Input and runtime details for job 1786318 (precomputed example)

Sequence input

	sRNA sequences		[.fa]
	Putative target sequences (organism of interest)		[.fa]
	Extract sequences around		start codon
	nt up (1-300)		200
	nt down (1-300)		100

CopraRNA parameters

	Consensus prediction		off
	p-value combination		no
	p-value filtering (0=off)		0

IntaRNA parameters

	Target folding window size		150
	Target max. basepair distance		100

Job ID 1786318 (server version trunk)

	Job Submitted & Queued		@ Fri Feb 16 15:20:27 CET 2018
	CopraRNA Started		@ Fri Feb 16 15:20:56 CET 2018
	CopraRNA Finished & Post-Processing		@ Fri Feb 16 22:01:46 CET 2018
	Post-Processing Finished		@ Fri Feb 16 22:01:53 CET 2018
	Job Completed		@ Fri Feb 16 22:02:22 CET 2018

DIRECT ACCESS: http://rna.informatik.uni-freiburg.de/RetrieveResults.jsp?jobID=1786318&toolName=CopraRNA ( 30 days expiry )

Description of the job

ArcZ

Output download complete results [zip]

Downloadable files

	CopraRNA result table:		[csv]
	Functional enrichment file		[txt]
	Auxiliary enrichment file		[csv]
	mRNA regions plot		[png] [pdf] [ps]
	sRNA regions plot		[png] [pdf] [ps]
	Functional annotation chart		[html] [pdf]
	Phylogenetic conservation		[png] [pdf]
	16S rDNA tree		[txt] [svg]

mRNA region plot

sRNA region plot

Annot. chart

Conserved, identified interactions for NC_000913 Escherichia coli str. K-12 substr. MG1655

Select: 1-10 11-20 21-30 31-40 41-50 51-60 61-70 71-80 81-90 91-100 101-110 111-120 121-130 131-140 141-150 151-160 161-170 171-180 181-190 191-200 all

Sort by selecting a column name:

Rank	CopraRNA p-value	CopraRNA fdr value	Locus Tag	Gene Name	Energy [kcal/mol]	IntaRNA p-value	Position mRNA	Position sRNA	Annotation
137	0.007754	0.1993	b4024	lysC	-8.53	0.257132	131 -- 141	73 -- 85	lysine-sensitive aspartokinase 3	_____________130___________142\n_____________\|_____________\|\n5'-UGA...UCGCU_____________CGUCU...UGC-3'\n______________CUGCGCC__CACC\n______________\|\|\|\|\|\|\|__\|\|\|\|\n______________GACGCGG__GUGG\n3'-UUU...CUUAU_______UU____UCCCU...GUG-5'\n_____________\|_____________\|\n_____________86____________72\n	131 -- 137	79 -- 85	-18.3373 kcal/mol	2.85158 kcal/mol	6.95176 kcal/mol	130 142@LB@ \| \|@LB@5'-UGA...UCGCU CGUCU...UGC-3'@LB@ CUGCGCC CACC@LB@ \|\|\|\|\|\|\| \|\|\|\|@LB@ GACGCGG GUGG@LB@3'-UUU...CUUAU UU UCCCU...GUG-5'@LB@ \| \|@LB@ 86 72@LB@@LB@@LB@Energy -8.53 kcal/mol@LB@Hybridization Energy -18.3373 kcal/mol@LB@Unfolding Energy - mRNA 2.85158 kcal/mol@LB@Unfolding Energy - sRNA 6.95176 kcal/mol@LB@@LB@Position - mRNA 131 -- 141@LB@Position - sRNA 73 -- 85@LB@Position Seed - mRNA 131 -- 137@LB@Position Seed - sRNA 79 -- 85@LB@
66	0.003275	0.1727	b0394	mak	-8.12	0.303293	18 -- 92	5 -- 77	manno(fructo)kinase	_____________17_______________________________________________________________________________93\n_____________\|________________________________________________________________________________\|\n5'-GUU...CUUCU____G___C_______CGC____AA_A_G_____CC____AAUUUUUGA_______________GAUAA____CAC____UUGUC...GUG-3'\n______________GCAC_CAG_GAAAUCU___GGCU__G_C_GUAAA__AUUA_________AC_____CACAGCAU_____UUUC___GGCC\n______________:\|\|\|_\|\|\|_\|\|\|\|\|\|\|___\|\|\|\|__\|_\|_\|\|\|\|\|__\|\|\|\|_________\|\|_____\|\|\|\|\|\|\|:_____\|\|\|\|___\|\|\|\|\n______________UGUG_GUC_CUUUAGA___CCGA__C_G_CAUUU__UAAU_________UG_____GUGUCGUG_____AAAG___CCGG\n3'-UUU...GCGGU________C_______A______CG___G_____AA____ACUACUCAA__UUCCC________ACAA_____U______CGUG-5'\n_____________\|________________________________________________________________________________\|\n_____________78_______________________________________________________________________________4\n	70 -- 76	18 -- 24	-46.4183 kcal/mol	15.5654 kcal/mol	22.7286 kcal/mol	17 93@LB@ \| \|@LB@5'-GUU...CUUCU G C CGC AA A G CC AAUUUUUGA GAUAA CAC UUGUC...GUG-3'@LB@ GCAC CAG GAAAUCU GGCU G C GUAAA AUUA AC CACAGCAU UUUC GGCC@LB@ :\|\|\| \|\|\| \|\|\|\|\|\|\| \|\|\|\| \| \| \|\|\|\|\| \|\|\|\| \|\| \|\|\|\|\|\|\|: \|\|\|\| \|\|\|\|@LB@ UGUG GUC CUUUAGA CCGA C G CAUUU UAAU UG GUGUCGUG AAAG CCGG@LB@3'-UUU...GCGGU C A CG G AA ACUACUCAA UUCCC ACAA U CGUG-5'@LB@ \| \|@LB@ 78 4@LB@@LB@@LB@Energy -8.12 kcal/mol@LB@Hybridization Energy -46.4183 kcal/mol@LB@Unfolding Energy - mRNA 15.5654 kcal/mol@LB@Unfolding Energy - sRNA 22.7286 kcal/mol@LB@@LB@Position - mRNA 18 -- 92@LB@Position - sRNA 5 -- 77@LB@Position Seed - mRNA 70 -- 76@LB@Position Seed - sRNA 18 -- 24@LB@
143	0.008308	0.2033	b2049	cpsB	-11.81	0.062535	184 -- 242	25 -- 78	mannose-1-phosphate guanyltransferase	_____________183_______________________________________________________________243\n_____________\|_________________________________________________________________\|\n5'-ACA...UAAAU____U_______U____A_______A________CUCUAUCCA__________________U___UCCGG...GCC-3'\n______________GAUA_UCGGGGA_AAUU_UGGC_GC_GUCG_AAA_________GUUGUGAUG____GCAGG_GGC\n______________:\|:\|_:\|:\|\|\|\|_\|\|\|:_\|\|\|\|_\|\|_\|:\|\|_\|\|\|_________:\|\|:\|\|\|\|\|____:\|\|:\|_\|\|\|\n______________UUGU_GGUCCCU_UUAG_ACCG_CG_CGGC_UUU_________UAAUACUAC____UGUUC_CCG\n3'-UUU...CGCGG_________________A____A_______A___AA________________UCAA_________UGUCG...GUG-5'\n_____________\|_________________________________________________________________\|\n_____________79________________________________________________________________24\n	227 -- 233	37 -- 43	-41.0645 kcal/mol	15.3492 kcal/mol	13.8987 kcal/mol	183 243@LB@ \| \|@LB@5'-ACA...UAAAU U U A A CUCUAUCCA U UCCGG...GCC-3'@LB@ GAUA UCGGGGA AAUU UGGC GC GUCG AAA GUUGUGAUG GCAGG GGC@LB@ :\|:\| :\|:\|\|\|\| \|\|\|: \|\|\|\| \|\| \|:\|\| \|\|\| :\|\|:\|\|\|\|\| :\|\|:\| \|\|\|@LB@ UUGU GGUCCCU UUAG ACCG CG CGGC UUU UAAUACUAC UGUUC CCG@LB@3'-UUU...CGCGG A A A AA UCAA UGUCG...GUG-5'@LB@ \| \|@LB@ 79 24@LB@@LB@@LB@Energy -11.81 kcal/mol@LB@Hybridization Energy -41.0645 kcal/mol@LB@Unfolding Energy - mRNA 15.3492 kcal/mol@LB@Unfolding Energy - sRNA 13.8987 kcal/mol@LB@@LB@Position - mRNA 184 -- 242@LB@Position - sRNA 25 -- 78@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 37 -- 43@LB@
175	0.01031	0.2074	b1834	yebT	-10.32	0.121003	270 -- 282	51 -- 62	MCE domain protein	_____________269___________283\n_____________\|_____________\|\n5'-UCU...CUUUC_______U_____UCAUC...UUG-3'\n______________UGGCUGC_GCCGU\n______________\|\|\|\|\|\|\|_\|\|\|\|\|\n______________ACCGACG_CGGCA\n3'-UUU...UUAGA_____________UUUAA...GUG-5'\n_____________\|_____________\|\n_____________63____________50\n	270 -- 276	56 -- 62	-21.4836 kcal/mol	6.28034 kcal/mol	4.8826 kcal/mol	269 283@LB@ \| \|@LB@5'-UCU...CUUUC U UCAUC...UUG-3'@LB@ UGGCUGC GCCGU@LB@ \|\|\|\|\|\|\| \|\|\|\|\|@LB@ ACCGACG CGGCA@LB@3'-UUU...UUAGA UUUAA...GUG-5'@LB@ \| \|@LB@ 63 50@LB@@LB@@LB@Energy -10.32 kcal/mol@LB@Hybridization Energy -21.4836 kcal/mol@LB@Unfolding Energy - mRNA 6.28034 kcal/mol@LB@Unfolding Energy - sRNA 4.8826 kcal/mol@LB@@LB@Position - mRNA 270 -- 282@LB@Position - sRNA 51 -- 62@LB@Position Seed - mRNA 270 -- 276@LB@Position Seed - sRNA 56 -- 62@LB@
195	0.01197	0.2159	b0738	tolR	-7.83	0.338188	180 -- 193	26 -- 40	membrane spanning protein in TolA-TolQ-TolR complex	_____________179_____________194\n_____________\|_______________\|\n5'-UGG...UUAGC______CA_______GUAAG...UGG-3'\n______________GA_GAG__ACAAGGG\n______________\|\|_\|\|\|__\|\|\|\|\|\|\|\n______________CU_CUC__UGUUCCC\n3'-UUU...UAAUA__A___AA_______GUGUC...GUG-5'\n_____________\|_______________\|\n_____________41______________25\n	187 -- 193	26 -- 32	-14.5104 kcal/mol	3.98616 kcal/mol	2.69022 kcal/mol	179 194@LB@ \| \|@LB@5'-UGG...UUAGC CA GUAAG...UGG-3'@LB@ GA GAG ACAAGGG@LB@ \|\| \|\|\| \|\|\|\|\|\|\|@LB@ CU CUC UGUUCCC@LB@3'-UUU...UAAUA A AA GUGUC...GUG-5'@LB@ \| \|@LB@ 41 25@LB@@LB@@LB@Energy -7.83 kcal/mol@LB@Hybridization Energy -14.5104 kcal/mol@LB@Unfolding Energy - mRNA 3.98616 kcal/mol@LB@Unfolding Energy - sRNA 2.69022 kcal/mol@LB@@LB@Position - mRNA 180 -- 193@LB@Position - sRNA 26 -- 40@LB@Position Seed - mRNA 187 -- 193@LB@Position Seed - sRNA 26 -- 32@LB@
45	0.002234	0.1727	b0055	djlA	-13.22	0.033048	207 -- 250	35 -- 76	membrane-anchored DnaK co-chaperone DNA-binding protein	_____________206___________________________________________251\n_____________\|_____________________________________________\|\n5'-GAC...UGCAG_________AA____A_____GUG_______CCUUAC________CGGCG...UUG-3'\n______________UAUUGGGGA__AAUC_UUGGC___GCCGUGG_______UGAUGGG\n______________:\|:::\|\|\|\|__\|\|\|\|_\|\|\|\|\|___\|\|\|\|\|::_______\|\|\|\|\|:\|\n______________GUGGUCCCU__UUAG_AACCG___CGGCAUU_______ACUACUC\n3'-UUU...CGGUU_____________________ACG_______UAAUAAU_______AAUGU...GUG-5'\n_____________\|_____________________________________________\|\n_____________77____________________________________________34\n	244 -- 250	35 -- 41	-37.823 kcal/mol	15.2445 kcal/mol	9.3594 kcal/mol	206 251@LB@ \| \|@LB@5'-GAC...UGCAG AA A GUG CCUUAC CGGCG...UUG-3'@LB@ UAUUGGGGA AAUC UUGGC GCCGUGG UGAUGGG@LB@ :\|:::\|\|\|\| \|\|\|\| \|\|\|\|\| \|\|\|\|\|:: \|\|\|\|\|:\|@LB@ GUGGUCCCU UUAG AACCG CGGCAUU ACUACUC@LB@3'-UUU...CGGUU ACG UAAUAAU AAUGU...GUG-5'@LB@ \| \|@LB@ 77 34@LB@@LB@@LB@Energy -13.22 kcal/mol@LB@Hybridization Energy -37.823 kcal/mol@LB@Unfolding Energy - mRNA 15.2445 kcal/mol@LB@Unfolding Energy - sRNA 9.3594 kcal/mol@LB@@LB@Position - mRNA 207 -- 250@LB@Position - sRNA 35 -- 76@LB@Position Seed - mRNA 244 -- 250@LB@Position Seed - sRNA 35 -- 41@LB@
40	0.002113	0.1727	b2314	dedD	-12.04	0.056230	10 -- 25	1 -- 19	membrane-anchored periplasmic protein involved in septation	_____________9___________________26\n_____________\|___________________\|\n5'-UUG...GCGCC____GAA____________GGUGC...AUU-3'\n______________ACUG______GGGCCGCGC\n______________\|\|\|\|______:\|\|\|\|\|\|:\|\n______________UGAC______UCCGGCGUG\n3'-UUU...UGUCG____AAAAAG_________-5'\n_____________\|__________________\|\n_____________20_________________1\n	19 -- 25	1 -- 7	-21.4331 kcal/mol	3.12458 kcal/mol	6.26391 kcal/mol	9 26@LB@ \| \|@LB@5'-UUG...GCGCC GAA GGUGC...AUU-3'@LB@ ACUG GGGCCGCGC@LB@ \|\|\|\| :\|\|\|\|\|\|:\|@LB@ UGAC UCCGGCGUG@LB@3'-UUU...UGUCG AAAAAG -5'@LB@ \| \|@LB@ 20 1@LB@@LB@@LB@Energy -12.04 kcal/mol@LB@Hybridization Energy -21.4331 kcal/mol@LB@Unfolding Energy - mRNA 3.12458 kcal/mol@LB@Unfolding Energy - sRNA 6.26391 kcal/mol@LB@@LB@Position - mRNA 10 -- 25@LB@Position - sRNA 1 -- 19@LB@Position Seed - mRNA 19 -- 25@LB@Position Seed - sRNA 1 -- 7@LB@
122	0.006658	0.1913	b0600	ybdL	-10.23	0.125775	275 -- 297	68 -- 86	methionine aminotransferase PLP-dependent	_____________274_____________________298\n_____________\|_______________________\|\n5'-UCA...AGCGC___G____AG__________C__UUA-3'\n______________GCU_GCGC__CAACACCAGG_GA\n______________:\|\|_\|\|\|\|__\|\|\|\|\|\|\|\|\|\|_\|\|\n______________UGA_CGCG__GUUGUGGUCC_CU\n3'-UUU...GCUUA_______________________UUAGA...GUG-5'\n_____________\|_______________________\|\n_____________87______________________67\n	288 -- 294	70 -- 76	-27.7616 kcal/mol	10.1544 kcal/mol	7.37523 kcal/mol	274 298@LB@ \| \|@LB@5'-UCA...AGCGC G AG C UUA-3'@LB@ GCU GCGC CAACACCAGG GA@LB@ :\|\| \|\|\|\| \|\|\|\|\|\|\|\|\|\| \|\|@LB@ UGA CGCG GUUGUGGUCC CU@LB@3'-UUU...GCUUA UUAGA...GUG-5'@LB@ \| \|@LB@ 87 67@LB@@LB@@LB@Energy -10.23 kcal/mol@LB@Hybridization Energy -27.7616 kcal/mol@LB@Unfolding Energy - mRNA 10.1544 kcal/mol@LB@Unfolding Energy - sRNA 7.37523 kcal/mol@LB@@LB@Position - mRNA 275 -- 297@LB@Position - sRNA 68 -- 86@LB@Position Seed - mRNA 288 -- 294@LB@Position Seed - sRNA 70 -- 76@LB@
152	0.009044	0.2059	b1448	mnaT	-7.00	0.457084	229 -- 266	50 -- 86	methionine N-acyltransferase; L-amino acid N-acyltransferase	_____________228____________________________________267\n_____________\|______________________________________\|\n5'-CGC...AGCCG_________C_____C________AUAACCA_______UUGUA...CGG-3'\n______________ACUGUGCUG_CAUUG_GGAAAUUU_______CGCCGUG\n______________\|\|\|\|:\|\|::_\|\|:::_\|\|\|\|\|\|:\|_______\|\|\|\|\|\|:\n______________UGACGCGGU_GUGGU_CCUUUAGA_______GCGGCAU\n3'-UUU...GCUUA_________U_____C________ACCGAC________UUAAU...GUG-5'\n_____________\|______________________________________\|\n_____________87_____________________________________49\n	260 -- 266	50 -- 56	-33.8437 kcal/mol	9.99136 kcal/mol	16.8518 kcal/mol	228 267@LB@ \| \|@LB@5'-CGC...AGCCG C C AUAACCA UUGUA...CGG-3'@LB@ ACUGUGCUG CAUUG GGAAAUUU CGCCGUG@LB@ \|\|\|\|:\|\|:: \|\|::: \|\|\|\|\|\|:\| \|\|\|\|\|\|:@LB@ UGACGCGGU GUGGU CCUUUAGA GCGGCAU@LB@3'-UUU...GCUUA U C ACCGAC UUAAU...GUG-5'@LB@ \| \|@LB@ 87 49@LB@@LB@@LB@Energy -7.00 kcal/mol@LB@Hybridization Energy -33.8437 kcal/mol@LB@Unfolding Energy - mRNA 9.99136 kcal/mol@LB@Unfolding Energy - sRNA 16.8518 kcal/mol@LB@@LB@Position - mRNA 229 -- 266@LB@Position - sRNA 50 -- 86@LB@Position Seed - mRNA 260 -- 266@LB@Position Seed - sRNA 50 -- 56@LB@
90	0.004828	0.1855	b2149	mglA	-7.32	0.409352	216 -- 239	61 -- 86	methyl-galactoside ABC transporter ATPase	_____________215________________________240\n_____________\|__________________________\|\n5'-CGA...CAACG___C____CU__________UA____UUGGA...AUU-3'\n______________ACU_CGUC____CCGGGGAA__CUUG\n______________\|\|\|_\|\|:\|____\|\|:\|\|\|\|\|__\|\|\|\|\n______________UGA_GCGG____GGUCCCUU__GAAC\n3'-UUU...GCUUA___C____UUGU________UA____CGACG...GUG-5'\n_____________\|__________________________\|\n_____________87_________________________60\n	227 -- 233	67 -- 73	-23.5814 kcal/mol	8.829 kcal/mol	7.43637 kcal/mol	215 240@LB@ \| \|@LB@5'-CGA...CAACG C CU UA UUGGA...AUU-3'@LB@ ACU CGUC CCGGGGAA CUUG@LB@ \|\|\| \|\|:\| \|\|:\|\|\|\|\| \|\|\|\|@LB@ UGA GCGG GGUCCCUU GAAC@LB@3'-UUU...GCUUA C UUGU UA CGACG...GUG-5'@LB@ \| \|@LB@ 87 60@LB@@LB@@LB@Energy -7.32 kcal/mol@LB@Hybridization Energy -23.5814 kcal/mol@LB@Unfolding Energy - mRNA 8.829 kcal/mol@LB@Unfolding Energy - sRNA 7.43637 kcal/mol@LB@@LB@Position - mRNA 216 -- 239@LB@Position - sRNA 61 -- 86@LB@Position Seed - mRNA 227 -- 233@LB@Position Seed - sRNA 67 -- 73@LB@

Details of Selected Interaction Download Interaction Details

mRNA

sRNA

Energy

Hybridization Energy

Unfolding Energy - mRNA

Unfolding Energy - sRNA

Position - mRNA

Position - sRNA

Position Seed - mRNA

Position Seed - sRNA

Evolutionary conservation of mRNA targets (alignment generated with Jalview)

Evolutionary conservation of sRNA (alignment generated with Jalview)

Job resubmission

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When using CopraRNA please cite :

Patrick R. Wright, Jens Georg, Martin Mann, Dragos A. Sorescu, Andreas S. Richter, Steffen Lott, Robert Kleinkauf, Wolfgang R. Hess, and Rolf Backofen
CopraRNA and IntaRNA: predicting small RNA targets, networks and interaction domains
Nucleic Acids Research, 2014, 42 (W1), W119-W123.
Patrick R. Wright, Andreas S. Richter, Kai Papenfort, Martin Mann, Joerg Vogel, Wolfgang R. Hess, Rolf Backofen and Jens Georg
Comparative genomics boosts target prediction for bacterial small RNAs
Proc Natl Acad Sci USA, 2013, 110 (37), E3487-E3496.
Martin Raden, Syed M Ali, Omer S Alkhnbashi, Anke Busch, Fabrizio Costa, Jason A Davis, Florian Eggenhofer, Rick Gelhausen, Jens Georg, Steffen Heyne, Michael Hiller, Kousik Kundu, Robert Kleinkauf, Steffen C Lott, Mostafa M Mohamed, Alexander Mattheis, Milad Miladi, Andreas S Richter, Sebastian Will, Joachim Wolff, Patrick R Wright, and Rolf Backofen
Freiburg RNA tools: a central online resource for RNA-focused research and teaching
Nucleic Acids Research, 46(W1), W25-W29, 2018.

Results are computed with CopraRNA version 2.1.2

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