Input and runtime details for job 6793193 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 6793193 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:27:03 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:27:32 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 21:26:02 CET 2018
	Post-Processing Finished		@ Fri Feb 16 21:26:10 CET 2018
	Job Completed		@ Fri Feb 16 21:26:45 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=6793193&toolName=CopraRNA ( 30 days expiry )

Description of the job

RprA

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
101	0.004534	0.1538	b2079	baeR	-10.16	0.230535	155 -- 191	34 -- 71	response regulator in two-component regulatory system with BaeS	_____________154_______________________________________192\n_____________\|_________________________________________\|\n5'-CAG...GGUAA___U________A_____AC______G_AAC______UA__GAGAG...CGA-3'\n______________GCA_UACAGUAG___GUU____CGCU_G___GGGAUU__CA\n______________:\|\|_:\|\|\|\|:\|:___\|\|\|____\|:\|\|_:___\|\|\|\|\|\|__\|\|\n______________UGU_GUGUCGUU___CAA____GUGA_U___CCCUAA__GU\n3'-UUU...UCUGA____________AAG___CAAA____G_C________AG__ACGAA...GCA-5'\n_____________\|_________________________________________\|\n_____________72________________________________________33\n	160 -- 166	61 -- 67	-23.4603 kcal/mol	8.51419 kcal/mol	4.79237 kcal/mol	154 192@LB@ \| \|@LB@5'-CAG...GGUAA U A AC G AAC UA GAGAG...CGA-3'@LB@ GCA UACAGUAG GUU CGCU G GGGAUU CA@LB@ :\|\| :\|\|\|\|:\|: \|\|\| \|:\|\| : \|\|\|\|\|\| \|\|@LB@ UGU GUGUCGUU CAA GUGA U CCCUAA GU@LB@3'-UUU...UCUGA AAG CAAA G C AG ACGAA...GCA-5'@LB@ \| \|@LB@ 72 33@LB@@LB@@LB@Energy -10.16 kcal/mol@LB@Hybridization Energy -23.4603 kcal/mol@LB@Unfolding Energy - mRNA 8.51419 kcal/mol@LB@Unfolding Energy - sRNA 4.79237 kcal/mol@LB@@LB@Position - mRNA 155 -- 191@LB@Position - sRNA 34 -- 71@LB@Position Seed - mRNA 160 -- 166@LB@Position Seed - sRNA 61 -- 67@LB@
102	0.004593	0.1538	b0343	lacY	-16.22	0.009713	12 -- 55	39 -- 78	lactose permease	_____________11___________________________________________56\n_____________\|____________________________________________\|\n5'-UGC...ACGCG_____AG_________UG___A___ACG_____CA_AU______UGGUG...GGC-3'\n______________GAAGA__GCACAUGGC__AAU_UCG___GUUUC__U__GGGGAU\n______________:\|\|\|\|__:\|\|\|\|::\|\|__\|\|\|_\|\|\|___\|\|\|\|\|__\|__\|\|\|\|\|\|\n______________UUUCU__UGUGUGUCG__UUA_AGC___CAAAG__A__CCCCUA\n3'-UUU...ACCCG_____GA__________________AA______UG_GU______AAGGU...GCA-5'\n_____________\|____________________________________________\|\n_____________79___________________________________________38\n	21 -- 27	63 -- 69	-31.4377 kcal/mol	10.4352 kcal/mol	4.77966 kcal/mol	11 56@LB@ \| \|@LB@5'-UGC...ACGCG AG UG A ACG CA AU UGGUG...GGC-3'@LB@ GAAGA GCACAUGGC AAU UCG GUUUC U GGGGAU@LB@ :\|\|\|\| :\|\|\|\|::\|\| \|\|\| \|\|\| \|\|\|\|\| \| \|\|\|\|\|\|@LB@ UUUCU UGUGUGUCG UUA AGC CAAAG A CCCCUA@LB@3'-UUU...ACCCG GA AA UG GU AAGGU...GCA-5'@LB@ \| \|@LB@ 79 38@LB@@LB@@LB@Energy -16.22 kcal/mol@LB@Hybridization Energy -31.4377 kcal/mol@LB@Unfolding Energy - mRNA 10.4352 kcal/mol@LB@Unfolding Energy - sRNA 4.77966 kcal/mol@LB@@LB@Position - mRNA 12 -- 55@LB@Position - sRNA 39 -- 78@LB@Position Seed - mRNA 21 -- 27@LB@Position Seed - sRNA 63 -- 69@LB@
103	0.004595	0.1538	b3554	yiaF	-10.61	0.191588	8 -- 38	46 -- 75	barrier effect co-colonization resistance factor; DUF3053 family lipoprotein	_____________7_________________________________39\n_____________\|_________________________________\|\n____5'-AAUCUCU_______AAAAA_________GCACUA______UUUAA...AGC-3'\n______________GGCUACA_____GGCAA__CG______UCAUUU\n______________\|:\|\|\|\|\|_____:\|\|\|\|__\|\|______\|\|\|:\|:\n______________CUGAUGU_____UCGUU__GC______AGUGAG\n3'-UUU...CGUUU_______GUG_______AA__AACAA_______UCCCC...GCA-5'\n_____________\|_________________________________\|\n_____________76________________________________45\n	8 -- 14	69 -- 75	-17.9003 kcal/mol	3.5618 kcal/mol	3.72616 kcal/mol	7 39@LB@ \| \|@LB@ 5'-AAUCUCU AAAAA GCACUA UUUAA...AGC-3'@LB@ GGCUACA GGCAA CG UCAUUU@LB@ \|:\|\|\|\|\| :\|\|\|\| \|\| \|\|\|:\|:@LB@ CUGAUGU UCGUU GC AGUGAG@LB@3'-UUU...CGUUU GUG AA AACAA UCCCC...GCA-5'@LB@ \| \|@LB@ 76 45@LB@@LB@@LB@Energy -10.61 kcal/mol@LB@Hybridization Energy -17.9003 kcal/mol@LB@Unfolding Energy - mRNA 3.5618 kcal/mol@LB@Unfolding Energy - sRNA 3.72616 kcal/mol@LB@@LB@Position - mRNA 8 -- 38@LB@Position - sRNA 46 -- 75@LB@Position Seed - mRNA 8 -- 14@LB@Position Seed - sRNA 69 -- 75@LB@
104	0.004609	0.1538	b3405	ompR	-12.19	0.094658	154 -- 190	42 -- 77	response regulator in two-component regulatory system with EnvZ	_____________153___________________________________191\n_____________\|_____________________________________\|\n5'-AAU...UUUUU____A_______UUA___A______CGAAC___U___AGUAC...GCG-3'\n______________AAGA_UACACGC___CAA_UUGUUG_____CUU_GGG\n______________\|\|\|\|_\|\|\|\|\|:\|___\|\|\|_\|:\|\|\|\|_____\|\|:_\|\|\|\n______________UUCU_AUGUGUG___GUU_AGCAAC_____GAG_CCC\n3'-UUU...CCCGU____G_______UC____A______AAAGU___U___CUAAA...GCA-5'\n_____________\|_____________________________________\|\n_____________78____________________________________41\n	159 -- 165	66 -- 72	-25.6517 kcal/mol	8.69679 kcal/mol	4.7648 kcal/mol	153 191@LB@ \| \|@LB@5'-AAU...UUUUU A UUA A CGAAC U AGUAC...GCG-3'@LB@ AAGA UACACGC CAA UUGUUG CUU GGG@LB@ \|\|\|\| \|\|\|\|\|:\| \|\|\| \|:\|\|\|\| \|\|: \|\|\|@LB@ UUCU AUGUGUG GUU AGCAAC GAG CCC@LB@3'-UUU...CCCGU G UC A AAAGU U CUAAA...GCA-5'@LB@ \| \|@LB@ 78 41@LB@@LB@@LB@Energy -12.19 kcal/mol@LB@Hybridization Energy -25.6517 kcal/mol@LB@Unfolding Energy - mRNA 8.69679 kcal/mol@LB@Unfolding Energy - sRNA 4.7648 kcal/mol@LB@@LB@Position - mRNA 154 -- 190@LB@Position - sRNA 42 -- 77@LB@Position Seed - mRNA 159 -- 165@LB@Position Seed - sRNA 66 -- 72@LB@
105	0.004683	0.1538	b3349	slyD	-10.61	0.192215	166 -- 197	39 -- 73	FKBP-type peptidyl prolyl cis-trans isomerase (rotamase)	_____________165_________________________________198\n_____________\|___________________________________\|\n5'-UGU...ACAAU___________U____UUCCCAU________A___AUCAU...ACU-3'\n______________CUGCGC_CA_C_AUUC________GCUCAGG_GAU\n______________\|\|:\|:\|_\|\|_\|_\|\|\|\|________:\|\|\|\|\|\|_\|\|\|\n______________GAUGUG_GU_G_UAAG________UGAGUCC_CUA\n3'-UUU...UUUCU______U__C_U____CAACAAAG_______C___AAGGU...GCA-5'\n_____________\|___________________________________\|\n_____________74__________________________________38\n	187 -- 193	43 -- 49	-23.2642 kcal/mol	7.88699 kcal/mol	4.77295 kcal/mol	165 198@LB@ \| \|@LB@5'-UGU...ACAAU U UUCCCAU A AUCAU...ACU-3'@LB@ CUGCGC CA C AUUC GCUCAGG GAU@LB@ \|\|:\|:\| \|\| \| \|\|\|\| :\|\|\|\|\|\| \|\|\|@LB@ GAUGUG GU G UAAG UGAGUCC CUA@LB@3'-UUU...UUUCU U C U CAACAAAG C AAGGU...GCA-5'@LB@ \| \|@LB@ 74 38@LB@@LB@@LB@Energy -10.61 kcal/mol@LB@Hybridization Energy -23.2642 kcal/mol@LB@Unfolding Energy - mRNA 7.88699 kcal/mol@LB@Unfolding Energy - sRNA 4.77295 kcal/mol@LB@@LB@Position - mRNA 166 -- 197@LB@Position - sRNA 39 -- 73@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 43 -- 49@LB@
106	0.004704	0.1538	b0995	torR	-15.76	0.013096	243 -- 292	33 -- 70	response regulator in two-component regulatory system with TorS	_____________242_________________________________________________293\n_____________\|___________________________________________________\|\n5'-CCA...UUACC__G_________ACAAUCCUAC__________A__GGUAUACC________UACGGCGA-3'\n______________CA_GC_GCGAUU__________UUCACUCAGG_GG________GUUUCCGU\n______________\|\|_:\|_\|\|:\|\|\|__________\|\|\|\|\|\|\|\|\|\|_\|\|________:\|\|\|\|\|:\|\n______________GU_UG_CGUUAA__________AAGUGAGUCC_CC________UAAAGGUA\n3'-UUU...CUGAU__G__U______GCAACA_________________________________CGAAU...GCA-5'\n_____________\|___________________________________________________\|\n_____________71__________________________________________________32\n	286 -- 292	33 -- 39	-31.2367 kcal/mol	10.6838 kcal/mol	4.7924 kcal/mol	242 293@LB@ \| \|@LB@5'-CCA...UUACC G ACAAUCCUAC A GGUAUACC UACGGCGA-3'@LB@ CA GC GCGAUU UUCACUCAGG GG GUUUCCGU@LB@ \|\| :\| \|\|:\|\|\| \|\|\|\|\|\|\|\|\|\| \|\| :\|\|\|\|\|:\|@LB@ GU UG CGUUAA AAGUGAGUCC CC UAAAGGUA@LB@3'-UUU...CUGAU G U GCAACA CGAAU...GCA-5'@LB@ \| \|@LB@ 71 32@LB@@LB@@LB@Energy -15.76 kcal/mol@LB@Hybridization Energy -31.2367 kcal/mol@LB@Unfolding Energy - mRNA 10.6838 kcal/mol@LB@Unfolding Energy - sRNA 4.7924 kcal/mol@LB@@LB@Position - mRNA 243 -- 292@LB@Position - sRNA 33 -- 70@LB@Position Seed - mRNA 286 -- 292@LB@Position Seed - sRNA 33 -- 39@LB@
107	0.004745	0.1538	b2413	cysZ	-9.47	0.298503	222 -- 238	58 -- 74	sulfate transporter sulfite inhibited	_____________221_______________239\n_____________\|_________________\|\n5'-ACC...CAUCU__CC_____________UUACU...UUU-3'\n______________GC__CACGCAGCGGUUU\n______________:\|__\|\|\|:\|\|\|\|::\|\|:\n______________UG__GUGUGUCGUUAAG\n3'-UUU...GUUUC__AU_____________CAACA...GCA-5'\n_____________\|_________________\|\n_____________75________________57\n	232 -- 238	58 -- 64	-18.5455 kcal/mol	5.77464 kcal/mol	3.3008 kcal/mol	221 239@LB@ \| \|@LB@5'-ACC...CAUCU CC UUACU...UUU-3'@LB@ GC CACGCAGCGGUUU@LB@ :\| \|\|\|:\|\|\|\|::\|\|:@LB@ UG GUGUGUCGUUAAG@LB@3'-UUU...GUUUC AU CAACA...GCA-5'@LB@ \| \|@LB@ 75 57@LB@@LB@@LB@Energy -9.47 kcal/mol@LB@Hybridization Energy -18.5455 kcal/mol@LB@Unfolding Energy - mRNA 5.77464 kcal/mol@LB@Unfolding Energy - sRNA 3.3008 kcal/mol@LB@@LB@Position - mRNA 222 -- 238@LB@Position - sRNA 58 -- 74@LB@Position Seed - mRNA 232 -- 238@LB@Position Seed - sRNA 58 -- 64@LB@
108	0.004771	0.1538	b2594	rluD	-7.24	0.588975	189 -- 206	62 -- 78	23S rRNA pseudouridine(1911 1915 1917) synthase	_____________188________________207\n_____________\|__________________\|\n5'-CCC...ACGAA____C_G___________CAACG...GUU-3'\n______________GAAG_A_UAUAUAUGGCA\n______________:\|\|\|_\|_\|\|:\|:\|::\|\|\|\n______________UUUC_U_AUGUGUGUCGU\n3'-UUU...ACCCG______G___________UAAGC...GCA-5'\n_____________\|__________________\|\n_____________79_________________61\n	200 -- 206	62 -- 68	-13.9289 kcal/mol	3.43926 kcal/mol	3.2555 kcal/mol	188 207@LB@ \| \|@LB@5'-CCC...ACGAA C G CAACG...GUU-3'@LB@ GAAG A UAUAUAUGGCA@LB@ :\|\|\| \| \|\|:\|:\|::\|\|\|@LB@ UUUC U AUGUGUGUCGU@LB@3'-UUU...ACCCG G UAAGC...GCA-5'@LB@ \| \|@LB@ 79 61@LB@@LB@@LB@Energy -7.24 kcal/mol@LB@Hybridization Energy -13.9289 kcal/mol@LB@Unfolding Energy - mRNA 3.43926 kcal/mol@LB@Unfolding Energy - sRNA 3.2555 kcal/mol@LB@@LB@Position - mRNA 189 -- 206@LB@Position - sRNA 62 -- 78@LB@Position Seed - mRNA 200 -- 206@LB@Position Seed - sRNA 62 -- 68@LB@
109	0.004772	0.1538	b2580	ung	-9.28	0.319241	25 -- 55	36 -- 70	uracil-DNA-glycosylase	_____________24__________________________________56\n_____________\|___________________________________\|\n5'-UCU...AAAAC______U___GUA____G_____AAAU________AUUGU...CCG-3'\n______________CACACA_CAA_____UG__UCGC_____GGAUUUU\n______________\|\|\|\|\|\|_\|\|\|_____\|\|__\|\|:\|_____\|\|\|\|\|\|:\n______________GUGUGU_GUU_____AC__AGUG_____CCUAAAG\n3'-UUU...CUGAU______C___AAGCA__AA____AGUCC_______GUACG...GCA-5'\n_____________\|___________________________________\|\n_____________71__________________________________35\n	49 -- 55	36 -- 42	-19.0861 kcal/mol	5.04532 kcal/mol	4.7591 kcal/mol	24 56@LB@ \| \|@LB@5'-UCU...AAAAC U GUA G AAAU AUUGU...CCG-3'@LB@ CACACA CAA UG UCGC GGAUUUU@LB@ \|\|\|\|\|\| \|\|\| \|\| \|\|:\| \|\|\|\|\|\|:@LB@ GUGUGU GUU AC AGUG CCUAAAG@LB@3'-UUU...CUGAU C AAGCA AA AGUCC GUACG...GCA-5'@LB@ \| \|@LB@ 71 35@LB@@LB@@LB@Energy -9.28 kcal/mol@LB@Hybridization Energy -19.0861 kcal/mol@LB@Unfolding Energy - mRNA 5.04532 kcal/mol@LB@Unfolding Energy - sRNA 4.7591 kcal/mol@LB@@LB@Position - mRNA 25 -- 55@LB@Position - sRNA 36 -- 70@LB@Position Seed - mRNA 49 -- 55@LB@Position Seed - sRNA 36 -- 42@LB@
110	0.004806	0.1538	b1429	tehA	-12.16	0.096068	32 -- 62	44 -- 75	potassium-tellurite ethidium and proflavin transporter	_____________31_________________________________63\n_____________\|__________________________________\|\n5'-AAU...UUCCU___A__UAAA_________C___UC_________ACGAU...UUU-3'\n______________GAU_AC____GGC___UCG_UGU__UUCACUUAG\n______________\|\|:_\|\|____:\|\|___\|\|\|_\|\|\|__\|\|\|\|\|\|:\|\|\n______________CUG_UG____UCG___AGC_ACA__AAGUGAGUC\n3'-UUU...CGUUU___A__UGUG___UUA___A______________CCCUA...GCA-5'\n_____________\|__________________________________\|\n_____________76_________________________________43\n	56 -- 62	44 -- 50	-20.5577 kcal/mol	4.26011 kcal/mol	4.13839 kcal/mol	31 63@LB@ \| \|@LB@5'-AAU...UUCCU A UAAA C UC ACGAU...UUU-3'@LB@ GAU AC GGC UCG UGU UUCACUUAG@LB@ \|\|: \|\| :\|\| \|\|\| \|\|\| \|\|\|\|\|\|:\|\|@LB@ CUG UG UCG AGC ACA AAGUGAGUC@LB@3'-UUU...CGUUU A UGUG UUA A CCCUA...GCA-5'@LB@ \| \|@LB@ 76 43@LB@@LB@@LB@Energy -12.16 kcal/mol@LB@Hybridization Energy -20.5577 kcal/mol@LB@Unfolding Energy - mRNA 4.26011 kcal/mol@LB@Unfolding Energy - sRNA 4.13839 kcal/mol@LB@@LB@Position - mRNA 32 -- 62@LB@Position - sRNA 44 -- 75@LB@Position Seed - mRNA 56 -- 62@LB@Position Seed - sRNA 44 -- 50@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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service.

When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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