGU@LB@ \|\|\|\|\|\|\|\|\|@LB@ GACGCGGCA@LB@3'-UUU...GAACC UUUAA...GUG-5'@LB@ \| \|@LB@ 60 50@LB@@LB@@LB@Energy -10.44 kcal/mol@LB@Hybridization Energy -17.2912 kcal/mol@LB@Unfolding Energy - mRNA 2.02049 kcal/mol@LB@Unfolding Energy - sRNA 4.83013 kcal/mol@LB@@LB@Position - mRNA 158 -- 166@LB@Position - sRNA 51 -- 59@LB@Position Seed - mRNA 160 -- 166@LB@Position Seed - sRNA 51 -- 57@LB@
125	0.006795	0.1914	b3003	yghA	-11.52	0.071261	264 -- 274	70 -- 80	putative oxidoreductase	b2902	_____________263_________275\n_____________\|___________\|\n5'-AAC...AACAA___________CAUCC...UAC-3'\n______________CCGACGCCAGG\n______________\|\|:\|\|:\|\|\|\|\|\n______________GGUUGUGGUCC\n3'-UUU...GACGC___________CUUUA...GUG-5'\n_____________\|___________\|\n_____________81__________69\n	268 -- 274	70 -- 76	-20.3781 kcal/mol	2.1599 kcal/mol	6.70146 kcal/mol	263 275@LB@ \| \|@LB@5'-AAC...AACAA CAUCC...UAC-3'@LB@ CCGACGCCAGG@LB@ \|\|:\|\|:\|\|\|\|\|@LB@ GGUUGUGGUCC@LB@3'-UUU...GACGC CUUUA...GUG-5'@LB@ \| \|@LB@ 81 69@LB@@LB@@LB@Energy -11.52 kcal/mol@LB@Hybridization Energy -20.3781 kcal/mol@LB@Unfolding Energy - mRNA 2.1599 kcal/mol@LB@Unfolding Energy - sRNA 6.70146 kcal/mol@LB@@LB@Position - mRNA 264 -- 274@LB@Position - sRNA 70 -- 80@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 70 -- 76@LB@
126	0.006849	0.1914	b2930	yggF	-13.28	0.032133	176 -- 191	68 -- 83	fructose 1 6 bisphosphatase isozyme		_____________175______________192\n_____________\|________________\|\n5'-AUC...AUUUG________________GGCCG...GCC-3'\n______________GCGUUGAUAUCGGGGA\n______________\|\|\|:::\|:\|:\|:\|\|\|\|\n______________CGCGGUUGUGGUCCCU\n3'-UUU...UAUGA________________UUAGA...GUG-5'\n_____________\|________________\|\n_____________84_______________67\n	185 -- 191	68 -- 74	-24.7766 kcal/mol	4.1491 kcal/mol	7.34657 kcal/mol	175 192@LB@ \| \|@LB@5'-AUC...AUUUG GGCCG...GCC-3'@LB@ GCGUUGAUAUCGGGGA@LB@ \|\|\|:::\|:\|:\|:\|\|\|\|@LB@ CGCGGUUGUGGUCCCU@LB@3'-UUU...UAUGA UUAGA...GUG-5'@LB@ \| \|@LB@ 84 67@LB@@LB@@LB@Energy -13.28 kcal/mol@LB@Hybridization Energy -24.7766 kcal/mol@LB@Unfolding Energy - mRNA 4.1491 kcal/mol@LB@Unfolding Energy - sRNA 7.34657 kcal/mol@LB@@LB@Position - mRNA 176 -- 191@LB@Position - sRNA 68 -- 83@LB@Position Seed - mRNA 185 -- 191@LB@Position Seed - sRNA 68 -- 74@LB@
127	0.007097	0.1961	b4065	yjcE	-10.62	0.105957	81 -- 91	51 -- 62	putative cation/proton antiporter		_____________80___________92\n_____________\|____________\|\n5'-UAU...GGCAA____________UUUUA...UGC-3'\n______________UGGCUGC_CCGU\n______________\|\|\|\|\|\|\|_\|\|\|\|\n______________ACCGACG_GGCA\n3'-UUU...UUAGA_______C____UUUAA...GUG-5'\n_____________\|____________\|\n_____________63___________50\n	81 -- 87	56 -- 62	-18.8543 kcal/mol	3.34811 kcal/mol	4.8826 kcal/mol	80 92@LB@ \| \|@LB@5'-UAU...GGCAA UUUUA...UGC-3'@LB@ UGGCUGC CCGU@LB@ \|\|\|\|\|\|\| \|\|\|\|@LB@ ACCGACG GGCA@LB@3'-UUU...UUAGA C UUUAA...GUG-5'@LB@ \| \|@LB@ 63 50@LB@@LB@@LB@Energy -10.62 kcal/mol@LB@Hybridization Energy -18.8543 kcal/mol@LB@Unfolding Energy - mRNA 3.34811 kcal/mol@LB@Unfolding Energy - sRNA 4.8826 kcal/mol@LB@@LB@Position - mRNA 81 -- 91@LB@Position - sRNA 51 -- 62@LB@Position Seed - mRNA 81 -- 87@LB@Position Seed - sRNA 56 -- 62@LB@
128	0.007127	0.1961	b0696	kdpC	-9.30	0.187437	90 -- 106	44 -- 60	potassium translocating ATPase subunit C		_____________89________________107\n_____________\|_________________\|\n5'-GCG...GCCAU____________C____GGAUU...GGU-3'\n______________GUUGCGCCGUAA_UUAU\n______________\|:\|\|\|\|\|\|\|\|\|\|_\|\|\|\|\n______________CGACGCGGCAUU_AAUA\n3'-UUU...AGAAC____________U____AUACU...GUG-5'\n_____________\|_________________\|\n_____________61________________43\n	95 -- 101	49 -- 55	-22.6138 kcal/mol	6.57247 kcal/mol	6.74006 kcal/mol	89 107@LB@ \| \|@LB@5'-GCG...GCCAU C GGAUU...GGU-3'@LB@ GUUGCGCCGUAA UUAU@LB@ \|:\|\|\|\|\|\|\|\|\|\| \|\|\|\|@LB@ CGACGCGGCAUU AAUA@LB@3'-UUU...AGAAC U AUACU...GUG-5'@LB@ \| \|@LB@ 61 43@LB@@LB@@LB@Energy -9.30 kcal/mol@LB@Hybridization Energy -22.6138 kcal/mol@LB@Unfolding Energy - mRNA 6.57247 kcal/mol@LB@Unfolding Energy - sRNA 6.74006 kcal/mol@LB@@LB@Position - mRNA 90 -- 106@LB@Position - sRNA 44 -- 60@LB@Position Seed - mRNA 95 -- 101@LB@Position Seed - sRNA 49 -- 55@LB@
129	0.007194	0.1964	b3401	hslO	-8.25	0.287762	98 -- 107	1 -- 10	heat shock protein Hsp33		_____________97_________108\n_____________\|__________\|\n5'-GCC...UUAAC__U_______CCGGA...AGA-3'\n______________CA_GCCGCAC\n______________\|\|_\|\|\|\|\|\|\|\n______________GU_CGGCGUG\n3'-UUU...AAAAA__C_______-5'\n_____________\|_________\|\n_____________11________1\n	101 -- 107	1 -- 7	-14.7655 kcal/mol	1.13179 kcal/mol	5.44172 kcal/mol	97 108@LB@ \| \|@LB@5'-GCC...UUAAC U CCGGA...AGA-3'@LB@ CA GCCGCAC@LB@ \|\| \|\|\|\|\|\|\|@LB@ GU CGGCGUG@LB@3'-UUU...AAAAA C -5'@LB@ \| \|@LB@ 11 1@LB@@LB@@LB@Energy -8.25 kcal/mol@LB@Hybridization Energy -14.7655 kcal/mol@LB@Unfolding Energy - mRNA 1.13179 kcal/mol@LB@Unfolding Energy - sRNA 5.44172 kcal/mol@LB@@LB@Position - mRNA 98 -- 107@LB@Position - sRNA 1 -- 10@LB@Position Seed - mRNA 101 -- 107@LB@Position Seed - sRNA 1 -- 7@LB@
130	0.007275	0.1966	b1638	pdxH	-11.04	0.087983	237 -- 249	47 -- 59	pyridoxine 5'-phosphate oxidase		_____________236___________250\n_____________\|_____________\|\n5'-UCU...CGCAU_____________ACACC...CCC-3'\n______________CUGCGCCGUGAAU\n______________\|\|\|\|\|\|\|\|\|:\|\|\|\n______________GACGCGGCAUUUA\n3'-UUU...GAACC_____________AUAAU...GUG-5'\n_____________\|_____________\|\n_____________60____________46\n	243 -- 249	47 -- 53	-22.1181 kcal/mol	4.88729 kcal/mol	6.18672 kcal/mol	236 250@LB@ \| \|@LB@5'-UCU...CGCAU ACACC...CCC-3'@LB@ CUGCGCCGUGAAU@LB@ \|\|\|\|\|\|\|\|\|:\|\|\|@LB@ GACGCGGCAUUUA@LB@3'-UUU...GAACC AUAAU...GUG-5'@LB@ \| \|@LB@ 60 46@LB@@LB@@LB@Energy -11.04 kcal/mol@LB@Hybridization Energy -22.1181 kcal/mol@LB@Unfolding Energy - mRNA 4.88729 kcal/mol@LB@Unfolding Energy - sRNA 6.18672 kcal/mol@LB@@LB@Position - mRNA 237 -- 249@LB@Position - sRNA 47 -- 59@LB@Position Seed - mRNA 243 -- 249@LB@Position Seed - sRNA 47 -- 53@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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