Input and runtime details for job 6527768 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6527768 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:24:51 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:25:20 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 20:38:58 CET 2018
	Post-Processing Finished		@ Fri Feb 16 20:39:05 CET 2018
	Job Completed		@ Fri Feb 16 20:39:38 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6527768&toolName=CopraRNA ( 30 days expiry )

Description of the job

MicA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
185	0.01831	0.3104	b3164	pnp	-7.42	0.310961	131 -- 137	8 -- 14	polynucleotide phosphorylase/polyadenylase	_____________130_____138\n_____________\|_______\|\n5'-AGC...GCGAG_______AGAAG...UUA-3'\n______________GAUGCGC\n______________:\|\|\|\|\|\|\n______________UUACGCG\n3'-UUU...AUUGU_______CAGAAAG-5'\n_____________\|_______\|\n_____________15______7\n	131 -- 137	8 -- 14	-10.1419 kcal/mol	2.24272 kcal/mol	0.481462 kcal/mol	130 138@LB@ \| \|@LB@5'-AGC...GCGAG AGAAG...UUA-3'@LB@ GAUGCGC@LB@ :\|\|\|\|\|\|@LB@ UUACGCG@LB@3'-UUU...AUUGU CAGAAAG-5'@LB@ \| \|@LB@ 15 7@LB@@LB@@LB@Energy -7.42 kcal/mol@LB@Hybridization Energy -10.1419 kcal/mol@LB@Unfolding Energy - mRNA 2.24272 kcal/mol@LB@Unfolding Energy - sRNA 0.481462 kcal/mol@LB@@LB@Position - mRNA 131 -- 137@LB@Position - sRNA 8 -- 14@LB@Position Seed - mRNA 131 -- 137@LB@Position Seed - sRNA 8 -- 14@LB@
162	0.01506	0.3104	b0871	poxB	-6.02	0.481603	228 -- 255	23 -- 53	pyruvate dehydrogenase thiamine triphosphate-binding FAD-binding	_____________227_______________________________256\n_____________\|_________________________________\|\n5'-CCG...AUAUC_____________________CGG_________AACGC...GUG-3'\n______________GCCAAAA___CA_CUC_GAAU___CAGGGGUGA\n______________\|\|\|\|\|\|\|___\|\|_\|\|\|_\|\|\|\|___\|\|\|\|\|:\|\|\|\n______________CGGUUUU___GU_GAG_CUUA___GUCCCUACU\n3'-UUU...CUCAC_______AAA__A___A____A___________ACUAU...AAG-5'\n_____________\|_________________________________\|\n_____________54________________________________22\n	249 -- 255	23 -- 29	-28.0165 kcal/mol	6.84512 kcal/mol	15.1535 kcal/mol	227 256@LB@ \| \|@LB@5'-CCG...AUAUC CGG AACGC...GUG-3'@LB@ GCCAAAA CA CUC GAAU CAGGGGUGA@LB@ \|\|\|\|\|\|\| \|\| \|\|\| \|\|\|\| \|\|\|\|\|:\|\|\|@LB@ CGGUUUU GU GAG CUUA GUCCCUACU@LB@3'-UUU...CUCAC AAA A A A ACUAU...AAG-5'@LB@ \| \|@LB@ 54 22@LB@@LB@@LB@Energy -6.02 kcal/mol@LB@Hybridization Energy -28.0165 kcal/mol@LB@Unfolding Energy - mRNA 6.84512 kcal/mol@LB@Unfolding Energy - sRNA 15.1535 kcal/mol@LB@@LB@Position - mRNA 228 -- 255@LB@Position - sRNA 23 -- 53@LB@Position Seed - mRNA 249 -- 255@LB@Position Seed - sRNA 23 -- 29@LB@
147	0.01411	0.3104	b0441	ppiD	-10.51	0.099467	81 -- 98	3 -- 21	periplasmic folding chaperone has an inactive PPIase domain	_____________80__________________99\n_____________\|___________________\|\n5'-GUA...UAUUC______UUUC_________GUGGG...ACC-3'\n______________GGUGAC_____UGCGUCUU\n______________\|:\|:\|\|_____:\|\|\|\|\|\|\|\n______________CUAUUG_____GCGCAGAA\n3'-UUU...UACUA______UUUAC________AG-5'\n_____________\|___________________\|\n_____________22__________________2\n	92 -- 98	3 -- 9	-17.0331 kcal/mol	5.27038 kcal/mol	1.257 kcal/mol	80 99@LB@ \| \|@LB@5'-GUA...UAUUC UUUC GUGGG...ACC-3'@LB@ GGUGAC UGCGUCUU@LB@ \|:\|:\|\| :\|\|\|\|\|\|\|@LB@ CUAUUG GCGCAGAA@LB@3'-UUU...UACUA UUUAC AG-5'@LB@ \| \|@LB@ 22 2@LB@@LB@@LB@Energy -10.51 kcal/mol@LB@Hybridization Energy -17.0331 kcal/mol@LB@Unfolding Energy - mRNA 5.27038 kcal/mol@LB@Unfolding Energy - sRNA 1.257 kcal/mol@LB@@LB@Position - mRNA 81 -- 98@LB@Position - sRNA 3 -- 21@LB@Position Seed - mRNA 92 -- 98@LB@Position Seed - sRNA 3 -- 9@LB@
9	0.000195	0.07335	b1830	prc	-15.09	0.015143	131 -- 161	7 -- 38	carboxy-terminal protease for penicillin-binding protein 3	_____________130______________________________162\n_____________\|________________________________\|\n5'-CGA...UCCAG___________U___UCUU____U________CAGAA...CGG-3'\n______________CUGAAUUCGGG_AUG____UGAU__GUGCGCG\n______________\|\|\|\|\|\|\|\|:\|\|_\|\|\|____\|:\|:__:\|\|\|\|\|\|\n______________GACUUAAGUCC_UAC____AUUG__UACGCGC\n3'-UUU...GUAGA___________C___UACU____UU_______AGAAAG-5'\n_____________\|________________________________\|\n_____________39_______________________________6\n	155 -- 161	7 -- 13	-31.7028 kcal/mol	7.25259 kcal/mol	9.35859 kcal/mol	130 162@LB@ \| \|@LB@5'-CGA...UCCAG U UCUU U CAGAA...CGG-3'@LB@ CUGAAUUCGGG AUG UGAU GUGCGCG@LB@ \|\|\|\|\|\|\|\|:\|\| \|\|\| \|:\|: :\|\|\|\|\|\|@LB@ GACUUAAGUCC UAC AUUG UACGCGC@LB@3'-UUU...GUAGA C UACU UU AGAAAG-5'@LB@ \| \|@LB@ 39 6@LB@@LB@@LB@Energy -15.09 kcal/mol@LB@Hybridization Energy -31.7028 kcal/mol@LB@Unfolding Energy - mRNA 7.25259 kcal/mol@LB@Unfolding Energy - sRNA 9.35859 kcal/mol@LB@@LB@Position - mRNA 131 -- 161@LB@Position - sRNA 7 -- 38@LB@Position Seed - mRNA 155 -- 161@LB@Position Seed - sRNA 7 -- 13@LB@
97	0.009805	0.3104	b1306	pspC	-10.74	0.090670	128 -- 139	7 -- 18	psp operon transcription co-activator	_____________127__________140\n_____________\|____________\|\n5'-CCG...AGCAA____________AACGU...CGG-3'\n______________AACGGAUGCGCG\n______________\|\|\|::\|\|\|\|\|\|\|\n______________UUGUUUACGCGC\n3'-UUU...UACUA____________AGAAAG-5'\n_____________\|____________\|\n_____________19___________6\n	133 -- 139	7 -- 13	-18.5075 kcal/mol	6.55818 kcal/mol	1.20891 kcal/mol	127 140@LB@ \| \|@LB@5'-CCG...AGCAA AACGU...CGG-3'@LB@ AACGGAUGCGCG@LB@ \|\|\|::\|\|\|\|\|\|\|@LB@ UUGUUUACGCGC@LB@3'-UUU...UACUA AGAAAG-5'@LB@ \| \|@LB@ 19 6@LB@@LB@@LB@Energy -10.74 kcal/mol@LB@Hybridization Energy -18.5075 kcal/mol@LB@Unfolding Energy - mRNA 6.55818 kcal/mol@LB@Unfolding Energy - sRNA 1.20891 kcal/mol@LB@@LB@Position - mRNA 128 -- 139@LB@Position - sRNA 7 -- 18@LB@Position Seed - mRNA 133 -- 139@LB@Position Seed - sRNA 7 -- 13@LB@
159	0.01499	0.3104	b2476	purC	-11.66	0.062723	28 -- 49	4 -- 22	phosphoribosylaminoimidazole-succinocarboxamide synthetase	_____________27_____________________50\n_____________\|______________________\|\n5'-GGA...UGAUA_____C____AC__________GCAGG...CAG-3'\n______________UGAUA_GCAA__GUGUGCGUCU\n______________\|\|\|\|\|_:\|\|\|__:\|\|:\|\|\|\|\|\|\n______________ACUAU_UGUU__UACGCGCAGA\n3'-UUU...CUACU______________________AAG-5'\n_____________\|______________________\|\n_____________23_____________________3\n	43 -- 49	4 -- 10	-20.6907 kcal/mol	7.77162 kcal/mol	1.2572 kcal/mol	27 50@LB@ \| \|@LB@5'-GGA...UGAUA C AC GCAGG...CAG-3'@LB@ UGAUA GCAA GUGUGCGUCU@LB@ \|\|\|\|\| :\|\|\| :\|\|:\|\|\|\|\|\|@LB@ ACUAU UGUU UACGCGCAGA@LB@3'-UUU...CUACU AAG-5'@LB@ \| \|@LB@ 23 3@LB@@LB@@LB@Energy -11.66 kcal/mol@LB@Hybridization Energy -20.6907 kcal/mol@LB@Unfolding Energy - mRNA 7.77162 kcal/mol@LB@Unfolding Energy - sRNA 1.2572 kcal/mol@LB@@LB@Position - mRNA 28 -- 49@LB@Position - sRNA 4 -- 22@LB@Position Seed - mRNA 43 -- 49@LB@Position Seed - sRNA 4 -- 10@LB@
83	0.007683	0.3104	b4244	pyrI	-7.62	0.290375	210 -- 222	10 -- 21	aspartate carbamoyltransferase regulatory subunit	_____________209___________223\n_____________\|_____________\|\n5'-UUC...CACAC__________U__AGGUU...UCA-3'\n______________GAUAAUAAAU_GC\n______________\|\|\|\|\|:\|\|\|\|_\|\|\n______________CUAUUGUUUA_CG\n3'-UUU...UACUA_____________CGCAG...AAG-5'\n_____________\|_____________\|\n_____________22____________9\n	213 -- 219	12 -- 18	-11.1174 kcal/mol	2.26673 kcal/mol	1.23076 kcal/mol	209 223@LB@ \| \|@LB@5'-UUC...CACAC U AGGUU...UCA-3'@LB@ GAUAAUAAAU GC@LB@ \|\|\|\|\|:\|\|\|\| \|\|@LB@ CUAUUGUUUA CG@LB@3'-UUU...UACUA CGCAG...AAG-5'@LB@ \| \|@LB@ 22 9@LB@@LB@@LB@Energy -7.62 kcal/mol@LB@Hybridization Energy -11.1174 kcal/mol@LB@Unfolding Energy - mRNA 2.26673 kcal/mol@LB@Unfolding Energy - sRNA 1.23076 kcal/mol@LB@@LB@Position - mRNA 210 -- 222@LB@Position - sRNA 10 -- 21@LB@Position Seed - mRNA 213 -- 219@LB@Position Seed - sRNA 12 -- 18@LB@
129	0.01261	0.3104	b3025	qseB	-10.92	0.084347	141 -- 152	5 -- 17	quorum sensing DNA-binding response regulator in two-component regulatory system with QseC	_____________140___________153\n_____________\|_____________\|\n5'-GUG...GCUGU_____________ACAAC...GUC-3'\n______________ACAGA_GCGCGUU\n______________\|\|\|:\|_\|\|\|\|\|\|:\n______________UGUUU_CGCGCAG\n3'-UUU...ACUAU_____A_______AAAG-5'\n_____________\|_____________\|\n_____________18____________4\n	146 -- 152	5 -- 11	-15.7823 kcal/mol	3.63703 kcal/mol	1.22241 kcal/mol	140 153@LB@ \| \|@LB@5'-GUG...GCUGU ACAAC...GUC-3'@LB@ ACAGA GCGCGUU@LB@ \|\|\|:\| \|\|\|\|\|\|:@LB@ UGUUU CGCGCAG@LB@3'-UUU...ACUAU A AAAG-5'@LB@ \| \|@LB@ 18 4@LB@@LB@@LB@Energy -10.92 kcal/mol@LB@Hybridization Energy -15.7823 kcal/mol@LB@Unfolding Energy - mRNA 3.63703 kcal/mol@LB@Unfolding Energy - sRNA 1.22241 kcal/mol@LB@@LB@Position - mRNA 141 -- 152@LB@Position - sRNA 5 -- 17@LB@Position Seed - mRNA 146 -- 152@LB@Position Seed - sRNA 5 -- 11@LB@
88	0.008928	0.3104	b0405	queA	-8.89	0.184414	201 -- 209	5 -- 13	S-adenosylmethionine:tRNA ribosyltransferase-isomerase	_____________200_______210\n_____________\|_________\|\n5'-GGG...GUGUC_________ACCGA...GGC-3'\n______________AUGCGCGUU\n______________\|\|\|\|\|\|\|\|:\n______________UACGCGCAG\n3'-UUU...UUGUU_________AAAG-5'\n_____________\|_________\|\n_____________14________4\n	203 -- 209	5 -- 11	-14.1161 kcal/mol	4.07268 kcal/mol	1.15134 kcal/mol	200 210@LB@ \| \|@LB@5'-GGG...GUGUC ACCGA...GGC-3'@LB@ AUGCGCGUU@LB@ \|\|\|\|\|\|\|\|:@LB@ UACGCGCAG@LB@3'-UUU...UUGUU AAAG-5'@LB@ \| \|@LB@ 14 4@LB@@LB@@LB@Energy -8.89 kcal/mol@LB@Hybridization Energy -14.1161 kcal/mol@LB@Unfolding Energy - mRNA 4.07268 kcal/mol@LB@Unfolding Energy - sRNA 1.15134 kcal/mol@LB@@LB@Position - mRNA 201 -- 209@LB@Position - sRNA 5 -- 13@LB@Position Seed - mRNA 203 -- 209@LB@Position Seed - sRNA 5 -- 11@LB@
190	0.01867	0.3104	b0196	rcsF	-10.27	0.109081	201 -- 211	2 -- 13	putative outer membrane protein	_____________200__________212\n_____________\|____________\|\n5'-AAU...AUACU____________ACCGA...AGC-3'\n______________AUGCGUG_CUUU\n______________\|\|\|\|\|:\|_\|\|\|\|\n______________UACGCGC_GAAA\n3'-UUU...UUGUU_______A____G-5'\n_____________\|____________\|\n_____________14___________1\n	201 -- 207	7 -- 13	-12.8527 kcal/mol	1.40833 kcal/mol	1.17421 kcal/mol	200 212@LB@ \| \|@LB@5'-AAU...AUACU ACCGA...AGC-3'@LB@ AUGCGUG CUUU@LB@ \|\|\|\|\|:\| \|\|\|\|@LB@ UACGCGC GAAA@LB@3'-UUU...UUGUU A G-5'@LB@ \| \|@LB@ 14 1@LB@@LB@@LB@Energy -10.27 kcal/mol@LB@Hybridization Energy -12.8527 kcal/mol@LB@Unfolding Energy - mRNA 1.40833 kcal/mol@LB@Unfolding Energy - sRNA 1.17421 kcal/mol@LB@@LB@Position - mRNA 201 -- 211@LB@Position - sRNA 2 -- 13@LB@Position Seed - mRNA 201 -- 207@LB@Position Seed - sRNA 7 -- 13@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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usability assessment

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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