| 111 |
0.004819 |
0.1671 |
bsu32120 |
yuzG |
-7.22 |
0.752372 |
165 -- 182 |
149 -- 166 |
Evidence 5: No homology to any previously reported sequences |
|
_____________164______________________________________183\n_____________|________________________________________|\n5'-UAG...UCUUU_____A_G_ACA__G__AAG__A________GAUG_____AAACG...UGG-3'\n______________AUGGC_U_G___UG_GA____G_GCGAAUAA____GCCCA\n______________|||||_|_|___||_:|____|_||:||:||____:||||\n______________UACCG_A_C___AC_UU____C_CGUUUGUU____UGGGU\n3'-AUU...UUACU_____A_G_A____G__AGAA__________AGCA_____AGAGA...CAA-5'\n_____________|________________________________________|\n_____________167______________________________________148\n |
192 -- 198 |
62 -- 68 |
-24.2214 kcal/mol |
8.24153 kcal/mol |
10.1588 kcal/mol |
164 183@LB@ | |@LB@5'-UAG...UCUUU A G ACA G AAG A GAUG AAACG...UGG-3'@LB@ AUGGC U G UG GA G GCGAAUAA GCCCA@LB@ ||||| | | || :| | ||:||:|| :||||@LB@ UACCG A C AC UU C CGUUUGUU UGGGU@LB@3'-AUU...UUACU A G A G AGAA AGCA AGAGA...CAA-5'@LB@ | |@LB@ 167 148@LB@@LB@@LB@Energy -7.22 kcal/mol@LB@Hybridization Energy -24.2214 kcal/mol@LB@Unfolding Energy - mRNA 8.24153 kcal/mol@LB@Unfolding Energy - sRNA 10.1588 kcal/mol@LB@@LB@Position - mRNA 165 -- 182@LB@Position - sRNA 149 -- 166@LB@Position Seed - mRNA 192 -- 198@LB@Position Seed - sRNA 62 -- 68@LB@ |
| 112 |
0.00483 |
0.1671 |
bsu26010 |
yqbQ |
-18.75 |
0.005285 |
180 -- 239 |
82 -- 139 |
Evidence 4: Homologs of previously reported genes of unknown function |
bsu12700 |
_____________179______________________________________________________________240\n_____________|________________________________________________________________|\n5'-CUA...AAAUA_______________G______ACA__AUAGA___________A______AAACG_________GAGCU...UUA-3'\n______________CCGCAGUAAG___CA_GUGAUG___UG_____AC_UUUUCGUC_UUAAAG_____GAAUGGCUU\n______________||||||:|||___||_:|:|||___||_____||_||:||:||_|:||||_____|||||||||\n______________GGCGUCGUUC___GU_UAUUAC___AC_____UG_AAGAGUAG_AGUUUC_____CUUACCGAA\n3'-AUU...UAACA__________AAG__A______A____AG_____A________A______AUUA__________GCAAC...CAA-5'\n_____________|________________________________________________________________|\n_____________140______________________________________________________________81\n |
233 -- 239 |
82 -- 88 |
-46.2512 kcal/mol |
11.9879 kcal/mol |
18.3017 kcal/mol |
179 240@LB@ | |@LB@5'-CUA...AAAUA G ACA AUAGA A AAACG GAGCU...UUA-3'@LB@ CCGCAGUAAG CA GUGAUG UG AC UUUUCGUC UUAAAG GAAUGGCUU@LB@ ||||||:||| || :|:||| || || ||:||:|| |:|||| |||||||||@LB@ GGCGUCGUUC GU UAUUAC AC UG AAGAGUAG AGUUUC CUUACCGAA@LB@3'-AUU...UAACA AAG A A AG A A AUUA GCAAC...CAA-5'@LB@ | |@LB@ 140 81@LB@@LB@@LB@Energy -18.75 kcal/mol@LB@Hybridization Energy -46.2512 kcal/mol@LB@Unfolding Energy - mRNA 11.9879 kcal/mol@LB@Unfolding Energy - sRNA 18.3017 kcal/mol@LB@@LB@Position - mRNA 180 -- 239@LB@Position - sRNA 82 -- 139@LB@Position Seed - mRNA 233 -- 239@LB@Position Seed - sRNA 82 -- 88@LB@ |
| 113 |
0.005141 |
0.1741 |
bsu36080 |
ywrF |
-10.09 |
0.302867 |
265 -- 274 |
38 -- 47 |
Evidence 4: Homologs of previously reported genes of unknown function |
|
_____________264________275\n_____________|__________|\n5'-UGG...UACCG__________CCCCA...UUU-3'\n______________UUAUUCCCCG\n______________|||||||||:\n______________AAUAAGGGGU\n3'-AUU...UAGAG__________AAAGA...CAA-5'\n_____________|__________|\n_____________48_________37\n |
268 -- 274 |
38 -- 44 |
-13.6226 kcal/mol |
1.45475 kcal/mol |
3.86656 kcal/mol |
264 275@LB@ | |@LB@5'-UGG...UACCG CCCCA...UUU-3'@LB@ UUAUUCCCCG@LB@ |||||||||:@LB@ AAUAAGGGGU@LB@3'-AUU...UAGAG AAAGA...CAA-5'@LB@ | |@LB@ 48 37@LB@@LB@@LB@Energy -10.09 kcal/mol@LB@Hybridization Energy -13.6226 kcal/mol@LB@Unfolding Energy - mRNA 1.45475 kcal/mol@LB@Unfolding Energy - sRNA 3.86656 kcal/mol@LB@@LB@Position - mRNA 265 -- 274@LB@Position - sRNA 38 -- 47@LB@Position Seed - mRNA 268 -- 274@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 114 |
0.005219 |
0.1741 |
bsu18630 |
exlX |
-14.36 |
0.045196 |
231 -- 263 |
38 -- 88 |
expansin-YoaJ |
|
_____________230________________________________________________________264\n_____________|__________________________________________________________|\n5'-GGG...AGAUC_______CA_______GUA________UU________U____________________CAAGC...GUU-3'\n______________AUGAGUG__UUUGUUG___UGGUUUUG___GACGAUC___UCUGUUUU____UCCCCG\n______________||||:||__||:||||___:::|:|||___:||:|||___:|::|:|:____|||||:\n______________UACUUAC__AAGCAAC___GUUAGAAC___UUGUUAG___GGGUAGAG____AGGGGU\n3'-AUU...UUCAU_______CG__________________CGU_______CAU________AAUA______AAAGA...CAA-5'\n_____________|__________________________________________________________|\n_____________89_________________________________________________________37\n |
242 -- 248 |
59 -- 65 |
-36.6264 kcal/mol |
9.40493 kcal/mol |
14.3562 kcal/mol |
230 264@LB@ | |@LB@5'-GGG...AGAUC CA GUA UU U CAAGC...GUU-3'@LB@ AUGAGUG UUUGUUG UGGUUUUG GACGAUC UCUGUUUU UCCCCG@LB@ ||||:|| ||:|||| :::|:||| :||:||| :|::|:|: |||||:@LB@ UACUUAC AAGCAAC GUUAGAAC UUGUUAG GGGUAGAG AGGGGU@LB@3'-AUU...UUCAU CG CGU CAU AAUA AAAGA...CAA-5'@LB@ | |@LB@ 89 37@LB@@LB@@LB@Energy -14.36 kcal/mol@LB@Hybridization Energy -36.6264 kcal/mol@LB@Unfolding Energy - mRNA 9.40493 kcal/mol@LB@Unfolding Energy - sRNA 14.3562 kcal/mol@LB@@LB@Position - mRNA 231 -- 263@LB@Position - sRNA 38 -- 88@LB@Position Seed - mRNA 242 -- 248@LB@Position Seed - sRNA 59 -- 65@LB@ |
| 115 |
0.005245 |
0.1741 |
bsu07690 |
yflG |
-14.30 |
0.046489 |
29 -- 56 |
32 -- 57 |
methionine aminopeptidase 2 |
|
_____________28___________________________57\n_____________|____________________________|\n5'-UUC...AAGCA________CCC__C__A____U______CAAAU...GCA-3'\n______________UACCCAUC___CU_AU_CCCU_UUUUUU\n______________||||||||___||_||_|||:_|||:||\n______________AUGGGUAG___GA_UA_GGGG_AAAGAA\n3'-AUU...UUAGC________A____A__A____U______ACUUU...CAA-5'\n_____________|____________________________|\n_____________58___________________________31\n |
30 -- 36 |
50 -- 56 |
-21.2542 kcal/mol |
0.575621 kcal/mol |
9.1585 kcal/mol |
28 57@LB@ | |@LB@5'-UUC...AAGCA CCC C A U CAAAU...GCA-3'@LB@ UACCCAUC CU AU CCCU UUUUUU@LB@ |||||||| || || |||: |||:||@LB@ AUGGGUAG GA UA GGGG AAAGAA@LB@3'-AUU...UUAGC A A A U ACUUU...CAA-5'@LB@ | |@LB@ 58 31@LB@@LB@@LB@Energy -14.30 kcal/mol@LB@Hybridization Energy -21.2542 kcal/mol@LB@Unfolding Energy - mRNA 0.575621 kcal/mol@LB@Unfolding Energy - sRNA 9.1585 kcal/mol@LB@@LB@Position - mRNA 29 -- 56@LB@Position - sRNA 32 -- 57@LB@Position Seed - mRNA 30 -- 36@LB@Position Seed - sRNA 50 -- 56@LB@ |
| 116 |
0.005334 |
0.1741 |
bsu30510 |
ytpA |
-10.58 |
0.247824 |
123 -- 177 |
35 -- 87 |
phospholipase YtpA |
|
_____________122______________________________________________________178\n_____________|________________________________________________________|\n5'-AUU...AUAUC______G____C_UUAUAAG_______C__AAG___________CU__________AAAUU...GGC-3'\n______________UGGCUU_UGUU_C_______GGUAGAU_AU___AUUCAUC_CUU__UUCUCUAUUU\n______________||||||_:|||_|_______||:|:|:_||___|::||||_|||__|||:|:||||\n______________ACCGAA_GCAA_G_______CCGUUUG_UA___UGGGUAG_GAA__AAGGGGUAAA\n3'-AUU...UACUU___________C_UUAGAA________U__GCA_______A___U___________GAAAC...CAA-5'\n_____________|________________________________________________________|\n_____________88_______________________________________________________34\n |
171 -- 177 |
35 -- 41 |
-35.4104 kcal/mol |
13.324 kcal/mol |
14.1583 kcal/mol |
122 178@LB@ | |@LB@5'-AUU...AUAUC G C UUAUAAG C AAG CU AAAUU...GGC-3'@LB@ UGGCUU UGUU C GGUAGAU AU AUUCAUC CUU UUCUCUAUUU@LB@ |||||| :||| | ||:|:|: || |::|||| ||| |||:|:||||@LB@ ACCGAA GCAA G CCGUUUG UA UGGGUAG GAA AAGGGGUAAA@LB@3'-AUU...UACUU C UUAGAA U GCA A U GAAAC...CAA-5'@LB@ | |@LB@ 88 34@LB@@LB@@LB@Energy -10.58 kcal/mol@LB@Hybridization Energy -35.4104 kcal/mol@LB@Unfolding Energy - mRNA 13.324 kcal/mol@LB@Unfolding Energy - sRNA 14.1583 kcal/mol@LB@@LB@Position - mRNA 123 -- 177@LB@Position - sRNA 35 -- 87@LB@Position Seed - mRNA 171 -- 177@LB@Position Seed - sRNA 35 -- 41@LB@ |
| 117 |
0.005342 |
0.1741 |
bsu33050 |
gerAA |
-9.34 |
0.402150 |
52 -- 63 |
38 -- 49 |
spore germination protein A1 |
|
_____________51___________64\n_____________|____________|\n5'-UAA...UUUCU____________GCUAG...UGC-3'\n______________UCUUGUUUUCCA\n______________||||:||::|||\n______________AGAAUAAGGGGU\n3'-AUU...GGUAG____________AAAGA...CAA-5'\n_____________|____________|\n_____________50___________37\n |
57 -- 63 |
38 -- 44 |
-12.8318 kcal/mol |
1.31908 kcal/mol |
4.13482 kcal/mol |
51 64@LB@ | |@LB@5'-UAA...UUUCU GCUAG...UGC-3'@LB@ UCUUGUUUUCCA@LB@ ||||:||::|||@LB@ AGAAUAAGGGGU@LB@3'-AUU...GGUAG AAAGA...CAA-5'@LB@ | |@LB@ 50 37@LB@@LB@@LB@Energy -9.34 kcal/mol@LB@Hybridization Energy -12.8318 kcal/mol@LB@Unfolding Energy - mRNA 1.31908 kcal/mol@LB@Unfolding Energy - sRNA 4.13482 kcal/mol@LB@@LB@Position - mRNA 52 -- 63@LB@Position - sRNA 38 -- 49@LB@Position Seed - mRNA 57 -- 63@LB@Position Seed - sRNA 38 -- 44@LB@ |
| 118 |
0.005364 |
0.1741 |
bsu21980 |
ypdP |
-14.81 |
0.036422 |
190 -- 236 |
72 -- 112 |
'Evidence 3: Function proposed based on presence of conserved amino acid motif structural feature or limited homology; PubMedId: 15849754 16850406; Product type pm: membrane component' |
|
_____________189_______________________________________________237\n_____________|_________________________________________________|\n5'-AAC...AGGAA_____________GUUUAAUAAUUC__UU___________U________AUUCA...GGA-3'\n______________GCUUUUUCAUCUU____________GU__UGGAU__CUUU_UUGCGAUC\n______________:||||:|||||||____________||__||:||__|||:_||||:|||\n______________UGAAAGAGUAGAA____________CA__ACUUA__GAAG_AACGUUAG\n3'-AUU...AACAG_____________GUUU__________UU_____CC____C________AACCG...CAA-5'\n_____________|_________________________________________________|\n_____________113_______________________________________________71\n |
230 -- 236 |
72 -- 78 |
-29.9578 kcal/mol |
5.88063 kcal/mol |
12.3236 kcal/mol |
189 237@LB@ | |@LB@5'-AAC...AGGAA GUUUAAUAAUUC UU U AUUCA...GGA-3'@LB@ GCUUUUUCAUCUU GU UGGAU CUUU UUGCGAUC@LB@ :||||:||||||| || ||:|| |||: ||||:|||@LB@ UGAAAGAGUAGAA CA ACUUA GAAG AACGUUAG@LB@3'-AUU...AACAG GUUU UU CC C AACCG...CAA-5'@LB@ | |@LB@ 113 71@LB@@LB@@LB@Energy -14.81 kcal/mol@LB@Hybridization Energy -29.9578 kcal/mol@LB@Unfolding Energy - mRNA 5.88063 kcal/mol@LB@Unfolding Energy - sRNA 12.3236 kcal/mol@LB@@LB@Position - mRNA 190 -- 236@LB@Position - sRNA 72 -- 112@LB@Position Seed - mRNA 230 -- 236@LB@Position Seed - sRNA 72 -- 78@LB@ |
| 119 |
0.005369 |
0.1741 |
bsu33250 |
yvrL |
-8.38 |
0.555183 |
10 -- 36 |
76 -- 105 |
membrane-bound negative regulator YvrL |
|
_____________9__________37\n_____________|__________|\n5'-AUG...CCUUC__________UUGCG...AUA-3'\n______________AAAACGCUUA\n______________||||||||||\n______________UUUUGCGAAU\n3'-AUU...UAAAC__________UAAAG...CAA-5'\n_____________|__________|\n_____________106________75\n |
224 -- 230 |
13 -- 19 |
-10.8458 kcal/mol |
2.09283 kcal/mol |
3.31287 kcal/mol |
9 37@LB@ | |@LB@5'-AUG...CCUUC UUGCG...AUA-3'@LB@ AAAACGCUUA@LB@ ||||||||||@LB@ UUUUGCGAAU@LB@3'-AUU...UAAAC UAAAG...CAA-5'@LB@ | |@LB@ 106 75@LB@@LB@@LB@Energy -8.38 kcal/mol@LB@Hybridization Energy -10.8458 kcal/mol@LB@Unfolding Energy - mRNA 2.09283 kcal/mol@LB@Unfolding Energy - sRNA 3.31287 kcal/mol@LB@@LB@Position - mRNA 10 -- 36@LB@Position - sRNA 76 -- 105@LB@Position Seed - mRNA 224 -- 230@LB@Position Seed - sRNA 13 -- 19@LB@ |
| 120 |
0.005453 |
0.1741 |
bsu38750 |
cydB |
-16.17 |
0.018868 |
36 -- 121 |
37 -- 136 |
cytochrome d ubiquinol oxidase subunit 2 |
|
_____________35____________________________________________________________________________________________________________122\n_____________|_____________________________________________________________________________________________________________|\n5'-AAU...CGUAA___________________________C__AA_____GCA_____U______UAC___AU__________________________________U________A_____GAGAU...CCC-3'\n______________CAGCGGGUUCC________UUGUUAUU_UC__UCAUC___UUCGG_GUGAUG___AUG____________UCUUGGU__GCA__C_UGCUUGUC_UCUUGUUU_UCCGU\n______________||||::|||||________||||:|:|_||__|||||___|||::_||:|||___|||____________||||||:__:||__|_|:|:::||_||||:||:_:||:|\n______________GUCGUUCAAGG________AACAGUGA_AG__AGUAG___AAGUU_CAUUAC___UAC____________AGAACCG__UGU__G_AUGGGUAG_AGAAUAAG_GGGUA\n3'-AUU...CAGGC___________UAUAUUAC________A_________________U______U_____CGAAGCAACGUU_______UU___UA_C_______________________AAGAA...CAA-5'\n_____________|_____________________________________________________________________________________________________________|\n_____________137___________________________________________________________________________________________________________36\n |
109 -- 115 |
42 -- 48 |
-60.5971 kcal/mol |
23.6183 kcal/mol |
24.7809 kcal/mol |
35 122@LB@ | |@LB@5'-AAU...CGUAA C AA GCA U UAC AU U A GAGAU...CCC-3'@LB@ CAGCGGGUUCC UUGUUAUU UC UCAUC UUCGG GUGAUG AUG UCUUGGU GCA C UGCUUGUC UCUUGUUU UCCGU@LB@ ||||::||||| ||||:|:| || ||||| |||:: ||:||| ||| ||||||: :|| | |:|:::|| ||||:||: :||:|@LB@ GUCGUUCAAGG AACAGUGA AG AGUAG AAGUU CAUUAC UAC AGAACCG UGU G AUGGGUAG AGAAUAAG GGGUA@LB@3'-AUU...CAGGC UAUAUUAC A U U CGAAGCAACGUU UU UA C AAGAA...CAA-5'@LB@ | |@LB@ 137 36@LB@@LB@@LB@Energy -16.17 kcal/mol@LB@Hybridization Energy -60.5971 kcal/mol@LB@Unfolding Energy - mRNA 23.6183 kcal/mol@LB@Unfolding Energy - sRNA 24.7809 kcal/mol@LB@@LB@Position - mRNA 36 -- 121@LB@Position - sRNA 37 -- 136@LB@Position Seed - mRNA 109 -- 115@LB@Position Seed - sRNA 42 -- 48@LB@ |
